Restriction map for pF1KB7965
 (Linear) (Six-base) MAPSORT of: KIBB7965.seq  Check: 9964  from: 1  to: 840



KIBB7965.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:25  ..

AccI GT'mk_AC

Cuts at:      0     740     840 
   Size:        740     100
 
Acc65I G'GTAC_C

Cuts at:      0     663     840 
   Size:        663     177
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     214     324     840 
   Size:        214     110     516

  Fragments arranged by size: 

                 516     214     110
 
AleI CACnn'nnGTG

Cuts at:      0     763     840 
   Size:        763      77
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     430     840 
   Size:        430     410
 
AlwNI CAG_nnn'CTG

Cuts at:      0      94     840 
   Size:         94     746
 
ApaI G_GGCC'C

Cuts at:      0     772     840 
   Size:        772      68
 
ApoI r'AATT_y

Cuts at:      0      24     840 
   Size:         24     816
 
AvaI C'yCGr_G

Cuts at:      0     466     571     840 
   Size:        466     105     269

  Fragments arranged by size: 

                 466     269     105
 
BanI G'GyrC_C

Cuts at:      0     492     582     598     663     757     840 
   Size:        492      90      16      65      94      83

  Fragments arranged by size: 

                 492      94      90      83      65      16
 
BanII G_rGCy'C

Cuts at:      0     772     840 
   Size:        772      68
 
BbeI G_GCGC'C

Cuts at:      0     496     586     602     840 
   Size:        496      90      16     238

  Fragments arranged by size: 

                 496     238      90      16
 
BglI GCCn_nnn'nGGC

Cuts at:      0     490     840 
   Size:        490     350
 
BlpI GC'TnA_GC

Cuts at:      0     298     840 
   Size:        298     542
 
Bme1580I G_kGCm'C

Cuts at:      0     566     772     840 
   Size:        566     206      68

  Fragments arranged by size: 

                 566     206      68
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     244     647     840 
   Size:        244     403     193

  Fragments arranged by size: 

                 403     244     193
 
Bpu10I CC'TnA_GC

Cuts at:      0     638     840 
   Size:        638     202
 
BsaAI yAC'GTr

Cuts at:      0     380     840 
   Size:        380     460
 
BsaHI Gr'CG_yC

Cuts at:      0     493     583     599     840 
   Size:        493      90      16     241

  Fragments arranged by size: 

                 493     241      90      16
 
BsaWI w'CCGG_w

Cuts at:      0      72     263     840 
   Size:         72     191     577

  Fragments arranged by size: 

                 577     191      72
 
BseYI C'CCAG_C

Cuts at:      0      51     318     527     612     840 
   Size:         51     267     209      85     228

  Fragments arranged by size: 

                 267     228     209      85      51
 
BsiHKAI G_wGCw'C

Cuts at:      0     101     304     840 
   Size:        101     203     536

  Fragments arranged by size: 

                 536     203     101
 
BsmI GAATG_Cn'

Cuts at:      0     213     840 
   Size:        213     627
 
Bsp1286I G_dGCh'C

Cuts at:      0     101     304     566     772     840 
   Size:        101     203     262     206      68

  Fragments arranged by size: 

                 262     206     203     101      68
 
BspEI T'CCGG_A

Cuts at:      0     263     840 
   Size:        263     577
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     278     840 
   Size:        278     562
 
BsrFI r'CCGG_y

Cuts at:      0     334     666     840 
   Size:        334     332     174

  Fragments arranged by size: 

                 334     332     174
 
BssSI C'ACGA_G

Cuts at:      0     559     840 
   Size:        559     281
 
BstEII G'GTnAC_C

Cuts at:      0     360     840 
   Size:        360     480
 
BtgI C'CryG_G

Cuts at:      0     764     829     840 
   Size:        764      65      11

  Fragments arranged by size: 

                 764      65      11
 
BtsI GCAGTG_nn'

Cuts at:      0     785     840 
   Size:        785      55
 
EaeI y'GGCC_r

Cuts at:      0     420     428     678     840 
   Size:        420       8     250     162

  Fragments arranged by size: 

                 420     250     162       8
 
EarI CTCTTCn'nnn_

Cuts at:      0     132     840 
   Size:        132     708
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     247     826     840 
   Size:        247     579      14

  Fragments arranged by size: 

                 579     247      14
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     214     244     324     647     840 
   Size:        214      30      80     323     193

  Fragments arranged by size: 

                 323     214     193      80      30
 
EcoO109I rG'GnC_Cy

Cuts at:      0     514     707     769     840 
   Size:        514     193      62      71

  Fragments arranged by size: 

                 514     193      71      62
 
FspI TGC'GCA

Cuts at:      0     406     840 
   Size:        406     434
 
FspAI rTGC'GCAy

Cuts at:      0     406     840 
   Size:        406     434
 
HaeII r_GCGC'y

Cuts at:      0     496     586     602     840 
   Size:        496      90      16     238

  Fragments arranged by size: 

                 496     238      90      16
 
HincII GTy'rAC

Cuts at:      0      63     840 
   Size:         63     777
 
KasI G'GCGC_C

Cuts at:      0     492     582     598     840 
   Size:        492      90      16     242

  Fragments arranged by size: 

                 492     242      90      16
 
KpnI G_GTAC'C

Cuts at:      0     667     840 
   Size:        667     173
 
MscI TGG'CCA

Cuts at:      0     430     680     840 
   Size:        430     250     160

  Fragments arranged by size: 

                 430     250     160
 
MslI CAynn'nnrTG

Cuts at:      0     763     840 
   Size:        763      77
 
MspA1I CmG'CkG

Cuts at:      0       7     168     276     722     755     794     840 
   Size:          7     161     108     446      33      39      46

  Fragments arranged by size: 

                 446     161     108      46      39      33       7
 
NaeI GCC'GGC

Cuts at:      0     336     840 
   Size:        336     504
 
NarI GG'CG_CC

Cuts at:      0     493     583     599     840 
   Size:        493      90      16     241

  Fragments arranged by size: 

                 493     241      90      16
 
NgoMIV G'CCGG_C

Cuts at:      0     334     840 
   Size:        334     506
 
PmlI CAC'GTG

Cuts at:      0     380     840 
   Size:        380     460
 
PpuMI rG'GwC_Cy

Cuts at:      0     514     840 
   Size:        514     326
 
PspOMI G'GGCC_C

Cuts at:      0     768     840 
   Size:        768      72
 
PstI C_TGCA'G

Cuts at:      0     275     840 
   Size:        275     565
 
PvuII CAG'CTG

Cuts at:      0       7     168     840 
   Size:          7     161     672

  Fragments arranged by size: 

                 672     161       7
 
SanDI GG'GwC_CC

Cuts at:      0     514     840 
   Size:        514     326
 
SapI GCTCTTCn'nnn_

Cuts at:      0     132     840 
   Size:        132     708
 
SfcI C'TryA_G

Cuts at:      0     271     840 
   Size:        271     569
 
SfoI GGC'GCC

Cuts at:      0     494     584     600     840 
   Size:        494      90      16     240

  Fragments arranged by size: 

                 494     240      90      16
 
SmaI CCC'GGG

Cuts at:      0     468     573     840 
   Size:        468     105     267

  Fragments arranged by size: 

                 468     267     105
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     805     837     840 
   Size:        805      32       3

  Fragments arranged by size: 

                 805      32       3
 
XmaI C'CCGG_G

Cuts at:      0     466     571     840 
   Size:        466     105     269

  Fragments arranged by size: 

                 466     269     105
 

 Enzymes that do cut:

     AccI   Acc65I     AcuI     AleI     AlfI    AlwNI     ApaI     ApoI
     AvaI     BanI    BanII     BbeI     BglI     BlpI Bme1580I     BpmI
   Bpu10I    BsaAI    BsaHI    BsaWI    BseYI  BsiHKAI     BsmI Bsp1286I
    BspEI    BspMI    BsrFI    BssSI   BstEII     BtgI     BtsI     EaeI
     EarI     EciI  Eco57MI EcoO109I     FspI    FspAI    HaeII   HincII
     KasI     KpnI     MscI     MslI   MspA1I     NaeI     NarI   NgoMIV
     PmlI    PpuMI   PspOMI     PstI    PvuII    SanDI     SapI     SfcI
     SfoI     SmaI     TstI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AloI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII    BmgBI
     BmrI     BmtI     BplI    BpuEI     BsaI    BsaBI    BsaXI    BseRI
     BsgI    BsiEI    BsiWI    BsmBI    BspHI    BsrBI    BsrDI    BsrGI
   BssHII   BstAPI    BstBI    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI
     ClaI    CspCI     DraI   DraIII     DrdI     EagI  EcoICRI    EcoNI
    EcoRI    EcoRV     FalI     FseI    Hin4I  HindIII     HpaI     MfeI
     MluI     MmeI     NcoI     NdeI     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PpiI
    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII     SacI    SacII
     SalI     SbfI     ScaI    SexAI     SfiI     SgfI    SgrAI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI
    TaqII     TatI  Tth111I     XbaI     XcmI     XhoI     XmnI     ZraI
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