Restriction map for pF1KB7934
##################################
# NO CUT ENZYMES (  192 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AfaI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwNI       AocI        
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AvaI        
AvaII       AviII       AvrII       BalI        BanI        
BanII       BbeI        BbrPI       BbvCI       BciVI       
BclI        BcnI        BfaI        BfrI        BglI        
BglII       BlnI        BlpI        BpmI        Bpu1102I    
BsaAI       BsaBI       BsaHI       BsaI        BsaJI       
BsaWI       BseAI       BsgI        BsiEI       BsiWI       
BsmBI       BsmFI       BsmI        BsoBI       Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstXI       BstZ17I     Bsu36I      
Cac8I       CelII       CfoI        Cfr10I      ClaI        
CpoI        DraI        DraII       DraIII      DrdI        
DsaI        EaeI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoO109I    EcoO65I     
EcoRI       EcoRV       EcoT14I     FauI        FbaI        
FseI        FspI        HaeII       HapII       HgaI        
HhaI        Hin1I       HinP1I      HindIII     HinfI       
HpaI        HpaII       KasI        KpnI        KspI        
MaeI        MaeII       MaeIII      MamI        MfeI        
MluI        MluNI       MroI        MscI        MseI        
MspA1I      MspI        MunI        MvnI        NaeI        
NarI        NciI        NcoI        NgoMIV      NheI        
NotI        NruI        NspI        NspV        PacI        
PaeR7I      PflMI       PinAI       PleI        PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        PvuII       RcaI        RsaI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StyI        SwaI        TfiI        Tru9I       Tsp45I      
Van91I      XbaI        XcmI        XhoI        XmaCI       
XmaI        XmnI        


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# ONE CUT ENZYMES (   42 enzymes) #
###################################

AciI          196  GCGG                 CCGC(-3/-1)
AcsI          175  AAATTT               [GA]^AATT[CT]
ApoI          175  AAATTT               [GA]^AATT[CT]
AspHI         132  GTGCTC               G[AT]GC[AT]^C
AspI          150  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI         270  GGATCC               G^GATCC
BbsI          147  GAAGAC               GAAGAC(2/6)
BcgI           60  GCAAACTTGTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BmyI          132  GTGCTC               G[AGT]GC[ACT]^C
BpuAI         147  GAAGAC               GAAGAC(2/6)
BsiHKAI       132  GTGCTC               G[AT]GC[AT]^C
BsiYI         238  CCTCCCAAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          238  CCTCCCAAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI         250  GAGAC                GTCTC(1/5)
Bsp1286I      132  GTGCTC               G[AGT]GC[ACT]^C
BsrI          384  ACTGG                ACTGG(1/-1)
BssKI         352  CCTGG                ^CC[ACGT]GG
BstNI         352  CCTGG                CC^[AT]GG
Cfr13I        204  GGCCC                G^G[ACGT]CC
EcoNI         238  CCTCCCAAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRII        352  CCTGG                ^CC[AT]GG
EcoT22I        47  ATGCAT               ATGCA^T
HincII        106  GTCAAC               GT[CT]^[GA]AC
HindII        106  GTCAAC               GT[CT]^[GA]AC
HphI          367  GGTGA                GGTGA(8/7)
MslI           91  CACCCAGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI          352  CCTGG                CC^[AT]GG
NdeI           44  CATATG               CA^TATG
NlaIII        153  CATG                 CATG^
NlaIV         270  GGATCC               GG[ACGT]^[ACGT]CC
NsiI           47  ATGCAT               ATGCA^T
PflFI         150  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PstI          410  CTGCAG               CTGCA^G
Sau96I        204  GGCCC                G^G[ACGT]CC
ScrFI         352  CCTGG                CC^[ACGT]GG
SfcI          410  CTGCAG               C^T[GA][CT]AG
StuI          289  AGGCCT               AGG^CCT
TaqI          486  TCGA                 T^CGA
Tsp509I       176  AATT                 ^AATT
TspRI         405  CACTG                CA[GC]TG[ACGT][ACGT]^
Tth111I       150  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I       486  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

AluI          449  AGCT                 AG^CT
              489  AGCT                
BstF5I        349  CATCC                GGATG(2/0)
              585  GGATG               
BstYI          12  AGATCC               [GA]^GATC[CT]
              270  GGATCC              
DpnII          13  GATC                 ^GATC
              271  GATC                
FokI          349  CATCC                GGATG(9/13)
              585  GGATG               
MboI           13  GATC                 ^GATC
              271  GATC                
MflI           12  AGATCC               [GA]^GATC[CT]
              270  GGATCC              
MwoI          196  GCGGACAAGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              481  GCATATCGAGC         
NdeII          13  GATC                 ^GATC
              271  GATC                
Sau3AI         13  GATC                 ^GATC
              271  GATC                
SfaNI          97  GATGC                GCATC(5/9)
              505  GATGC               
XhoII          12  AGATCC               [GA]^GATC[CT]
              270  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   12 enzymes) #
#########################################

AlwI        BbvI        BseRI       DdeI        EarI        
Fnu4HI      HaeIII      ItaI        Ksp632I     MboII       
MnlI        TseI        
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