Restriction map for pF1KB7928
##################################
# NO CUT ENZYMES (  163 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AclI        
AcsI        AcyI        AfaI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwNI       AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AviII       AvrII       BamHI       
BanI        BbeI        BbrPI       BbvCI       BcgI        
BciVI       BfrI        BglI        BglII       BlnI        
BlpI        Bpu1102I    BsaAI       BsaBI       BsaHI       
BsaI        BsgI        BsiEI       BsiHKAI     BsiWI       
BsmAI       BsmBI       BsmI        Bsp1407I    BspDI       
BspHI       BspLU11I    BspMI       BsrDI       BsrGI       
BssHII      BssSI       Bst1107I    BstBI       BstEII      
BstPI       BstUI       BstZ17I     Bsu36I      CelII       
CfoI        ClaI        CpoI        DraI        DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO65I     EcoRI       
EcoRV       EcoT22I     FseI        FspI        HaeII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HpaI        KasI        KpnI        
KspI        MaeII       MaeIII      MamI        MfeI        
MluI        MseI        MunI        MvnI        NaeI        
NarI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsaI        RsrII       
SacI        SacII       SalI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        Tru9I       
Tsp45I      Tsp509I     Tth111I     Van91I      XbaI        
XcmI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   33 enzymes) #
###################################

AccIII        325  TCCGGA               T^CCGGA
BalI          341  TGGCCA               TGG^CCA
BbsI          489  GAAGAC               GAAGAC(2/6)
BclI          251  TGATCA               T^GATCA
BpuAI         489  GAAGAC               GAAGAC(2/6)
BsaWI         325  TCCGGA               [AT]^CCGG[AT]
BseAI         325  TCCGGA               T^CCGGA
BseRI         409  GAGGAG               GAGGAG(10/8)
BsmFI         236  GGGAC                GGGAC(10/14)
BspEI         325  TCCGGA               T^CCGGA
BsrBI          24  CCGCTC               CCGCTC(-3/-3)
BsrFI         225  GCCGGT               [GA]^CCGG[CT]
BstAPI         50  GCAACTGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I        300  CATCC                GGATG(2/0)
Cfr10I        225  GCCGGT               [GA]^CCGG[CT]
DdeI          535  CTGAG                C^T[ACGT]AG
DsaI          368  CCATGG               C^C[GA][CT]GG
EaeI          341  TGGCCA               [CT]^GGCC[GA]
EcoT14I       368  CCATGG               C^C[AT][AT]GG
FbaI          251  TGATCA               T^GATCA
FokI          300  CATCC                GGATG(9/13)
MluNI         341  TGGCCA               TGG^CCA
MroI          325  TCCGGA               T^CCGGA
MscI          341  TGGCCA               TGG^CCA
MslI           73  CATCGGAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI          368  CCATGG               C^CATGG
PpuMI         236  GGGACCC              [GA]G^G[AT]CC[CT]
PstI          151  CTGCAG               CTGCA^G
SfaNI         263  GCATC                GCATC(5/9)
SmaI          117  CCCGGG               CCC^GGG
StyI          368  CCATGG               C^C[AT][AT]GG
XmaCI         117  CCCGGG               C^CCGGG
XmaI          117  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AvaI          117  CCCGGG               C^[CT]CG[GA]G
              574  CCCGAG              
AvaII          63  GGACC                G^G[AT]CC
              237  GGACC               
BanII          82  GGGCTC               G[GA]GC[CT]^C
              537  GAGCCC              
BcnI          117  CCCGG                CC^[GC]GG
              118  CCGGG               
BfaI          220  CTAG                 C^TAG
              466  CTAG                
BpmI           85  CTCCAG               CTGGAG(16/14)
              514  CTGGAG              
BsiYI          60  CCTGGACCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              438  CCGAGAGCTGG         
BslI           60  CCTGGACCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              438  CCGAGAGCTGG         
BsoBI         117  CCCGGG               C^[CT]CG[GA]G
              574  CCCGAG              
BsrI            6  CCAGT                ACTGG(1/-1)
              525  CCAGT               
DraII         236  GGGACCC              [GA]G^G[ACGT]CC[CT]
              578  AGGCCCC             
EarI          307  CTCTTC               CTCTTC(1/4)
              544  GAAGAG              
EcoO109I      236  GGGACCC              [GA]G^G[ACGT]CC[CT]
              578  AGGCCCC             
FauI          101  GCGGG                CCCGC(4/6)
              234  GCGGG               
HphI          378  GGTGA                GGTGA(8/7)
               28  TCACC               
Ksp632I       307  CTCTTC               CTCTTC(1/4)
              544  GAAGAG              
MaeI          220  CTAG                 C^TAG
              466  CTAG                
NciI          117  CCCGG                CC^[GC]GG
              118  CCGGG               
NlaIII        291  CATG                 CATG^
              369  CATG                
PvuII         148  CAGCTG               CAG^CTG
              511  CAGCTG              
SapI          544  GAAGAGC              GCTCTTC(1/4)
              306  GCTCTTC             
SfcI          128  CTACAG               C^T[GA][CT]AG
              151  CTGCAG              
TspRI         131  CAGTG                CA[GC]TG[ACGT][ACGT]^
              318  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   39 enzymes) #
#########################################

AciI        AluI        AlwI        BbvI        BmyI        
BsaJI       Bsp1286I    BssKI       BstNI       BstXI       
BstYI       Cac8I       Cfr13I      DpnII       EcoRII      
Fnu4HI      HaeIII      HapII       HinfI       HpaII       
ItaI        MboI        MboII       MflI        MnlI        
MspA1I      MspI        MvaI        MwoI        NdeII       
NlaIV       Sau3AI      Sau96I      ScrFI       TaqI        
TfiI        TseI        TthHB8I     XhoII       
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