Restriction map for pF1KB7909
##################################
# NO CUT ENZYMES (  164 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwNI       Aor51HI     ApaI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AvaI        AviII       AvrII       BalI        BanI        
BbeI        BbrPI       BbvCI       BcgI        BclI        
BfrI        BglII       BlnI        BpmI        BsaAI       
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BseRI       BsiHKAI     BsiWI       BsmAI       BsmBI       
BsmI        BsoBI       Bsp1407I    BspEI       BspHI       
BspLU11I    BsrBI       BsrDI       BsrFI       BsrGI       
BsrI        BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstZ17I     Cfr10I      CpoI        
DraI        DraIII      DrdI        Eam1105I    Eco47III    
EcoO65I     EcoRI       EcoRV       EcoT14I     EcoT22I     
FbaI        FseI        FspI        HaeII       Hin1I       
HincII      HindII      HindIII     HpaI        KasI        
KpnI        KspI        MaeII       MaeIII      MamI        
MfeI        MluI        MluNI       MroI        MscI        
MseI        MunI        NaeI        NarI        NcoI        
NdeI        NgoMIV      NheI        NlaIII      NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PstI        PvuI        RcaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfaNI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        StyI        SwaI        Tru9I       Tsp45I      
Tsp509I     Tth111I     Van91I      XbaI        XcmI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AfaI          204  GTAC                 GT^AC
AocI          109  CCTCAGG              CC^T[ACGT]AGG
AvaII         378  GGTCC                G^G[AT]CC
BanII         283  GAGCCC               G[GA]GC[CT]^C
BbsI          228  GAAGAC               GAAGAC(2/6)
BciVI         390  GGATAC               GTATCC(6/5)
BcnI           47  CCCGG                CC^[GC]GG
BfaI           43  CTAG                 C^TAG
BlpI          273  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      273  GCTGAGC              GC^T[ACGT]AGC
BpuAI         228  GAAGAC               GAAGAC(2/6)
BsgI          178  CTGCAC               GTGCAG(16/14)
BsiEI          73  CGGCCG               CG[GA][CT]^CG
BsmFI         379  GTCCC                GGGAC(10/14)
BspDI         211  ATCGAT               AT^CGAT
BspMI         161  ACCTGC               ACCTGC(4/8)
BssHII         23  GCGCGC               G^CGCGC
BstF5I         84  GGATG                GGATG(2/0)
BstXI         341  CCAGGCGGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        109  CCTCAGG              CC^T[ACGT]AGG
CelII         273  GCTGAGC              GC^T[ACGT]AGC
Cfr13I        378  GGTCC                G^G[ACGT]CC
ClaI          211  ATCGAT               AT^CGAT
DraII         377  AGGTCCC              [GA]G^G[ACGT]CC[CT]
DsaI          359  CCACGG               C^C[GA][CT]GG
EaeI           73  CGGCCG               [CT]^GGCC[GA]
EagI           73  CGGCCG               C^GGCCG
EarI          298  GAAGAG               CTCTTC(1/4)
EclXI          73  CGGCCG               C^GGCCG
Eco52I         73  CGGCCG               C^GGCCG
Eco81I        109  CCTCAGG              CC^T[ACGT]AGG
EcoNI         194  CCTGCGCGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      377  AGGTCCC              [GA]G^G[ACGT]CC[CT]
FokI           84  GGATG                GGATG(9/13)
HgaI          278  GCGTC                GACGC(5/10)
HphI           93  TCACC                GGTGA(8/7)
Ksp632I       298  GAAGAG               CTCTTC(1/4)
MaeI           43  CTAG                 C^TAG
MslI          360  CACGGAAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI           47  CCCGG                CC^[GC]GG
PpuMI         377  AGGTCCC              [GA]G^G[AT]CC[CT]
PvuII         271  CAGCTG               CAG^CTG
RsaI          204  GTAC                 GT^AC
Sau96I        378  GGTCC                G^G[ACGT]CC
SfcI          288  CTACAG               C^T[GA][CT]AG


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

AluI          101  AGCT                 AG^CT
              272  AGCT                
BamHI         115  GGATCC               G^GATCC
              325  GGATCC              
BglI           14  GCCGGACAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              336  GCCCCCCAGGC         
BstNI         166  CCAGG                CC^[AT]GG
              341  CCAGG               
BstYI         115  GGATCC               [GA]^GATC[CT]
              325  GGATCC              
EcoRII        166  CCAGG                ^CC[AT]GG
              341  CCAGG               
FauI           11  CCCGC                CCCGC(4/6)
              316  GCGGG               
HinfI          90  GATTC                G^A[ACGT]TC
              214  GATTC               
MboII         228  GAAGA                GAAGA(8/7)
              298  GAAGA               
MflI          115  GGATCC               [GA]^GATC[CT]
              325  GGATCC              
MvaI          166  CCAGG                CC^[AT]GG
              341  CCAGG               
TfiI           90  GATTC                G^A[AT]TC
              214  GATTC               
TspRI         353  CAGTG                CA[GC]TG[ACGT][ACGT]^
              372  CACTG               
XhoII         115  GGATCC               [GA]^GATC[CT]
              325  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   35 enzymes) #
#########################################

AccII       AciI        AlwI        BbvI        BmyI        
BsaJI       BsiYI       BslI        Bsp1286I    BssKI       
BstUI       Cac8I       CfoI        DdeI        DpnII       
Fnu4HI      HaeIII      HapII       HhaI        HinP1I      
HpaII       ItaI        MboI        MnlI        MspA1I      
MspI        MvnI        MwoI        NdeII       NlaIV       
Sau3AI      ScrFI       TaqI        TseI        TthHB8I     
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