Restriction map for pF1KF0343
 (Linear) (Six-base) MAPSORT of: FLJ00343.seq  Check: 2228  from: 1  to: 7917



FLJ00343.orf

 With 182 enzymes: SgfI * 

                        September 25, 2009 15:22  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    3732    7917 
   Size:       3732    4185
 
AatII G_ACGT'C

Cuts at:      0    2411    2726    7187    7917 
   Size:       2411     315    4461     730

  Fragments arranged by size: 

                4461    2411     730     315
 
AccI GT'mk_AC

Cuts at:      0      62    2321    4499    4810    7580    7715    7917 
   Size:         62    2259    2178     311    2770     135     202

  Fragments arranged by size: 

                2770    2259    2178     311     202     135      62
 
Acc65I G'GTAC_C

Cuts at:      0    4587    7917 
   Size:       4587    3330
 
AclI AA'CG_TT

Cuts at:      0    6440    7917 
   Size:       6440    1477
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     190     421     546     826    1564    2259    5992    6413 
   Size:        190     231     125     280     738     695    3733     421

Cuts at:   6413    6749    7917 
   Size:        336    1168

  Fragments arranged by size: 

                3733    1168     738     695     421     336     280     231
                 190     125
 
AflIII A'CryG_T

Cuts at:      0     317    3361    7917 
   Size:        317    3044    4556

  Fragments arranged by size: 

                4556    3044     317
 
AleI CACnn'nnGTG

Cuts at:      0    4675    7858    7917 
   Size:       4675    3183      59

  Fragments arranged by size: 

                4675    3183      59
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    4033    7756    7917 
   Size:       4033    3723     161

  Fragments arranged by size: 

                4033    3723     161
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2713    2745    5786    5818    7917 
   Size:       2713      32    3041      32    2099

  Fragments arranged by size: 

                3041    2713    2099      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1231    4948    5244    6758    7917 
   Size:       1231    3717     296    1514    1159

  Fragments arranged by size: 

                3717    1514    1231    1159     296
 
ApaI G_GGCC'C

Cuts at:      0     591    1564    1750    2638    3031    3119    3319    4183 
   Size:        591     973     186     888     393      88     200     864

Cuts at:   4183    4367    4469    4553    5292    5689    6235    6671    6712 
   Size:        184     102      84     739     397     546     436      41

Cuts at:   6712    6808    7623    7917 
   Size:         96     815     294

  Fragments arranged by size: 

                 973     888     864     815     739     591     546     436
                 397     393     294     200     186     184     102      96
                  88      84      41
 
ApaLI G'TGCA_C

Cuts at:      0    1366    4932    5031    5040    7078    7917 
   Size:       1366    3566      99       9    2038     839

  Fragments arranged by size: 

                3566    2038    1366     839      99       9
 
ApoI r'AATT_y

Cuts at:      0    6748    7917 
   Size:       6748    1169
 
AvaI C'yCGr_G

Cuts at:      0      17     331     618     730    1042    1307    1437    2519 
   Size:         17     314     287     112     312     265     130    1082

Cuts at:   2519    3033    3334    3391    3502    3555    3676    3845    4067 
   Size:        514     301      57     111      53     121     169     222

Cuts at:   4067    4657    5825    5946    6764    7157    7623    7917 
   Size:        590    1168     121     818     393     466     294

  Fragments arranged by size: 

                1168    1082     818     590     514     466     393     314
                 312     301     294     287     265     222     169     130
                 121     121     112     111      57      53      17
 
AvrII C'CTAG_G

Cuts at:      0    6672    7917 
   Size:       6672    1245
 
BamHI G'GATC_C

Cuts at:      0     524    2859    4755    7917 
   Size:        524    2335    1896    3162

  Fragments arranged by size: 

                3162    2335    1896     524
 
BanI G'GyrC_C

Cuts at:      0     595    1711    3045    3280    4038    4102    4258    4513 
   Size:        595    1116    1334     235     758      64     156     255

Cuts at:   4513    4587    5452    6942    7198    7354    7446    7911    7917 
   Size:         74     865    1490     256     156      92     465       6

  Fragments arranged by size: 

                1490    1334    1116     865     758     595     465     256
                 255     235     156     156      92      74      64       6
            
 
BanII G_rGCy'C

Cuts at:      0     591     819     878    1105    1178    1564    1750    2351 
   Size:        591     228      59     227      73     386     186     601

Cuts at:   2351    2366    2603    2638    2878    3031    3119    3250    3319 
   Size:         15     237      35     240     153      88     131      69

Cuts at:   3319    3392    3852    4183    4367    4378    4469    4553    4600 
   Size:         73     460     331     184      11      91      84      47

Cuts at:   4600    4664    5292    5689    5963    6215    6235    6426    6649 
   Size:         64     628     397     274     252      20     191     223

Cuts at:   6649    6671    6690    6712    6808    7098    7623    7917 
   Size:         22      19      22      96     290     525     294

  Fragments arranged by size: 

                 628     601     591     525     460     397     386     331
                 294     290     274     252     240     237     228     227
                 223     191     186     184     153     131      96      91
                  88      84      73      73      69      64      59      47
                  35      22      22      20      19      15      11
 
BbeI G_GCGC'C

Cuts at:      0     599    3284    7917 
   Size:        599    2685    4633

  Fragments arranged by size: 

                4633    2685     599
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1961    2056    3801    4783    7917 
   Size:       1961      95    1745     982    3134

  Fragments arranged by size: 

                3134    1961    1745     982      95
 
BbvCI CC'TCA_GC

Cuts at:      0    1295    1966    2911    6460    7917 
   Size:       1295     671     945    3549    1457

  Fragments arranged by size: 

                3549    1457    1295     945     671
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     171     205     366     400    1677    1711    2640    2674 
   Size:        171      34     161      34    1277      34     929      34

Cuts at:   2674    7917 
   Size:       5243

  Fragments arranged by size: 

                5243    1277     929     171     161      34      34      34
                  34
 
BclI T'GATC_A

Cuts at:      0     722    4988    6089    7917 
   Size:        722    4266    1101    1828

  Fragments arranged by size: 

                4266    1828    1101     722
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1450    3889    6521    7060    7418    7917 
   Size:       1450    2439    2632     539     358     499

  Fragments arranged by size: 

                2632    2439    1450     539     499     358
 
BglII A'GATC_T

Cuts at:      0    1214    3587    6821    7917 
   Size:       1214    2373    3234    1096

  Fragments arranged by size: 

                3234    2373    1214    1096
 
BlpI GC'TnA_GC

Cuts at:      0     250    6214    7675    7917 
   Size:        250    5964    1461     242

  Fragments arranged by size: 

                5964    1461     250     242
 
Bme1580I G_kGCm'C

Cuts at:      0     591     618    1285    1370    1391    1564    1714    1750 
   Size:        591      27     667      85      21     173     150      36

Cuts at:   1750    1822    2210    2433    2638    3031    3050    3119    3152 
   Size:         72     388     223     205     393      19      69      33

Cuts at:   3152    3319    3383    3722    4022    4043    4183    4367    4469 
   Size:        167      64     339     300      21     140     184     102

Cuts at:   4469    4538    4553    4913    4936    5035    5044    5171    5292 
   Size:         69      15     360      23      99       9     127     121

Cuts at:   5292    5689    6014    6235    6601    6671    6712    6808    7082 
   Size:        397     325     221     366      70      41      96     274

Cuts at:   7082    7359    7414    7608    7623    7916    7917 
   Size:        277      55     194      15     293       1

  Fragments arranged by size: 

                 667     591     397     393     388     366     360     339
                 325     300     293     277     274     223     221     205
                 194     184     173     167     150     140     127     121
                 102      99      96      85      72      70      69      69
                  64      55      41      36      33      27      23      21
                  21      19      15      15       9       1
 
BmgBI CAC'GTC

Cuts at:      0    1371    1815    3876    3996    4494    4833    5030    5073 
   Size:       1371     444    2061     120     498     339     197      43

Cuts at:   5073    5802    6278    7917 
   Size:        729     476    1639

  Fragments arranged by size: 

                2061    1639    1371     729     498     476     444     339
                 197     120      43
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     645     780    1172    7917 
   Size:        645     135     392    6745

  Fragments arranged by size: 

                6745     645     392     135
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    3609    4200    7917 
   Size:       3609     591    3717

  Fragments arranged by size: 

                3717    3609     591
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1192    1321    1453    1777    2456    3529    6286    6739 
   Size:       1192     129     132     324     679    1073    2757     453

Cuts at:   6739    6755    6800    7120    7917 
   Size:         16      45     320     797

  Fragments arranged by size: 

                2757    1192    1073     797     679     453     324     320
                 132     129      45      16
 
Bpu10I CC'TnA_GC

Cuts at:      0     211    1295    1966    2911    3495    4372    6460    7917 
   Size:        211    1084     671     945     584     877    2088    1457

  Fragments arranged by size: 

                2088    1457    1084     945     877     671     584     211
            
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     331    2335    7819    7917 
   Size:        331    2004    5484      98

  Fragments arranged by size: 

                5484    2004     331      98
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     913    3910    4129    5240    6135    7917 
   Size:        913    2997     219    1111     895    1782

  Fragments arranged by size: 

                2997    1782    1111     913     895     219
 
BsaAI yAC'GTr

Cuts at:      0     957    1119    1664    1938    2032    2205    3378    3459 
   Size:        957     162     545     274      94     173    1173      81

Cuts at:   3459    4808    4824    5166    5994    6063    6348    6636    7689 
   Size:       1349      16     342     828      69     285     288    1053

Cuts at:   7689    7800    7917 
   Size:        111     117

  Fragments arranged by size: 

                1349    1173    1053     957     828     545     342     288
                 285     274     173     162     117     111      94      81
                  69      16
 
BsaBI GATnn'nnATC

Cuts at:      0    5503    7917 
   Size:       5503    2414
 
BsaHI Gr'CG_yC

Cuts at:      0      59      74     596     650    1139    1355    1766    2252 
   Size:         59      15     522      54     489     216     411     486

Cuts at:   2252    2408    2612    2723    2837    3281    3842    4721    4922 
   Size:        156     204     111     114     444     561     879     201

Cuts at:   4922    6417    6968    7184    7643    7917 
   Size:       1495     551     216     459     274

  Fragments arranged by size: 

                1495     879     561     551     522     489     486     459
                 444     411     274     216     216     204     201     156
                 114     111      59      54      15
 
BsaWI w'CCGG_w

Cuts at:      0    3766    3862    7917 
   Size:       3766      96    4055

  Fragments arranged by size: 

                4055    3766      96
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     433     463    2182    2212    2930    2960    7366    7396 
   Size:        433      30    1719      30     718      30    4406      30

Cuts at:   7396    7450    7480    7917 
   Size:         54      30     437

  Fragments arranged by size: 

                4406    1719     718     437     433      54      30      30
                  30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     484     487     496     748     997    1588    3988    4993 
   Size:        484       3       9     252     249     591    2400    1005

Cuts at:   4993    5971    7117    7795    7917 
   Size:        978    1146     678     122

  Fragments arranged by size: 

                2400    1146    1005     978     678     591     484     252
                 249     122       9       3
 
BseYI C'CCAG_C

Cuts at:      0     705    1824    3523    3730    4021    4537    5120    5200 
   Size:        705    1119    1699     207     291     516     583      80

Cuts at:   5200    5476    6235    6703    6808    7314    7610    7917 
   Size:        276     759     468     105     506     296     307

  Fragments arranged by size: 

                1699    1119     759     705     583     516     506     468
                 307     296     291     276     207     105      80
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     421    3059    3967    5239    5476    5694    6300    7917 
   Size:        421    2638     908    1272     237     218     606    1617

  Fragments arranged by size: 

                2638    1617    1272     908     606     421     237     218
            
 
BsiEI CG_ry'CG

Cuts at:      0     585     971    3614    4235    7067    7917 
   Size:        585     386    2643     621    2832     850

  Fragments arranged by size: 

                2832    2643     850     621     585     386
 
BsiHKAI G_wGCw'C

Cuts at:      0     167     251     764    1076    1370    3496    4360    4936 
   Size:        167      84     513     312     294    2126     864     576

Cuts at:   4936    5035    5044    5165    6032    7082    7883    7917 
   Size:         99       9     121     867    1050     801      34

  Fragments arranged by size: 

                2126    1050     867     864     801     576     513     312
                 294     167     121      99      84      34       9
 
BsmI GAATG_Cn'

Cuts at:      0    5774    7917 
   Size:       5774    2143
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    4975    5899    6013    7540    7917 
   Size:       4975     924     114    1527     377

  Fragments arranged by size: 

                4975    1527     924     377     114
 
Bsp1286I G_dGCh'C

Cuts at:      0     167     251     591     618     764     819     878    1076 
   Size:        167      84     340      27     146      55      59     198

Cuts at:   1076    1105    1178    1285    1370    1391    1564    1714    1750 
   Size:         29      73     107      85      21     173     150      36

Cuts at:   1750    1822    2210    2351    2366    2433    2603    2638    2878 
   Size:         72     388     141      15      67     170      35     240

Cuts at:   2878    3031    3050    3119    3152    3250    3319    3383    3392 
   Size:        153      19      69      33      98      69      64       9

Cuts at:   3392    3496    3722    3852    4022    4043    4183    4360    4367 
   Size:        104     226     130     170      21     140     177       7

Cuts at:   4367    4378    4469    4538    4553    4600    4664    4913    4936 
   Size:         11      91      69      15      47      64     249      23

Cuts at:   4936    5035    5044    5165    5171    5292    5689    5963    6014 
   Size:         99       9     121       6     121     397     274      51

Cuts at:   6014    6032    6215    6235    6426    6601    6649    6671    6690 
   Size:         18     183      20     191     175      48      22      19

Cuts at:   6690    6712    6808    7082    7098    7359    7414    7608    7623 
   Size:         22      96     274      16     261      55     194      15

Cuts at:   7623    7883    7916    7917 
   Size:        260      33       1

  Fragments arranged by size: 

                 397     388     340     274     274     261     260     249
                 240     226     198     194     191     183     177     175
                 173     170     170     167     153     150     146     141
                 140     130     121     121     107     104      99      98
                  96      91      85      84      73      72      69      69
                  69      67      64      64      59      55      55      51
                  48      47      36      35      33      33      29      27
                  23      22      22      21      21      20      19      19
                  18      16      15      15      15      11       9       9
                   7       6       1
 
BspHI T'CATG_A

Cuts at:      0     770    2607    4626    7917 
   Size:        770    1837    2019    3291

  Fragments arranged by size: 

                3291    2019    1837     770
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     206    3732    3759    4440    5756    7276    7838    7917 
   Size:        206    3526      27     681    1316    1520     562      79

  Fragments arranged by size: 

                3526    1520    1316     681     562     206      79      27
            
 
BsrBI CCG'CTC

Cuts at:      0     581    3513    7917 
   Size:        581    2932    4404

  Fragments arranged by size: 

                4404    2932     581
 
BsrDI GCAATG_nn'

Cuts at:      0    2187    2490    4444    6791    7347    7917 
   Size:       2187     303    1954    2347     556     570

  Fragments arranged by size: 

                2347    2187    1954     570     556     303
 
BsrFI r'CCGG_y

Cuts at:      0      84    1381    2217    3283    7808    7917 
   Size:         84    1297     836    1066    4525     109

  Fragments arranged by size: 

                4525    1297    1066     836     109      84
 
BsrGI T'GTAC_A

Cuts at:      0    1514    3620    3947    7917 
   Size:       1514    2106     327    3970

  Fragments arranged by size: 

                3970    2106    1514     327
 
BssSI C'ACGA_G

Cuts at:      0    2359    5494    7335    7558    7917 
   Size:       2359    3135    1841     223     359

  Fragments arranged by size: 

                3135    2359    1841     359     223
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    5039    6036    7917 
   Size:       5039     997    1881

  Fragments arranged by size: 

                5039    1881     997
 
BstBI TT'CG_AA

Cuts at:      0    1697    7917 
   Size:       1697    6220
 
BstEII G'GTnAC_C

Cuts at:      0    1548    3132    6285    6507    7365    7917 
   Size:       1548    1584    3153     222     858     552

  Fragments arranged by size: 

                3153    1584    1548     858     552     222
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    3024    3436    3640    3781    4476    6084    6243    7210 
   Size:       3024     412     204     141     695    1608     159     967

Cuts at:   7210    7653    7668    7917 
   Size:        443      15     249

  Fragments arranged by size: 

                3024    1608     967     695     443     412     249     204
                 159     141      15
 
BstYI r'GATC_y

Cuts at:      0     128     524    1214    2859    3587    4755    4877    5910 
   Size:        128     396     690    1645     728    1168     122    1033

Cuts at:   5910    6821    7226    7784    7917 
   Size:        911     405     558     133

  Fragments arranged by size: 

                1645    1168    1033     911     728     690     558     405
                 396     133     128     122
 
BstZ17I GTA'TAC

Cuts at:      0    2322    4811    7917 
   Size:       2322    2489    3106

  Fragments arranged by size: 

                3106    2489    2322
 
Bsu36I CC'TnA_GG

Cuts at:      0    3900    5338    5409    7089    7415    7917 
   Size:       3900    1438      71    1680     326     502

  Fragments arranged by size: 

                3900    1680    1438     502     326      71
 
BtgI C'CryG_G

Cuts at:      0     118    1268    1346    1631    2234    3633    3933    3989 
   Size:        118    1150      78     285     603    1399     300      56

Cuts at:   3989    4080    4694    5604    6662    7442    7917 
   Size:         91     614     910    1058     780     475

  Fragments arranged by size: 

                1399    1150    1058     910     780     614     603     475
                 300     285     118      91      78      56
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     174    1077    1173    1832    3953    6209    7917 
   Size:        174     903      96     659    2121    2256    1708

  Fragments arranged by size: 

                2256    2121    1708     903     659     174      96
 
BtsI GCAGTG_nn'

Cuts at:      0    1539    2762    3246    5579    5819    7917 
   Size:       1539    1223     484    2333     240    2098

  Fragments arranged by size: 

                2333    2098    1539    1223     484     240
 
ClaI AT'CG_AT

Cuts at:      0    4709    7067    7917 
   Size:       4709    2358     850

  Fragments arranged by size: 

                4709    2358     850
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1191    1226    2085    2120    4848    4883    7917 
   Size:       1191      35     859      35    2728      35    3034

  Fragments arranged by size: 

                3034    2728    1191     859      35      35      35
 
DraIII CAC_nnn'GTG

Cuts at:      0    1135    1365    1718    3990    5039    6036    7917 
   Size:       1135     230     353    2272    1049     997    1881

  Fragments arranged by size: 

                2272    1881    1135    1049     997     353     230
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     780    4341    6892    7917 
   Size:        780    3561    2551    1025

  Fragments arranged by size: 

                3561    2551    1025     780
 
EaeI y'GGCC_r

Cuts at:      0      86     582     933     968    1083    1422    1458    1906 
   Size:         86     496     351      35     115     339      36     448

Cuts at:   1906    2060    2397    3611    3708    3890    4287    5274    5571 
   Size:        154     337    1214      97     182     397     987     297

Cuts at:   5571    5675    5927    5988    6054    6065    6434    6788    7658 
   Size:        104     252      61      66      11     369     354     870

Cuts at:   7658    7917 
   Size:        259

  Fragments arranged by size: 

                1214     987     870     496     448     397     369     354
                 351     339     337     297     259     252     182     154
                 115     104      97      86      66      61      36      35
                  11
 
EagI C'GGCC_G

Cuts at:      0     582     968    3611    7917 
   Size:        582     386    2643    4306

  Fragments arranged by size: 

                4306    2643     582     386
 
EarI CTCTTCn'nnn_

Cuts at:      0    3421    4577    7693    7917 
   Size:       3421    1156    3116     224

  Fragments arranged by size: 

                3421    3116    1156     224
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     121     286     709    1951    7917 
   Size:        121     165     423    1242    5966

  Fragments arranged by size: 

                5966    1242     423     165     121
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     190     421     546     826    1192    1321    1453    1564 
   Size:        190     231     125     280     366     129     132     111

Cuts at:   1564    1777    2259    2456    3529    5992    6286    6413    6739 
   Size:        213     482     197    1073    2463     294     127     326

Cuts at:   6739    6749    6755    6800    7120    7917 
   Size:         10       6      45     320     797

  Fragments arranged by size: 

                2463    1073     797     482     366     326     320     294
                 280     231     213     197     190     132     129     127
                 125     111      45      10       6
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     106    7834    7917 
   Size:        106    7728      83

  Fragments arranged by size: 

                7728     106      83
 
EcoO109I rG'GnC_Cy

Cuts at:      0      99     588     963    1162    1263    1464    1560    1561 
   Size:         99     489     375     199     101     201      96       1

Cuts at:   1561    1593    1746    1747    2112    2446    2634    2635    3028 
   Size:         32     153       1     365     334     188       1     393

Cuts at:   3028    3115    3116    3315    3316    3499    3680    4179    4180 
   Size:         87       1     199       1     183     181     499       1

Cuts at:   4180    4317    4465    4549    4550    4731    5289    5300    5342 
   Size:        137     148      84       1     181     558      11      42

Cuts at:   5342    5664    5685    6141    6231    6232    6261    6667    6668 
   Size:        322      21     456      90       1      29     406       1

Cuts at:   6668    6708    6709    6804    6805    7257    7528    7619    7620 
   Size:         40       1      95       1     452     271      91       1

Cuts at:   7620    7627    7665    7769    7917 
   Size:          7      38     104     148

  Fragments arranged by size: 

                 558     499     489     456     452     406     393     375
                 365     334     322     271     201     199     199     188
                 183     181     181     153     148     148     137     104
                 101      99      96      95      91      90      87      84
                  42      40      38      32      29      21      11       7
                   1       1       1       1       1       1       1       1
                   1       1       1       1
 
EcoRI G'AATT_C

Cuts at:      0    6748    7917 
   Size:       6748    1169
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1122    7722    7917 
   Size:       1122    6600     195

  Fragments arranged by size: 

                6600    1122     195
 
HaeII r_GCGC'y

Cuts at:      0     331     599    1529    3284    7917 
   Size:        331     268     930    1755    4633

  Fragments arranged by size: 

                4633    1755     930     331     268
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     304     336     625     657     748     780    1041    1073 
   Size:        304      32     289      32      91      32     261      32

Cuts at:   1073    4740    4772    5197    5229    5674    5706    7366    7398 
   Size:       3667      32     425      32     445      32    1660      32

Cuts at:   7398    7552    7584    7917 
   Size:        154      32     333

  Fragments arranged by size: 

                3667    1660     445     425     333     304     289     261
                 154      91      32      32      32      32      32      32
                  32      32      32
 
HincII GTy'rAC

Cuts at:      0      63    2067    5565    5640    7017    7917 
   Size:         63    2004    3498      75    1377     900

  Fragments arranged by size: 

                3498    2004    1377     900      75      63
 
KasI G'GCGC_C

Cuts at:      0     595    3280    7917 
   Size:        595    2685    4637

  Fragments arranged by size: 

                4637    2685     595
 
KpnI G_GTAC'C

Cuts at:      0    4591    7917 
   Size:       4591    3326
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     221    6080    7917 
   Size:        221    5859    1837

  Fragments arranged by size: 

                5859    1837     221
 
MscI TGG'CCA

Cuts at:      0     935    1085    1424    2399    3892    4289    5573    5677 
   Size:        935     150     339     975    1493     397    1284     104

Cuts at:   5677    5990    6056    6067    6436    6790    7660    7917 
   Size:        313      66      11     369     354     870     257

  Fragments arranged by size: 

                1493    1284     975     935     870     397     369     354
                 339     313     257     150     104      66      11
 
MslI CAynn'nnrTG

Cuts at:      0     451     457    2233    2542    3638    4675    5143    5851 
   Size:        451       6    1776     309    1096    1037     468     708

Cuts at:   5851    6082    7858    7917 
   Size:        231    1776      59

  Fragments arranged by size: 

                1776    1776    1096    1037     708     468     451     309
                 231      59       6
 
MspA1I CmG'CkG

Cuts at:      0     285     546     611    1299    1328    1501    2195    3043 
   Size:        285     261      65     688      29     173     694     848

Cuts at:   3043    3523    4226    5244    5708    6589    7318    7917 
   Size:        480     703    1018     464     881     729     599

  Fragments arranged by size: 

                1018     881     848     729     703     694     688     599
                 480     464     285     261     173      65      29
 
NaeI GCC'GGC

Cuts at:      0      86    1383    3285    7810    7917 
   Size:         86    1297    1902    4525     107

  Fragments arranged by size: 

                4525    1902    1297     107      86
 
NarI GG'CG_CC

Cuts at:      0     596    3281    7917 
   Size:        596    2685    4636

  Fragments arranged by size: 

                4636    2685     596
 
NcoI C'CATG_G

Cuts at:      0    1268    1346    1631    2234    5604    7442    7917 
   Size:       1268      78     285     603    3370    1838     475

  Fragments arranged by size: 

                3370    1838    1268     603     475     285      78
 
NgoMIV G'CCGG_C

Cuts at:      0      84    1381    3283    7808    7917 
   Size:         84    1297    1902    4525     109

  Fragments arranged by size: 

                4525    1902    1297     109      84
 
NspI r_CATG'y

Cuts at:      0    3365    3948    5523    5735    6043    7743    7917 
   Size:       3365     583    1575     212     308    1700     174

  Fragments arranged by size: 

                3365    1700    1575     583     308     212     174
 
PasI CC'CwG_GG

Cuts at:      0     591    1750    5268    7917 
   Size:        591    1159    3518    2649

  Fragments arranged by size: 

                3518    2649    1159     591
 
PciI A'CATG_T

Cuts at:      0    3361    7917 
   Size:       3361    4556
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1664    6063    7917 
   Size:       1664    4399    1854

  Fragments arranged by size: 

                4399    1854    1664
 
PfoI T'CCnGG_A

Cuts at:      0     338    1259    2132    3626    6488    6872    7917 
   Size:        338     921     873    1494    2862     384    1045

  Fragments arranged by size: 

                2862    1494    1045     921     873     384     338
 
PmlI CAC'GTG

Cuts at:      0    1664    1938    2032    3459    5166    5994    6063    6348 
   Size:       1664     274      94    1427    1707     828      69     285

Cuts at:   6348    6636    7800    7917 
   Size:        288    1164     117

  Fragments arranged by size: 

                1707    1664    1427    1164     828     288     285     274
                 117      94      69
 
PpuMI rG'GwC_Cy

Cuts at:      0      99     963    1162    1263    1593    4317    6261    7257 
   Size:         99     864     199     101     330    2724    1944     996

Cuts at:   7257    7627    7769    7917 
   Size:        370     142     148

  Fragments arranged by size: 

                2724    1944     996     864     370     330     199     148
                 142     101      99
 
PshAI GACnn'nnGTC

Cuts at:      0    4010    7917 
   Size:       4010    3907
 
PspOMI G'GGCC_C

Cuts at:      0     587    1560    1746    2634    3027    3115    3315    4179 
   Size:        587     973     186     888     393      88     200     864

Cuts at:   4179    4363    4465    4549    5288    5685    6231    6667    6708 
   Size:        184     102      84     739     397     546     436      41

Cuts at:   6708    6804    7619    7917 
   Size:         96     815     298

  Fragments arranged by size: 

                 973     888     864     815     739     587     546     436
                 397     393     298     200     186     184     102      96
                  88      84      41
 
PspXI vC'TCGA_Gb

Cuts at:      0     331    3334    3555    7917 
   Size:        331    3003     221    4362

  Fragments arranged by size: 

                4362    3003     331     221
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    6550    6582    7917 
   Size:       6550      32    1335

  Fragments arranged by size: 

                6550    1335      32
 
PstI C_TGCA'G

Cuts at:      0     203    1333    3233    3743    6174    7723    7917 
   Size:        203    1130    1900     510    2431    1549     194

  Fragments arranged by size: 

                2431    1900    1549    1130     510     203     194
 
PvuI CG_AT'CG

Cuts at:      0    7067    7917 
   Size:       7067     850
 
PvuII CAG'CTG

Cuts at:      0     546     611    1299    1501    2195    4226    5244    5708 
   Size:        546      65     688     202     694    2031    1018     464

Cuts at:   5708    7318    7917 
   Size:       1610     599

  Fragments arranged by size: 

                2031    1610    1018     694     688     599     546     464
                 202      65
 
SalI G'TCGA_C

Cuts at:      0      61    7917 
   Size:         61    7856
 
SanDI GG'GwC_CC

Cuts at:      0    7257    7917 
   Size:       7257     660
 
SapI GCTCTTCn'nnn_

Cuts at:      0    7693    7917 
   Size:       7693     224
 
ScaI AGT'ACT

Cuts at:      0    4568    7917 
   Size:       4568    3349
 
SexAI A'CCwGG_T

Cuts at:      0    6032    7917 
   Size:       6032    1885
 
SfcI C'TryA_G

Cuts at:      0     199    1329    3229    3739    4172    4263    4554    5769 
   Size:        199    1130    1900     510     433      91     291    1215

Cuts at:   5769    6170    7719    7815    7917 
   Size:        401    1549      96     102

  Fragments arranged by size: 

                1900    1549    1215    1130     510     433     401     291
                 199     102      96      91
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    3889    7917 
   Size:       3889    4028
 
SfoI GGC'GCC

Cuts at:      0     597    3282    7917 
   Size:        597    2685    4635

  Fragments arranged by size: 

                4635    2685     597
 
SmaI CCC'GGG

Cuts at:      0     620    1309    2521    3393    3504    3678    3847    4069 
   Size:        620     689    1212     872     111     174     169     222

Cuts at:   4069    4659    6766    7625    7917 
   Size:        590    2107     859     292

  Fragments arranged by size: 

                2107    1212     872     859     689     620     590     292
                 222     174     169     111
 
SmlI C'TyrA_G

Cuts at:      0     310     331    2350    3334    3555    7834    7917 
   Size:        310      21    2019     984     221    4279      83

  Fragments arranged by size: 

                4279    2019     984     310     221      83      21
 
SnaBI TAC'GTA

Cuts at:      0    7689    7917 
   Size:       7689     228
 
SrfI GCCC'GGGC

Cuts at:      0    3847    7917 
   Size:       3847    4070
 
StuI AGG'CCT

Cuts at:      0    3068    4475    6785    7684    7917 
   Size:       3068    1407    2310     899     233

  Fragments arranged by size: 

                3068    2310    1407     899     233
 
StyI C'CwwG_G

Cuts at:      0     794    1158    1268    1346    1475    1565    1631    2234 
   Size:        794     364     110      78     129      90      66     603

Cuts at:   2234    2744    3275    4346    5604    5645    6014    6672    7055 
   Size:        510     531    1071    1258      41     369     658     383

Cuts at:   7055    7073    7385    7442    7592    7661    7765    7917 
   Size:         18     312      57     150      69     104     152

  Fragments arranged by size: 

                1258    1071     794     658     603     531     510     383
                 369     364     312     152     150     129     110     104
                  90      78      69      66      57      41      18
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    2027    2692    3442    4063    4221    4531    5299    5490 
   Size:       2027     665     750     621     158     310     768     191

Cuts at:   5490    5617    7216    7917 
   Size:        127    1599     701

  Fragments arranged by size: 

                2027    1599     768     750     701     665     621     310
                 191     158     127
 
TatI w'GTAC_w

Cuts at:      0    1514    2510    2807    3620    3947    3959    4238    4566 
   Size:       1514     996     297     813     327      12     279     328

Cuts at:   4566    5255    5786    6620    7850    7917 
   Size:        689     531     834    1230      67

  Fragments arranged by size: 

                1514    1230     996     834     813     689     531     328
                 327     297     279      67      12
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     103     135    2108    2140    2930    2962    6262    6294 
   Size:        103      32    1973      32     790      32    3300      32

Cuts at:   6294    7917 
   Size:       1623

  Fragments arranged by size: 

                3300    1973    1623     790     103      32      32      32
                  32
 
Tth111I GACn'n_nGTC

Cuts at:      0    5353    7917 
   Size:       5353    2564
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     885    4476    4692    6103    6349    6637    7392    7653 
   Size:        885    3591     216    1411     246     288     755     261

Cuts at:   7653    7917 
   Size:        264

  Fragments arranged by size: 

                3591    1411     885     755     288     264     261     246
                 216
 
XhoI C'TCGA_G

Cuts at:      0     331    3334    3555    7917 
   Size:        331    3003     221    4362

  Fragments arranged by size: 

                4362    3003     331     221
 
XmaI C'CCGG_G

Cuts at:      0     618    1307    2519    3391    3502    3676    3845    4067 
   Size:        618     689    1212     872     111     174     169     222

Cuts at:   4067    4657    6764    7623    7917 
   Size:        590    2107     859     294

  Fragments arranged by size: 

                2107    1212     872     859     689     618     590     294
                 222     174     169     111
 
XmnI GAAnn'nnTTC

Cuts at:      0     142    6920    7917 
   Size:        142    6778     997

  Fragments arranged by size: 

                6778     997     142
 
ZraI GAC'GTC

Cuts at:      0    2409    2724    7185    7917 
   Size:       2409     315    4461     732

  Fragments arranged by size: 

                4461    2409     732     315
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI   AflIII     AleI
     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AvaI    AvrII
    BamHI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI     BclI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI    HaeII    Hin4I
   HincII     KasI     KpnI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI   NgoMIV     NspI     PasI     PciI    PflMI     PfoI
     PmlI    PpuMI    PshAI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII     SalI    SanDI     SapI     ScaI    SexAI     SfcI     SfiI
     SfoI     SmaI     SmlI    SnaBI     SrfI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AfeI    AflII     AgeI     AhdI     AscI     AseI    AsiSI     BaeI
    BciVI    BfrBI     BmtI    BsiWI    BspEI   BssHII     DraI  EcoICRI
    EcoRV     FseI     FspI    FspAI  HindIII     HpaI     MfeI     MluI
     NdeI     NheI     NotI     NruI     NsiI     PacI     PmeI     PpiI
     PsiI    RsrII     SacI    SacII     SbfI     SgfI    SgrAI     SpeI
     SphI     SspI     SwaI     XbaI
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