Restriction map for pF1KF0267
 (Linear) (Six-base) MAPSORT of: FLJ00267.seq  Check: 1391  from: 1  to: 3303



FLJ00267.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:13  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      83     764    2889    3303 
   Size:         83     681    2125     414

  Fragments arranged by size: 

                2125     681     414      83
 
AccI GT'mk_AC

Cuts at:      0    2224    3303 
   Size:       2224    1079
 
Acc65I G'GTAC_C

Cuts at:      0    2417    3303 
   Size:       2417     886
 
AclI AA'CG_TT

Cuts at:      0    1310    3303 
   Size:       1310    1993
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1814    2501    2606    3182    3303 
   Size:       1814     687     105     576     121

  Fragments arranged by size: 

                1814     687     576     121     105
 
AfeI AGC'GCT

Cuts at:      0     376    3303 
   Size:        376    2927
 
AflIII A'CryG_T

Cuts at:      0     627    3303 
   Size:        627    2676
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1503    3303 
   Size:       1503    1800
 
AleI CACnn'nnGTG

Cuts at:      0     628    3303 
   Size:        628    2675
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2907    3303 
   Size:       2907     396
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     398     430    3303 
   Size:        398      32    2873

  Fragments arranged by size: 

                2873     398      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1561    1763    1932    2479    3303 
   Size:       1561     202     169     547     824

  Fragments arranged by size: 

                1561     824     547     202     169
 
ApaI G_GGCC'C

Cuts at:      0    1040    2319    3303 
   Size:       1040    1279     984

  Fragments arranged by size: 

                1279    1040     984
 
ApoI r'AATT_y

Cuts at:      0     124     205    3303 
   Size:        124      81    3098

  Fragments arranged by size: 

                3098     124      81
 
AvaI C'yCGr_G

Cuts at:      0     704     721    2895    2945    3011    3303 
   Size:        704      17    2174      50      66     292

  Fragments arranged by size: 

                2174     704     292      66      50      17
 
BamHI G'GATC_C

Cuts at:      0     306    3181    3303 
   Size:        306    2875     122

  Fragments arranged by size: 

                2875     306     122
 
BanI G'GyrC_C

Cuts at:      0      96    2417    2817    2877    3303 
   Size:         96    2321     400      60     426

  Fragments arranged by size: 

                2321     426     400      96      60
 
BanII G_rGCy'C

Cuts at:      0     522    1040    1667    2319    3155    3303 
   Size:        522     518     627     652     836     148

  Fragments arranged by size: 

                 836     652     627     522     518     148
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    2459    3303 
   Size:       2459     844
 
BbvCI CC'TCA_GC

Cuts at:      0     344     532     929    2336    2372    2534    2678    3303 
   Size:        344     188     397    1407      36     162     144     625

  Fragments arranged by size: 

                1407     625     397     344     188     162     144      36
            
 
BfrBI ATG'CAT

Cuts at:      0     492    3262    3303 
   Size:        492    2770      41

  Fragments arranged by size: 

                2770     492      41
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1026    1189    2703    2753    2907    3303 
   Size:       1026     163    1514      50     154     396

  Fragments arranged by size: 

                1514    1026     396     163     154      50
 
BlpI GC'TnA_GC

Cuts at:      0    1988    3303 
   Size:       1988    1315
 
Bme1580I G_kGCm'C

Cuts at:      0     101     480    1040    1292    1348    2319    2822    3303 
   Size:        101     379     560     252      56     971     503     481

  Fragments arranged by size: 

                 971     560     503     481     379     252     101      56
            
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1144    2705    2843    3303 
   Size:       1144    1561     138     460

  Fragments arranged by size: 

                1561    1144     460     138
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1713    3303 
   Size:       1713    1590
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     454    1513    1678    1918    2015    2573    2629    2945 
   Size:        454    1059     165     240      97     558      56     316

Cuts at:   2945    3169    3303 
   Size:        224     134

  Fragments arranged by size: 

                1059     558     454     316     240     224     165     134
                  97      56
 
Bpu10I CC'TnA_GC

Cuts at:      0     344     371     532     929    2336    2372    2534    2678 
   Size:        344      27     161     397    1407      36     162     144

Cuts at:   2678    3139    3303 
   Size:        461     164

  Fragments arranged by size: 

                1407     461     397     344     164     162     161     144
                  36      27
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1360    1431    1530    3020    3303 
   Size:       1360      71      99    1490     283

  Fragments arranged by size: 

                1490    1360     283      99      71
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     180    2032    2534    3303 
   Size:        180    1852     502     769

  Fragments arranged by size: 

                1852     769     502     180
 
BsaAI yAC'GTr

Cuts at:      0     630    3303 
   Size:        630    2673
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     253     283     667     697    1513    1543    2401    2431 
   Size:        253      30     384      30     816      30     858      30

Cuts at:   2431    3303 
   Size:        872

  Fragments arranged by size: 

                 872     858     816     384     253      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     148     168     261    1675    1705    1816    2228    2353 
   Size:        148      20      93    1414      30     111     412     125

Cuts at:   2353    2501    2701    3119    3303 
   Size:        148     200     418     184

  Fragments arranged by size: 

                1414     418     412     200     184     148     148     125
                 111      93      30      20
 
BseYI C'CCAG_C

Cuts at:      0    1003    1039    1171    1192    1756    1961    2083    3303 
   Size:       1003      36     132      21     564     205     122    1220

  Fragments arranged by size: 

                1220    1003     564     205     132     122      36      21
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     140    1229    1537    3188    3303 
   Size:        140    1089     308    1651     115

  Fragments arranged by size: 

                1651    1089     308     140     115
 
BsiHKAI G_wGCw'C

Cuts at:      0      74     833    1667    3155    3303 
   Size:         74     759     834    1488     148

  Fragments arranged by size: 

                1488     834     759     148      74
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     273    3303 
   Size:        273    3030
 
Bsp1286I G_dGCh'C

Cuts at:      0      74     101     480     522     833    1040    1292    1348 
   Size:         74      27     379      42     311     207     252      56

Cuts at:   1348    1667    2319    2822    3155    3303 
   Size:        319     652     503     333     148

  Fragments arranged by size: 

                 652     503     379     333     319     311     252     207
                 148      74      56      42      27
 
BspHI T'CATG_A

Cuts at:      0     443    3303 
   Size:        443    2860
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      83     239     536     764    2889    3242    3303 
   Size:         83     156     297     228    2125     353      61

  Fragments arranged by size: 

                2125     353     297     228     156      83      61
 
BsrBI CCG'CTC

Cuts at:      0      45     219    2804    3303 
   Size:         45     174    2585     499

  Fragments arranged by size: 

                2585     499     174      45
 
BsrDI GCAATG_nn'

Cuts at:      0    1060    2861    3303 
   Size:       1060    1801     442

  Fragments arranged by size: 

                1801    1060     442
 
BsrFI r'CCGG_y

Cuts at:      0     323    1361    2906    3303 
   Size:        323    1038    1545     397

  Fragments arranged by size: 

                1545    1038     397     323
 
BsrGI T'GTAC_A

Cuts at:      0    2355    3303 
   Size:       2355     948
 
BssSI C'ACGA_G

Cuts at:      0    1166    3303 
   Size:       1166    2137
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     609     643     981    2446    2551    3303 
   Size:        609      34     338    1465     105     752

  Fragments arranged by size: 

                1465     752     609     338     105      34
 
BstYI r'GATC_y

Cuts at:      0     155     306     386    3181    3303 
   Size:        155     151      80    2795     122

  Fragments arranged by size: 

                2795     155     151     122      80
 
BstZ17I GTA'TAC

Cuts at:      0    2225    3303 
   Size:       2225    1078
 
Bsu36I CC'TnA_GG

Cuts at:      0     555    1121    1682    2699    3303 
   Size:        555     566     561    1017     604

  Fragments arranged by size: 

                1017     604     566     561     555
 
BtgI C'CryG_G

Cuts at:      0     919    1332    1769    3303 
   Size:        919     413     437    1534

  Fragments arranged by size: 

                1534     919     437     413
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     794    3303 
   Size:        794    2509
 
BtsI GCAGTG_nn'

Cuts at:      0     261     742     961    3303 
   Size:        261     481     219    2342

  Fragments arranged by size: 

                2342     481     261     219
 
DraIII CAC_nnn'GTG

Cuts at:      0     994    1138    3303 
   Size:        994     144    2165

  Fragments arranged by size: 

                2165     994     144
 
EaeI y'GGCC_r

Cuts at:      0     506     894     967    1051    1319    1363    2904    3303 
   Size:        506     388      73      84     268      44    1541     399

  Fragments arranged by size: 

                1541     506     399     388     268      84      73      44
            
 
EarI CTCTTCn'nnn_

Cuts at:      0    1943    2012    2126    2144    2252    2564    2649    2651 
   Size:       1943      69     114      18     108     312      85       2

Cuts at:   2651    3303 
   Size:        652

  Fragments arranged by size: 

                1943     652     312     114     108      85      69      18
                   2
 
EcoICRI GAG'CTC

Cuts at:      0    1665    3153    3303 
   Size:       1665    1488     150

  Fragments arranged by size: 

                1665    1488     150
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     454    1513    1678    1814    1918    2015    2501    2573 
   Size:        454    1059     165     136     104      97     486      72

Cuts at:   2573    2606    2629    2945    3169    3182    3303 
   Size:         33      23     316     224      13     121

  Fragments arranged by size: 

                1059     486     454     316     224     165     136     121
                 104      97      72      33      23      13
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     280    1320    2964    3287    3303 
   Size:        280    1040    1644     323      16

  Fragments arranged by size: 

                1644    1040     323     280      16
 
EcoO109I rG'GnC_Cy

Cuts at:      0     428     701     726    1036    1861    2301    2315    2942 
   Size:        428     273      25     310     825     440      14     627

Cuts at:   2942    3303 
   Size:        361

  Fragments arranged by size: 

                 825     627     440     428     361     310     273      25
                  14
 
HaeII r_GCGC'y

Cuts at:      0     378    3303 
   Size:        378    2925
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     931     963    1489    1513    1521    1545    1708    1740 
   Size:        931      32     526      24       8      24     163      32

Cuts at:   1740    2566    2598    3018    3050    3142    3174    3303 
   Size:        826      32     420      32      92      32     129

  Fragments arranged by size: 

                 931     826     526     420     163     129      92      32
                  32      32      32      32      24      24       8
 
HincII GTy'rAC

Cuts at:      0    1254    2616    3303 
   Size:       1254    1362     687

  Fragments arranged by size: 

                1362    1254     687
 
HindIII A'AGCT_T

Cuts at:      0     193    3303 
   Size:        193    3110
 
KpnI G_GTAC'C

Cuts at:      0    2421    3303 
   Size:       2421     882
 
MfeI C'AATT_G

Cuts at:      0     361    3303 
   Size:        361    2942
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     206     974    1409    2817    3303 
   Size:        206     768     435    1408     486

  Fragments arranged by size: 

                1408     768     486     435     206
 
MscI TGG'CCA

Cuts at:      0     508     896    1321    3303 
   Size:        508     388     425    1982

  Fragments arranged by size: 

                1982     508     425     388
 
MslI CAynn'nnrTG

Cuts at:      0     586     607     628    3303 
   Size:        586      21      21    2675

  Fragments arranged by size: 

                2675     586      21      21
 
MspA1I CmG'CkG

Cuts at:      0     574    1003    1760    1780    3303 
   Size:        574     429     757      20    1523

  Fragments arranged by size: 

                1523     757     574     429      20
 
NaeI GCC'GGC

Cuts at:      0    2908    3303 
   Size:       2908     395
 
NdeI CA'TA_TG

Cuts at:      0    3258    3303 
   Size:       3258      45
 
NgoMIV G'CCGG_C

Cuts at:      0    2906    3303 
   Size:       2906     397
 
NsiI A_TGCA'T

Cuts at:      0     494    3264    3303 
   Size:        494    2770      39

  Fragments arranged by size: 

                2770     494      39
 
NspI r_CATG'y

Cuts at:      0     492     496    1961    2766    3303 
   Size:        492       4    1465     805     537

  Fragments arranged by size: 

                1465     805     537     492       4
 
PasI CC'CwG_GG

Cuts at:      0    1627    1642    3283    3303 
   Size:       1627      15    1641      20

  Fragments arranged by size: 

                1641    1627      20      15
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     515    1171    3303 
   Size:        515     656    2132

  Fragments arranged by size: 

                2132     656     515
 
PfoI T'CCnGG_A

Cuts at:      0    2922    3184    3303 
   Size:       2922     262     119

  Fragments arranged by size: 

                2922     262     119
 
PmlI CAC'GTG

Cuts at:      0     630    3303 
   Size:        630    2673
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1288    1320    3303 
   Size:       1288      32    1983

  Fragments arranged by size: 

                1983    1288      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     428     701     726    1861    2301    2942    3303 
   Size:        428     273      25    1135     440     641     361

  Fragments arranged by size: 

                1135     641     440     428     361     273      25
 
PshAI GACnn'nnGTC

Cuts at:      0     233    1847    2104    2723    3303 
   Size:        233    1614     257     619     580

  Fragments arranged by size: 

                1614     619     580     257     233
 
PspOMI G'GGCC_C

Cuts at:      0    1036    2315    3303 
   Size:       1036    1279     988

  Fragments arranged by size: 

                1279    1036     988
 
PstI C_TGCA'G

Cuts at:      0      80     773    1002    3303 
   Size:         80     693     229    2301

  Fragments arranged by size: 

                2301     693     229      80
 
PvuII CAG'CTG

Cuts at:      0    1003    1760    3303 
   Size:       1003     757    1543

  Fragments arranged by size: 

                1543    1003     757
 
SacI G_AGCT'C

Cuts at:      0    1667    3155    3303 
   Size:       1667    1488     148

  Fragments arranged by size: 

                1667    1488     148
 
SanDI GG'GwC_CC

Cuts at:      0     701    2942    3303 
   Size:        701    2241     361

  Fragments arranged by size: 

                2241     701     361
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1943    3303 
   Size:       1943    1360
 
SfcI C'TryA_G

Cuts at:      0      76     350     423     769     998    1091    1354    3303 
   Size:         76     274      73     346     229      93     263    1949

  Fragments arranged by size: 

                1949     346     274     263     229      93      76      73
            
 
SmaI CCC'GGG

Cuts at:      0     706    3013    3303 
   Size:        706    2307     290

  Fragments arranged by size: 

                2307     706     290
 
SmlI C'TyrA_G

Cuts at:      0    1339    1446    1545    3035    3303 
   Size:       1339     107      99    1490     268

  Fragments arranged by size: 

                1490    1339     268     107      99
 
SphI G_CATG'C

Cuts at:      0     496    1961    2766    3303 
   Size:        496    1465     805     537

  Fragments arranged by size: 

                1465     805     537     496
 
StuI AGG'CCT

Cuts at:      0     286     553    1791    2901    3303 
   Size:        286     267    1238    1110     402

  Fragments arranged by size: 

                1238    1110     402     286     267
 
StyI C'CwwG_G

Cuts at:      0    1316    1401    2042    2144    2439    3303 
   Size:       1316      85     641     102     295     864

  Fragments arranged by size: 

                1316     864     641     295     102      85
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      44     671    1126    1557    2740    3303 
   Size:         44     627     455     431    1183     563

  Fragments arranged by size: 

                1183     627     563     455     431      44
 
TatI w'GTAC_w

Cuts at:      0    2355    3061    3303 
   Size:       2355     706     242

  Fragments arranged by size: 

                2355     706     242
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     253     285     667     699    3303 
   Size:        253      32     382      32    2604

  Fragments arranged by size: 

                2604     382     253      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2290    3303 
   Size:       2290    1013
 
XmaI C'CCGG_G

Cuts at:      0     704    3011    3303 
   Size:        704    2307     292

  Fragments arranged by size: 

                2307     704     292
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI     AfeI   AflIII     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI     ApoI     AvaI    BamHI
     BanI    BanII     BbsI    BbvCI    BfrBI     BglI     BlpI Bme1580I
     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaXI
    BseRI    BseYI     BsgI  BsiHKAI    BsmBI Bsp1286I    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI    BssSI    BstXI    BstYI  BstZ17I
   Bsu36I     BtgI    BtgZI     BtsI   DraIII     EaeI     EarI  EcoICRI
  Eco57MI    EcoNI EcoO109I    HaeII    Hin4I   HincII  HindIII     KpnI
     MfeI     MmeI     MscI     MslI   MspA1I     NaeI     NdeI   NgoMIV
     NsiI     NspI     PasI    PflMI     PfoI     PmlI     PpiI    PpuMI
    PshAI   PspOMI     PstI    PvuII     SacI    SanDI     SapI     SfcI
     SmaI     SmlI     SphI     StuI     StyI    TaqII     TatI     TstI
     XcmI     XmaI

 Enzymes that do not cut: 

    AatII    AflII     AgeI    ApaLI     AscI     AseI    AsiSI    AvrII
     BaeI     BbeI     BcgI    BciVI     BclI    BglII    BmgBI     BmtI
    BsaBI    BsaHI    BsaWI    BsiEI    BsiWI     BsmI    BspEI   BssHII
   BstAPI    BstBI   BstEII     ClaI    CspCI     DraI     DrdI     EagI
     EciI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI     HpaI
     KasI     MluI     NarI     NcoI     NheI     NotI     NruI     PacI
     PciI     PmeI     PsiI    PspXI     PsrI     PvuI    RsrII    SacII
     SalI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
    SnaBI     SpeI     SrfI     SspI     SwaI  Tth111I     XbaI     XhoI
     XmnI     ZraI
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