Restriction map for pF1KB9891
##################################
# NO CUT ENZYMES (  152 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AgeI        AhdI        
AocI        Aor51HI     ApaI        AscI        AseI        
AsnI        Asp718I     AspEI       AspI        AvaI        
AviII       AvrII       BalI        BbeI        BbrPI       
BbsI        BbvCI       BcgI        BclI        BcnI        
BfrI        BglI        BlnI        BlpI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsaHI       BsaWI       
BseAI       BsiEI       BsiWI       BsmI        BsoBI       
Bsp1407I    BspDI       BspEI       BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstXI       BstZ17I     Bsu36I      CelII       Cfr10I      
ClaI        CpoI        DraI        DraII       DraIII      
DrdI        EaeI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoRV       EcoT14I     FauI        
FbaI        FseI        FspI        HapII       HgaI        
Hin1I       HindIII     HpaII       KasI        KpnI        
KspI        MaeII       MamI        MluI        MluNI       
MroI        MscI        MspI        MvnI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NspV        PacI        
PaeR7I      PflFI       PinAI       PleI        PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        RsrII       SacI        
SacII       SalI        SbfI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StyI        
SwaI        Tth111I     XbaI        XcmI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AflIII        372  ACATGT               A^C[GA][CT]GT
Alw44I       1233  GTGCAC               G^TGCAC
AlwNI         806  CAGAACCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaLI        1233  GTGCAC               G^TGCAC
Asp700I      1146  GAAGATCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI        1233  GTGCAC               G[AT]GC[AT]^C
AvaII        1330  GGACC                G^G[AT]CC
BamHI        1369  GGATCC               G^GATCC
BanI         1103  GGCACC               G^G[CT][GA]CC
BanII        1074  GAGCCC               G[GA]GC[CT]^C
BbvI         1171  GCTGC                GCAGC(8/12)
BfaI         1226  CTAG                 C^TAG
BglII        1148  AGATCT               A^GATCT
BsaI          326  GGTCTC               GGTCTC(1/5)
BsaJI         968  CCACGG               C^C[ACGT][ACGT]GG
BsgI         1069  GTGCAG               GTGCAG(16/14)
BsiHKAI      1233  GTGCAC               G[AT]GC[AT]^C
BsiYI         661  CCACCTCCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          661  CCACCTCCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI         340  CGTCTC               CGTCTC(1/5)
BsmFI         255  GGGAC                GGGAC(10/14)
BspHI         420  TCATGA               T^CATGA
BspLU11I      372  ACATGT               A^CATGT
BspMI         810  ACCTGC               ACCTGC(4/8)
Cfr13I       1330  GGACC                G^G[ACGT]CC
DsaI          968  CCACGG               C^C[GA][CT]GG
EcoT22I       872  ATGCAT               ATGCA^T
HaeII         395  GGCGCT               [GA]GCGC^[CT]
HincII        955  GTTAAC               GT[CT]^[GA]AC
HindII        955  GTTAAC               GT[CT]^[GA]AC
HinfI         711  GAATC                G^A[ACGT]TC
HpaI          955  GTTAAC               GTT^AAC
MaeI         1226  CTAG                 C^TAG
MspA1I         62  CAGCTG               C[AC]G^C[GT]G
NsiI          872  ATGCAT               ATGCA^T
PflMI         661  CCACCTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII          62  CAGCTG               CAG^CTG
RcaI          420  TCATGA               T^CATGA
SapI         1174  GCTCTTC              GCTCTTC(1/4)
Sau96I       1330  GGACC                G^G[ACGT]CC
ScaI         1297  AGTACT               AGT^ACT
SexAI         322  ACCAGGT              A^CC[AT]GGT
SfaNI         874  GCATC                GCATC(5/9)
StuI          297  AGGCCT               AGG^CCT
TfiI          711  GAATC                G^A[AT]TC
TseI         1171  GCTGC                G^C[AT]GC
Tsp45I        698  GTGAC                ^GT[GC]AC
Van91I        661  CCACCTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI         1146  GAAGATCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AfaI          263  GTAC                 GT^AC
             1298  GTAC                
BciVI         288  GGATAC               GTATCC(6/5)
              349  GTATCC              
CfoI          396  GCGC                 GCG^C
             1008  GCGC                
Fnu4HI         24  GCCGC                GC^[ACGT]GC
             1171  GCTGC               
HaeIII        298  GGCC                 GG^CC
              401  GGCC                
HhaI          396  GCGC                 GCG^C
             1008  GCGC                
HinP1I        396  GCGC                 G^CGC
             1008  GCGC                
HphI          144  GGTGA                GGTGA(8/7)
              194  TCACC               
ItaI           24  GCCGC                GC^[ACGT]GC
             1171  GCTGC               
MfeI          434  CAATTG               C^AATTG
             1080  CAATTG              
MunI          434  CAATTG               C^AATTG
             1080  CAATTG              
NlaIV        1103  GGCACC               GG[ACGT]^[ACGT]CC
             1369  GGATCC              
NspI          372  ACATGT               [GA]CATG^[CT]
             1394  GCATGT              
RsaI          263  GTAC                 GT^AC
             1298  GTAC                
SfcI          893  CTGTAG               C^T[GA][CT]AG
             1336  CTACAG              
TaqI          357  TCGA                 T^CGA
              826  TCGA                
TthHB8I       357  TCGA                 T^CGA
              826  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   40 enzymes) #
#########################################

AciI        AcsI        AluI        AlwI        ApoI        
BmyI        BpmI        BseRI       BsmAI       Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       BstYI       
Cac8I       DdeI        DpnII       EarI        EcoRII      
FokI        Ksp632I     MaeIII      MboI        MboII       
MflI        MnlI        MseI        MslI        MvaI        
MwoI        NdeII       NlaIII      Sau3AI      ScrFI       
SmlI        Tru9I       Tsp509I     TspRI       XhoII       
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