Restriction map for pF1KB9885
##################################
# NO CUT ENZYMES (  108 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AflIII      AgeI        AhdI        Alw44I      Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp718I     AspEI       AspI        AviII       BamHI       
BbeI        BbrPI       BbsI        BciVI       BclI        
BpuAI       BsaBI       BsaI        BseAI       BsiEI       
BsiWI       BsmBI       BspDI       BspEI       BspHI       
BspLU11I    BsrFI       BssHII      BstAPI      BstBI       
BstEII      BstPI       BstUI       Cfr10I      ClaI        
CpoI        DraI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FseI        FspI        HaeII       
KasI        KpnI        KspI        MamI        MfeI        
MluI        MroI        MunI        MvnI        NaeI        
NarI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacII       SalI        SbfI        SexAI       SfoI        
SfuI        SgrAI       SmaI        SnaBI       SphI        
Sse8387I    SspI        SwaI        Tth111I     XbaI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   34 enzymes) #
###################################

AflII         841  CTTAAG               C^TTAAG
AvaI          809  CTCGGG               C^[CT]CG[GA]G
AvrII        1006  CCTAGG               C^CTAGG
BanI         1573  GGCACC               G^G[CT][GA]CC
BcgI         1677  GCAGCTGGGTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI          841  CTTAAG               C^TTAAG
BlnI         1006  CCTAGG               C^CTAGG
BsaAI        1132  TACGTG               [CT]AC^GT[GA]
BsaWI        1854  TCCGGT               [AT]^CCGG[AT]
BsmI           55  GAATGC               GAATGC(1/-1)
BsoBI         809  CTCGGG               C^[CT]CG[GA]G
Bsp1407I      400  TGTACA               T^GTACA
BsrBI         430  GAGCGG               CCGCTC(-3/-3)
BsrGI         400  TGTACA               T^GTACA
BssSI        1754  CTCGTG               CACGAG(-5/-1)
Bst1107I     1181  GTATAC               GTA^TAC
BstZ17I      1181  GTATAC               GTA^TAC
CfoI          576  GCGC                 GCG^C
DraIII       1378  CACTGCGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         1844  GACTTACCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI           85  GAAGAG               CTCTTC(1/4)
EcoNI        1388  CCTGTCACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HhaI          576  GCGC                 GCG^C
HinP1I        576  GCGC                 G^CGC
Ksp632I        85  GAAGAG               CTCTTC(1/4)
NspI         1739  GCATGT               [GA]CATG^[CT]
PleI           73  GAGTC                GAGTC(4/5)
PshAI        1777  GACAGTAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI           85  GAAGAGC              GCTCTTC(1/4)
ScaI         1152  AGTACT               AGT^ACT
SfiI          921  GGCCATGGAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI         1155  ACTAGT               A^CTAGT
SspBI         400  TGTACA               T^GTACA
StuI         1599  AGGCCT               AGG^CCT


###################################
# TWO CUT ENZYMES (   33 enzymes) #
###################################

AccI         1181  GTATAC               GT^[AC][GT]AC
             1579  GTCTAC              
AcsI         1549  AAATTT               [GA]^AATT[CT]
             1793  AAATTC              
AcyI          314  GGCGTC               G[GA]^CG[CT]C
              434  GGCGTC              
AfaI          401  GTAC                 GT^AC
             1153  GTAC                
AlwI         1501  GGATC                GGATC(4/5)
             1908  GGATC               
ApoI         1549  AAATTT               [GA]^AATT[CT]
             1793  AAATTC              
Asp700I       609  GAAGTTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1943  GAAGATATTC          
BbvCI         424  GCTGAGG              CCTCAGC(-5/-2)
             1567  GCTGAGG             
BglI          922  GCCATGGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1229  GCCCCAGTGGC         
BglII        1272  AGATCT               A^GATCT
             1922  AGATCT              
BsaHI         314  GGCGTC               G[GA]^CG[CT]C
              434  GGCGTC              
BsgI          109  CTGCAC               GTGCAG(16/14)
              697  GTGCAG              
BspMI         677  ACCTGC               ACCTGC(4/8)
             1017  ACCTGC              
BstXI          62  CCATCTGCATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              555  CCAGGAGTATGG        
BstYI        1272  AGATCT               [GA]^GATC[CT]
             1922  AGATCT              
Hin1I         314  GGCGTC               G[GA]^CG[CT]C
              434  GGCGTC              
HincII        456  GTTAAC               GT[CT]^[GA]AC
              901  GTTAAC              
HindII        456  GTTAAC               GT[CT]^[GA]AC
              901  GTTAAC              
HpaI          456  GTTAAC               GTT^AAC
              901  GTTAAC              
MaeII         905  ACGT                 A^CGT
             1133  ACGT                
MflI         1272  AGATCT               [GA]^GATC[CT]
             1922  AGATCT              
MslI           63  CATCTGCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1740  CATGTGTGTG          
NcoI          923  CCATGG               C^CATGG
             1414  CCATGG              
PflMI         135  CCAGTGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1377  CCACTGCGTGG         
PpuMI         524  AGGACCT              [GA]G^G[AT]CC[CT]
             1032  GGGTCCT             
PstI            5  CTGCAG               CTGCA^G
              487  CTGCAG              
RsaI          401  GTAC                 GT^AC
             1153  GTAC                
TaqI         1497  TCGA                 T^CGA
             1836  TCGA                
TfiI          913  GATTC                G^A[AT]TC
             1614  GAATC               
TthHB8I      1497  TCGA                 T^CGA
             1836  TCGA                
Van91I        135  CCAGTGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1377  CCACTGCGTGG         
XhoII        1272  AGATCT               [GA]^GATC[CT]
             1922  AGATCT              
XmnI          609  GAAGTTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1943  GAAGATATTC          


#########################################
# 3 OR MORE CUT ENZYMES (   83 enzymes) #
#########################################

AciI        AluI        AlwNI       AocI        AspHI       
AvaII       BalI        BanII       BbvI        BcnI        
BfaI        BlpI        BmyI        BpmI        Bpu1102I    
BsaJI       BseRI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BsrDI       BsrI        
BssKI       BstF5I      BstNI       Bsu36I      Cac8I       
CelII       Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        Eco81I      EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeIII      
HapII       HgaI        HindIII     HinfI       HpaII       
HphI        ItaI        MaeI        MaeIII      MboI        
MboII       MluNI       MnlI        MscI        MseI        
MspA1I      MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PvuII       SacI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        StyI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       XcmI        
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