Restriction map for
pF1KB9882
(Circular) (Six-base) MAPSORT of: pF1KB9882.seq Check: 9010 from: 1 to: 4952
pF1KB9882 4952 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AccI GT'mk_AC
Cuts at: 1463 1990 1463
Size: 527 4425
Acc65I G'GTAC_C
Cuts at: 1968 1968
Size: 4952
AclI AA'CG_TT
Cuts at: 4792 4792
Size: 4952
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2777 3209 3956 4224 2777
Size: 432 747 268 3505
Fragments arranged by size:
3505 747 432 268
AfeI AGC'GCT
Cuts at: 742 742
Size: 4952
AflII C'TTAA_G
Cuts at: 1711 1711
Size: 4952
AflIII A'CryG_T
Cuts at: 689 2304 3408 4385 689
Size: 1615 1104 977 1256
Fragments arranged by size:
1615 1256 1104 977
AgeI A'CCGG_T
Cuts at: 3340 3340
Size: 4952
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1402 1402
Size: 4952
AlwNI CAG_nnn'CTG
Cuts at: 441 1374 1477 2257 3824 441
Size: 933 103 780 1567 1569
Fragments arranged by size:
1569 1567 933 780 103
ApaI G_GGCC'C
Cuts at: 245 245
Size: 4952
ApaLI G'TGCA_C
Cuts at: 3722 3722
Size: 4952
ApoI r'AATT_y
Cuts at: 1956 4305 4561 1956
Size: 2349 256 2347
Fragments arranged by size:
2349 2347 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4952
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4952
AvaI C'yCGr_G
Cuts at: 173 291 324 385 1972 173
Size: 118 33 61 1587 3153
Fragments arranged by size:
3153 1587 118 61 33
BamHI G'GATC_C
Cuts at: 1789 1977 1789
Size: 188 4764
BanI G'GyrC_C
Cuts at: 123 142 255 514 966 1044 1225 1968 2382
Size: 19 113 259 452 78 181 743 414
Cuts at: 2382 2630 2665 123
Size: 248 35 2410
Fragments arranged by size:
2410 743 452 414 259 248 181 113
78 35 19
BanII G_rGCy'C
Cuts at: 209 233 245 844 1600 1966 2996 209
Size: 24 12 599 756 366 1030 2165
Fragments arranged by size:
2165 1030 756 599 366 24 12
BbeI G_GCGC'C
Cuts at: 259 970 2386 2634 259
Size: 711 1416 248 2577
Fragments arranged by size:
2577 1416 711 248
BciVI GTATCCnnnnn_n'
Cuts at: 640 842 2850 3611 4952 640
Size: 202 2008 761 1341 640
Fragments arranged by size:
2008 1341 761 640 202
BclI T'GATC_A
Cuts at: 2472 2472
Size: 4952
BglI GCCn_nnn'nGGC
Cuts at: 264 375 997 264
Size: 111 622 4219
Fragments arranged by size:
4219 622 111
BglII A'GATC_T
Cuts at: 2467 4504 2467
Size: 2037 2915
BlpI GC'TnA_GC
Cuts at: 232 2064 232
Size: 1832 3120
Bme1580I G_kGCm'C
Cuts at: 128 245 424 448 517 1228 1646 1729 1836
Size: 117 179 24 69 711 418 83 107
Cuts at: 1836 2577 2670 3726 128
Size: 741 93 1056 1354
Fragments arranged by size:
1354 1056 741 711 418 179 117 107
93 83 69 24
BmgBI CAC'GTC
Cuts at: 690 690
Size: 4952
BmrI ACTGGGnnnn_n'
Cuts at: 2164 2245 2580 4724 2164
Size: 81 335 2144 2392
Fragments arranged by size:
2392 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3319 3319
Size: 4952
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 151 193 1437 1837 151
Size: 42 1244 400 3266
Fragments arranged by size:
3266 1244 400 42
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2128 3007 3499 3797 2128
Size: 879 492 298 3283
Fragments arranged by size:
3283 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 910 1084 4656 910
Size: 174 3572 1206
Fragments arranged by size:
3572 1206 174
BsaAI yAC'GTr
Cuts at: 1097 2305 1097
Size: 1208 3744
BsaBI GATnn'nnATC
Cuts at: 6 2466 4503 6
Size: 2460 2037 455
Fragments arranged by size:
2460 2037 455
BsaHI Gr'CG_yC
Cuts at: 256 451 967 1514 2383 2631 3333 4829 256
Size: 195 516 547 869 248 702 1496 379
Fragments arranged by size:
1496 869 702 547 516 379 248 195
BsaWI w'CCGG_w
Cuts at: 834 2141 2662 3340 3614 3761 4172 834
Size: 1307 521 678 274 147 411 1614
Fragments arranged by size:
1614 1307 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1081 1111 1081
Size: 30 4922
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 173 370 409 790 1274 1711 1772 173
Size: 197 39 381 484 437 61 3353
Fragments arranged by size:
3353 484 437 381 197 61 39
BseYI C'CCAG_C
Cuts at: 226 510 616 720 1147 1407 1804 2377 3135
Size: 284 106 104 427 260 397 573 758
Cuts at: 3135 3712 4208 226
Size: 577 496 970
Fragments arranged by size:
970 758 577 573 496 427 397 284
260 106 104
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 442 442
Size: 4952
BsiEI CG_ry'CG
Cuts at: 104 379 606 2494 2540 3748 104
Size: 275 227 1888 46 1208 1308
Fragments arranged by size:
1888 1308 1208 275 227 46
BsiHKAI G_wGCw'C
Cuts at: 209 844 1966 2744 2934 3726 209
Size: 635 1122 778 190 792 1435
Fragments arranged by size:
1435 1122 792 778 635 190
BsmI GAATG_Cn'
Cuts at: 1827 1827
Size: 4952
BsmBI CGTCTCn'nnnn_
Cuts at: 275 496 684 876 1659 275
Size: 221 188 192 783 3568
Fragments arranged by size:
3568 783 221 192 188
Bsp1286I G_dGCh'C
Cuts at: 128 209 233 245 424 448 517 844 1228
Size: 81 24 12 179 24 69 327 384
Cuts at: 1228 1600 1646 1729 1836 1966 2577 2670 2744
Size: 372 46 83 107 130 611 93 74
Cuts at: 2744 2934 2996 3726 128
Size: 190 62 730 1354
Fragments arranged by size:
1354 730 611 384 372 327 190 179
130 107 93 83 81 74 69 62
46 24 24 12
BspEI T'CCGG_A
Cuts at: 834 4172 834
Size: 3338 1614
BspHI T'CATG_A
Cuts at: 1175 4128 1175
Size: 2953 1999
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2002 2216 2518 2899 2002
Size: 214 302 381 4055
Fragments arranged by size:
4055 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1050 3244 3298 4782 4947 50
Size: 1000 2194 54 1484 165 55
Fragments arranged by size:
2194 1484 1000 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1628 2864 1628
Size: 1236 3716
BsrFI r'CCGG_y
Cuts at: 252 611 669 1701 2950 3131 3340 252
Size: 359 58 1032 1249 181 209 1864
Fragments arranged by size:
1864 1249 1032 359 209 181 58
BssHII G'CGCG_C
Cuts at: 272 272
Size: 4952
BssSI C'ACGA_G
Cuts at: 1126 1693 3223 3581 1126
Size: 567 1530 358 2497
Fragments arranged by size:
2497 1530 567 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1831 2329 1831
Size: 498 4454
BstBI TT'CG_AA
Cuts at: 3313 3313
Size: 4952
BstEII G'GTnAC_C
Cuts at: 1533 1533
Size: 4952
BstXI CCAn_nnnn'nTGG
Cuts at: 1065 1293 1065
Size: 228 4724
BstYI r'GATC_y
Cuts at: 1 1370 1789 1977 2444 2467 2802 3048 4060
Size: 1369 419 188 467 23 335 246 1012
Cuts at: 4060 4146 4158 4504 1
Size: 86 12 346 449
Fragments arranged by size:
1369 1012 467 449 419 346 335 246
188 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 583 583
Size: 4952
BtgI C'CryG_G
Cuts at: 107 364 734 107
Size: 257 370 4325
Fragments arranged by size:
4325 370 257
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 179 251 846 1596 2902 3083 3246 179
Size: 72 595 750 1306 181 163 1885
Fragments arranged by size:
1885 1306 750 595 181 163 72
BtsI GCAGTG_nn'
Cuts at: 1535 2195 1535
Size: 660 4292
ClaI AT'CG_AT
Cuts at: 1187 1187
Size: 4952
DraI TTT'AAA
Cuts at: 1951 1951
Size: 4952
DrdI GACnn_nn'nnGTC
Cuts at: 1759 2658 3516 1759
Size: 899 858 3195
Fragments arranged by size:
3195 899 858
EaeI y'GGCC_r
Cuts at: 186 376 1067 1196 1266 1295 2537 2711 3102
Size: 190 691 129 70 29 1242 174 391
Cuts at: 3102 3129 4474 186
Size: 27 1345 664
Fragments arranged by size:
1345 1242 691 664 391 190 174 129
70 29 27
EagI C'GGCC_G
Cuts at: 376 2537 376
Size: 2161 2791
EarI CTCTTCn'nnn_
Cuts at: 767 1435 1453 1712 2975 3185 767
Size: 668 18 259 1263 210 2534
Fragments arranged by size:
2534 1263 668 259 210 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 943 1271 1303 3160 3470 3616 4762 943
Size: 328 32 1857 310 146 1146 1133
Fragments arranged by size:
1857 1146 1133 328 310 146 32
EcoICRI GAG'CTC
Cuts at: 207 842 1964 207
Size: 635 1122 3195
Fragments arranged by size:
3195 1122 635
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 151 193 1437 1837 2777 3209 3956 4224 151
Size: 42 1244 400 940 432 747 268 879
Fragments arranged by size:
1244 940 879 747 432 400 268 42
EcoNI CCTnn'n_nnAGG
Cuts at: 1398 1398
Size: 4952
EcoO109I rG'GnC_Cy
Cuts at: 549 1061 1620 1811 1901 2091 549
Size: 512 559 191 90 190 3410
Fragments arranged by size:
3410 559 512 191 190 90
EcoRI G'AATT_C
Cuts at: 1956 1956
Size: 4952
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2971 2971
Size: 4952
FspI TGC'GCA
Cuts at: 2733 2733
Size: 4952
HaeII r_GCGC'y
Cuts at: 259 564 744 810 970 2386 2634 3656 4635
Size: 305 180 66 160 1416 248 1022 979
Cuts at: 4635 259
Size: 576
Fragments arranged by size:
1416 1022 979 576 305 248 180 160
66
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 274 306 2794 2824 2826 2856 274
Size: 32 2488 30 2 30 2370
Fragments arranged by size:
2488 2370 32 30 30 2
HincII GTy'rAC
Cuts at: 675 1991 675
Size: 1316 3636
KasI G'GCGC_C
Cuts at: 255 966 2382 2630 255
Size: 711 1416 248 2577
Fragments arranged by size:
2577 1416 711 248
KpnI G_GTAC'C
Cuts at: 1972 1972
Size: 4952
MluI A'CGCG_T
Cuts at: 4385 4385
Size: 4952
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1682 3623 3807 1682
Size: 1941 184 2827
Fragments arranged by size:
2827 1941 184
MscI TGG'CCA
Cuts at: 1198 1297 2713 1198
Size: 99 1416 3437
Fragments arranged by size:
3437 1416 99
MslI CAynn'nnrTG
Cuts at: 1189 2941 3068 1189
Size: 1752 127 3073
Fragments arranged by size:
3073 1752 127
MspA1I CmG'CkG
Cuts at: 315 348 510 720 1010 1767 1778 1847 2063
Size: 33 162 210 290 757 11 69 216
Cuts at: 2063 2377 2737 3750 3995 315
Size: 314 360 1013 245 1272
Fragments arranged by size:
1272 1013 757 360 314 290 245 216
210 162 69 33 11
NaeI GCC'GGC
Cuts at: 254 1703 3133 254
Size: 1449 1430 2073
Fragments arranged by size:
2073 1449 1430
NarI GG'CG_CC
Cuts at: 256 967 2383 2631 256
Size: 711 1416 248 2577
Fragments arranged by size:
2577 1416 711 248
NcoI C'CATG_G
Cuts at: 107 734 107
Size: 627 4325
NgoMIV G'CCGG_C
Cuts at: 252 1701 3131 252
Size: 1449 1430 2073
Fragments arranged by size:
2073 1449 1430
NspI r_CATG'y
Cuts at: 2005 2505 3412 2005
Size: 500 907 3545
Fragments arranged by size:
3545 907 500
PasI CC'CwG_GG
Cuts at: 994 1221 994
Size: 227 4725
PciI A'CATG_T
Cuts at: 3408 3408
Size: 4952
PflMI CCAn_nnn'nTGG
Cuts at: 441 441
Size: 4952
PfoI T'CCnGG_A
Cuts at: 1165 1165
Size: 4952
PmeI GTTT'AAAC
Cuts at: 1951 1951
Size: 4952
PmlI CAC'GTG
Cuts at: 1097 1097
Size: 4952
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4091 4123 4091
Size: 32 4920
PpuMI rG'GwC_Cy
Cuts at: 549 1061 1620 1811 1901 549
Size: 512 559 191 90 3600
Fragments arranged by size:
3600 559 512 191 90
PspOMI G'GGCC_C
Cuts at: 241 241
Size: 4952
PstI C_TGCA'G
Cuts at: 1370 1999 2684 1370
Size: 629 685 3638
Fragments arranged by size:
3638 685 629
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4952
PvuII CAG'CTG
Cuts at: 510 720 1847 2377 2737 510
Size: 210 1127 530 360 2725
Fragments arranged by size:
2725 1127 530 360 210
RsrII CG'GwC_CG
Cuts at: 3147 3147
Size: 4952
SacI G_AGCT'C
Cuts at: 209 844 1966 209
Size: 635 1122 3195
Fragments arranged by size:
3195 1122 635
SalI G'TCGA_C
Cuts at: 1989 1989
Size: 4952
SanDI GG'GwC_CC
Cuts at: 549 1811 549
Size: 1262 3690
SapI GCTCTTCn'nnn_
Cuts at: 1712 2975 3185 1712
Size: 1263 210 3479
Fragments arranged by size:
3479 1263 210
SbfI CC_TGCA'GG
Cuts at: 1999 1999
Size: 4952
ScaI AGT'ACT
Cuts at: 595 4469 595
Size: 3874 1078
SfcI C'TryA_G
Cuts at: 33 1366 1995 2680 3673 3864 33
Size: 1333 629 685 993 191 1121
Fragments arranged by size:
1333 1121 993 685 629 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 375 375
Size: 4952
SfoI GGC'GCC
Cuts at: 257 968 2384 2632 257
Size: 711 1416 248 2577
Fragments arranged by size:
2577 1416 711 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4952
SmaI CCC'GGG
Cuts at: 175 326 1974 175
Size: 151 1648 3153
Fragments arranged by size:
3153 1648 151
SmlI C'TyrA_G
Cuts at: 1711 2107 3022 3514 3776 1711
Size: 396 915 492 262 2887
Fragments arranged by size:
2887 915 492 396 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4952
SphI G_CATG'C
Cuts at: 2005 2505 2005
Size: 500 4452
SrfI GCCC'GGGC
Cuts at: 175 175
Size: 4952
StuI AGG'CCT
Cuts at: 1393 1393
Size: 4952
StyI C'CwwG_G
Cuts at: 107 245 734 1358 2086 2440 107
Size: 138 489 624 728 354 2619
Fragments arranged by size:
2619 728 624 489 354 138
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 864 2535 2668 3127 3336 864
Size: 1671 133 459 209 2480
Fragments arranged by size:
2480 1671 459 209 133
TatI w'GTAC_w
Cuts at: 593 4467 593
Size: 3874 1078
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1081 1113 4469 4501 1081
Size: 32 3356 32 1532
Fragments arranged by size:
3356 1532 32 32
Tth111I GACn'n_nGTC
Cuts at: 2749 2749
Size: 4952
XbaI T'CTAG_A
Cuts at: 1983 1983
Size: 4952
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 540 1404 1545 1884 2447 540
Size: 864 141 339 563 3045
Fragments arranged by size:
3045 864 563 339 141
XmaI C'CCGG_G
Cuts at: 173 324 1972 173
Size: 151 1648 3153
Fragments arranged by size:
3153 1648 151
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SmaI SmlI SpeI SphI SrfI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AhdI AleI AloI AscI AvrII BaeI
BbsI BbvCI BcgI BfrBI BmtI Bpu10I BsiWI BsrGI
BstZ17I CspCI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PshAI
PsiI PspXI PsrI SacII SexAI SgrAI SnaBI SspI
SwaI XhoI XmnI ZraI