Restriction map for pF1KB9882
 (Linear) (Six-base) MAPSORT of: KIBB9882.seq  Check: 7248  from: 1  to: 1839



KIBB9882.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:12  ..

AccI GT'mk_AC

Cuts at:      0    1355    1839 
   Size:       1355     484
 
AfeI AGC'GCT

Cuts at:      0     634    1839 
   Size:        634    1205
 
AflII C'TTAA_G

Cuts at:      0    1603    1839 
   Size:       1603     236
 
AflIII A'CryG_T

Cuts at:      0     581    1839 
   Size:        581    1258
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1294    1839 
   Size:       1294     545
 
AlwNI CAG_nnn'CTG

Cuts at:      0     333    1266    1369    1839 
   Size:        333     933     103     470

  Fragments arranged by size: 

                 933     470     333     103
 
ApaI G_GGCC'C

Cuts at:      0     137    1839 
   Size:        137    1702
 
AvaI C'yCGr_G

Cuts at:      0      65     183     216     277    1839 
   Size:         65     118      33      61    1562

  Fragments arranged by size: 

                1562     118      65      61      33
 
BamHI G'GATC_C

Cuts at:      0    1681    1839 
   Size:       1681     158
 
BanI G'GyrC_C

Cuts at:      0      15      34     147     406     858     936    1117    1839 
   Size:         15      19     113     259     452      78     181     722

  Fragments arranged by size: 

                 722     452     259     181     113      78      19      15
            
 
BanII G_rGCy'C

Cuts at:      0     101     125     137     736    1492    1839 
   Size:        101      24      12     599     756     347

  Fragments arranged by size: 

                 756     599     347     101      24      12
 
BbeI G_GCGC'C

Cuts at:      0     151     862    1839 
   Size:        151     711     977

  Fragments arranged by size: 

                 977     711     151
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     532     734    1839 
   Size:        532     202    1105

  Fragments arranged by size: 

                1105     532     202
 
BglI GCCn_nnn'nGGC

Cuts at:      0     156     267     889    1839 
   Size:        156     111     622     950

  Fragments arranged by size: 

                 950     622     156     111
 
BlpI GC'TnA_GC

Cuts at:      0     124    1839 
   Size:        124    1715
 
Bme1580I G_kGCm'C

Cuts at:      0      20     137     316     340     409    1120    1538    1621 
   Size:         20     117     179      24      69     711     418      83

Cuts at:   1621    1728    1839 
   Size:        107     111

  Fragments arranged by size: 

                 711     418     179     117     111     107      83      69
                  24      20
 
BmgBI CAC'GTC

Cuts at:      0     582    1839 
   Size:        582    1257
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      43      85    1329    1729    1839 
   Size:         43      42    1244     400     110

  Fragments arranged by size: 

                1244     400     110      43      42
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     802     976    1839 
   Size:        802     174     863

  Fragments arranged by size: 

                 863     802     174
 
BsaAI yAC'GTr

Cuts at:      0     989    1839 
   Size:        989     850
 
BsaHI Gr'CG_yC

Cuts at:      0     148     343     859    1406    1839 
   Size:        148     195     516     547     433

  Fragments arranged by size: 

                 547     516     433     195     148
 
BsaWI w'CCGG_w

Cuts at:      0     726    1839 
   Size:        726    1113
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     973    1003    1839 
   Size:        973      30     836

  Fragments arranged by size: 

                 973     836      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      65     262     301     682    1166    1603    1664    1839 
   Size:         65     197      39     381     484     437      61     175

  Fragments arranged by size: 

                 484     437     381     197     175      65      61      39
            
 
BseYI C'CCAG_C

Cuts at:      0     118     402     508     612    1039    1299    1696    1839 
   Size:        118     284     106     104     427     260     397     143

  Fragments arranged by size: 

                 427     397     284     260     143     118     106     104
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     334    1839 
   Size:        334    1505
 
BsiEI CG_ry'CG

Cuts at:      0     271     498    1839 
   Size:        271     227    1341

  Fragments arranged by size: 

                1341     271     227
 
BsiHKAI G_wGCw'C

Cuts at:      0     101     736    1839 
   Size:        101     635    1103

  Fragments arranged by size: 

                1103     635     101
 
BsmI GAATG_Cn'

Cuts at:      0    1719    1839 
   Size:       1719     120
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     167     388     576     768    1551    1839 
   Size:        167     221     188     192     783     288

  Fragments arranged by size: 

                 783     288     221     192     188     167
 
Bsp1286I G_dGCh'C

Cuts at:      0      20     101     125     137     316     340     409     736 
   Size:         20      81      24      12     179      24      69     327

Cuts at:    736    1120    1492    1538    1621    1728    1839 
   Size:        384     372      46      83     107     111

  Fragments arranged by size: 

                 384     372     327     179     111     107      83      81
                  69      46      24      24      20      12
 
BspEI T'CCGG_A

Cuts at:      0     726    1839 
   Size:        726    1113
 
BspHI T'CATG_A

Cuts at:      0    1067    1839 
   Size:       1067     772
 
BsrBI CCG'CTC

Cuts at:      0     942    1839 
   Size:        942     897
 
BsrDI GCAATG_nn'

Cuts at:      0    1520    1839 
   Size:       1520     319
 
BsrFI r'CCGG_y

Cuts at:      0     144     503     561    1593    1839 
   Size:        144     359      58    1032     246

  Fragments arranged by size: 

                1032     359     246     144      58
 
BssHII G'CGCG_C

Cuts at:      0     164    1839 
   Size:        164    1675
 
BssSI C'ACGA_G

Cuts at:      0    1018    1585    1839 
   Size:       1018     567     254

  Fragments arranged by size: 

                1018     567     254
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1723    1839 
   Size:       1723     116
 
BstEII G'GTnAC_C

Cuts at:      0    1425    1839 
   Size:       1425     414
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     957    1185    1839 
   Size:        957     228     654

  Fragments arranged by size: 

                 957     654     228
 
BstYI r'GATC_y

Cuts at:      0    1262    1681    1839 
   Size:       1262     419     158

  Fragments arranged by size: 

                1262     419     158
 
Bsu36I CC'TnA_GG

Cuts at:      0     475    1839 
   Size:        475    1364
 
BtgI C'CryG_G

Cuts at:      0     256     626    1839 
   Size:        256     370    1213

  Fragments arranged by size: 

                1213     370     256
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      71     143     738    1488    1839 
   Size:         71      72     595     750     351

  Fragments arranged by size: 

                 750     595     351      72      71
 
BtsI GCAGTG_nn'

Cuts at:      0    1427    1839 
   Size:       1427     412
 
ClaI AT'CG_AT

Cuts at:      0    1079    1839 
   Size:       1079     760
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1651    1839 
   Size:       1651     188
 
EaeI y'GGCC_r

Cuts at:      0      78     268     959    1088    1158    1187    1839 
   Size:         78     190     691     129      70      29     652

  Fragments arranged by size: 

                 691     652     190     129      78      70      29
 
EagI C'GGCC_G

Cuts at:      0     268    1839 
   Size:        268    1571
 
EarI CTCTTCn'nnn_

Cuts at:      0     659    1327    1345    1604    1839 
   Size:        659     668      18     259     235

  Fragments arranged by size: 

                 668     659     259     235      18
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     835    1163    1195    1839 
   Size:        835     328      32     644

  Fragments arranged by size: 

                 835     644     328      32
 
EcoICRI GAG'CTC

Cuts at:      0      99     734    1839 
   Size:         99     635    1105

  Fragments arranged by size: 

                1105     635      99
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      43      85    1329    1729    1839 
   Size:         43      42    1244     400     110

  Fragments arranged by size: 

                1244     400     110      43      42
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1290    1839 
   Size:       1290     549
 
EcoO109I rG'GnC_Cy

Cuts at:      0     441     953    1512    1703    1793    1839 
   Size:        441     512     559     191      90      46

  Fragments arranged by size: 

                 559     512     441     191      90      46
 
HaeII r_GCGC'y

Cuts at:      0     151     456     636     702     862    1839 
   Size:        151     305     180      66     160     977

  Fragments arranged by size: 

                 977     305     180     160     151      66
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     166     198    1839 
   Size:        166      32    1641

  Fragments arranged by size: 

                1641     166      32
 
HincII GTy'rAC

Cuts at:      0     567    1839 
   Size:        567    1272
 
KasI G'GCGC_C

Cuts at:      0     147     858    1839 
   Size:        147     711     981

  Fragments arranged by size: 

                 981     711     147
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1574    1839 
   Size:       1574     265
 
MscI TGG'CCA

Cuts at:      0    1090    1189    1839 
   Size:       1090      99     650

  Fragments arranged by size: 

                1090     650      99
 
MslI CAynn'nnrTG

Cuts at:      0    1081    1839 
   Size:       1081     758
 
MspA1I CmG'CkG

Cuts at:      0     207     240     402     612     902    1659    1670    1739 
   Size:        207      33     162     210     290     757      11      69

Cuts at:   1739    1839 
   Size:        100

  Fragments arranged by size: 

                 757     290     210     207     162     100      69      33
                  11
 
NaeI GCC'GGC

Cuts at:      0     146    1595    1839 
   Size:        146    1449     244

  Fragments arranged by size: 

                1449     244     146
 
NarI GG'CG_CC

Cuts at:      0     148     859    1839 
   Size:        148     711     980

  Fragments arranged by size: 

                 980     711     148
 
NcoI C'CATG_G

Cuts at:      0     626    1839 
   Size:        626    1213
 
NgoMIV G'CCGG_C

Cuts at:      0     144    1593    1839 
   Size:        144    1449     246

  Fragments arranged by size: 

                1449     246     144
 
PasI CC'CwG_GG

Cuts at:      0     886    1113    1839 
   Size:        886     227     726

  Fragments arranged by size: 

                 886     726     227
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     333    1839 
   Size:        333    1506
 
PfoI T'CCnGG_A

Cuts at:      0    1057    1839 
   Size:       1057     782
 
PmlI CAC'GTG

Cuts at:      0     989    1839 
   Size:        989     850
 
PpuMI rG'GwC_Cy

Cuts at:      0     441     953    1512    1703    1793    1839 
   Size:        441     512     559     191      90      46

  Fragments arranged by size: 

                 559     512     441     191      90      46
 
PspOMI G'GGCC_C

Cuts at:      0     133    1839 
   Size:        133    1706
 
PstI C_TGCA'G

Cuts at:      0    1262    1839 
   Size:       1262     577
 
PvuII CAG'CTG

Cuts at:      0     402     612    1739    1839 
   Size:        402     210    1127     100

  Fragments arranged by size: 

                1127     402     210     100
 
SacI G_AGCT'C

Cuts at:      0     101     736    1839 
   Size:        101     635    1103

  Fragments arranged by size: 

                1103     635     101
 
SanDI GG'GwC_CC

Cuts at:      0     441    1703    1839 
   Size:        441    1262     136

  Fragments arranged by size: 

                1262     441     136
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1604    1839 
   Size:       1604     235
 
ScaI AGT'ACT

Cuts at:      0     487    1839 
   Size:        487    1352
 
SfcI C'TryA_G

Cuts at:      0    1258    1839 
   Size:       1258     581
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     267    1839 
   Size:        267    1572
 
SfoI GGC'GCC

Cuts at:      0     149     860    1839 
   Size:        149     711     979

  Fragments arranged by size: 

                 979     711     149
 
SmaI CCC'GGG

Cuts at:      0      67     218    1839 
   Size:         67     151    1621

  Fragments arranged by size: 

                1621     151      67
 
SmlI C'TyrA_G

Cuts at:      0    1603    1839 
   Size:       1603     236
 
SrfI GCCC'GGGC

Cuts at:      0      67    1839 
   Size:         67    1772
 
StuI AGG'CCT

Cuts at:      0    1285    1839 
   Size:       1285     554
 
StyI C'CwwG_G

Cuts at:      0     137     626    1250    1839 
   Size:        137     489     624     589

  Fragments arranged by size: 

                 624     589     489     137
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     756    1839 
   Size:        756    1083
 
TatI w'GTAC_w

Cuts at:      0     485    1839 
   Size:        485    1354
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     973    1005    1839 
   Size:        973      32     834

  Fragments arranged by size: 

                 973     834      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     432    1296    1437    1776    1839 
   Size:        432     864     141     339      63

  Fragments arranged by size: 

                 864     432     339     141      63
 
XmaI C'CCGG_G

Cuts at:      0      65     216    1839 
   Size:         65     151    1623

  Fragments arranged by size: 

                1623     151      65
 

 Enzymes that do cut:

     AccI     AfeI    AflII   AflIII     AlfI    AlwNI     ApaI     AvaI
    BamHI     BanI    BanII     BbeI    BciVI     BglI     BlpI Bme1580I
    BmgBI     BpmI     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI
    BspHI    BsrBI    BsrDI    BsrFI   BssHII    BssSI   BstAPI   BstEII
    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI     ClaI     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    HaeII    Hin4I   HincII     KasI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI   NgoMIV     PasI    PflMI     PfoI     PmlI
    PpuMI   PspOMI     PstI    PvuII     SacI    SanDI     SapI     ScaI
     SfcI     SfiI     SfoI     SmaI     SmlI     SrfI     StuI     StyI
    TaqII     TatI     TstI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AgeI     AhdI     AleI
     AloI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI
     BbsI    BbvCI     BcgI     BclI    BfrBI    BglII     BmrI     BmtI
     BplI   Bpu10I    BpuEI    BsaBI    BsiWI    BspMI    BsrGI    BstBI
  BstZ17I    CspCI     DraI   DraIII    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI  HindIII     HpaI     KpnI     MfeI     MluI     NdeI
     NheI     NotI     NruI     NsiI     NspI     PacI     PciI     PmeI
     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII    SacII
     SalI     SbfI    SexAI     SgfI    SgrAI    SnaBI     SpeI     SphI
     SspI     SwaI  Tth111I     XbaI     XhoI     XmnI     ZraI
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