Restriction map for pF1KB9874
 (Linear) (Six-base) MAPSORT of: KIBB9874.seq  Check: 4571  from: 1  to: 1545



KIBB9874.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:12  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     231    1545 
   Size:        231    1314
 
AccI GT'mk_AC

Cuts at:      0     272    1545 
   Size:        272    1273
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     143     844    1139    1522    1545 
   Size:        143     701     295     383      23

  Fragments arranged by size: 

                 701     383     295     143      23
 
AleI CACnn'nnGTG

Cuts at:      0     469     883    1545 
   Size:        469     414     662

  Fragments arranged by size: 

                 662     469     414
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1350    1382    1545 
   Size:       1350      32     163

  Fragments arranged by size: 

                1350     163      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1457    1545 
   Size:       1457      88
 
ApoI r'AATT_y

Cuts at:      0     686     760     816     900    1545 
   Size:        686      74      56      84     645

  Fragments arranged by size: 

                 686     645      84      74      56
 
AvrII C'CTAG_G

Cuts at:      0     376     696    1545 
   Size:        376     320     849

  Fragments arranged by size: 

                 849     376     320
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     248    1545 
   Size:        248    1297
 
BfrBI ATG'CAT

Cuts at:      0     286     959    1545 
   Size:        286     673     586

  Fragments arranged by size: 

                 673     586     286
 
BglII A'GATC_T

Cuts at:      0      68    1533    1545 
   Size:         68    1465      12

  Fragments arranged by size: 

                1465      68      12
 
Bme1580I G_kGCm'C

Cuts at:      0    1351    1545 
   Size:       1351     194
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     240    1545 
   Size:        240    1305
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      52     373     436    1545 
   Size:         52     321      63    1109

  Fragments arranged by size: 

                1109     321      63      52
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     328     591    1312    1545 
   Size:        328     263     721     233

  Fragments arranged by size: 

                 721     328     263     233
 
BsaAI yAC'GTr

Cuts at:      0    1488    1545 
   Size:       1488      57
 
BsaBI GATnn'nnATC

Cuts at:      0     851    1545 
   Size:        851     694
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      34      64     349     379    1350    1380    1545 
   Size:         34      30     285      30     971      30     165

  Fragments arranged by size: 

                 971     285     165      34      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     370     402    1545 
   Size:        370      32    1143

  Fragments arranged by size: 

                1143     370      32
 
BsiHKAI G_wGCw'C

Cuts at:      0     808    1545 
   Size:        808     737
 
BsmI GAATG_Cn'

Cuts at:      0     104    1545 
   Size:        104    1441
 
Bsp1286I G_dGCh'C

Cuts at:      0     808    1351    1545 
   Size:        808     543     194

  Fragments arranged by size: 

                 808     543     194
 
BspHI T'CATG_A

Cuts at:      0     512    1545 
   Size:        512    1033
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     231    1545 
   Size:        231    1314
 
BsrBI CCG'CTC

Cuts at:      0    1163    1545 
   Size:       1163     382
 
BstBI TT'CG_AA

Cuts at:      0      96    1545 
   Size:         96    1449
 
BstEII G'GTnAC_C

Cuts at:      0    1090    1156    1307    1321    1545 
   Size:       1090      66     151      14     224

  Fragments arranged by size: 

                1090     224     151      66      14
 
BstYI r'GATC_y

Cuts at:      0      68    1533    1545 
   Size:         68    1465      12

  Fragments arranged by size: 

                1465      68      12
 
BtgI C'CryG_G

Cuts at:      0     567    1255    1545 
   Size:        567     688     290

  Fragments arranged by size: 

                 688     567     290
 
BtsI GCAGTG_nn'

Cuts at:      0     878     890    1406    1545 
   Size:        878      12     516     139

  Fragments arranged by size: 

                 878     516     139      12
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0      42      77    1545 
   Size:         42      35    1468

  Fragments arranged by size: 

                1468      42      35
 
DraI TTT'AAA

Cuts at:      0     705     899    1545 
   Size:        705     194     646

  Fragments arranged by size: 

                 705     646     194
 
DraIII CAC_nnn'GTG

Cuts at:      0    1491    1545 
   Size:       1491      54
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     601    1545 
   Size:        601     944
 
EaeI y'GGCC_r

Cuts at:      0     570    1545 
   Size:        570     975
 
EarI CTCTTCn'nnn_

Cuts at:      0     308    1545 
   Size:        308    1237
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      52     143     373     436     844    1139    1522    1545 
   Size:         52      91     230      63     408     295     383      23

  Fragments arranged by size: 

                 408     383     295     230      91      63      52      23
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1316    1373    1545 
   Size:       1316      57     172

  Fragments arranged by size: 

                1316     172      57
 
EcoRI G'AATT_C

Cuts at:      0     760    1545 
   Size:        760     785
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     130     162     318     350    1372    1404    1545 
   Size:        130      32     156      32    1022      32     141

  Fragments arranged by size: 

                1022     156     141     130      32      32      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     486    1545 
   Size:        486    1059
 
MscI TGG'CCA

Cuts at:      0     572    1545 
   Size:        572     973
 
MslI CAynn'nnrTG

Cuts at:      0     469     883    1545 
   Size:        469     414     662

  Fragments arranged by size: 

                 662     469     414
 
MspA1I CmG'CkG

Cuts at:      0     121     414    1257    1545 
   Size:        121     293     843     288

  Fragments arranged by size: 

                 843     293     288     121
 
NcoI C'CATG_G

Cuts at:      0     567    1545 
   Size:        567     978
 
NsiI A_TGCA'T

Cuts at:      0     288     961    1545 
   Size:        288     673     584

  Fragments arranged by size: 

                 673     584     288
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1101    1545 
   Size:       1101     444
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1132    1164    1350    1382    1545 
   Size:       1132      32     186      32     163

  Fragments arranged by size: 

                1132     186     163      32      32
 
PsiI TTA'TAA

Cuts at:      0     851    1545 
   Size:        851     694
 
PstI C_TGCA'G

Cuts at:      0     184     242     885    1545 
   Size:        184      58     643     660

  Fragments arranged by size: 

                 660     643     184      58
 
PvuII CAG'CTG

Cuts at:      0     121     414    1545 
   Size:        121     293    1131

  Fragments arranged by size: 

                1131     293     121
 
SacII CC_GC'GG

Cuts at:      0    1258    1545 
   Size:       1258     287
 
ScaI AGT'ACT

Cuts at:      0    1011    1545 
   Size:       1011     534
 
SfcI C'TryA_G

Cuts at:      0     180     238     650     881    1181    1286    1545 
   Size:        180      58     412     231     300     105     259

  Fragments arranged by size: 

                 412     300     259     231     180     105      58
 
SmlI C'TyrA_G

Cuts at:      0     307     606    1327    1545 
   Size:        307     299     721     218

  Fragments arranged by size: 

                 721     307     299     218
 
SpeI A'CTAG_T

Cuts at:      0    1012    1545 
   Size:       1012     533
 
SspI AAT'ATT

Cuts at:      0     629    1070    1545 
   Size:        629     441     475

  Fragments arranged by size: 

                 629     475     441
 
StuI AGG'CCT

Cuts at:      0     205    1545 
   Size:        205    1340
 
StyI C'CwwG_G

Cuts at:      0      18     376     567     696    1545 
   Size:         18     358     191     129     849

  Fragments arranged by size: 

                 849     358     191     129      18
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     100    1545 
   Size:        100    1445
 
TatI w'GTAC_w

Cuts at:      0     981    1009    1545 
   Size:        981      28     536

  Fragments arranged by size: 

                 981     536      28
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      34      66    1477    1509    1545 
   Size:         34      32    1411      32      36

  Fragments arranged by size: 

                1411      36      34      32      32
 
XbaI T'CTAG_A

Cuts at:      0    1150    1545 
   Size:       1150     395
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AleI     AloI    AlwNI     ApoI    AvrII
     BbsI    BfrBI    BglII Bme1580I     BmrI     BpmI    BpuEI    BsaAI
    BsaBI    BsaXI    BseRI  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI
    BsrBI    BstBI   BstEII    BstYI     BtgI     BtsI    CspCI     DraI
   DraIII     DrdI     EaeI     EarI  Eco57MI    EcoNI    EcoRI    Hin4I
     MmeI     MscI     MslI   MspA1I     NcoI     NsiI    PflMI     PpiI
     PsiI     PstI    PvuII    SacII     ScaI     SfcI     SmlI     SpeI
     SspI     StuI     StyI    TaqII     TatI     TstI     XbaI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AlfI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI     BaeI
    BamHI     BanI    BanII     BbeI    BbvCI     BcgI    BciVI     BclI
     BglI     BlpI    BmgBI     BmtI     BplI   Bpu10I     BsaI    BsaHI
    BsaWI    BseYI     BsgI    BsiEI    BsiWI    BsmBI    BspEI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstXI  BstZ17I   Bsu36I
    BtgZI     ClaI     EagI     EciI  EcoICRI EcoO109I    EcoRV     FalI
     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     NaeI     NarI     NdeI   NgoMIV     NheI
     NotI     NruI     NspI     PacI     PasI     PciI     PfoI     PmeI
     PmlI    PpuMI    PshAI   PspOMI    PspXI     PsrI     PvuI    RsrII
     SacI     SalI    SanDI     SapI     SbfI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SphI     SrfI     SwaI  Tth111I
     XcmI     XhoI     XmaI     XmnI     ZraI
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