Restriction map for pF1KB9864
##################################
# NO CUT ENZYMES (  129 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AgeI        AhdI        AocI        
ApaI        ApoI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AviII       AvrII       
BamHI       BanII       BbrPI       BbvCI       BcgI        
BciVI       BfaI        BfrI        BglI        BglII       
BlnI        BsaBI       BseAI       BseRI       BsiEI       
BsiWI       BsmBI       BsmFI       BsmI        Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BsrGI       
BssSI       Bst1107I    BstBI       BstYI       BstZ17I     
Bsu36I      ClaI        CpoI        DraI        DraII       
EagI        Eam1105I    EclXI       Eco52I      Eco81I      
EcoNI       EcoO109I    EcoRI       EcoRV       EcoT22I     
FseI        FspI        HincII      HindII      HindIII     
HpaI        KpnI        KspI        MaeI        MamI        
MflI        MroI        NaeI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfiI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        Sse8387I    
SspBI       SspI        StuI        SwaI        TfiI        
Tth111I     Van91I      XbaI        XcmI        XhoI        
XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AfaI          402  GTAC                 GT^AC
AflIII       1226  ACGCGT               A^C[GA][CT]GT
Alw44I        883  GTGCAC               G^TGCAC
AlwI          573  GGATC                GGATC(4/5)
Aor51HI       191  AGCGCT               AGC^GCT
ApaLI         883  GTGCAC               G^TGCAC
AscI          452  GGCGCGCC             GG^CGCGCC
AvaI         1013  CCCGAG               C^[CT]CG[GA]G
BalI          209  TGGCCA               TGG^CCA
BclI          110  TGATCA               T^GATCA
BlpI            9  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I        9  GCTCAGC              GC^T[ACGT]AGC
BsaAI         430  TACGTG               [CT]AC^GT[GA]
BsaI           58  GAGACC               GGTCTC(1/5)
BsaWI         706  ACCGGA               [AT]^CCGG[AT]
BsgI          357  CTGCAC               GTGCAG(16/14)
BsmAI          58  GAGAC                GTCTC(1/5)
BsoBI        1013  CCCGAG               C^[CT]CG[GA]G
BspMI         322  ACCTGC               ACCTGC(4/8)
BsrDI         158  GCAATG               GCAATG(2/0)
BstAPI        158  GCAATGCGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII           9  GCTCAGC              GC^T[ACGT]AGC
DdeI           10  CTCAG                C^T[ACGT]AG
DraIII        886  CACCGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI           88  GACGTGACCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          753  GAAGAG               CTCTTC(1/4)
Eco47III      191  AGCGCT               AGC^GCT
FbaI          110  TGATCA               T^GATCA
HinfI         769  GAGTC                G^A[ACGT]TC
Ksp632I       753  GAAGAG               CTCTTC(1/4)
MfeI         1155  CAATTG               C^AATTG
MluI         1226  ACGCGT               A^CGCGT
MluNI         209  TGGCCA               TGG^CCA
MscI          209  TGGCCA               TGG^CCA
MslI         1044  CATCATCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI         1155  CAATTG               C^AATTG
PleI          769  GAGTC                GAGTC(4/5)
PstI          199  CTGCAG               CTGCA^G
PvuII        1119  CAGCTG               CAG^CTG
RsaI          402  GTAC                 GT^AC
SfcI          199  CTGCAG               C^T[GA][CT]AG
SphI          556  GCATGC               GCATG^C


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AlwNI         399  CAGGTACTG            CAG[ACGT][ACGT][ACGT]^CTG
              780  CAGGAACTG           
BbeI          859  GGCGCC               GGCGC^C
             1144  GGCGCC              
BcnI          449  CCCGG                CC^[GC]GG
              531  CCCGG               
BpmI          786  CTGGAG               CTGGAG(16/14)
              868  CTGGAG              
BsrBI         540  CCGCTC               CCGCTC(-3/-3)
              710  GAGCGG              
BsrFI          45  ACCGGC               [GA]^CCGG[CT]
               61  ACCGGC              
BssHII        453  GCGCGC               G^CGCGC
              664  GCGCGC              
BstEII        237  GGTCACC              G^GT[ACGT]ACC
              318  GGTAACC             
BstPI         237  GGTCACC              G^GT[ACGT]ACC
              318  GGTAACC             
Cfr10I         45  ACCGGC               [GA]^CCGG[CT]
               61  ACCGGC              
DsaI          272  CCATGG               C^C[GA][CT]GG
              723  CCATGG              
EaeI          209  TGGCCA               [CT]^GGCC[GA]
              275  TGGCCG              
EcoO65I       237  GGTCACC              G^GT[ACGT]ACC
              318  GGTAACC             
KasI          859  GGCGCC               G^GCGCC
             1144  GGCGCC              
NarI          859  GGCGCC               GG^CGCC
             1144  GGCGCC              
NciI          449  CCCGG                CC^[GC]GG
              531  CCCGG               
NcoI          272  CCATGG               C^CATGG
              723  CCATGG              
NspI          556  GCATGC               [GA]CATG^[CT]
             1127  ACATGC              
SfoI          859  GGCGCC               GGC^GCC
             1144  GGCGCC              
Tsp509I       214  AATT                 ^AATT
             1156  AATT                
TspRI         308  CACTG                CA[GC]TG[ACGT][ACGT]^
             1028  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   66 enzymes) #
#########################################

AccII       AciI        AcyI        AluI        AspHI       
AvaII       BanI        BbsI        BbvI        BmyI        
BpuAI       BsaHI       BsaJI       BsiHKAI     BsiYI       
BslI        Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       BstUI       BstXI       Cac8I       CfoI        
Cfr13I      DpnII       EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HpaII       
HphI        ItaI        MaeII       MaeIII      MboI        
MboII       MnlI        MseI        MspA1I      MspI        
MvaI        MvnI        MwoI        NdeII       NlaIII      
NlaIV       Sau3AI      Sau96I      ScrFI       SfaNI       
StyI        TaqI        Tru9I       TseI        Tsp45I      
TthHB8I     
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