Restriction map for pF1KB9850
 (Circular) (Six-base) MAPSORT of: pF1KB9850.seq  Check: 8086  from: 1  to: 4244



pF1KB9850  4244 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:32  ..

AccI GT'mk_AC

Cuts at:   1282    1282 
   Size:       4244
 
Acc65I G'GTAC_C

Cuts at:   1260    1260 
   Size:       4244
 
AclI AA'CG_TT

Cuts at:   4084    4084 
   Size:       4244
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   2069    2501    3248    3516    2069 
   Size:        432     747     268    2797

  Fragments arranged by size: 

                2797     747     432     268
 
AfeI AGC'GCT

Cuts at:    820     820 
   Size:       4244
 
AflIII A'CryG_T

Cuts at:   1596    2700    3677    1596 
   Size:       1104     977    2163

  Fragments arranged by size: 

                2163    1104     977
 
AgeI A'CCGG_T

Cuts at:   2632    2632 
   Size:       4244
 
AlwNI CAG_nnn'CTG

Cuts at:    518     850    1549    3116     518 
   Size:        332     699    1567    1646

  Fragments arranged by size: 

                1646    1567     699     332
 
ApaLI G'TGCA_C

Cuts at:   3014    3014 
   Size:       4244
 
ApoI r'AATT_y

Cuts at:   1248    3597    3853    1248 
   Size:       2349     256    1639

  Fragments arranged by size: 

                2349    1639     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4244
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4244
 
AvaI C'yCGr_G

Cuts at:    989    1264     989 
   Size:        275    3969
 
BamHI G'GATC_C

Cuts at:    195    1103    1269     195 
   Size:        908     166    3170

  Fragments arranged by size: 

                3170     908     166
 
BanI G'GyrC_C

Cuts at:   1260    1674    1922    1957    1260 
   Size:        414     248      35    3547

  Fragments arranged by size: 

                3547     414     248      35
 
BanII G_rGCy'C

Cuts at:   1258    2288    1258 
   Size:       1030    3214
 
BbeI G_GCGC'C

Cuts at:   1678    1926    1678 
   Size:        248    3996
 
BciVI GTATCCnnnnn_n'

Cuts at:   2142    2903    4244    2142 
   Size:        761    1341    2142

  Fragments arranged by size: 

                2142    1341     761
 
BclI T'GATC_A

Cuts at:   1764    1764 
   Size:       4244
 
BglII A'GATC_T

Cuts at:    221     689    1759    3796     221 
   Size:        468    1070    2037     669

  Fragments arranged by size: 

                2037    1070     669     468
 
BlpI GC'TnA_GC

Cuts at:   1356    1356 
   Size:       4244
 
Bme1580I G_kGCm'C

Cuts at:    899     963    1869    1962    3018     899 
   Size:         64     906      93    1056    2125

  Fragments arranged by size: 

                2125    1056     906      93      64
 
BmrI ACTGGGnnnn_n'

Cuts at:    528    1456    1537    1872    4016     528 
   Size:        928      81     335    2144     756

  Fragments arranged by size: 

                2144     928     756     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    915    2611     915 
   Size:       1696    2548
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    193     864     193 
   Size:        671    3573
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    202    1420    2299    2791    3089     202 
   Size:       1218     879     492     298    1357

  Fragments arranged by size: 

                1357    1218     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3948    3948 
   Size:       4244
 
BsaAI yAC'GTr

Cuts at:    467     936    1597     467 
   Size:        469     661    3114

  Fragments arranged by size: 

                3114     661     469
 
BsaBI GATnn'nnATC

Cuts at:      6    1758    3795       6 
   Size:       1752    2037     455

  Fragments arranged by size: 

                2037    1752     455
 
BsaHI Gr'CG_yC

Cuts at:   1675    1923    2625    4121    1675 
   Size:        248     702    1496    1798

  Fragments arranged by size: 

                1798    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:   1226    1433    1954    2632    2906    3053    3464    1226 
   Size:        207     521     678     274     147     411    2006

  Fragments arranged by size: 

                2006     678     521     411     274     207     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    646     676     646 
   Size:         30    4214
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    670     670 
   Size:       4244
 
BseYI C'CCAG_C

Cuts at:   1669    2427    3004    3500    1669 
   Size:        758     577     496    2413

  Fragments arranged by size: 

                2413     758     577     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    456     456 
   Size:       4244
 
BsiEI CG_ry'CG

Cuts at:    104    1786    1832    3040     104 
   Size:       1682      46    1208    1308

  Fragments arranged by size: 

                1682    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:   1258    2036    2226    3018    1258 
   Size:        778     190     792    2484

  Fragments arranged by size: 

                2484     792     778     190
 
BsmI GAATG_Cn'

Cuts at:    174     174 
   Size:       4244
 
Bsp1286I G_dGCh'C

Cuts at:    899     963    1258    1869    1962    2036    2226    2288    3018 
   Size:         64     295     611      93      74     190      62     730

Cuts at:   3018     899 
   Size:       2125

  Fragments arranged by size: 

                2125     730     611     295     190      93      74      64
                  62
 
BspEI T'CCGG_A

Cuts at:   1226    3464    1226 
   Size:       2238    2006
 
BspHI T'CATG_A

Cuts at:   3420    3420 
   Size:       4244
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1294    1508    1810    2191    1294 
   Size:        214     302     381    3347

  Fragments arranged by size: 

                3347     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50     627    2536    2590    4074    4239      50 
   Size:        577    1909      54    1484     165      55

  Fragments arranged by size: 

                1909    1484     577     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    500    2156     500 
   Size:       1656    2588
 
BsrFI r'CCGG_y

Cuts at:   2242    2423    2632    2242 
   Size:        181     209    3854

  Fragments arranged by size: 

                3854     209     181
 
BssSI C'ACGA_G

Cuts at:   2515    2873    2515 
   Size:        358    3886
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1621    1621 
   Size:       4244
 
BstBI TT'CG_AA

Cuts at:   2605    2605 
   Size:       4244
 
BstYI r'GATC_y

Cuts at:      1     160     195     221     689     764    1103    1269    1736 
   Size:        159      35      26     468      75     339     166     467

Cuts at:   1736    1759    2094    2340    3352    3438    3450    3796       1 
   Size:         23     335     246    1012      86      12     346     449

  Fragments arranged by size: 

                1012     468     467     449     346     339     335     246
                 166     159      86      75      35      26      23      12
            
 
BtgI C'CryG_G

Cuts at:    233     827     233 
   Size:        594    3650
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    378    2194    2375    2538     378 
   Size:       1816     181     163    2084

  Fragments arranged by size: 

                2084    1816     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1487    1487 
   Size:       4244
 
DraI TTT'AAA

Cuts at:   1148    1243    1148 
   Size:         95    4149
 
DrdI GACnn_nn'nnGTC

Cuts at:   1950    2808    1950 
   Size:        858    3386
 
EaeI y'GGCC_r

Cuts at:    257     347     426     564     572     759    1829    2003    2394 
   Size:         90      79     138       8     187    1070     174     391

Cuts at:   2394    2421    3766     257 
   Size:         27    1345     735

  Fragments arranged by size: 

                1345    1070     735     391     187     174     138      90
                  79      27       8
 
EagI C'GGCC_G

Cuts at:   1829    1829 
   Size:       4244
 
EarI CTCTTCn'nnn_

Cuts at:    634    1184    2267    2477     634 
   Size:        550    1083     210    2401

  Fragments arranged by size: 

                2401    1083     550     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2452    2762    2908    4054    2452 
   Size:        310     146    1146    2642

  Fragments arranged by size: 

                2642    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   1256    1256 
   Size:       4244
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    193     864    2069    2501    3248    3516     193 
   Size:        671    1205     432     747     268     921

  Fragments arranged by size: 

                1205     921     747     671     432     268
 
EcoNI CCTnn'n_nnAGG

Cuts at:    142     889     142 
   Size:        747    3497
 
EcoO109I rG'GnC_Cy

Cuts at:    203     719     857    1031    1383     203 
   Size:        516     138     174     352    3064

  Fragments arranged by size: 

                3064     516     352     174     138
 
EcoRI G'AATT_C

Cuts at:   1248    1248 
   Size:       4244
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2263    2263 
   Size:       4244
 
FspI TGC'GCA

Cuts at:   2025    2025 
   Size:       4244
 
HaeII r_GCGC'y

Cuts at:    217     822    1117    1678    1926    2948    3927     217 
   Size:        605     295     561     248    1022     979     534

  Fragments arranged by size: 

                1022     979     605     561     534     295     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    646     678    2086    2116    2118    2148     646 
   Size:         32    1408      30       2      30    2742

  Fragments arranged by size: 

                2742    1408      32      30      30       2
 
HincII GTy'rAC

Cuts at:   1283    1283 
   Size:       4244
 
HindIII A'AGCT_T

Cuts at:    946    1202     946 
   Size:        256    3988
 
KasI G'GCGC_C

Cuts at:   1674    1922    1674 
   Size:        248    3996
 
KpnI G_GTAC'C

Cuts at:   1264    1264 
   Size:       4244
 
MluI A'CGCG_T

Cuts at:   3677    3677 
   Size:       4244
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    179     644    2915    3099     179 
   Size:        465    2271     184    1324

  Fragments arranged by size: 

                2271    1324     465     184
 
MscI TGG'CCA

Cuts at:    259     349     428     566    2005     259 
   Size:         90      79     138    1439    2498

  Fragments arranged by size: 

                2498    1439     138      90      79
 
MslI CAynn'nnrTG

Cuts at:    494    2233    2360     494 
   Size:       1739     127    2378

  Fragments arranged by size: 

                2378    1739     127
 
MspA1I CmG'CkG

Cuts at:   1355    1669    2029    3042    3287    1355 
   Size:        314     360    1013     245    2312

  Fragments arranged by size: 

                2312    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   2425    2425 
   Size:       4244
 
NarI GG'CG_CC

Cuts at:   1675    1923    1675 
   Size:        248    3996
 
NgoMIV G'CCGG_C

Cuts at:   2423    2423 
   Size:       4244
 
NspI r_CATG'y

Cuts at:   1297    1797    2704    1297 
   Size:        500     907    2837

  Fragments arranged by size: 

                2837     907     500
 
PciI A'CATG_T

Cuts at:   2700    2700 
   Size:       4244
 
PflMI CCAn_nnn'nTGG

Cuts at:    593     593 
   Size:       4244
 
PmeI GTTT'AAAC

Cuts at:   1243    1243 
   Size:       4244
 
PmlI CAC'GTG

Cuts at:    467     936     467 
   Size:        469    3775
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3383    3415    3383 
   Size:         32    4212
 
PpuMI rG'GwC_Cy

Cuts at:    203     857     203 
   Size:        654    3590
 
PstI C_TGCA'G

Cuts at:   1291    1976    1291 
   Size:        685    3559
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4244
 
PvuII CAG'CTG

Cuts at:   1669    2029    1669 
   Size:        360    3884
 
RsrII CG'GwC_CG

Cuts at:   2439    2439 
   Size:       4244
 
SacI G_AGCT'C

Cuts at:   1258    1258 
   Size:       4244
 
SalI G'TCGA_C

Cuts at:   1281    1281 
   Size:       4244
 
SapI GCTCTTCn'nnn_

Cuts at:    634    2267    2477     634 
   Size:       1633     210    2401

  Fragments arranged by size: 

                2401    1633     210
 
SbfI CC_TGCA'GG

Cuts at:   1291    1291 
   Size:       4244
 
ScaI AGT'ACT

Cuts at:   3761    3761 
   Size:       4244
 
SfcI C'TryA_G

Cuts at:     33    1287    1972    2965    3156      33 
   Size:       1254     685     993     191    1121

  Fragments arranged by size: 

                1254    1121     993     685     191
 
SfoI GGC'GCC

Cuts at:   1676    1924    1676 
   Size:        248    3996
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4244
 
SmaI CCC'GGG

Cuts at:   1266    1266 
   Size:       4244
 
SmlI C'TyrA_G

Cuts at:    217     989    1399    2314    2806    3068     217 
   Size:        772     410     915     492     262    1393

  Fragments arranged by size: 

                1393     915     772     492     410     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4244
 
SphI G_CATG'C

Cuts at:   1297    1797    1297 
   Size:        500    3744
 
StuI AGG'CCT

Cuts at:    137     151    1234     137 
   Size:         14    1083    3147

  Fragments arranged by size: 

                3147    1083      14
 
StyI C'CwwG_G

Cuts at:    644    1378    1732     644 
   Size:        734     354    3156

  Fragments arranged by size: 

                3156     734     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    424    1827    1960    2419    2628     424 
   Size:       1403     133     459     209    2040

  Fragments arranged by size: 

                2040    1403     459     209     133
 
TatI w'GTAC_w

Cuts at:    282    3759     282 
   Size:       3477     767
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3761    3793    3761 
   Size:         32    4212
 
Tth111I GACn'n_nGTC

Cuts at:   2041    2041 
   Size:       4244
 
XbaI T'CTAG_A

Cuts at:   1275    1275 
   Size:       4244
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    360    1011    1739     360 
   Size:        651     728    2865

  Fragments arranged by size: 

                2865     728     651
 
XhoI C'TCGA_G

Cuts at:    989     989 
   Size:       4244
 
XmaI C'CCGG_G

Cuts at:   1264    1264 
   Size:       4244
 
XmnI GAAnn'nnTTC

Cuts at:    137     820    1193     137 
   Size:        683     373    3188

  Fragments arranged by size: 

                3188     683     373
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI     AfeI   AflIII     AgeI    AlwNI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI    BciVI     BclI    BglII     BlpI Bme1580I     BmrI     BplI
     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI
    BstYI     BtgI    BtgZI     BtsI     DraI     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI
     FspI    HaeII    Hin4I   HincII  HindIII     KasI     KpnI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI   NgoMIV     NspI
     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI     PstI     PvuI
    PvuII    RsrII     SacI     SalI     SapI     SbfI     ScaI     SfcI
     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII    AflII     AhdI     AleI     AlfI     AloI     ApaI
     AscI    AvrII     BaeI     BbsI    BbvCI     BcgI    BfrBI     BglI
    BmgBI     BmtI   Bpu10I    BsiWI    BsmBI    BsrGI   BssHII   BstEII
    BstXI  BstZ17I   Bsu36I     ClaI    CspCI   DraIII    EcoRV     FseI
    FspAI     HpaI     MfeI     NcoI     NdeI     NheI     NotI     NruI
     NsiI     PacI     PasI     PfoI    PshAI     PsiI   PspOMI    PspXI
     PsrI    SacII    SanDI    SexAI     SfiI    SgrAI    SnaBI     SrfI
     SspI     SwaI     ZraI
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