Restriction map for pF1KB9847
##################################
# NO CUT ENZYMES (  138 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AflII       AflIII      AgeI        AhdI        Alw44I      
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp718I     AspEI       AspI        
AvaI        AviII       AvrII       BamHI       BanII       
BbrPI       BbvCI       BcgI        BciVI       BclI        
BcnI        BfaI        BfrI        BglII       BlnI        
BlpI        Bpu1102I    BsaAI       BsaBI       BsaWI       
BseAI       BsgI        BsiEI       BsiWI       BsmBI       
BsmI        BsoBI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrBI       BsrGI       BssHII      
Bst1107I    BstAPI      BstBI       BstZ17I     CelII       
ClaI        CpoI        DraI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      EcoNI       EcoRI       
EcoRV       EcoT22I     FauI        FbaI        FseI        
FspI        HindIII     HpaI        KpnI        KspI        
MaeI        MamI        MfeI        MluI        MroI        
MseI        MunI        NaeI        NciI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PshBI       
Psp1406I    PvuI        PvuII       RcaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
SexAI       SfiI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        SwaI        TaqI        Tru9I       
Tsp509I     Tth111I     TthHB8I     Van91I      XbaI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AccI          298  GTCTAC               GT^[AC][GT]AC
AccII         736  CGCG                 CG^CG
AcyI          716  GGCGCC               G[GA]^CG[CT]C
AlwI          320  GGATC                GGATC(4/5)
AlwNI         283  CAGCCGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI         1095  CCTGAGG              CC^T[ACGT]AGG
Asp700I       543  GAATGTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbeI          716  GGCGCC               GGCGC^C
BglI          241  GCCAACCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI         716  GGCGCC               G[GA]^CG[CT]C
BsmFI        1092  GTCCC                GGGAC(10/14)
BsrFI         713  ACCGGC               [GA]^CCGG[CT]
BssSI         400  CTCGTG               CACGAG(-5/-1)
BstEII        172  GGTAACC              G^GT[ACGT]ACC
BstPI         172  GGTAACC              G^GT[ACGT]ACC
BstUI         736  CGCG                 CG^CG
BstYI         320  GGATCT               [GA]^GATC[CT]
Bsu36I       1095  CCTGAGG              CC^T[ACGT]AGG
Cfr10I        713  ACCGGC               [GA]^CCGG[CT]
DraIII         44  CACAAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          115  GACGTGATGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          658  CTCTTC               CTCTTC(1/4)
Eco81I       1095  CCTGAGG              CC^T[ACGT]AGG
EcoO65I       172  GGTAACC              G^GT[ACGT]ACC
HapII         714  CCGG                 C^CGG
HgaI          947  GCGTC                GACGC(5/10)
Hin1I         716  GGCGCC               G[GA]^CG[CT]C
HincII        949  GTCAAC               GT[CT]^[GA]AC
HindII        949  GTCAAC               GT[CT]^[GA]AC
HpaII         714  CCGG                 C^CGG
KasI          716  GGCGCC               G^GCGCC
Ksp632I       658  CTCTTC               CTCTTC(1/4)
MflI          320  GGATCT               [GA]^GATC[CT]
MspA1I        286  CCGCTG               C[AC]G^C[GT]G
MspI          714  CCGG                 C^CGG
MvnI          736  CGCG                 CG^CG
NarI          716  GGCGCC               GG^CGCC
NcoI          932  CCATGG               C^CATGG
PshAI         291  GACCGCCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI          721  CTGCAG               CTGCA^G
ScaI          665  AGTACT               AGT^ACT
SfoI          716  GGCGCC               GGC^GCC
StuI          464  AGGCCT               AGG^CCT
TfiI          106  GATTC                G^A[AT]TC
XcmI          527  CCAACAGCATCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         320  GGATCT               [GA]^GATC[CT]
XmnI          543  GAATGTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AspHI         481  GTGCTC               G[AT]GC[AT]^C
              826  GTGCTC              
BalI          524  TGGCCA               TGG^CCA
              613  TGGCCA              
BbvI           27  GCTGC                GCAGC(8/12)
              834  GCTGC               
BsaI          331  GAGACC               GGTCTC(1/5)
              378  GGTCTC              
BsiHKAI       481  GTGCTC               G[AT]GC[AT]^C
              826  GTGCTC              
BsrDI          97  CATTGC               GCAATG(2/0)
              495  CATTGC              
BstXI         436  CCAACAGGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              566  CCAAGGCTCTGG        
DpnII         321  GATC                 ^GATC
              996  GATC                
EaeI          524  TGGCCA               [CT]^GGCC[GA]
              613  TGGCCA              
EcoT14I       566  CCAAGG               C^C[AT][AT]GG
              932  CCATGG              
HaeII         716  GGCGCC               [GA]GCGC^[CT]
             1027  AGCGCC              
MaeII         116  ACGT                 A^CGT
              221  ACGT                
MboI          321  GATC                 ^GATC
              996  GATC                
MluNI         524  TGGCCA               TGG^CCA
              613  TGGCCA              
MscI          524  TGGCCA               TGG^CCA
              613  TGGCCA              
MslI          303  CACCATCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              366  CATGTCGGTG          
NdeII         321  GATC                 ^GATC
              996  GATC                
NspI          365  GCATGT               [GA]CATG^[CT]
              843  GCATGT              
PleI          607  GAGTC                GAGTC(4/5)
             1042  GAGTC               
PpuMI         164  GGGTCCT              [GA]G^G[AT]CC[CT]
             1090  GGGTCCC             
Sau3AI        321  GATC                 ^GATC
              996  GATC                
StyI          566  CCAAGG               C^C[AT][AT]GG
              932  CCATGG              
TseI           27  GCTGC                G^C[AT]GC
              834  GCTGC               


#########################################
# 3 OR MORE CUT ENZYMES (   50 enzymes) #
#########################################

AciI        AfaI        AluI        AvaII       BanI        
BbsI        BmyI        BpmI        BpuAI       BsaJI       
BseRI       BsiYI       BslI        BsmAI       Bsp1286I    
BspMI       BsrI        BssKI       BstF5I      BstNI       
Cac8I       CfoI        Cfr13I      DdeI        DraII       
DsaI        EcoO109I    EcoRII      Fnu4HI      FokI        
HaeIII      HhaI        HinP1I      HinfI       HphI        
ItaI        MaeIII      MboII       MnlI        MvaI        
MwoI        NlaIII      NlaIV       RsaI        Sau96I      
ScrFI       SfaNI       SfcI        Tsp45I      TspRI       
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