Restriction map for
pF1KB9847
(Circular) (Six-base) MAPSORT of: pF1KB9847.seq Check: 7226 from: 1 to: 4238
pF1KB9847 4238 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1060 1060
Size: 4238
AccI GT'mk_AC
Cuts at: 407 1276 407
Size: 869 3369
Acc65I G'GTAC_C
Cuts at: 1254 1254
Size: 4238
AclI AA'CG_TT
Cuts at: 4078 4078
Size: 4238
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2063 2495 3242 3510 2063
Size: 432 747 268 2791
Fragments arranged by size:
2791 747 432 268
AflIII A'CryG_T
Cuts at: 1590 2694 3671 1590
Size: 1104 977 2157
Fragments arranged by size:
2157 1104 977
AgeI A'CCGG_T
Cuts at: 2626 2626
Size: 4238
AlwNI CAG_nnn'CTG
Cuts at: 396 1543 3110 396
Size: 1147 1567 1524
Fragments arranged by size:
1567 1524 1147
ApaLI G'TGCA_C
Cuts at: 3008 3008
Size: 4238
ApoI r'AATT_y
Cuts at: 1242 3591 3847 1242
Size: 2349 256 1633
Fragments arranged by size:
2349 1633 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4238
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4238
AvaI C'yCGr_G
Cuts at: 1258 1258
Size: 4238
BamHI G'GATC_C
Cuts at: 1263 1263
Size: 4238
BanI G'GyrC_C
Cuts at: 181 824 859 977 1254 1668 1916 1951 181
Size: 643 35 118 277 414 248 35 2468
Fragments arranged by size:
2468 643 414 277 248 118 35 35
BanII G_rGCy'C
Cuts at: 1252 2282 1252
Size: 1030 3208
BbeI G_GCGC'C
Cuts at: 828 1672 1920 828
Size: 844 248 3146
Fragments arranged by size:
3146 844 248
BbsI GAAGACnn'nnnn_
Cuts at: 225 648 977 225
Size: 423 329 3486
Fragments arranged by size:
3486 423 329
BciVI GTATCCnnnnn_n'
Cuts at: 2136 2897 4238 2136
Size: 761 1341 2136
Fragments arranged by size:
2136 1341 761
BclI T'GATC_A
Cuts at: 1758 1758
Size: 4238
BglI GCCn_nnn'nGGC
Cuts at: 355 355
Size: 4238
BglII A'GATC_T
Cuts at: 1753 3790 1753
Size: 2037 2201
BlpI GC'TnA_GC
Cuts at: 1350 1350
Size: 4238
Bme1580I G_kGCm'C
Cuts at: 184 862 884 1863 1956 3012 184
Size: 678 22 979 93 1056 1410
Fragments arranged by size:
1410 1056 979 678 93 22
BmgBI CAC'GTC
Cuts at: 225 225
Size: 4238
BmrI ACTGGGnnnn_n'
Cuts at: 796 1450 1531 1866 4010 796
Size: 654 81 335 2144 1024
Fragments arranged by size:
2144 1024 654 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 460 2605 460
Size: 2145 2093
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 538 667 703 1165 538
Size: 129 36 462 3611
Fragments arranged by size:
3611 462 129 36
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1414 2293 2785 3083 1414
Size: 879 492 298 2569
Fragments arranged by size:
2569 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 433 492 3942 433
Size: 59 3450 729
Fragments arranged by size:
3450 729 59
BsaAI yAC'GTr
Cuts at: 1591 1591
Size: 4238
BsaBI GATnn'nnATC
Cuts at: 6 1752 3789 6
Size: 1746 2037 455
Fragments arranged by size:
2037 1746 455
BsaHI Gr'CG_yC
Cuts at: 825 1669 1917 2619 4115 825
Size: 844 248 702 1496 948
Fragments arranged by size:
1496 948 844 702 248
BsaWI w'CCGG_w
Cuts at: 1427 1948 2626 2900 3047 3458 1427
Size: 521 678 274 147 411 2207
Fragments arranged by size:
2207 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 373 754 1162 373
Size: 381 408 3449
Fragments arranged by size:
3449 408 381
BseYI C'CCAG_C
Cuts at: 559 877 1663 2421 2998 3494 559
Size: 318 786 758 577 496 1303
Fragments arranged by size:
1303 786 758 577 496 318
BsiEI CG_ry'CG
Cuts at: 104 1780 1826 3034 104
Size: 1676 46 1208 1308
Fragments arranged by size:
1676 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 593 938 1252 2030 2220 3012 593
Size: 345 314 778 190 792 1819
Fragments arranged by size:
1819 792 778 345 314 190
Bsp1286I G_dGCh'C
Cuts at: 184 593 862 884 938 1252 1863 1956 2030
Size: 409 269 22 54 314 611 93 74
Cuts at: 2030 2220 2282 3012 184
Size: 190 62 730 1410
Fragments arranged by size:
1410 730 611 409 314 269 190 93
74 62 54 22
BspEI T'CCGG_A
Cuts at: 3458 3458
Size: 4238
BspHI T'CATG_A
Cuts at: 3414 3414
Size: 4238
BspMI ACCTGCnnnn'nnnn_
Cuts at: 347 882 1060 1208 1288 1502 1804 2185 347
Size: 535 178 148 80 214 302 381 2400
Fragments arranged by size:
2400 535 381 302 214 178 148 80
BsrBI CCG'CTC
Cuts at: 50 2530 2584 4068 4233 50
Size: 2480 54 1484 165 55
Fragments arranged by size:
2480 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 204 602 2150 204
Size: 398 1548 2292
Fragments arranged by size:
2292 1548 398
BsrFI r'CCGG_y
Cuts at: 821 2236 2417 2626 821
Size: 1415 181 209 2433
Fragments arranged by size:
2433 1415 209 181
BssSI C'ACGA_G
Cuts at: 508 2509 2867 508
Size: 2001 358 1879
Fragments arranged by size:
2001 1879 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1615 1615
Size: 4238
BstBI TT'CG_AA
Cuts at: 2599 2599
Size: 4238
BstEII G'GTnAC_C
Cuts at: 280 280
Size: 4238
BstXI CCAn_nnnn'nTGG
Cuts at: 551 681 551
Size: 130 4108
BstYI r'GATC_y
Cuts at: 1 428 1263 1730 1753 2088 2334 3346 3432
Size: 427 835 467 23 335 246 1012 86
Cuts at: 3432 3444 3790 1
Size: 12 346 449
Fragments arranged by size:
1012 835 467 449 427 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1204 1204
Size: 4238
BtgI C'CryG_G
Cuts at: 218 1002 1040 218
Size: 784 38 3416
Fragments arranged by size:
3416 784 38
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2188 2369 2532 2188
Size: 181 163 3894
Fragments arranged by size:
3894 181 163
BtsI GCAGTG_nn'
Cuts at: 1481 1481
Size: 4238
DraI TTT'AAA
Cuts at: 1237 1237
Size: 4238
DraIII CAC_nnn'GTG
Cuts at: 157 157
Size: 4238
DrdI GACnn_nn'nnGTC
Cuts at: 229 1944 2802 229
Size: 1715 858 1665
Fragments arranged by size:
1715 1665 858
EaeI y'GGCC_r
Cuts at: 632 721 1823 1997 2388 2415 3760 632
Size: 89 1102 174 391 27 1345 1110
Fragments arranged by size:
1345 1110 1102 391 174 89 27
EagI C'GGCC_G
Cuts at: 1823 1823
Size: 4238
EarI CTCTTCn'nnn_
Cuts at: 772 2261 2471 772
Size: 1489 210 2539
Fragments arranged by size:
2539 1489 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 709 2446 2756 2902 4048 709
Size: 1737 310 146 1146 899
Fragments arranged by size:
1737 1146 899 310 146
EcoICRI GAG'CTC
Cuts at: 1250 1250
Size: 4238
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 538 667 703 1165 2063 2495 3242 3510 538
Size: 129 36 462 898 432 747 268 1266
Fragments arranged by size:
1266 898 747 462 432 268 129 36
EcoO109I rG'GnC_Cy
Cuts at: 273 1110 1199 1377 273
Size: 837 89 178 3134
Fragments arranged by size:
3134 837 178 89
EcoRI G'AATT_C
Cuts at: 1242 1242
Size: 4238
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2257 2257
Size: 4238
FspI TGC'GCA
Cuts at: 2019 2019
Size: 4238
HaeII r_GCGC'y
Cuts at: 828 1139 1672 1920 2942 3921 828
Size: 311 533 248 1022 979 1145
Fragments arranged by size:
1145 1022 979 533 311 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2080 2110 2112 2142 2080
Size: 30 2 30 4176
Fragments arranged by size:
4176 30 30 2
HincII GTy'rAC
Cuts at: 1059 1277 1059
Size: 218 4020
KasI G'GCGC_C
Cuts at: 824 1668 1916 824
Size: 844 248 3146
Fragments arranged by size:
3146 844 248
KpnI G_GTAC'C
Cuts at: 1258 1258
Size: 4238
MluI A'CGCG_T
Cuts at: 3671 3671
Size: 4238
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1133 2909 3093 1133
Size: 1776 184 2278
Fragments arranged by size:
2278 1776 184
MscI TGG'CCA
Cuts at: 634 723 1999 634
Size: 89 1276 2873
Fragments arranged by size:
2873 1276 89
MslI CAynn'nnrTG
Cuts at: 415 478 2227 2354 415
Size: 63 1749 127 2299
Fragments arranged by size:
2299 1749 127 63
MspA1I CmG'CkG
Cuts at: 396 1349 1663 2023 3036 3281 396
Size: 953 314 360 1013 245 1353
Fragments arranged by size:
1353 1013 953 360 314 245
NaeI GCC'GGC
Cuts at: 2419 2419
Size: 4238
NarI GG'CG_CC
Cuts at: 825 1669 1917 825
Size: 844 248 3146
Fragments arranged by size:
3146 844 248
NcoI C'CATG_G
Cuts at: 1040 1040
Size: 4238
NgoMIV G'CCGG_C
Cuts at: 2417 2417
Size: 4238
NspI r_CATG'y
Cuts at: 477 955 1291 1791 2698 477
Size: 478 336 500 907 2017
Fragments arranged by size:
2017 907 500 478 336
PasI CC'CwG_GG
Cuts at: 177 177
Size: 4238
PciI A'CATG_T
Cuts at: 2694 2694
Size: 4238
PfoI T'CCnGG_A
Cuts at: 644 644
Size: 4238
PmeI GTTT'AAAC
Cuts at: 1237 1237
Size: 4238
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3377 3409 3377
Size: 32 4206
PpuMI rG'GwC_Cy
Cuts at: 273 1199 273
Size: 926 3312
PshAI GACnn'nnGTC
Cuts at: 403 403
Size: 4238
PstI C_TGCA'G
Cuts at: 833 1285 1970 833
Size: 452 685 3101
Fragments arranged by size:
3101 685 452
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4238
PvuII CAG'CTG
Cuts at: 1663 2023 1663
Size: 360 3878
RsrII CG'GwC_CG
Cuts at: 2433 2433
Size: 4238
SacI G_AGCT'C
Cuts at: 1252 1252
Size: 4238
SalI G'TCGA_C
Cuts at: 1275 1275
Size: 4238
SanDI GG'GwC_CC
Cuts at: 1199 1199
Size: 4238
SapI GCTCTTCn'nnn_
Cuts at: 2261 2471 2261
Size: 210 4028
SbfI CC_TGCA'GG
Cuts at: 1285 1285
Size: 4238
ScaI AGT'ACT
Cuts at: 775 3755 775
Size: 2980 1258
SfcI C'TryA_G
Cuts at: 33 249 829 864 1281 1966 2959 3150 33
Size: 216 580 35 417 685 993 191 1121
Fragments arranged by size:
1121 993 685 580 417 216 191 35
SfoI GGC'GCC
Cuts at: 826 1670 1918 826
Size: 844 248 3146
Fragments arranged by size:
3146 844 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4238
SmaI CCC'GGG
Cuts at: 1260 1260
Size: 4238
SmlI C'TyrA_G
Cuts at: 1393 2308 2800 3062 1393
Size: 915 492 262 2569
Fragments arranged by size:
2569 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4238
SphI G_CATG'C
Cuts at: 1291 1791 1291
Size: 500 3738
StuI AGG'CCT
Cuts at: 574 574
Size: 4238
StyI C'CwwG_G
Cuts at: 674 1040 1372 1726 674
Size: 366 332 354 3186
Fragments arranged by size:
3186 366 354 332
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1821 1954 2413 2622 1821
Size: 133 459 209 3437
Fragments arranged by size:
3437 459 209 133
TatI w'GTAC_w
Cuts at: 773 1176 3753 773
Size: 403 2577 1258
Fragments arranged by size:
2577 1258 403
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3755 3787 3755
Size: 32 4206
Tth111I GACn'n_nGTC
Cuts at: 2035 2035
Size: 4238
XbaI T'CTAG_A
Cuts at: 1269 1269
Size: 4238
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 642 1733 642
Size: 1091 3147
XmaI C'CCGG_G
Cuts at: 1258 1258
Size: 4238
XmnI GAAnn'nnTTC
Cuts at: 655 655
Size: 4238
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PfoI PmeI PpiI
PpuMI PshAI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BmtI Bpu10I
BsaXI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstZ17I
ClaI CspCI EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PflMI
PmlI PsiI PspOMI PspXI PsrI SacII SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI ZraI