Restriction map for pF1KB9847
 (Linear) (Six-base) MAPSORT of: KIBB9847.seq  Check: 9989  from: 1  to: 1125



KIBB9847.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:12  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     952    1125 
   Size:        952     173
 
AccI GT'mk_AC

Cuts at:      0     299    1125 
   Size:        299     826
 
AlwNI CAG_nnn'CTG

Cuts at:      0     288    1125 
   Size:        288     837
 
BanI G'GyrC_C

Cuts at:      0      73     716     751     869    1125 
   Size:         73     643      35     118     256

  Fragments arranged by size: 

                 643     256     118      73      35
 
BbeI G_GCGC'C

Cuts at:      0     720    1125 
   Size:        720     405
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     117     540     869    1125 
   Size:        117     423     329     256

  Fragments arranged by size: 

                 423     329     256     117
 
BglI GCCn_nnn'nGGC

Cuts at:      0     247    1125 
   Size:        247     878
 
Bme1580I G_kGCm'C

Cuts at:      0      76     754     776    1125 
   Size:         76     678      22     349

  Fragments arranged by size: 

                 678     349      76      22
 
BmgBI CAC'GTC

Cuts at:      0     117    1125 
   Size:        117    1008
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     688    1125 
   Size:        688     437
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     352    1125 
   Size:        352     773
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     430     559     595    1057    1125 
   Size:        430     129      36     462      68

  Fragments arranged by size: 

                 462     430     129      68      36
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     325     384    1125 
   Size:        325      59     741

  Fragments arranged by size: 

                 741     325      59
 
BsaHI Gr'CG_yC

Cuts at:      0     717    1125 
   Size:        717     408
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     265     646    1054    1125 
   Size:        265     381     408      71

  Fragments arranged by size: 

                 408     381     265      71
 
BseYI C'CCAG_C

Cuts at:      0     451     769    1125 
   Size:        451     318     356

  Fragments arranged by size: 

                 451     356     318
 
BsiHKAI G_wGCw'C

Cuts at:      0     485     830    1125 
   Size:        485     345     295

  Fragments arranged by size: 

                 485     345     295
 
Bsp1286I G_dGCh'C

Cuts at:      0      76     485     754     776     830    1125 
   Size:         76     409     269      22      54     295

  Fragments arranged by size: 

                 409     295     269      76      54      22
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     239     774     952    1100    1125 
   Size:        239     535     178     148      25

  Fragments arranged by size: 

                 535     239     178     148      25
 
BsrDI GCAATG_nn'

Cuts at:      0      96     494    1125 
   Size:         96     398     631

  Fragments arranged by size: 

                 631     398      96
 
BsrFI r'CCGG_y

Cuts at:      0     713    1125 
   Size:        713     412
 
BssSI C'ACGA_G

Cuts at:      0     400    1125 
   Size:        400     725
 
BstEII G'GTnAC_C

Cuts at:      0     172    1125 
   Size:        172     953
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     443     573    1125 
   Size:        443     130     552

  Fragments arranged by size: 

                 552     443     130
 
BstYI r'GATC_y

Cuts at:      0     320    1125 
   Size:        320     805
 
Bsu36I CC'TnA_GG

Cuts at:      0    1096    1125 
   Size:       1096      29
 
BtgI C'CryG_G

Cuts at:      0     110     894     932    1125 
   Size:        110     784      38     193

  Fragments arranged by size: 

                 784     193     110      38
 
DraIII CAC_nnn'GTG

Cuts at:      0      49    1125 
   Size:         49    1076
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     121    1125 
   Size:        121    1004
 
EaeI y'GGCC_r

Cuts at:      0     524     613    1125 
   Size:        524      89     512

  Fragments arranged by size: 

                 524     512      89
 
EarI CTCTTCn'nnn_

Cuts at:      0     664    1125 
   Size:        664     461
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     601    1125 
   Size:        601     524
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     430     559     595    1057    1125 
   Size:        430     129      36     462      68

  Fragments arranged by size: 

                 462     430     129      68      36
 
EcoO109I rG'GnC_Cy

Cuts at:      0     165    1002    1091    1125 
   Size:        165     837      89      34

  Fragments arranged by size: 

                 837     165      89      34
 
HaeII r_GCGC'y

Cuts at:      0     720    1031    1125 
   Size:        720     311      94

  Fragments arranged by size: 

                 720     311      94
 
HincII GTy'rAC

Cuts at:      0     951    1125 
   Size:        951     174
 
KasI G'GCGC_C

Cuts at:      0     716    1125 
   Size:        716     409
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1025    1125 
   Size:       1025     100
 
MscI TGG'CCA

Cuts at:      0     526     615    1125 
   Size:        526      89     510

  Fragments arranged by size: 

                 526     510      89
 
MslI CAynn'nnrTG

Cuts at:      0     307     370    1125 
   Size:        307      63     755

  Fragments arranged by size: 

                 755     307      63
 
MspA1I CmG'CkG

Cuts at:      0     288    1125 
   Size:        288     837
 
NarI GG'CG_CC

Cuts at:      0     717    1125 
   Size:        717     408
 
NcoI C'CATG_G

Cuts at:      0     932    1125 
   Size:        932     193
 
NspI r_CATG'y

Cuts at:      0     369     847    1125 
   Size:        369     478     278

  Fragments arranged by size: 

                 478     369     278
 
PasI CC'CwG_GG

Cuts at:      0      69    1125 
   Size:         69    1056
 
PfoI T'CCnGG_A

Cuts at:      0     536    1125 
   Size:        536     589
 
PpuMI rG'GwC_Cy

Cuts at:      0     165    1091    1125 
   Size:        165     926      34

  Fragments arranged by size: 

                 926     165      34
 
PshAI GACnn'nnGTC

Cuts at:      0     295    1125 
   Size:        295     830
 
PstI C_TGCA'G

Cuts at:      0     725    1125 
   Size:        725     400
 
SanDI GG'GwC_CC

Cuts at:      0    1091    1125 
   Size:       1091      34
 
ScaI AGT'ACT

Cuts at:      0     667    1125 
   Size:        667     458
 
SfcI C'TryA_G

Cuts at:      0     141     721     756    1125 
   Size:        141     580      35     369

  Fragments arranged by size: 

                 580     369     141      35
 
SfoI GGC'GCC

Cuts at:      0     718    1125 
   Size:        718     407
 
StuI AGG'CCT

Cuts at:      0     466    1125 
   Size:        466     659
 
StyI C'CwwG_G

Cuts at:      0     566     932    1125 
   Size:        566     366     193

  Fragments arranged by size: 

                 566     366     193
 
TatI w'GTAC_w

Cuts at:      0     665    1068    1125 
   Size:        665     403      57

  Fragments arranged by size: 

                 665     403      57
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     534    1125 
   Size:        534     591
 
XmnI GAAnn'nnTTC

Cuts at:      0     547    1125 
   Size:        547     578
 

 Enzymes that do cut:

     AarI     AccI    AlwNI     BanI     BbeI     BbsI     BglI Bme1580I
    BmgBI     BmrI     BplI     BpmI     BsaI    BsaHI    BseRI    BseYI
  BsiHKAI Bsp1286I    BspMI    BsrDI    BsrFI    BssSI   BstEII    BstXI
    BstYI   Bsu36I     BtgI   DraIII     DrdI     EaeI     EarI     EciI
  Eco57MI EcoO109I    HaeII   HincII     KasI     MmeI     MscI     MslI
   MspA1I     NarI     NcoI     NspI     PasI     PfoI    PpuMI    PshAI
     PstI    SanDI     ScaI     SfcI     SfoI     StuI     StyI     TatI
     XcmI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII    BbvCI
     BcgI    BciVI     BclI    BfrBI    BglII     BlpI     BmtI   Bpu10I
    BpuEI    BsaAI    BsaBI    BsaWI    BsaXI     BsgI    BsiEI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrGI   BssHII   BstAPI
    BstBI  BstZ17I    BtgZI     BtsI     ClaI    CspCI     DraI     EagI
  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    Hin4I  HindIII     HpaI     KpnI     MfeI     MluI     NaeI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PciI    PflMI
     PmeI     PmlI     PpiI     PsiI   PspOMI    PspXI     PsrI     PvuI
    PvuII    RsrII     SacI    SacII     SalI     SapI     SbfI    SexAI
     SfiI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     SwaI    TaqII     TstI  Tth111I     XbaI     XhoI
     XmaI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com