Restriction map for pF1KB9845
 (Circular) (Six-base) MAPSORT of: pF1KB9845.seq  Check: 4651  from: 1  to: 4232



pF1KB9845  4232 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:32  ..

AccI GT'mk_AC

Cuts at:    278    1270     278 
   Size:        992    3240
 
Acc65I G'GTAC_C

Cuts at:    679    1248     679 
   Size:        569    3663
 
AclI AA'CG_TT

Cuts at:   4072    4072 
   Size:       4232
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   2057    2489    3236    3504    2057 
   Size:        432     747     268    2785

  Fragments arranged by size: 

                2785     747     432     268
 
AflIII A'CryG_T

Cuts at:    374    1584    2688    3665     374 
   Size:       1210    1104     977     941

  Fragments arranged by size: 

                1210    1104     977     941
 
AgeI A'CCGG_T

Cuts at:    551    2620     551 
   Size:       2069    2163
 
AhdI GACnn_n'nnGTC

Cuts at:   1037    1037 
   Size:       4232
 
AleI CACnn'nnGTG

Cuts at:    772     772 
   Size:       4232
 
AlwNI CAG_nnn'CTG

Cuts at:   1537    3104    1537 
   Size:       1567    2665
 
ApaI G_GGCC'C

Cuts at:    966     966 
   Size:       4232
 
ApaLI G'TGCA_C

Cuts at:    765    3002     765 
   Size:       2237    1995
 
ApoI r'AATT_y

Cuts at:   1152    1236    3585    3841    1152 
   Size:         84    2349     256    1543

  Fragments arranged by size: 

                2349    1543     256      84
 
AseI AT'TA_AT

Cuts at:     20     823      20 
   Size:        803    3429
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4232
 
AvaI C'yCGr_G

Cuts at:   1115    1252    1115 
   Size:        137    4095
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:    546     579     663     671     696     704     546 
   Size:         33      84       8      25       8    4074

  Fragments arranged by size: 

                4074      84      33      25       8       8
 
BamHI G'GATC_C

Cuts at:   1257    1257 
   Size:       4232
 
BanI G'GyrC_C

Cuts at:    679    1248    1662    1910    1945     679 
   Size:        569     414     248      35    2966

  Fragments arranged by size: 

                2966     569     414     248      35
 
BanII G_rGCy'C

Cuts at:    966    1246    2276     966 
   Size:        280    1030    2922

  Fragments arranged by size: 

                2922    1030     280
 
BbeI G_GCGC'C

Cuts at:   1666    1914    1666 
   Size:        248    3984
 
BbsI GAAGACnn'nnnn_

Cuts at:    330    1178     330 
   Size:        848    3384
 
BciVI GTATCCnnnnn_n'

Cuts at:   2130    2891    4232    2130 
   Size:        761    1341    2130

  Fragments arranged by size: 

                2130    1341     761
 
BclI T'GATC_A

Cuts at:   1752    1752 
   Size:       4232
 
BfrBI ATG'CAT

Cuts at:    539     539 
   Size:       4232
 
BglI GCCn_nnn'nGGC

Cuts at:    160     160 
   Size:       4232
 
BglII A'GATC_T

Cuts at:    849    1747    3784     849 
   Size:        898    2037    1297

  Fragments arranged by size: 

                2037    1297     898
 
BlpI GC'TnA_GC

Cuts at:   1344    1344 
   Size:       4232
 
Bme1580I G_kGCm'C

Cuts at:    549     769     966    1857    1950    3006     549 
   Size:        220     197     891      93    1056    1775

  Fragments arranged by size: 

                1775    1056     891     220     197      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1444    1525    1860    4004    1444 
   Size:         81     335    2144    1672

  Fragments arranged by size: 

                2144    1672     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2599    2599 
   Size:       4232
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    375     375 
   Size:       4232
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    562     978    1408    2287    2779    3077     562 
   Size:        416     430     879     492     298    1717

  Fragments arranged by size: 

                1717     879     492     430     416     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3936    3936 
   Size:       4232
 
BsaAI yAC'GTr

Cuts at:    408    1022    1585     408 
   Size:        614     563    3055

  Fragments arranged by size: 

                3055     614     563
 
BsaBI GATnn'nnATC

Cuts at:      6     616    1746    3783       6 
   Size:        610    1130    2037     455

  Fragments arranged by size: 

                2037    1130     610     455
 
BsaHI Gr'CG_yC

Cuts at:   1663    1911    2613    4109    1663 
   Size:        248     702    1496    1786

  Fragments arranged by size: 

                1786    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:    551    1421    1942    2620    2894    3041    3452     551 
   Size:        870     521     678     274     147     411    1331

  Fragments arranged by size: 

                1331     870     678     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    207     854     885     207 
   Size:        647      31    3554

  Fragments arranged by size: 

                3554     647      31
 
BseYI C'CCAG_C

Cuts at:    984    1657    2415    2992    3488     984 
   Size:        673     758     577     496    1728

  Fragments arranged by size: 

                1728     758     673     577     496
 
BsiEI CG_ry'CG

Cuts at:    104     197     773    1774    1820    3028     104 
   Size:         93     576    1001      46    1208    1308

  Fragments arranged by size: 

                1308    1208    1001     576      93      46
 
BsiHKAI G_wGCw'C

Cuts at:    769    1246    2024    2214    3006     769 
   Size:        477     778     190     792    1995

  Fragments arranged by size: 

                1995     792     778     477     190
 
BsmI GAATG_Cn'

Cuts at:    612     612 
   Size:       4232
 
BsmBI CGTCTCn'nnnn_

Cuts at:    219     219 
   Size:       4232
 
Bsp1286I G_dGCh'C

Cuts at:    549     769     966    1246    1857    1950    2024    2214    2276 
   Size:        220     197     280     611      93      74     190      62

Cuts at:   2276    3006     549 
   Size:        730    1775

  Fragments arranged by size: 

                1775     730     611     280     220     197     190      93
                  74      62
 
BspEI T'CCGG_A

Cuts at:   3452    3452 
   Size:       4232
 
BspHI T'CATG_A

Cuts at:   3408    3408 
   Size:       4232
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    275     746    1282    1496    1798    2179     275 
   Size:        471     536     214     302     381    2328

  Fragments arranged by size: 

                2328     536     471     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50     359    2524    2578    4062    4227      50 
   Size:        309    2165      54    1484     165      55

  Fragments arranged by size: 

                2165    1484     309     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    995    2144     995 
   Size:       1149    3083
 
BsrFI r'CCGG_y

Cuts at:    126     163     270     551     590    2230    2411    2620     126 
   Size:         37     107     281      39    1640     181     209    1738

  Fragments arranged by size: 

                1738    1640     281     209     181     107      39      37
            
 
BsrGI T'GTAC_A

Cuts at:    371     371 
   Size:       4232
 
BssSI C'ACGA_G

Cuts at:   2503    2861    2503 
   Size:        358    3874
 
BstAPI GCAn_nnn'nTGC

Cuts at:    994    1609     994 
   Size:        615    3617
 
BstBI TT'CG_AA

Cuts at:   2593    2593 
   Size:       4232
 
BstYI r'GATC_y

Cuts at:      1     458     849    1257    1724    1747    2082    2328    3340 
   Size:        457     391     408     467      23     335     246    1012

Cuts at:   3340    3426    3438    3784       1 
   Size:         86      12     346     449

  Fragments arranged by size: 

                1012     467     457     449     408     391     346     335
                 246      86      23      12
 
BtgI C'CryG_G

Cuts at:    773     773 
   Size:       4232
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2182    2363    2526    2182 
   Size:        181     163    3888

  Fragments arranged by size: 

                3888     181     163
 
BtsI GCAGTG_nn'

Cuts at:    203    1475     203 
   Size:       1272    2960
 
ClaI AT'CG_AT

Cuts at:    880     880 
   Size:       4232
 
DraI TTT'AAA

Cuts at:   1231    1231 
   Size:       4232
 
DrdI GACnn_nn'nnGTC

Cuts at:   1938    2796    1938 
   Size:        858    3374
 
EaeI y'GGCC_r

Cuts at:    124     161     320     392     776    1817    1991    2382    2409 
   Size:         37     159      72     384    1041     174     391      27

Cuts at:   2409    3754     124 
   Size:       1345     602

  Fragments arranged by size: 

                1345    1041     602     391     384     174     159      72
                  37      27
 
EagI C'GGCC_G

Cuts at:   1817    1817 
   Size:       4232
 
EarI CTCTTCn'nnn_

Cuts at:    673    2255    2465     673 
   Size:       1582     210    2440

  Fragments arranged by size: 

                2440    1582     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2440    2750    2896    4042    2440 
   Size:        310     146    1146    2630

  Fragments arranged by size: 

                2630    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   1244    1244 
   Size:       4232
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    375    2057    2489    3236    3504     375 
   Size:       1682     432     747     268    1103

  Fragments arranged by size: 

                1682    1103     747     432     268
 
EcoO109I rG'GnC_Cy

Cuts at:    962    1371     962 
   Size:        409    3823
 
EcoRI G'AATT_C

Cuts at:   1236    1236 
   Size:       4232
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    267    1148    2251     267 
   Size:        881    1103    2248

  Fragments arranged by size: 

                2248    1103     881
 
FspI TGC'GCA

Cuts at:   2013    2013 
   Size:       4232
 
HaeII r_GCGC'y

Cuts at:   1666    1914    2936    3915    1666 
   Size:        248    1022     979    1983

  Fragments arranged by size: 

                1983    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    709     741    1023    1055    2074    2104    2106    2136     709 
   Size:         32     282      32    1019      30       2      30    2805

  Fragments arranged by size: 

                2805    1019     282      32      32      30      30       2
            
 
HincII GTy'rAC

Cuts at:   1271    1271 
   Size:       4232
 
HindIII A'AGCT_T

Cuts at:   1130    1130 
   Size:       4232
 
KasI G'GCGC_C

Cuts at:   1662    1910    1662 
   Size:        248    3984
 
KpnI G_GTAC'C

Cuts at:    683    1252     683 
   Size:        569    3663
 
MluI A'CGCG_T

Cuts at:   3665    3665 
   Size:       4232
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2903    3087    2903 
   Size:        184    4048
 
MscI TGG'CCA

Cuts at:    322     778    1993     322 
   Size:        456    1215    2561

  Fragments arranged by size: 

                2561    1215     456
 
MslI CAynn'nnrTG

Cuts at:    328     367     643     772    2221    2348     328 
   Size:         39     276     129    1449     127    2212

  Fragments arranged by size: 

                2212    1449     276     129     127      39
 
MspA1I CmG'CkG

Cuts at:    560    1343    1657    2017    3030    3275     560 
   Size:        783     314     360    1013     245    1517

  Fragments arranged by size: 

                1517    1013     783     360     314     245
 
NaeI GCC'GGC

Cuts at:    128     272     592    2413     128 
   Size:        144     320    1821    1947

  Fragments arranged by size: 

                1947    1821     320     144
 
NarI GG'CG_CC

Cuts at:   1663    1911    1663 
   Size:        248    3984
 
NgoMIV G'CCGG_C

Cuts at:    126     270     590    2411     126 
   Size:        144     320    1821    1947

  Fragments arranged by size: 

                1947    1821     320     144
 
NsiI A_TGCA'T

Cuts at:    541     541 
   Size:       4232
 
NspI r_CATG'y

Cuts at:    378     539     765    1285    1785    2692     378 
   Size:        161     226     520     500     907    1918

  Fragments arranged by size: 

                1918     907     520     500     226     161
 
PasI CC'CwG_GG

Cuts at:    585     585 
   Size:       4232
 
PciI A'CATG_T

Cuts at:    374    2688     374 
   Size:       2314    1918
 
PmeI GTTT'AAAC

Cuts at:   1231    1231 
   Size:       4232
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3371    3403    3371 
   Size:         32    4200
 
PspOMI G'GGCC_C

Cuts at:    962     962 
   Size:       4232
 
PstI C_TGCA'G

Cuts at:    488    1279    1964     488 
   Size:        791     685    2756

  Fragments arranged by size: 

                2756     791     685
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4232
 
PvuII CAG'CTG

Cuts at:   1657    2017    1657 
   Size:        360    3872
 
RsrII CG'GwC_CG

Cuts at:    166    2427     166 
   Size:       2261    1971
 
SacI G_AGCT'C

Cuts at:   1246    1246 
   Size:       4232
 
SalI G'TCGA_C

Cuts at:   1269    1269 
   Size:       4232
 
SapI GCTCTTCn'nnn_

Cuts at:   2255    2465    2255 
   Size:        210    4022
 
SbfI CC_TGCA'GG

Cuts at:   1279    1279 
   Size:       4232
 
ScaI AGT'ACT

Cuts at:   3749    3749 
   Size:       4232
 
SexAI A'CCwGG_T

Cuts at:    887     887 
   Size:       4232
 
SfcI C'TryA_G

Cuts at:     33     484     756    1275    1960    2953    3144      33 
   Size:        451     272     519     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     519     451     272     191
 
SfoI GGC'GCC

Cuts at:   1664    1912    1664 
   Size:        248    3984
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4232
 
SmaI CCC'GGG

Cuts at:   1254    1254 
   Size:       4232
 
SmlI C'TyrA_G

Cuts at:    577     957    1387    2302    2794    3056     577 
   Size:        380     430     915     492     262    1753

  Fragments arranged by size: 

                1753     915     492     430     380     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4232
 
SphI G_CATG'C

Cuts at:   1285    1785    1285 
   Size:        500    3732
 
SspI AAT'ATT

Cuts at:   1188    1188 
   Size:       4232
 
StyI C'CwwG_G

Cuts at:    794    1366    1720     794 
   Size:        572     354    3306

  Fragments arranged by size: 

                3306     572     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    127    1815    1948    2407    2616     127 
   Size:       1688     133     459     209    1743

  Fragments arranged by size: 

                1743    1688     459     209     133
 
TatI w'GTAC_w

Cuts at:    371     900    3747     371 
   Size:        529    2847     856

  Fragments arranged by size: 

                2847     856     529
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    460     492     812     844    3749    3781     460 
   Size:         32     320      32    2905      32     911

  Fragments arranged by size: 

                2905     911     320      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   2029    2029 
   Size:       4232
 
XbaI T'CTAG_A

Cuts at:   1135    1263    1135 
   Size:        128    4104
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1727    1727 
   Size:       4232
 
XmaI C'CCGG_G

Cuts at:   1252    1252 
   Size:       4232
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AhdI     AleI
    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI     BaeI
    BamHI     BanI    BanII     BbeI     BbsI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI Bme1580I     BmrI     BplI     BpmI    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseRI    BseYI    BsiEI
  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI    BssSI   BstAPI    BstBI    BstYI     BtgI
    BtgZI     BtsI     ClaI     DraI     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII
    Hin4I   HincII  HindIII     KasI     KpnI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI   NgoMIV     NsiI     NspI     PasI
     PciI     PmeI     PpiI   PspOMI     PstI     PvuI    PvuII    RsrII
     SacI     SalI     SapI     SbfI     ScaI    SexAI     SfcI     SfoI
     SgfI     SmaI     SmlI     SpeI     SphI     SspI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AlfI     AloI     AscI    AvrII
    BbvCI     BcgI    BmgBI     BmtI   Bpu10I    BsaXI     BsgI    BsiWI
   BssHII   BstEII    BstXI  BstZ17I   Bsu36I    CspCI   DraIII    EcoNI
    EcoRV     FseI    FspAI     HpaI     MfeI     NcoI     NdeI     NheI
     NotI     NruI     PacI    PflMI     PfoI     PmlI    PpuMI    PshAI
     PsiI    PspXI     PsrI    SacII    SanDI     SfiI    SgrAI    SnaBI
     SrfI     StuI     SwaI     XhoI     XmnI     ZraI
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