Restriction map for pF1KB9845
 (Linear) (Six-base) MAPSORT of: KIBB9845.seq  Check: 1114  from: 1  to: 1119



KIBB9845.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:12  ..

AccI GT'mk_AC

Cuts at:      0     170    1119 
   Size:        170     949
 
Acc65I G'GTAC_C

Cuts at:      0     571    1119 
   Size:        571     548
 
AflIII A'CryG_T

Cuts at:      0     266    1119 
   Size:        266     853
 
AgeI A'CCGG_T

Cuts at:      0     443    1119 
   Size:        443     676
 
AhdI GACnn_n'nnGTC

Cuts at:      0     929    1119 
   Size:        929     190
 
AleI CACnn'nnGTG

Cuts at:      0     664    1119 
   Size:        664     455
 
ApaI G_GGCC'C

Cuts at:      0     858    1119 
   Size:        858     261
 
ApaLI G'TGCA_C

Cuts at:      0     657    1119 
   Size:        657     462
 
ApoI r'AATT_y

Cuts at:      0    1044    1119 
   Size:       1044      75
 
AseI AT'TA_AT

Cuts at:      0     715    1119 
   Size:        715     404
 
AvaI C'yCGr_G

Cuts at:      0    1007    1119 
   Size:       1007     112
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     438     471     555     563     588     596    1119 
   Size:        438      33      84       8      25       8     523

  Fragments arranged by size: 

                 523     438      84      33      25       8       8
 
BanI G'GyrC_C

Cuts at:      0     571    1119 
   Size:        571     548
 
BanII G_rGCy'C

Cuts at:      0     858    1119 
   Size:        858     261
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     222    1070    1119 
   Size:        222     848      49

  Fragments arranged by size: 

                 848     222      49
 
BfrBI ATG'CAT

Cuts at:      0     431    1119 
   Size:        431     688
 
BglI GCCn_nnn'nGGC

Cuts at:      0      52    1119 
   Size:         52    1067
 
BglII A'GATC_T

Cuts at:      0     741    1119 
   Size:        741     378
 
Bme1580I G_kGCm'C

Cuts at:      0     441     661     858    1119 
   Size:        441     220     197     261

  Fragments arranged by size: 

                 441     261     220     197
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     267    1119 
   Size:        267     852
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     454     870    1119 
   Size:        454     416     249

  Fragments arranged by size: 

                 454     416     249
 
BsaAI yAC'GTr

Cuts at:      0     300     914    1119 
   Size:        300     614     205

  Fragments arranged by size: 

                 614     300     205
 
BsaBI GATnn'nnATC

Cuts at:      0     508    1119 
   Size:        508     611
 
BsaWI w'CCGG_w

Cuts at:      0     443    1119 
   Size:        443     676
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      99     746     777    1119 
   Size:         99     647      31     342

  Fragments arranged by size: 

                 647     342      99      31
 
BseYI C'CCAG_C

Cuts at:      0     876    1119 
   Size:        876     243
 
BsiEI CG_ry'CG

Cuts at:      0      89     665    1119 
   Size:         89     576     454

  Fragments arranged by size: 

                 576     454      89
 
BsiHKAI G_wGCw'C

Cuts at:      0     661    1119 
   Size:        661     458
 
BsmI GAATG_Cn'

Cuts at:      0     504    1119 
   Size:        504     615
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     111    1119 
   Size:        111    1008
 
Bsp1286I G_dGCh'C

Cuts at:      0     441     661     858    1119 
   Size:        441     220     197     261

  Fragments arranged by size: 

                 441     261     220     197
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     167     638    1119 
   Size:        167     471     481

  Fragments arranged by size: 

                 481     471     167
 
BsrBI CCG'CTC

Cuts at:      0     251    1119 
   Size:        251     868
 
BsrDI GCAATG_nn'

Cuts at:      0     887    1119 
   Size:        887     232
 
BsrFI r'CCGG_y

Cuts at:      0      18      55     162     443     482    1119 
   Size:         18      37     107     281      39     637

  Fragments arranged by size: 

                 637     281     107      39      37      18
 
BsrGI T'GTAC_A

Cuts at:      0     263    1119 
   Size:        263     856
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     886    1119 
   Size:        886     233
 
BstYI r'GATC_y

Cuts at:      0     350     741    1119 
   Size:        350     391     378

  Fragments arranged by size: 

                 391     378     350
 
BtgI C'CryG_G

Cuts at:      0     665    1119 
   Size:        665     454
 
BtsI GCAGTG_nn'

Cuts at:      0      95    1119 
   Size:         95    1024
 
ClaI AT'CG_AT

Cuts at:      0     772    1119 
   Size:        772     347
 
EaeI y'GGCC_r

Cuts at:      0      16      53     212     284     668    1119 
   Size:         16      37     159      72     384     451

  Fragments arranged by size: 

                 451     384     159      72      37      16
 
EarI CTCTTCn'nnn_

Cuts at:      0     565    1119 
   Size:        565     554
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     267    1119 
   Size:        267     852
 
EcoO109I rG'GnC_Cy

Cuts at:      0     854    1119 
   Size:        854     265
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     159    1040    1119 
   Size:        159     881      79

  Fragments arranged by size: 

                 881     159      79
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     601     633     915     947    1119 
   Size:        601      32     282      32     172

  Fragments arranged by size: 

                 601     282     172      32      32
 
HindIII A'AGCT_T

Cuts at:      0    1022    1119 
   Size:       1022      97
 
KpnI G_GTAC'C

Cuts at:      0     575    1119 
   Size:        575     544
 
MscI TGG'CCA

Cuts at:      0     214     670    1119 
   Size:        214     456     449

  Fragments arranged by size: 

                 456     449     214
 
MslI CAynn'nnrTG

Cuts at:      0     220     259     535     664    1119 
   Size:        220      39     276     129     455

  Fragments arranged by size: 

                 455     276     220     129      39
 
MspA1I CmG'CkG

Cuts at:      0     452    1119 
   Size:        452     667
 
NaeI GCC'GGC

Cuts at:      0      20     164     484    1119 
   Size:         20     144     320     635

  Fragments arranged by size: 

                 635     320     144      20
 
NgoMIV G'CCGG_C

Cuts at:      0      18     162     482    1119 
   Size:         18     144     320     637

  Fragments arranged by size: 

                 637     320     144      18
 
NsiI A_TGCA'T

Cuts at:      0     433    1119 
   Size:        433     686
 
NspI r_CATG'y

Cuts at:      0     270     431     657    1119 
   Size:        270     161     226     462

  Fragments arranged by size: 

                 462     270     226     161
 
PasI CC'CwG_GG

Cuts at:      0     477    1119 
   Size:        477     642
 
PciI A'CATG_T

Cuts at:      0     266    1119 
   Size:        266     853
 
PspOMI G'GGCC_C

Cuts at:      0     854    1119 
   Size:        854     265
 
PstI C_TGCA'G

Cuts at:      0     380    1119 
   Size:        380     739
 
RsrII CG'GwC_CG

Cuts at:      0      58    1119 
   Size:         58    1061
 
SexAI A'CCwGG_T

Cuts at:      0     779    1119 
   Size:        779     340
 
SfcI C'TryA_G

Cuts at:      0     376     648    1119 
   Size:        376     272     471

  Fragments arranged by size: 

                 471     376     272
 
SmlI C'TyrA_G

Cuts at:      0     469     849    1119 
   Size:        469     380     270

  Fragments arranged by size: 

                 469     380     270
 
SspI AAT'ATT

Cuts at:      0    1080    1119 
   Size:       1080      39
 
StyI C'CwwG_G

Cuts at:      0     686    1119 
   Size:        686     433
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      19    1119 
   Size:         19    1100
 
TatI w'GTAC_w

Cuts at:      0     263     792    1119 
   Size:        263     529     327

  Fragments arranged by size: 

                 529     327     263
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     352     384     704     736    1119 
   Size:        352      32     320      32     383

  Fragments arranged by size: 

                 383     352     320      32      32
 
XbaI T'CTAG_A

Cuts at:      0    1027    1119 
   Size:       1027      92
 

 Enzymes that do cut:

     AccI   Acc65I   AflIII     AgeI     AhdI     AleI     ApaI    ApaLI
     ApoI     AseI     AvaI     BaeI     BanI    BanII     BbsI    BfrBI
     BglI    BglII Bme1580I     BpmI    BpuEI    BsaAI    BsaBI    BsaWI
    BseRI    BseYI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI   BstAPI    BstYI     BtgI     BtsI
     ClaI     EaeI     EarI  Eco57MI EcoO109I     FalI    Hin4I  HindIII
     KpnI     MscI     MslI   MspA1I     NaeI   NgoMIV     NsiI     NspI
     PasI     PciI   PspOMI     PstI    RsrII    SexAI     SfcI     SmlI
     SspI     StyI    TaqII     TatI     TstI     XbaI

 Enzymes that do not cut: 

     AarI    AatII     AclI     AcuI     AfeI    AflII     AlfI     AloI
    AlwNI     AscI    AsiSI    AvrII    BamHI     BbeI    BbvCI     BcgI
    BciVI     BclI     BlpI    BmgBI     BmrI     BmtI     BplI   Bpu10I
     BsaI    BsaHI    BsaXI     BsgI    BsiWI    BspEI    BspHI   BssHII
    BssSI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI    CspCI
     DraI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI    EcoRI
    EcoRV     FseI     FspI    FspAI    HaeII   HincII     HpaI     KasI
     MfeI     MluI     MmeI     NarI     NcoI     NdeI     NheI     NotI
     NruI     PacI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI    PspXI     PsrI     PvuI    PvuII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     StuI     SwaI
  Tth111I     XcmI     XhoI     XmaI     XmnI     ZraI
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