Restriction map for pF1KB9843
##################################
# NO CUT ENZYMES (  157 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      AocI        Aor51HI     
ApaI        ApaLI       AscI        Asp718I     AspEI       
AspI        AvaI        AvaII       AviII       AvrII       
BamHI       BanII       BbeI        BbvCI       BcgI        
BciVI       BfrI        BglI        BlnI        BlpI        
Bpu1102I    BsaHI       BsaI        BsaWI       BseAI       
BsiEI       BsiWI       BsmBI       BsoBI       Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstZ17I     Bsu36I      CelII       CfoI        
Cfr10I      Cfr13I      ClaI        CpoI        DraI        
DraII       DraIII      DrdI        DsaI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRV       
FauI        FseI        FspI        HaeII       HgaI        
HhaI        Hin1I       HinP1I      HpaI        KasI        
KpnI        Ksp632I     KspI        MfeI        MluI        
MroI        MslI        MspA1I      MunI        MvnI        
NaeI        NarI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmeI        
PpuMI       PshAI       Psp1406I    PstI        PvuI        
PvuII       RcaI        RsrII       SacI        SacII       
SalI        SapI        Sau96I      SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        Sse8387I    
SspBI       SspI        StuI        SwaI        TfiI        
Tth111I     Van91I      XbaI        XcmI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   55 enzymes) #
###################################

AlwNI         377  CAGATGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AseI          888  ATTAAT               AT^TAAT
AsnI          888  ATTAAT               AT^TAAT
Asp700I      1065  GAAAGCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         324  GTGCTC               G[AT]GC[AT]^C
BalI          678  TGGCCA               TGG^CCA
BanI          485  GGCACC               G^G[CT][GA]CC
BbrPI         321  CACGTG               CAC^GTG
BbsI         1054  GAAGAC               GAAGAC(2/6)
BclI           21  TGATCA               T^GATCA
BcnI          152  CCCGG                CC^[GC]GG
BglII         845  AGATCT               A^GATCT
BmyI          324  GTGCTC               G[AGT]GC[ACT]^C
BpmI          480  CTGGAG               CTGGAG(16/14)
BpuAI        1054  GAAGAC               GAAGAC(2/6)
BsaAI         321  CACGTG               [CT]AC^GT[GA]
BsiHKAI       324  GTGCTC               G[AT]GC[AT]^C
BsiYI         395  CCAGGAAAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          395  CCAGGAAAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI          78  GTCCC                GGGAC(10/14)
Bsp1286I      324  GTGCTC               G[AGT]GC[ACT]^C
BspMI         313  ACCTGC               ACCTGC(4/8)
BsrI          579  ACTGG                ACTGG(1/-1)
BstYI         845  AGATCT               [GA]^GATC[CT]
EaeI          678  TGGCCA               [CT]^GGCC[GA]
EcoRI         811  GAATTC               G^AATTC
EcoT14I       489  CCAAGG               C^C[AT][AT]GG
EcoT22I       513  ATGCAT               ATGCA^T
FbaI           21  TGATCA               T^GATCA
HaeIII        679  GGCC                 GG^CC
HapII         153  CCGG                 C^CGG
HincII        304  GTTGAC               GT[CT]^[GA]AC
HindII        304  GTTGAC               GT[CT]^[GA]AC
HindIII      1078  AAGCTT               A^AGCTT
HinfI         405  GACTC                G^A[ACGT]TC
HpaII         153  CCGG                 C^CGG
MaeII         322  ACGT                 A^CGT
MflI          845  AGATCT               [GA]^GATC[CT]
MluNI         678  TGGCCA               TGG^CCA
MscI          678  TGGCCA               TGG^CCA
MspI          153  CCGG                 C^CGG
MwoI          317  GCTGCACGTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI          152  CCCGG                CC^[GC]GG
NlaIV         485  GGCACC               GG[ACGT]^[ACGT]CC
NsiI          513  ATGCAT               ATGCA^T
NspI          414  GCATGC               [GA]CATG^[CT]
PleI          405  GACTC                GAGTC(4/5)
PmaCI         321  CACGTG               CAC^GTG
PmlI          321  CACGTG               CAC^GTG
PshBI         888  ATTAAT               AT^TAAT
SfcI          347  CTATAG               C^T[GA][CT]AG
SphI          414  GCATGC               GCATG^C
StyI          489  CCAAGG               C^C[AT][AT]GG
XhoII         845  AGATCT               [GA]^GATC[CT]
XmnI         1065  GAAAGCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   13 enzymes) #
###################################

AfaI          736  GTAC                 GT^AC
              854  GTAC                
AlwI          594  GATCC                GGATC(4/5)
              357  GGATC               
BfaI          497  CTAG                 C^TAG
              739  CTAG                
BsaBI         358  GATCGGTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              588  GATTTTGATC          
BseRI         470  CTCCTC               GAGGAG(10/8)
              667  GAGGAG              
BsgI          160  CTGCAC               GTGCAG(16/14)
              318  CTGCAC              
BsmAI         642  GAGAC                GTCTC(1/5)
              800  GTCTC               
MaeI          497  CTAG                 C^TAG
              739  CTAG                
MamI          358  GATCGGTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              588  GATTTTGATC          
MboII         976  GAAGA                GAAGA(8/7)
             1054  GAAGA               
RsaI          736  GTAC                 GT^AC
              854  GTAC                
TaqI          366  TCGA                 T^CGA
             1092  TCGA                
TthHB8I       366  TCGA                 T^CGA
             1092  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   33 enzymes) #
#########################################

AcsI        AluI        ApoI        BbvI        BsaJI       
BsmI        BssKI       BstF5I      BstNI       BstXI       
Cac8I       DdeI        DpnII       EcoRII      Fnu4HI      
FokI        HphI        ItaI        MaeIII      MboI        
MnlI        MseI        MvaI        NdeII       NlaIII      
Sau3AI      ScrFI       SfaNI       Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       
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