Restriction map for pF1KB9842
##################################
# NO CUT ENZYMES (  138 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AocI        ApaI        ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AvaI        AviII       AvrII       
BanI        BbeI        BbrPI       BbsI        BbvCI       
BciVI       BfrI        BglI        BlnI        BpmI        
BpuAI       BsaAI       BsaBI       BsaHI       BsaWI       
BseAI       BsgI        BsiHKAI     BsmBI       BsmFI       
BsmI        BsoBI       Bsp1407I    BspDI       BspEI       
BspLU11I    BsrDI       BsrFI       BsrGI       BssHII      
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstZ17I     Bsu36I      Cfr10I      ClaI        
CpoI        DraI        DraIII      DrdI        Eam1105I    
Eco81I      EcoO65I     EcoRI       EcoRV       EcoT22I     
FseI        FspI        HgaI        Hin1I       HindIII     
HpaI        KasI        KpnI        KspI        MaeII       
MamI        MfeI        MluI        MroI        MunI        
MvnI        NaeI        NarI        NgoMIV      NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PstI        PvuI        RsrII       SacI        
SacII       SalI        SbfI        ScaI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
SwaI        Tth111I     Van91I      XbaI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AcsI          487  AAATTC               [GA]^AATT[CT]
AfaI          707  GTAC                 GT^AC
AlwNI         501  CAGCATCTG            CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI       473  AGCGCT               AGC^GCT
ApoI          487  AAATTC               [GA]^AATT[CT]
BamHI         635  GGATCC               G^GATCC
BanII         881  GGGCTC               G[GA]GC[CT]^C
BcgI          866  GCAATCACATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII          54  AGATCT               A^GATCT
BlpI          180  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      180  GCTGAGC              GC^T[ACGT]AGC
BseRI         973  CTCCTC               GAGGAG(10/8)
BsiWI         706  CGTACG               C^GTACG
BspHI         827  TCATGA               T^CATGA
BspMI         257  ACCTGC               ACCTGC(4/8)
BsrBI         237  CCGCTC               CCGCTC(-3/-3)
BssSI         202  CTCGTG               CACGAG(-5/-1)
BstXI         645  CCAGTCCTTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII         180  GCTGAGC              GC^T[ACGT]AGC
DraII         832  AGGACCC              [GA]G^G[ACGT]CC[CT]
DsaI          966  CCATGG               C^C[GA][CT]GG
EagI          234  CGGCCG               C^GGCCG
EarI         1050  CTCTTC               CTCTTC(1/4)
EclXI         234  CGGCCG               C^GGCCG
Eco47III      473  AGCGCT               AGC^GCT
Eco52I        234  CGGCCG               C^GGCCG
EcoNI         351  CCTGTGCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      832  AGGACCC              [GA]G^G[ACGT]CC[CT]
FauI          750  GCGGG                CCCGC(4/6)
HincII        382  GTCAAC               GT[CT]^[GA]AC
HindII        382  GTCAAC               GT[CT]^[GA]AC
Ksp632I      1050  CTCTTC               CTCTTC(1/4)
MspA1I        918  CAGCTG               C[AC]G^C[GT]G
NcoI          966  CCATGG               C^CATGG
NdeI          492  CATATG               CA^TATG
PpuMI         832  AGGACCC              [GA]G^G[AT]CC[CT]
PvuII         918  CAGCTG               CAG^CTG
RcaI          827  TCATGA               T^CATGA
RsaI          707  GTAC                 GT^AC
SapI         1049  GCTCTTC              GCTCTTC(1/4)
SexAI         803  ACCTGGT              A^CC[AT]GGT
SfcI          390  CTATAG               C^T[GA][CT]AG
SmlI          976  CTCAAG               C^T[CT][GA]AG
StuI         1033  AGGCCT               AGG^CCT
TaqI          875  TCGA                 T^CGA
TthHB8I       875  TCGA                 T^CGA
XcmI          893  CCACCGAGATTCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AccI          253  GTCTAC               GT^[AC][GT]AC
              559  GTCTAC              
BalI          437  TGGCCA               TGG^CCA
              951  TGGCCA              
BbvI          792  GCTGC                GCAGC(8/12)
              920  GCTGC               
BclI          674  TGATCA               T^GATCA
             1001  TGATCA              
BcnI          741  CCGGG                CC^[GC]GG
              879  CCGGG               
BmyI          881  GGGCTC               G[AGT]GC[ACT]^C
             1060  GGGCAC              
BsaI          850  GAGACC               GGTCTC(1/5)
              363  GGTCTC              
BsiEI         234  CGGCCG               CG[GA][CT]^CG
              876  CGACCG              
Bsp1286I      881  GGGCTC               G[AGT]GC[ACT]^C
             1060  GGGCAC              
BstYI          54  AGATCT               [GA]^GATC[CT]
              635  GGATCC              
CfoI          474  GCGC                 GCG^C
              861  GCGC                
FbaI          674  TGATCA               T^GATCA
             1001  TGATCA              
HaeII         473  AGCGCT               [GA]GCGC^[CT]
              860  AGCGCC              
HapII         741  CCGG                 C^CGG
              879  CCGG                
HhaI          474  GCGC                 GCG^C
              861  GCGC                
HinP1I        474  GCGC                 G^CGC
              861  GCGC                
HpaII         741  CCGG                 C^CGG
              879  CCGG                
MflI           54  AGATCT               [GA]^GATC[CT]
              635  GGATCC              
MluNI         437  TGGCCA               TGG^CCA
              951  TGGCCA              
MscI          437  TGGCCA               TGG^CCA
              951  TGGCCA              
MseI           11  TTAA                 T^TAA
              716  TTAA                
MspI          741  CCGG                 C^CGG
              879  CCGG                
NciI          741  CCGGG                CC^[GC]GG
              879  CCGGG               
NlaIV         635  GGATCC               GG[ACGT]^[ACGT]CC
              833  GGACCC              
PleI          971  GACTC                GAGTC(4/5)
             1012  GACTC               
Tru9I          11  TTAA                 T^TAA
              716  TTAA                
TseI          792  GCTGC                G^C[AT]GC
              920  GCTGC               
Tsp45I         23  GTGAC                ^GT[GC]AC
              244  GTCAC               
Tsp509I        64  AATT                 ^AATT
              488  AATT                
XhoII          54  AGATCT               [GA]^GATC[CT]
              635  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   43 enzymes) #
#########################################

AciI        AluI        AlwI        AvaII       BfaI        
BsaJI       BsiYI       BslI        BsmAI       BsrI        
BssKI       BstF5I      BstNI       Cac8I       Cfr13I      
DdeI        DpnII       EaeI        EcoRII      EcoT14I     
Fnu4HI      FokI        HaeIII      HinfI       HphI        
ItaI        MaeI        MaeIII      MboI        MboII       
MnlI        MslI        MvaI        MwoI        NdeII       
NlaIII      Sau3AI      Sau96I      ScrFI       SfaNI       
StyI        TfiI        TspRI       
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