Restriction map for pF1KB9837
 (Circular) (Six-base) MAPSORT of: pF1KB9837.seq  Check: 8932  from: 1  to: 4169



pF1KB9837  4169 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:32  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    646     936     646 
   Size:        290    3879
 
AccI GT'mk_AC

Cuts at:   1207    1207 
   Size:       4169
 
Acc65I G'GTAC_C

Cuts at:    485    1185     485 
   Size:        700    3469
 
AclI AA'CG_TT

Cuts at:   4009    4009 
   Size:       4169
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    310    1994    2426    3173    3441     310 
   Size:       1684     432     747     268    1038

  Fragments arranged by size: 

                1684    1038     747     432     268
 
AflIII A'CryG_T

Cuts at:   1521    2625    3602    1521 
   Size:       1104     977    2088

  Fragments arranged by size: 

                2088    1104     977
 
AgeI A'CCGG_T

Cuts at:   2557    2557 
   Size:       4169
 
AleI CACnn'nnGTG

Cuts at:    565     565 
   Size:       4169
 
AlwNI CAG_nnn'CTG

Cuts at:   1474    3041    1474 
   Size:       1567    2602
 
ApaLI G'TGCA_C

Cuts at:   2939    2939 
   Size:       4169
 
ApoI r'AATT_y

Cuts at:    681     892    1173    3522    3778     681 
   Size:        211     281    2349     256    1072

  Fragments arranged by size: 

                2349    1072     281     256     211
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4169
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4169
 
AvaI C'yCGr_G

Cuts at:    750    1102    1189     750 
   Size:        352      87    3730

  Fragments arranged by size: 

                3730     352      87
 
BamHI G'GATC_C

Cuts at:   1194    1194 
   Size:       4169
 
BanI G'GyrC_C

Cuts at:    485    1185    1599    1847    1882     485 
   Size:        700     414     248      35    2772

  Fragments arranged by size: 

                2772     700     414     248      35
 
BanII G_rGCy'C

Cuts at:    164     369     428     855    1183    2213     164 
   Size:        205      59     427     328    1030    2120

  Fragments arranged by size: 

                2120    1030     427     328     205      59
 
BbeI G_GCGC'C

Cuts at:   1603    1851    1603 
   Size:        248    3921
 
BbsI GAAGACnn'nnnn_

Cuts at:    619    1029     619 
   Size:        410    3759
 
BciVI GTATCCnnnnn_n'

Cuts at:   2067    2828    4169    2067 
   Size:        761    1341    2067

  Fragments arranged by size: 

                2067    1341     761
 
BclI T'GATC_A

Cuts at:    557    1689     557 
   Size:       1132    3037
 
BglII A'GATC_T

Cuts at:    610    1684    3721     610 
   Size:       1074    2037    1058

  Fragments arranged by size: 

                2037    1074    1058
 
BlpI GC'TnA_GC

Cuts at:   1281    1281 
   Size:       4169
 
Bme1580I G_kGCm'C

Cuts at:   1794    1887    2943    1794 
   Size:         93    1056    3020

  Fragments arranged by size: 

                3020    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    378    1145    1381    1462    1797    3941     378 
   Size:        767     236      81     335    2144     606

  Fragments arranged by size: 

                2144     767     606     335     236      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2536    2536 
   Size:       4169
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1345    2224    2716    3014    1345 
   Size:        879     492     298    2500

  Fragments arranged by size: 

                2500     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3873    3873 
   Size:       4169
 
BsaAI yAC'GTr

Cuts at:   1522    1522 
   Size:       4169
 
BsaBI GATnn'nnATC

Cuts at:      6     703    1683    3720       6 
   Size:        697     980    2037     455

  Fragments arranged by size: 

                2037     980     697     455
 
BsaHI Gr'CG_yC

Cuts at:   1600    1848    2550    4046    1600 
   Size:        248     702    1496    1723

  Fragments arranged by size: 

                1723    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:   1358    1879    2557    2831    2978    3389    1358 
   Size:        521     678     274     147     411    2138

  Fragments arranged by size: 

                2138     678     521     411     274     147
 
BseYI C'CCAG_C

Cuts at:   1594    2352    2929    3425    1594 
   Size:        758     577     496    2338

  Fragments arranged by size: 

                2338     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104    1711    1757    2965     104 
   Size:       1607      46    1208    1308

  Fragments arranged by size: 

                1607    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:   1183    1961    2151    2943    1183 
   Size:        778     190     792    2409

  Fragments arranged by size: 

                2409     792     778     190
 
BsmBI CGTCTCn'nnnn_

Cuts at:    590     590 
   Size:       4169
 
Bsp1286I G_dGCh'C

Cuts at:    164     369     428     855    1183    1794    1887    1961    2151 
   Size:        205      59     427     328     611      93      74     190

Cuts at:   2151    2213    2943     164 
   Size:         62     730    1390

  Fragments arranged by size: 

                1390     730     611     427     328     205     190      93
                  74      62      59
 
BspEI T'CCGG_A

Cuts at:   3389    3389 
   Size:       4169
 
BspHI T'CATG_A

Cuts at:    827    3345     827 
   Size:       2518    1651
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    320     646     936    1219    1433    1735    2116     320 
   Size:        326     290     283     214     302     381    2373

  Fragments arranged by size: 

                2373     381     326     302     290     283     214
 
BsrBI CCG'CTC

Cuts at:     50     213    2461    2515    3999    4164      50 
   Size:        163    2248      54    1484     165      55

  Fragments arranged by size: 

                2248    1484     165     163      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1052    2081    1052 
   Size:       1029    3140
 
BsrFI r'CCGG_y

Cuts at:   2167    2348    2557    2167 
   Size:        181     209    3779

  Fragments arranged by size: 

                3779     209     181
 
BssSI C'ACGA_G

Cuts at:   2440    2798    2440 
   Size:        358    3811
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1546    1546 
   Size:       4169
 
BstBI TT'CG_AA

Cuts at:   2530    2530 
   Size:       4169
 
BstEII G'GTnAC_C

Cuts at:    531     531 
   Size:       4169
 
BstYI r'GATC_y

Cuts at:      1     610    1194    1661    1684    2019    2265    3277    3363 
   Size:        609     584     467      23     335     246    1012      86

Cuts at:   3363    3375    3721       1 
   Size:         12     346     449

  Fragments arranged by size: 

                1012     609     584     467     449     346     335     246
                  86      23      12
 
BtgI C'CryG_G

Cuts at:    107     107 
   Size:       4169
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2119    2300    2463    2119 
   Size:        181     163    3825

  Fragments arranged by size: 

                3825     181     163
 
BtsI GCAGTG_nn'

Cuts at:    972    1412     972 
   Size:        440    3729
 
ClaI AT'CG_AT

Cuts at:    479     479 
   Size:       4169
 
DraI TTT'AAA

Cuts at:    518    1168     518 
   Size:        650    3519
 
DrdI GACnn_nn'nnGTC

Cuts at:   1875    2733    1875 
   Size:        858    3311
 
EaeI y'GGCC_r

Cuts at:    323    1754    1928    2319    2346    3691     323 
   Size:       1431     174     391      27    1345     801

  Fragments arranged by size: 

                1431    1345     801     391     174      27
 
EagI C'GGCC_G

Cuts at:   1754    1754 
   Size:       4169
 
EarI CTCTTCn'nnn_

Cuts at:    286     787     850    2192    2402     286 
   Size:        501      63    1342     210    2053

  Fragments arranged by size: 

                2053    1342     501     210      63
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2377    2687    2833    3979    2377 
   Size:        310     146    1146    2567

  Fragments arranged by size: 

                2567    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   1181    1181 
   Size:       4169
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    310    1994    2426    3173    3441     310 
   Size:       1684     432     747     268    1038

  Fragments arranged by size: 

                1684    1038     747     432     268
 
EcoO109I rG'GnC_Cy

Cuts at:   1308    1308 
   Size:       4169
 
EcoRI G'AATT_C

Cuts at:    892    1173     892 
   Size:        281    3888
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    643    2188     643 
   Size:       1545    2624
 
FspI TGC'GCA

Cuts at:   1950    1950 
   Size:       4169
 
HaeII r_GCGC'y

Cuts at:   1603    1851    2873    3852    1603 
   Size:        248    1022     979    1920

  Fragments arranged by size: 

                1920    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    942     974    2011    2041    2043    2073     942 
   Size:         32    1037      30       2      30    3038

  Fragments arranged by size: 

                3038    1037      32      30      30       2
 
HincII GTy'rAC

Cuts at:    412    1208     412 
   Size:        796    3373
 
KasI G'GCGC_C

Cuts at:   1599    1847    1599 
   Size:        248    3921
 
KpnI G_GTAC'C

Cuts at:    489    1189     489 
   Size:        700    3469
 
MluI A'CGCG_T

Cuts at:   3602    3602 
   Size:       4169
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    911    2840    3024     911 
   Size:       1929     184    2056

  Fragments arranged by size: 

                2056    1929     184
 
MscI TGG'CCA

Cuts at:    325    1930     325 
   Size:       1605    2564
 
MslI CAynn'nnrTG

Cuts at:    565     826    2158    2285     565 
   Size:        261    1332     127    2449

  Fragments arranged by size: 

                2449    1332     261     127
 
MspA1I CmG'CkG

Cuts at:    726    1280    1594    1954    2967    3212     726 
   Size:        554     314     360    1013     245    1683

  Fragments arranged by size: 

                1683    1013     554     360     314     245
 
NaeI GCC'GGC

Cuts at:   2350    2350 
   Size:       4169
 
NarI GG'CG_CC

Cuts at:   1600    1848    1600 
   Size:        248    3921
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       4169
 
NgoMIV G'CCGG_C

Cuts at:   2348    2348 
   Size:       4169
 
NspI r_CATG'y

Cuts at:    255    1222    1722    2629     255 
   Size:        967     500     907    1795

  Fragments arranged by size: 

                1795     967     907     500
 
PciI A'CATG_T

Cuts at:   2625    2625 
   Size:       4169
 
PfoI T'CCnGG_A

Cuts at:    887     887 
   Size:       4169
 
PmeI GTTT'AAAC

Cuts at:   1168    1168 
   Size:       4169
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3308    3340    3308 
   Size:         32    4137
 
PstI C_TGCA'G

Cuts at:    643    1216    1901     643 
   Size:        573     685    2911

  Fragments arranged by size: 

                2911     685     573
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4169
 
PvuII CAG'CTG

Cuts at:   1594    1954    1594 
   Size:        360    3809
 
RsrII CG'GwC_CG

Cuts at:   2364    2364 
   Size:       4169
 
SacI G_AGCT'C

Cuts at:   1183    1183 
   Size:       4169
 
SalI G'TCGA_C

Cuts at:   1206    1206 
   Size:       4169
 
SapI GCTCTTCn'nnn_

Cuts at:    286    2192    2402     286 
   Size:       1906     210    2053

  Fragments arranged by size: 

                2053    1906     210
 
SbfI CC_TGCA'GG

Cuts at:   1216    1216 
   Size:       4169
 
ScaI AGT'ACT

Cuts at:   3686    3686 
   Size:       4169
 
SfcI C'TryA_G

Cuts at:     33     639    1212    1897    2890    3081      33 
   Size:        606     573     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     606     573     191
 
SfoI GGC'GCC

Cuts at:   1601    1849    1601 
   Size:        248    3921
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4169
 
SmaI CCC'GGG

Cuts at:   1191    1191 
   Size:       4169
 
SmlI C'TyrA_G

Cuts at:   1324    2239    2731    2993    1324 
   Size:        915     492     262    2500

  Fragments arranged by size: 

                2500     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4169
 
SphI G_CATG'C

Cuts at:   1222    1722    1222 
   Size:        500    3669
 
StyI C'CwwG_G

Cuts at:    107    1303    1657     107 
   Size:       1196     354    2619

  Fragments arranged by size: 

                2619    1196     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    555    1752    1885    2344    2553     555 
   Size:       1197     133     459     209    2171

  Fragments arranged by size: 

                2171    1197     459     209     133
 
TatI w'GTAC_w

Cuts at:   3684    3684 
   Size:       4169
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3686    3718    3686 
   Size:         32    4137
 
Tth111I GACn'n_nGTC

Cuts at:   1966    1966 
   Size:       4169
 
XbaI T'CTAG_A

Cuts at:   1200    1200 
   Size:       4169
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    391     895    1664     391 
   Size:        504     769    2896

  Fragments arranged by size: 

                2896     769     504
 
XmaI C'CCGG_G

Cuts at:   1189    1189 
   Size:       4169
 
XmnI GAAnn'nnTTC

Cuts at:    626     682     883     626 
   Size:         56     201    3912

  Fragments arranged by size: 

                3912     201      56
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AleI
    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI
    BanII     BbeI     BbsI    BciVI     BclI    BglII     BlpI Bme1580I
     BmrI     BplI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BseYI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI   BstEII    BstYI
     BtgI    BtgZI     BtsI     ClaI     DraI     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI     FspI
    HaeII    Hin4I   HincII     KasI     KpnI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI     PciI
     PfoI     PmeI     PpiI     PstI     PvuI    PvuII    RsrII     SacI
     SalI     SapI     SbfI     ScaI     SfcI     SfoI     SgfI     SmaI
     SmlI     SpeI     SphI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AhdI     AlfI     AloI     ApaI     AscI
    AvrII     BaeI    BbvCI     BcgI    BfrBI     BglI    BmgBI     BmtI
     BpmI   Bpu10I    BsaXI    BseRI     BsgI    BsiWI     BsmI    BsrGI
   BssHII    BstXI  BstZ17I   Bsu36I    CspCI   DraIII    EcoNI    EcoRV
     FseI    FspAI  HindIII     HpaI     MfeI     NdeI     NheI     NotI
     NruI     NsiI     PacI     PasI    PflMI     PmlI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI    SacII    SanDI    SexAI     SfiI
    SgrAI    SnaBI     SrfI     SspI     StuI     SwaI     XhoI     ZraI
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