Restriction map for pF1KB9835
 (Circular) (Six-base) MAPSORT of: pF1KB9835.seq  Check: 1907  from: 1  to: 4163



pF1KB9835  4163 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:32  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    180     180 
   Size:       4163
 
AccI GT'mk_AC

Cuts at:    335    1201     335 
   Size:        866    3297
 
Acc65I G'GTAC_C

Cuts at:   1179    1179 
   Size:       4163
 
AclI AA'CG_TT

Cuts at:   4003    4003 
   Size:       4163
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    475    1988    2420    3167    3435     475 
   Size:       1513     432     747     268    1203

  Fragments arranged by size: 

                1513    1203     747     432     268
 
AflIII A'CryG_T

Cuts at:   1515    2619    3596    1515 
   Size:       1104     977    2082

  Fragments arranged by size: 

                2082    1104     977
 
AgeI A'CCGG_T

Cuts at:   2551    2551 
   Size:       4163
 
AleI CACnn'nnGTG

Cuts at:    132     132 
   Size:       4163
 
AlwNI CAG_nnn'CTG

Cuts at:    194    1468    3035     194 
   Size:       1274    1567    1322

  Fragments arranged by size: 

                1567    1322    1274
 
ApaI G_GGCC'C

Cuts at:    966     966 
   Size:       4163
 
ApaLI G'TGCA_C

Cuts at:   2933    2933 
   Size:       4163
 
ApoI r'AATT_y

Cuts at:    153    1038    1167    3516    3772     153 
   Size:        885     129    2349     256     544

  Fragments arranged by size: 

                2349     885     544     256     129
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4163
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4163
 
AvaI C'yCGr_G

Cuts at:   1183    1183 
   Size:       4163
 
BamHI G'GATC_C

Cuts at:    716    1188     716 
   Size:        472    3691
 
BanI G'GyrC_C

Cuts at:   1179    1593    1841    1876    1179 
   Size:        414     248      35    3466

  Fragments arranged by size: 

                3466     414     248      35
 
BanII G_rGCy'C

Cuts at:    966    1177    2207     966 
   Size:        211    1030    2922

  Fragments arranged by size: 

                2922    1030     211
 
BbeI G_GCGC'C

Cuts at:   1597    1845    1597 
   Size:        248    3915
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    936     970     936 
   Size:         34    4129
 
BciVI GTATCCnnnnn_n'

Cuts at:   2061    2822    4163    2061 
   Size:        761    1341    2061

  Fragments arranged by size: 

                2061    1341     761
 
BclI T'GATC_A

Cuts at:    233    1683     233 
   Size:       1450    2713
 
BfrBI ATG'CAT

Cuts at:   1074    1074 
   Size:       4163
 
BglII A'GATC_T

Cuts at:   1678    3715    1678 
   Size:       2037    2126
 
BlpI GC'TnA_GC

Cuts at:    262    1275     262 
   Size:       1013    3150
 
Bme1580I G_kGCm'C

Cuts at:    966    1788    1881    2937     966 
   Size:        822      93    1056    2192

  Fragments arranged by size: 

                2192    1056     822      93
 
BmgBI CAC'GTC

Cuts at:   1105    1105 
   Size:       4163
 
BmrI ACTGGGnnnn_n'

Cuts at:   1375    1456    1791    3935    1375 
   Size:         81     335    2144    1603

  Fragments arranged by size: 

                2144    1603     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2530    2530 
   Size:       4163
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1042    1339    2218    2710    3008    1042 
   Size:        297     879     492     298    2197

  Fragments arranged by size: 

                2197     879     492     298     297
 
BsaI GGTCTCn'nnnn_

Cuts at:    450    3867     450 
   Size:       3417     746
 
BsaAI yAC'GTr

Cuts at:   1516    1516 
   Size:       4163
 
BsaBI GATnn'nnATC

Cuts at:      6    1677    3714       6 
   Size:       1671    2037     455

  Fragments arranged by size: 

                2037    1671     455
 
BsaHI Gr'CG_yC

Cuts at:   1594    1842    2544    4040    1594 
   Size:        248     702    1496    1717

  Fragments arranged by size: 

                1717    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:   1352    1873    2551    2825    2972    3383    1352 
   Size:        521     678     274     147     411    2132

  Fragments arranged by size: 

                2132     678     521     411     274     147
 
BseYI C'CCAG_C

Cuts at:    270     587    1588    2346    2923    3419     270 
   Size:        317    1001     758     577     496    1014

  Fragments arranged by size: 

                1014    1001     758     577     496     317
 
BsiEI CG_ry'CG

Cuts at:    104     318     960    1705    1751    2959     104 
   Size:        214     642     745      46    1208    1308

  Fragments arranged by size: 

                1308    1208     745     642     214      46
 
BsiHKAI G_wGCw'C

Cuts at:   1177    1955    2145    2937    1177 
   Size:        778     190     792    2403

  Fragments arranged by size: 

                2403     792     778     190
 
Bsp1286I G_dGCh'C

Cuts at:    966    1177    1788    1881    1955    2145    2207    2937     966 
   Size:        211     611      93      74     190      62     730    2192

  Fragments arranged by size: 

                2192     730     611     211     190      93      74      62
            
 
BspEI T'CCGG_A

Cuts at:   3383    3383 
   Size:       4163
 
BspHI T'CATG_A

Cuts at:    908    3339     908 
   Size:       2431    1732
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    180     347    1213    1427    1729    2110     180 
   Size:        167     866     214     302     381    2233

  Fragments arranged by size: 

                2233     866     381     302     214     167
 
BsrBI CCG'CTC

Cuts at:     50     320    2455    2509    3993    4158      50 
   Size:        270    2135      54    1484     165      55

  Fragments arranged by size: 

                2135    1484     270     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2075    2075 
   Size:       4163
 
BsrFI r'CCGG_y

Cuts at:   2161    2342    2551    2161 
   Size:        181     209    3773

  Fragments arranged by size: 

                3773     209     181
 
BssSI C'ACGA_G

Cuts at:   2434    2792    2434 
   Size:        358    3805
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1540    1540 
   Size:       4163
 
BstBI TT'CG_AA

Cuts at:   2524    2524 
   Size:       4163
 
BstXI CCAn_nnnn'nTGG

Cuts at:    134     134 
   Size:       4163
 
BstYI r'GATC_y

Cuts at:      1     123     716    1061    1188    1655    1678    2013    2259 
   Size:        122     593     345     127     467      23     335     246

Cuts at:   2259    3271    3357    3369    3715       1 
   Size:       1012      86      12     346     449

  Fragments arranged by size: 

                1012     593     467     449     346     345     335     246
                 127     122      86      23      12
 
BtgI C'CryG_G

Cuts at:   1047    1047 
   Size:       4163
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    222    2113    2294    2457     222 
   Size:       1891     181     163    1928

  Fragments arranged by size: 

                1928    1891     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1406    1406 
   Size:       4163
 
DraI TTT'AAA

Cuts at:   1162    1162 
   Size:       4163
 
DrdI GACnn_nn'nnGTC

Cuts at:   1869    2727    1869 
   Size:        858    3305
 
EaeI y'GGCC_r

Cuts at:    315     356     518     957    1032    1748    1922    2313    2340 
   Size:         41     162     439      75     716     174     391      27

Cuts at:   2340    3685     315 
   Size:       1345     793

  Fragments arranged by size: 

                1345     793     716     439     391     174     162      75
                  41      27
 
EagI C'GGCC_G

Cuts at:    315     957    1748     315 
   Size:        642     791    2730

  Fragments arranged by size: 

                2730     791     642
 
EarI CTCTTCn'nnn_

Cuts at:   1152    2186    2396    1152 
   Size:       1034     210    2919

  Fragments arranged by size: 

                2919    1034     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    619     717    2371    2681    2827    3973     619 
   Size:         98    1654     310     146    1146     809

  Fragments arranged by size: 

                1654    1146     809     310     146      98
 
EcoICRI GAG'CTC

Cuts at:   1175    1175 
   Size:       4163
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    475    1988    2420    3167    3435     475 
   Size:       1513     432     747     268    1203

  Fragments arranged by size: 

                1513    1203     747     432     268
 
EcoNI CCTnn'n_nnAGG

Cuts at:    436     436 
   Size:       4163
 
EcoO109I rG'GnC_Cy

Cuts at:    675     914     963    1302     675 
   Size:        239      49     339    3536

  Fragments arranged by size: 

                3536     339     239      49
 
EcoRI G'AATT_C

Cuts at:   1167    1167 
   Size:       4163
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    576    1113    2182     576 
   Size:        537    1069    2557

  Fragments arranged by size: 

                2557    1069     537
 
FspI TGC'GCA

Cuts at:   1944    1944 
   Size:       4163
 
HaeII r_GCGC'y

Cuts at:    945    1597    1845    2867    3846     945 
   Size:        652     248    1022     979    1262

  Fragments arranged by size: 

                1262    1022     979     652     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    708     740    2005    2035    2037    2067     708 
   Size:         32    1265      30       2      30    2804

  Fragments arranged by size: 

                2804    1265      32      30      30       2
 
HincII GTy'rAC

Cuts at:    465    1202     465 
   Size:        737    3426
 
KasI G'GCGC_C

Cuts at:   1593    1841    1593 
   Size:        248    3915
 
KpnI G_GTAC'C

Cuts at:   1183    1183 
   Size:       4163
 
MluI A'CGCG_T

Cuts at:   3596    3596 
   Size:       4163
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1175    2834    3018    1175 
   Size:       1659     184    2320

  Fragments arranged by size: 

                2320    1659     184
 
MscI TGG'CCA

Cuts at:    520    1924     520 
   Size:       1404    2759
 
MslI CAynn'nnrTG

Cuts at:    132     163     432     502     907    2152    2279     132 
   Size:         31     269      70     405    1245     127    2016

  Fragments arranged by size: 

                2016    1245     405     269     127      70      31
 
MspA1I CmG'CkG

Cuts at:    750    1274    1588    1948    2961    3206     750 
   Size:        524     314     360    1013     245    1707

  Fragments arranged by size: 

                1707    1013     524     360     314     245
 
NaeI GCC'GGC

Cuts at:   2344    2344 
   Size:       4163
 
NarI GG'CG_CC

Cuts at:   1594    1842    1594 
   Size:        248    3915
 
NcoI C'CATG_G

Cuts at:   1047    1047 
   Size:       4163
 
NgoMIV G'CCGG_C

Cuts at:   2342    2342 
   Size:       4163
 
NsiI A_TGCA'T

Cuts at:   1076    1076 
   Size:       4163
 
NspI r_CATG'y

Cuts at:    168    1216    1716    2623     168 
   Size:       1048     500     907    1708

  Fragments arranged by size: 

                1708    1048     907     500
 
PciI A'CATG_T

Cuts at:   2619    2619 
   Size:       4163
 
PfoI T'CCnGG_A

Cuts at:    926     926 
   Size:       4163
 
PmeI GTTT'AAAC

Cuts at:   1162    1162 
   Size:       4163
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3302    3334    3302 
   Size:         32    4131
 
PpuMI rG'GwC_Cy

Cuts at:    675     914     675 
   Size:        239    3924
 
PspOMI G'GGCC_C

Cuts at:    962     962 
   Size:       4163
 
PstI C_TGCA'G

Cuts at:    177    1210    1895     177 
   Size:       1033     685    2445

  Fragments arranged by size: 

                2445    1033     685
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4163
 
PvuII CAG'CTG

Cuts at:   1588    1948    1588 
   Size:        360    3803
 
RsrII CG'GwC_CG

Cuts at:   2358    2358 
   Size:       4163
 
SacI G_AGCT'C

Cuts at:   1177    1177 
   Size:       4163
 
SalI G'TCGA_C

Cuts at:   1200    1200 
   Size:       4163
 
SapI GCTCTTCn'nnn_

Cuts at:   2186    2396    2186 
   Size:        210    3953
 
SbfI CC_TGCA'GG

Cuts at:   1210    1210 
   Size:       4163
 
ScaI AGT'ACT

Cuts at:   3680    3680 
   Size:       4163
 
SexAI A'CCwGG_T

Cuts at:    884     884 
   Size:       4163
 
SfcI C'TryA_G

Cuts at:     33     173     471    1206    1891    2884    3075      33 
   Size:        140     298     735     685     993     191    1121

  Fragments arranged by size: 

                1121     993     735     685     298     191     140
 
SfoI GGC'GCC

Cuts at:   1595    1843    1595 
   Size:        248    3915
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4163
 
SmaI CCC'GGG

Cuts at:   1185    1185 
   Size:       4163
 
SmlI C'TyrA_G

Cuts at:   1057    1318    2233    2725    2987    1057 
   Size:        261     915     492     262    2233

  Fragments arranged by size: 

                2233     915     492     262     261
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4163
 
SphI G_CATG'C

Cuts at:    168    1216    1716     168 
   Size:       1048     500    2615

  Fragments arranged by size: 

                2615    1048     500
 
SspI AAT'ATT

Cuts at:    117     117 
   Size:       4163
 
StyI C'CwwG_G

Cuts at:    353     401    1047    1297    1651     353 
   Size:         48     646     250     354    2865

  Fragments arranged by size: 

                2865     646     354     250      48
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1746    1879    2338    2547    1746 
   Size:        133     459     209    3362

  Fragments arranged by size: 

                3362     459     209     133
 
TatI w'GTAC_w

Cuts at:   3678    3678 
   Size:       4163
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3680    3712    3680 
   Size:         32    4131
 
Tth111I GACn'n_nGTC

Cuts at:   1960    1960 
   Size:       4163
 
XbaI T'CTAG_A

Cuts at:   1194    1194 
   Size:       4163
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    981    1658     981 
   Size:        677    3486
 
XmaI C'CCGG_G

Cuts at:   1183    1183 
   Size:       4163
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AleI
    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI
     BanI    BanII     BbeI     BcgI    BciVI     BclI    BfrBI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BplI    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BseYI    BsiEI  BsiHKAI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI
    BstXI    BstYI     BtgI    BtgZI     BtsI     DraI     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
     FalI     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV
     NsiI     NspI     PciI     PfoI     PmeI     PpiI    PpuMI   PspOMI
     PstI     PvuI    PvuII    RsrII     SacI     SalI     SapI     SbfI
     ScaI    SexAI     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI
     SphI     SspI     StyI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AhdI     AlfI     AloI     AscI    AvrII
     BaeI     BbsI    BbvCI     BglI     BmtI     BpmI   Bpu10I    BsaXI
    BseRI     BsgI    BsiWI     BsmI    BsmBI    BsrGI   BssHII   BstEII
  BstZ17I   Bsu36I     ClaI    CspCI   DraIII    EcoRV     FseI    FspAI
  HindIII     HpaI     MfeI     NdeI     NheI     NotI     NruI     PacI
     PasI    PflMI     PmlI    PshAI     PsiI    PspXI     PsrI    SacII
    SanDI     SfiI    SgrAI    SnaBI     SrfI     StuI     SwaI     XhoI
     XmnI     ZraI
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