Restriction map for pF1KB9834
##################################
# NO CUT ENZYMES (  107 enzymes) #
##################################

Acc65I      AccI        AclI        AcsI        AflII       
AflIII      AgeI        AhdI        ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AviII       BamHI       BbrPI       BbvCI       
BcgI        BciVI       BfrI        BsaAI       BsaBI       
BseRI       BsgI        BsiEI       BsiWI       BsmBI       
BsmI        BspDI       BspHI       BspLU11I    BsrDI       
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstZ17I     ClaI        CpoI        DraI        
DraIII      DrdI        EagI        Eam1105I    EclXI       
Eco52I      EcoO65I     EcoRI       EcoRV       EcoT22I     
FseI        FspI        HincII      HindII      HindIII     
HpaI        KpnI        MamI        MfeI        MluI        
MunI        NcoI        NdeI        NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PvuI        PvuII       RcaI        RsrII       SalI        
SbfI        SexAI       SfiI        SfuI        SgrAI       
SnaBI       SpeI        SphI        Sse8387I    SspI        
StuI        SwaI        TfiI        Tth111I     XbaI        
XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   58 enzymes) #
###################################

AatII         943  GACGTC               GACGT^C
AccIII         32  TCCGGA               T^CCGGA
AlwI         1008  GGATC                GGATC(4/5)
AlwNI         190  CAGAGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          472  CCTGAGG              CC^T[ACGT]AGG
Aor51HI       953  AGCGCT               AGC^GCT
ApaI          125  GGGCCC               GGGCC^C
AvrII         399  CCTAGG               C^CTAGG
BalI          251  TGGCCA               TGG^CCA
BbsI          907  GTCTTC               GAAGAC(2/6)
BclI          623  TGATCA               T^GATCA
BglI          138  GCCGCGCCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         681  AGATCT               A^GATCT
BlnI          399  CCTAGG               C^CTAGG
BlpI          313  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      313  GCTGAGC              GC^T[ACGT]AGC
BpuAI         907  GTCTTC               GAAGAC(2/6)
BsaI          963  GAGACC               GGTCTC(1/5)
BsaWI          32  TCCGGA               [AT]^CCGG[AT]
BseAI          32  TCCGGA               T^CCGGA
Bsp1407I      221  TGTACA               T^GTACA
BspEI          32  TCCGGA               T^CCGGA
BsrBI         844  GAGCGG               CCGCTC(-3/-3)
BsrGI         221  TGTACA               T^GTACA
BssHII        989  GCGCGC               G^CGCGC
BstXI         407  CCATCATCATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        472  CCTGAGG              CC^T[ACGT]AGG
CelII         313  GCTGAGC              GC^T[ACGT]AGC
DraII         125  GGGCCCC              [GA]G^G[ACGT]CC[CT]
Eco47III      953  AGCGCT               AGC^GCT
Eco81I        472  CCTGAGG              CC^T[ACGT]AGG
EcoNI         712  CCTCTAGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      125  GGGCCCC              [GA]G^G[ACGT]CC[CT]
FbaI          623  TGATCA               T^GATCA
HinfI         916  GACTC                G^A[ACGT]TC
HphI         1013  TCACC                GGTGA(8/7)
MluNI         251  TGGCCA               TGG^CCA
MroI           32  TCCGGA               T^CCGGA
MscI          251  TGGCCA               TGG^CCA
MseI          517  TTAA                 T^TAA
MslI          408  CATCATCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NlaIII        414  CATG                 CATG^
PflMI         161  CCATTGACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          916  GACTC                GAGTC(4/5)
SapI          567  GCTCTTC              GCTCTTC(1/4)
ScaI          539  AGTACT               AGT^ACT
SfaNI         292  GCATC                GCATC(5/9)
SmaI          925  CCCGGG               CCC^GGG
SmlI          535  CTCAAG               C^T[CT][GA]AG
SspBI         221  TGTACA               T^GTACA
TaqI          911  TCGA                 T^CGA
Tru9I         517  TTAA                 T^TAA
Tsp509I       648  AATT                 ^AATT
TthHB8I       911  TCGA                 T^CGA
Van91I        161  CCATTGACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          444  CCAAGTCATCTATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI         925  CCCGGG               C^CCGGG
XmaI          925  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   34 enzymes) #
###################################

Alw44I        273  GTGCAC               G^TGCAC
              592  GTGCAC              
ApaLI         273  GTGCAC               G^TGCAC
              592  GTGCAC              
AvaI          122  CTCGGG               C^[CT]CG[GA]G
              925  CCCGGG              
BanI          457  GGCGCC               G^G[CT][GA]CC
              828  GGCGCC              
BbeI          457  GGCGCC               GGCGC^C
              828  GGCGCC              
BsmAI         963  GAGAC                GTCTC(1/5)
              533  GTCTC               
BsoBI         122  CTCGGG               C^[CT]CG[GA]G
              925  CCCGGG              
BsrFI         143  GCCGGC               [GA]^CCGG[CT]
              463  GCCGGC              
BsrI          167  ACTGG                ACTGG(1/-1)
              505  ACTGG               
BstF5I        948  CATCC                GGATG(2/0)
              636  GGATG               
BstNI          12  CCTGG                CC^[AT]GG
              152  CCTGG               
BstYI         681  AGATCT               [GA]^GATC[CT]
             1008  GGATCT              
Cfr10I        143  GCCGGC               [GA]^CCGG[CT]
              463  GCCGGC              
DsaI          130  CCGCGG               C^C[GA][CT]GG
              237  CCGCGG              
EaeI          251  TGGCCA               [CT]^GGCC[GA]
              465  CGGCCA              
EarI          116  CTCTTC               CTCTTC(1/4)
              568  CTCTTC              
EcoRII         12  CCTGG                ^CC[AT]GG
              152  CCTGG               
EcoT14I       399  CCTAGG               C^C[AT][AT]GG
              936  CCTTGG              
FokI          948  CATCC                GGATG(9/13)
              636  GGATG               
KasI          457  GGCGCC               G^GCGCC
              828  GGCGCC              
Ksp632I       116  CTCTTC               CTCTTC(1/4)
              568  CTCTTC              
KspI          130  CCGCGG               CCGC^GG
              237  CCGCGG              
MflI          681  AGATCT               [GA]^GATC[CT]
             1008  GGATCT              
MvaI           12  CCTGG                CC^[AT]GG
              152  CCTGG               
NaeI          143  GCCGGC               GCC^GGC
              463  GCCGGC              
NarI          457  GGCGCC               GG^CGCC
              828  GGCGCC              
NgoMIV        143  GCCGGC               G^CCGGC
              463  GCCGGC              
PstI           69  CTGCAG               CTGCA^G
              187  CTGCAG              
SacI          316  GAGCTC               GAGCT^C
              745  GAGCTC              
SacII         130  CCGCGG               CCGC^GG
              237  CCGCGG              
SfcI           69  CTGCAG               C^T[GA][CT]AG
              187  CTGCAG              
SfoI          457  GGCGCC               GGC^GCC
              828  GGCGCC              
StyI          399  CCTAGG               C^C[AT][AT]GG
              936  CCTTGG              
XhoII         681  AGATCT               [GA]^GATC[CT]
             1008  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   59 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AspHI       AvaII       BanII       BbvI        BcnI        
BfaI        BmyI        BpmI        BsaHI       BsaJI       
BsiHKAI     BsiYI       BslI        BsmFI       Bsp1286I    
BspMI       BssKI       BstUI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       FauI        Fnu4HI      
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HpaII       ItaI        MaeI        
MaeII       MaeIII      MboI        MboII       MnlI        
MspA1I      MspI        MvnI        MwoI        NciI        
NdeII       NlaIV       RsaI        Sau3AI      Sau96I      
ScrFI       TseI        Tsp45I      TspRI       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com