Restriction map for pF1KB9834
 (Circular) (Six-base) MAPSORT of: pF1KB9834.seq  Check: 1156  from: 1  to: 4151



pF1KB9834  4151 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:32  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    273     273 
   Size:       4151
 
AatII G_ACGT'C

Cuts at:   1055    1055 
   Size:       4151
 
AccI GT'mk_AC

Cuts at:   1189    1189 
   Size:       4151
 
Acc65I G'GTAC_C

Cuts at:   1167    1167 
   Size:       4151
 
AclI AA'CG_TT

Cuts at:   3991    3991 
   Size:       4151
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    733    1976    2408    3155    3423     733 
   Size:       1243     432     747     268    1461

  Fragments arranged by size: 

                1461    1243     747     432     268
 
AfeI AGC'GCT

Cuts at:   1063    1063 
   Size:       4151
 
AflIII A'CryG_T

Cuts at:   1503    2607    3584    1503 
   Size:       1104     977    2070

  Fragments arranged by size: 

                2070    1104     977
 
AgeI A'CCGG_T

Cuts at:   2539    2539 
   Size:       4151
 
AlwNI CAG_nnn'CTG

Cuts at:    303    1456    3023     303 
   Size:       1153    1567    1431

  Fragments arranged by size: 

                1567    1431    1153
 
ApaI G_GGCC'C

Cuts at:    237     237 
   Size:       4151
 
ApaLI G'TGCA_C

Cuts at:    381     700    2921     381 
   Size:        319    2221    1611

  Fragments arranged by size: 

                2221    1611     319
 
ApoI r'AATT_y

Cuts at:   1155    3504    3760    1155 
   Size:       2349     256    1546

  Fragments arranged by size: 

                2349    1546     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4151
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4151
 
AvaI C'yCGr_G

Cuts at:    230    1033    1171     230 
   Size:        803     138    3210

  Fragments arranged by size: 

                3210     803     138
 
AvrII C'CTAG_G

Cuts at:    507     507 
   Size:       4151
 
BamHI G'GATC_C

Cuts at:   1176    1176 
   Size:       4151
 
BanI G'GyrC_C

Cuts at:    565     936    1167    1581    1829    1864     565 
   Size:        371     231     414     248      35    2852

  Fragments arranged by size: 

                2852     414     371     248     231      35
 
BanII G_rGCy'C

Cuts at:    237     428     857    1165    2195     237 
   Size:        191     429     308    1030    2193

  Fragments arranged by size: 

                2193    1030     429     308     191
 
BbeI G_GCGC'C

Cuts at:    569     940    1585    1833     569 
   Size:        371     645     248    2887

  Fragments arranged by size: 

                2887     645     371     248
 
BbsI GAAGACnn'nnnn_

Cuts at:   1008    1008 
   Size:       4151
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:     90     124      90 
   Size:         34    4117
 
BciVI GTATCCnnnnn_n'

Cuts at:   2049    2810    4151    2049 
   Size:        761    1341    2049

  Fragments arranged by size: 

                2049    1341     761
 
BclI T'GATC_A

Cuts at:    731    1671     731 
   Size:        940    3211
 
BglI GCCn_nnn'nGGC

Cuts at:    252     252 
   Size:       4151
 
BglII A'GATC_T

Cuts at:    789    1666    3703     789 
   Size:        877    2037    1237

  Fragments arranged by size: 

                2037    1237     877
 
BlpI GC'TnA_GC

Cuts at:    422    1263     422 
   Size:        841    3310
 
Bme1580I G_kGCm'C

Cuts at:    237     385     704    1776    1869    2925     237 
   Size:        148     319    1072      93    1056    1463

  Fragments arranged by size: 

                1463    1072    1056     319     148      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    285    1363    1444    1779    3923     285 
   Size:       1078      81     335    2144     513

  Fragments arranged by size: 

                2144    1078     513     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2518    2518 
   Size:       4151
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    332     635    1089     332 
   Size:        303     454    3394

  Fragments arranged by size: 

                3394     454     303
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    628    1327    2206    2698    2996     628 
   Size:        699     879     492     298    1783

  Fragments arranged by size: 

                1783     879     699     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   1065    3855    1065 
   Size:       2790    1361
 
BsaAI yAC'GTr

Cuts at:   1504    1504 
   Size:       4151
 
BsaBI GATnn'nnATC

Cuts at:      6    1665    3702       6 
   Size:       1659    2037     455

  Fragments arranged by size: 

                2037    1659     455
 
BsaHI Gr'CG_yC

Cuts at:    566     877     937    1052    1582    1830    2532    4028     566 
   Size:        311      60     115     530     248     702    1496     689

  Fragments arranged by size: 

                1496     702     689     530     311     248     115      60
            
 
BsaWI w'CCGG_w

Cuts at:    140    1340    1861    2539    2813    2960    3371     140 
   Size:       1200     521     678     274     147     411     920

  Fragments arranged by size: 

                1200     920     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    271     301     857     887     271 
   Size:         30     556      30    3535

  Fragments arranged by size: 

                3535     556      30      30
 
BseYI C'CCAG_C

Cuts at:    303    1075    1576    2334    2911    3407     303 
   Size:        772     501     758     577     496    1047

  Fragments arranged by size: 

                1047     772     758     577     501     496
 
BsiEI CG_ry'CG

Cuts at:    104    1693    1739    2947     104 
   Size:       1589      46    1208    1308

  Fragments arranged by size: 

                1589    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    268     385     428     704     857    1165    1943    2133    2925 
   Size:        117      43     276     153     308     778     190     792

Cuts at:   2925     268 
   Size:       1494

  Fragments arranged by size: 

                1494     792     778     308     276     190     153     117
                  43
 
Bsp1286I G_dGCh'C

Cuts at:    237     268     385     428     704     857    1165    1776    1869 
   Size:         31     117      43     276     153     308     611      93

Cuts at:   1869    1943    2133    2195    2925     237 
   Size:         74     190      62     730    1463

  Fragments arranged by size: 

                1463     730     611     308     276     190     153     117
                  93      74      62      43      31
 
BspEI T'CCGG_A

Cuts at:    140    3371     140 
   Size:       3231     920
 
BspHI T'CATG_A

Cuts at:   3327    3327 
   Size:       4151
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    207     273     998    1201    1415    1717    2098     207 
   Size:         66     725     203     214     302     381    2260

  Fragments arranged by size: 

                2260     725     381     302     214     203      66
 
BsrBI CCG'CTC

Cuts at:     50     954    2443    2497    3981    4146      50 
   Size:        904    1489      54    1484     165      55

  Fragments arranged by size: 

                1489    1484     904     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2063    2063 
   Size:       4151
 
BsrFI r'CCGG_y

Cuts at:    251     571    2149    2330    2539     251 
   Size:        320    1578     181     209    1863

  Fragments arranged by size: 

                1863    1578     320     209     181
 
BsrGI T'GTAC_A

Cuts at:    329     329 
   Size:       4151
 
BssHII G'CGCG_C

Cuts at:   1097    1097 
   Size:       4151
 
BssSI C'ACGA_G

Cuts at:   2422    2780    2422 
   Size:        358    3793
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1528    1528 
   Size:       4151
 
BstBI TT'CG_AA

Cuts at:   2512    2512 
   Size:       4151
 
BstXI CCAn_nnnn'nTGG

Cuts at:    522     522 
   Size:       4151
 
BstYI r'GATC_y

Cuts at:      1     789    1116    1176    1643    1666    2001    2247    3259 
   Size:        788     327      60     467      23     335     246    1012

Cuts at:   3259    3345    3357    3703       1 
   Size:         86      12     346     449

  Fragments arranged by size: 

                1012     788     467     449     346     335     327     246
                  86      60      23      12
 
Bsu36I CC'TnA_GG

Cuts at:    581     581 
   Size:       4151
 
BtgI C'CryG_G

Cuts at:    238     345     238 
   Size:        107    4044
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2101    2282    2445    2101 
   Size:        181     163    3807

  Fragments arranged by size: 

                3807     181     163
 
BtsI GCAGTG_nn'

Cuts at:    603    1394     603 
   Size:        791    3360
 
DraI TTT'AAA

Cuts at:   1150    1150 
   Size:       4151
 
DrdI GACnn_nn'nnGTC

Cuts at:   1857    2715    1857 
   Size:        858    3293
 
EaeI y'GGCC_r

Cuts at:    359     573    1736    1910    2301    2328    3673     359 
   Size:        214    1163     174     391      27    1345     837

  Fragments arranged by size: 

                1345    1163     837     391     214     174      27
 
EagI C'GGCC_G

Cuts at:   1736    1736 
   Size:       4151
 
EarI CTCTTCn'nnn_

Cuts at:    230     682    2174    2384     230 
   Size:        452    1492     210    1997

  Fragments arranged by size: 

                1997    1492     452     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    423    2359    2669    2815    3961     423 
   Size:       1936     310     146    1146     613

  Fragments arranged by size: 

                1936    1146     613     310     146
 
EcoICRI GAG'CTC

Cuts at:    426     855    1163     426 
   Size:        429     308    3414

  Fragments arranged by size: 

                3414     429     308
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    332     635     733    1089    1976    2408    3155    3423     332 
   Size:        303      98     356     887     432     747     268    1060

  Fragments arranged by size: 

                1060     887     747     432     356     303     268      98
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:    824     824 
   Size:       4151
 
EcoO109I rG'GnC_Cy

Cuts at:    234    1290     234 
   Size:       1056    3095
 
EcoRI G'AATT_C

Cuts at:   1155    1155 
   Size:       4151
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2170    2170 
   Size:       4151
 
FspI TGC'GCA

Cuts at:   1932    1932 
   Size:       4151
 
HaeII r_GCGC'y

Cuts at:    569     940    1044    1065    1585    1833    2855    3834     569 
   Size:        371     104      21     520     248    1022     979     886

  Fragments arranged by size: 

                1022     979     886     520     371     248     104      21
            
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1108    1140    1993    2023    2025    2055    1108 
   Size:         32     853      30       2      30    3204

  Fragments arranged by size: 

                3204     853      32      30      30       2
 
HincII GTy'rAC

Cuts at:   1190    1190 
   Size:       4151
 
KasI G'GCGC_C

Cuts at:    565     936    1581    1829     565 
   Size:        371     645     248    2887

  Fragments arranged by size: 

                2887     645     371     248
 
KpnI G_GTAC'C

Cuts at:   1171    1171 
   Size:       4151
 
MluI A'CGCG_T

Cuts at:   3584    3584 
   Size:       4151
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    764    2822    3006     764 
   Size:       2058     184    1909

  Fragments arranged by size: 

                2058    1909     184
 
MscI TGG'CCA

Cuts at:    361    1912     361 
   Size:       1551    2600
 
MslI CAynn'nnrTG

Cuts at:    520    2140    2267     520 
   Size:       1620     127    2404

  Fragments arranged by size: 

                2404    1620     127
 
MspA1I CmG'CkG

Cuts at:    240     347    1009    1262    1576    1936    2949    3194     240 
   Size:        107     662     253     314     360    1013     245    1197

  Fragments arranged by size: 

                1197    1013     662     360     314     253     245     107
            
 
NaeI GCC'GGC

Cuts at:    253     573    2332     253 
   Size:        320    1759    2072

  Fragments arranged by size: 

                2072    1759     320
 
NarI GG'CG_CC

Cuts at:    566     937    1582    1830     566 
   Size:        371     645     248    2887

  Fragments arranged by size: 

                2887     645     371     248
 
NgoMIV G'CCGG_C

Cuts at:    251     571    2330     251 
   Size:        320    1759    2072

  Fragments arranged by size: 

                2072    1759     320
 
NspI r_CATG'y

Cuts at:   1204    1704    2611    1204 
   Size:        500     907    2744

  Fragments arranged by size: 

                2744     907     500
 
PciI A'CATG_T

Cuts at:   2607    2607 
   Size:       4151
 
PflMI CCAn_nnn'nTGG

Cuts at:    275     275 
   Size:       4151
 
PmeI GTTT'AAAC

Cuts at:   1150    1150 
   Size:       4151
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3290    3322    3290 
   Size:         32    4119
 
PspOMI G'GGCC_C

Cuts at:    233     233 
   Size:       4151
 
PstI C_TGCA'G

Cuts at:    181     299    1198    1883     181 
   Size:        118     899     685    2449

  Fragments arranged by size: 

                2449     899     685     118
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4151
 
PvuII CAG'CTG

Cuts at:   1576    1936    1576 
   Size:        360    3791
 
RsrII CG'GwC_CG

Cuts at:   2346    2346 
   Size:       4151
 
SacI G_AGCT'C

Cuts at:    428     857    1165     428 
   Size:        429     308    3414

  Fragments arranged by size: 

                3414     429     308
 
SacII CC_GC'GG

Cuts at:    241     348     241 
   Size:        107    4044
 
SalI G'TCGA_C

Cuts at:   1188    1188 
   Size:       4151
 
SapI GCTCTTCn'nnn_

Cuts at:    682    2174    2384     682 
   Size:       1492     210    2449

  Fragments arranged by size: 

                2449    1492     210
 
SbfI CC_TGCA'GG

Cuts at:   1198    1198 
   Size:       4151
 
ScaI AGT'ACT

Cuts at:    649    3668     649 
   Size:       3019    1132
 
SfcI C'TryA_G

Cuts at:     33     177     295    1194    1879    2872    3063      33 
   Size:        144     118     899     685     993     191    1121

  Fragments arranged by size: 

                1121     993     899     685     191     144     118
 
SfoI GGC'GCC

Cuts at:    567     938    1583    1831     567 
   Size:        371     645     248    2887

  Fragments arranged by size: 

                2887     645     371     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4151
 
SmaI CCC'GGG

Cuts at:   1035    1173    1035 
   Size:        138    4013
 
SmlI C'TyrA_G

Cuts at:    643    1306    2221    2713    2975     643 
   Size:        663     915     492     262    1819

  Fragments arranged by size: 

                1819     915     663     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4151
 
SphI G_CATG'C

Cuts at:   1204    1704    1204 
   Size:        500    3651
 
StyI C'CwwG_G

Cuts at:    507    1044    1285    1639     507 
   Size:        537     241     354    3019

  Fragments arranged by size: 

                3019     537     354     241
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    853    1734    1867    2326    2535     853 
   Size:        881     133     459     209    2469

  Fragments arranged by size: 

                2469     881     459     209     133
 
TatI w'GTAC_w

Cuts at:    329     647    3666     329 
   Size:        318    3019     814

  Fragments arranged by size: 

                3019     814     318
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    271     303     857     889    3668    3700     271 
   Size:         32     554      32    2779      32     722

  Fragments arranged by size: 

                2779     722     554      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1948    1948 
   Size:       4151
 
XbaI T'CTAG_A

Cuts at:   1182    1182 
   Size:       4151
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    559    1646     559 
   Size:       1087    3064
 
XmaI C'CCGG_G

Cuts at:   1033    1171    1033 
   Size:        138    4013
 
ZraI GAC'GTC

Cuts at:   1053    1053 
   Size:       4151
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI   AflIII
     AgeI    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI
    AvrII    BamHI     BanI    BanII     BbeI     BbsI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I     BmrI     BplI     BpmI
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI
    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI    BstXI    BstYI
   Bsu36I     BtgI    BtgZI     BtsI     DraI     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI
     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI   NgoMIV     NspI     PciI
    PflMI     PmeI     PpiI   PspOMI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI     SapI     SbfI     ScaI     SfcI     SfoI
     SgfI     SmaI     SmlI     SpeI     SphI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XmaI     ZraI

 Enzymes that do not cut: 

    AflII     AhdI     AleI     AlfI     AloI     AscI     BaeI    BbvCI
    BfrBI    BmgBI     BmtI   Bpu10I    BseRI     BsgI    BsiWI     BsmI
    BsmBI   BstEII  BstZ17I     ClaI    CspCI   DraIII    EcoRV     FseI
    FspAI  HindIII     HpaI     MfeI     NcoI     NdeI     NheI     NotI
     NruI     NsiI     PacI     PasI     PfoI     PmlI    PpuMI    PshAI
     PsiI    PspXI     PsrI    SanDI    SexAI     SfiI    SgrAI    SnaBI
     SrfI     SspI     StuI     SwaI     XhoI     XmnI
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