Restriction map for pF1KB9834
 (Linear) (Six-base) MAPSORT of: KIBB9834.seq  Check: 6908  from: 1  to: 1038



KIBB9834.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:12  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     165    1038 
   Size:        165     873
 
AatII G_ACGT'C

Cuts at:      0     947    1038 
   Size:        947      91
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     625    1038 
   Size:        625     413
 
AfeI AGC'GCT

Cuts at:      0     955    1038 
   Size:        955      83
 
AlwNI CAG_nnn'CTG

Cuts at:      0     195    1038 
   Size:        195     843
 
ApaI G_GGCC'C

Cuts at:      0     129    1038 
   Size:        129     909
 
ApaLI G'TGCA_C

Cuts at:      0     273     592    1038 
   Size:        273     319     446

  Fragments arranged by size: 

                 446     319     273
 
AvaI C'yCGr_G

Cuts at:      0     122     925    1038 
   Size:        122     803     113

  Fragments arranged by size: 

                 803     122     113
 
AvrII C'CTAG_G

Cuts at:      0     399    1038 
   Size:        399     639
 
BanI G'GyrC_C

Cuts at:      0     457     828    1038 
   Size:        457     371     210

  Fragments arranged by size: 

                 457     371     210
 
BanII G_rGCy'C

Cuts at:      0     129     320     749    1038 
   Size:        129     191     429     289

  Fragments arranged by size: 

                 429     289     191     129
 
BbeI G_GCGC'C

Cuts at:      0     461     832    1038 
   Size:        461     371     206

  Fragments arranged by size: 

                 461     371     206
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     900    1038 
   Size:        900     138
 
BclI T'GATC_A

Cuts at:      0     623    1038 
   Size:        623     415
 
BglI GCCn_nnn'nGGC

Cuts at:      0     144    1038 
   Size:        144     894
 
BglII A'GATC_T

Cuts at:      0     681    1038 
   Size:        681     357
 
BlpI GC'TnA_GC

Cuts at:      0     314    1038 
   Size:        314     724
 
Bme1580I G_kGCm'C

Cuts at:      0     129     277     596    1038 
   Size:        129     148     319     442

  Fragments arranged by size: 

                 442     319     148     129
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     177    1038 
   Size:        177     861
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     224     527     981    1038 
   Size:        224     303     454      57

  Fragments arranged by size: 

                 454     303     224      57
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     520    1038 
   Size:        520     518
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     957    1038 
   Size:        957      81
 
BsaHI Gr'CG_yC

Cuts at:      0     458     769     829     944    1038 
   Size:        458     311      60     115      94

  Fragments arranged by size: 

                 458     311     115      94      60
 
BsaWI w'CCGG_w

Cuts at:      0      32    1038 
   Size:         32    1006
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     163     193     749     779    1038 
   Size:        163      30     556      30     259

  Fragments arranged by size: 

                 556     259     163      30      30
 
BseYI C'CCAG_C

Cuts at:      0     195     967    1038 
   Size:        195     772      71

  Fragments arranged by size: 

                 772     195      71
 
BsiHKAI G_wGCw'C

Cuts at:      0     160     277     320     596     749    1038 
   Size:        160     117      43     276     153     289

  Fragments arranged by size: 

                 289     276     160     153     117      43
 
Bsp1286I G_dGCh'C

Cuts at:      0     129     160     277     320     596     749    1038 
   Size:        129      31     117      43     276     153     289

  Fragments arranged by size: 

                 289     276     153     129     117      43      31
 
BspEI T'CCGG_A

Cuts at:      0      32    1038 
   Size:         32    1006
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      99     165     890    1038 
   Size:         99      66     725     148

  Fragments arranged by size: 

                 725     148      99      66
 
BsrBI CCG'CTC

Cuts at:      0     846    1038 
   Size:        846     192
 
BsrFI r'CCGG_y

Cuts at:      0     143     463    1038 
   Size:        143     320     575

  Fragments arranged by size: 

                 575     320     143
 
BsrGI T'GTAC_A

Cuts at:      0     221    1038 
   Size:        221     817
 
BssHII G'CGCG_C

Cuts at:      0     989    1038 
   Size:        989      49
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     414    1038 
   Size:        414     624
 
BstYI r'GATC_y

Cuts at:      0     681    1008    1038 
   Size:        681     327      30

  Fragments arranged by size: 

                 681     327      30
 
Bsu36I CC'TnA_GG

Cuts at:      0     473    1038 
   Size:        473     565
 
BtgI C'CryG_G

Cuts at:      0     130     237    1038 
   Size:        130     107     801

  Fragments arranged by size: 

                 801     130     107
 
BtsI GCAGTG_nn'

Cuts at:      0     495    1038 
   Size:        495     543
 
EaeI y'GGCC_r

Cuts at:      0     251     465    1038 
   Size:        251     214     573

  Fragments arranged by size: 

                 573     251     214
 
EarI CTCTTCn'nnn_

Cuts at:      0     122     574    1038 
   Size:        122     452     464

  Fragments arranged by size: 

                 464     452     122
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     315    1038 
   Size:        315     723
 
EcoICRI GAG'CTC

Cuts at:      0     318     747    1038 
   Size:        318     429     291

  Fragments arranged by size: 

                 429     318     291
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     224     527     625     981    1038 
   Size:        224     303      98     356      57

  Fragments arranged by size: 

                 356     303     224      98      57
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     716    1038 
   Size:        716     322
 
EcoO109I rG'GnC_Cy

Cuts at:      0     126    1038 
   Size:        126     912
 
HaeII r_GCGC'y

Cuts at:      0     461     832     936     957    1038 
   Size:        461     371     104      21      81

  Fragments arranged by size: 

                 461     371     104      81      21
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1000    1032    1038 
   Size:       1000      32       6

  Fragments arranged by size: 

                1000      32       6
 
KasI G'GCGC_C

Cuts at:      0     457     828    1038 
   Size:        457     371     210

  Fragments arranged by size: 

                 457     371     210
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     656    1038 
   Size:        656     382
 
MscI TGG'CCA

Cuts at:      0     253    1038 
   Size:        253     785
 
MslI CAynn'nnrTG

Cuts at:      0     412    1038 
   Size:        412     626
 
MspA1I CmG'CkG

Cuts at:      0     132     239     901    1038 
   Size:        132     107     662     137

  Fragments arranged by size: 

                 662     137     132     107
 
NaeI GCC'GGC

Cuts at:      0     145     465    1038 
   Size:        145     320     573

  Fragments arranged by size: 

                 573     320     145
 
NarI GG'CG_CC

Cuts at:      0     458     829    1038 
   Size:        458     371     209

  Fragments arranged by size: 

                 458     371     209
 
NgoMIV G'CCGG_C

Cuts at:      0     143     463    1038 
   Size:        143     320     575

  Fragments arranged by size: 

                 575     320     143
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     167    1038 
   Size:        167     871
 
PspOMI G'GGCC_C

Cuts at:      0     125    1038 
   Size:        125     913
 
PstI C_TGCA'G

Cuts at:      0      73     191    1038 
   Size:         73     118     847

  Fragments arranged by size: 

                 847     118      73
 
SacI G_AGCT'C

Cuts at:      0     320     749    1038 
   Size:        320     429     289

  Fragments arranged by size: 

                 429     320     289
 
SacII CC_GC'GG

Cuts at:      0     133     240    1038 
   Size:        133     107     798

  Fragments arranged by size: 

                 798     133     107
 
SapI GCTCTTCn'nnn_

Cuts at:      0     574    1038 
   Size:        574     464
 
ScaI AGT'ACT

Cuts at:      0     541    1038 
   Size:        541     497
 
SfcI C'TryA_G

Cuts at:      0      69     187    1038 
   Size:         69     118     851

  Fragments arranged by size: 

                 851     118      69
 
SfoI GGC'GCC

Cuts at:      0     459     830    1038 
   Size:        459     371     208

  Fragments arranged by size: 

                 459     371     208
 
SmaI CCC'GGG

Cuts at:      0     927    1038 
   Size:        927     111
 
SmlI C'TyrA_G

Cuts at:      0     535    1038 
   Size:        535     503
 
StyI C'CwwG_G

Cuts at:      0     399     936    1038 
   Size:        399     537     102

  Fragments arranged by size: 

                 537     399     102
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     745    1038 
   Size:        745     293
 
TatI w'GTAC_w

Cuts at:      0     221     539    1038 
   Size:        221     318     499

  Fragments arranged by size: 

                 499     318     221
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     163     195     749     781    1038 
   Size:        163      32     554      32     257

  Fragments arranged by size: 

                 554     257     163      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     451    1038 
   Size:        451     587
 
XmaI C'CCGG_G

Cuts at:      0     925    1038 
   Size:        925     113
 
ZraI GAC'GTC

Cuts at:      0     945    1038 
   Size:        945      93
 

 Enzymes that do cut:

     AarI    AatII     AcuI     AfeI    AlwNI     ApaI    ApaLI     AvaI
    AvrII     BanI    BanII     BbeI     BbsI     BclI     BglI    BglII
     BlpI Bme1580I     BmrI     BpmI    BpuEI     BsaI    BsaHI    BsaWI
    BsaXI    BseYI  BsiHKAI Bsp1286I    BspEI    BspMI    BsrBI    BsrFI
    BsrGI   BssHII    BstXI    BstYI   Bsu36I     BtgI     BtsI     EaeI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    HaeII    Hin4I
     KasI     MmeI     MscI     MslI   MspA1I     NaeI     NarI   NgoMIV
    PflMI   PspOMI     PstI     SacI    SacII     SapI     ScaI     SfcI
     SfoI     SmaI     SmlI     StyI    TaqII     TatI     TstI     XcmI
     XmaI     ZraI

 Enzymes that do not cut: 

     AccI   Acc65I     AclI    AflII   AflIII     AgeI     AhdI     AleI
     AlfI     AloI     ApoI     AscI     AseI    AsiSI     BaeI    BamHI
    BbvCI     BcgI    BciVI    BfrBI    BmgBI     BmtI     BplI   Bpu10I
    BsaAI    BsaBI    BseRI     BsgI    BsiEI    BsiWI     BsmI    BsmBI
    BspHI    BsrDI    BssSI   BstAPI    BstBI   BstEII  BstZ17I    BtgZI
     ClaI    CspCI     DraI   DraIII     DrdI     EagI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI   HincII  HindIII     HpaI     KpnI
     MfeI     MluI     NcoI     NdeI     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI    PspXI     PsrI     PvuI    PvuII    RsrII
     SalI    SanDI     SbfI    SexAI     SfiI     SgfI    SgrAI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI  Tth111I     XbaI
     XhoI     XmnI
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