Restriction map for pF1KB9833
 (Circular) (Six-base) MAPSORT of: pF1KB9833.seq  Check: 9695  from: 1  to: 4148



pF1KB9833  4148 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:32  ..

AccI GT'mk_AC

Cuts at:    279    1186     279 
   Size:        907    3241
 
Acc65I G'GTAC_C

Cuts at:   1164    1164 
   Size:       4148
 
AclI AA'CG_TT

Cuts at:   1142    3988    1142 
   Size:       2846    1302
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1973    2405    3152    3420    1973 
   Size:        432     747     268    2701

  Fragments arranged by size: 

                2701     747     432     268
 
AflII C'TTAA_G

Cuts at:    214     214 
   Size:       4148
 
AflIII A'CryG_T

Cuts at:    177    1500    2604    3581     177 
   Size:       1323    1104     977     744

  Fragments arranged by size: 

                1323    1104     977     744
 
AgeI A'CCGG_T

Cuts at:   2536    2536 
   Size:       4148
 
AlwNI CAG_nnn'CTG

Cuts at:   1453    3020    1453 
   Size:       1567    2581
 
ApaLI G'TGCA_C

Cuts at:   2918    2918 
   Size:       4148
 
ApoI r'AATT_y

Cuts at:    135     925    1083    1152    3501    3757     135 
   Size:        790     158      69    2349     256     526

  Fragments arranged by size: 

                2349     790     526     256     158      69
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4148
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4148
 
AvaI C'yCGr_G

Cuts at:   1168    1168 
   Size:       4148
 
BamHI G'GATC_C

Cuts at:   1173    1173 
   Size:       4148
 
BanI G'GyrC_C

Cuts at:   1164    1578    1826    1861    1164 
   Size:        414     248      35    3451

  Fragments arranged by size: 

                3451     414     248      35
 
BanII G_rGCy'C

Cuts at:   1162    2192    1162 
   Size:       1030    3118
 
BbeI G_GCGC'C

Cuts at:   1582    1830    1582 
   Size:        248    3900
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:     90     124      90 
   Size:         34    4114
 
BciVI GTATCCnnnnn_n'

Cuts at:    251    2046    2807    4148     251 
   Size:       1795     761    1341     251

  Fragments arranged by size: 

                1795    1341     761     251
 
BclI T'GATC_A

Cuts at:   1668    1668 
   Size:       4148
 
BglII A'GATC_T

Cuts at:   1663    3700    1663 
   Size:       2037    2111
 
BlpI GC'TnA_GC

Cuts at:   1260    1260 
   Size:       4148
 
Bme1580I G_kGCm'C

Cuts at:    236    1773    1866    2922     236 
   Size:       1537      93    1056    1462

  Fragments arranged by size: 

                1537    1462    1056      93
 
BmgBI CAC'GTC

Cuts at:    178     178 
   Size:       4148
 
BmrI ACTGGGnnnn_n'

Cuts at:    298    1360    1441    1776    3920     298 
   Size:       1062      81     335    2144     526

  Fragments arranged by size: 

                2144    1062     526     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2515    2515 
   Size:       4148
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    994     994 
   Size:       4148
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1324    2203    2695    2993    1324 
   Size:        879     492     298    2479

  Fragments arranged by size: 

                2479     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3852    3852 
   Size:       4148
 
BsaAI yAC'GTr

Cuts at:    447     715    1501     447 
   Size:        268     786    3094

  Fragments arranged by size: 

                3094     786     268
 
BsaBI GATnn'nnATC

Cuts at:      6    1662    3699       6 
   Size:       1656    2037     455

  Fragments arranged by size: 

                2037    1656     455
 
BsaHI Gr'CG_yC

Cuts at:   1579    1827    2529    4025    1579 
   Size:        248     702    1496    1702

  Fragments arranged by size: 

                1702    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:   1337    1858    2536    2810    2957    3368    1337 
   Size:        521     678     274     147     411    2117

  Fragments arranged by size: 

                2117     678     521     411     274     147
 
BseYI C'CCAG_C

Cuts at:    540     848    1573    2331    2908    3404     540 
   Size:        308     725     758     577     496    1284

  Fragments arranged by size: 

                1284     758     725     577     496     308
 
BsiEI CG_ry'CG

Cuts at:    104    1690    1736    2944     104 
   Size:       1586      46    1208    1308

  Fragments arranged by size: 

                1586    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:   1162    1940    2130    2922    1162 
   Size:        778     190     792    2388

  Fragments arranged by size: 

                2388     792     778     190
 
BsmBI CGTCTCn'nnnn_

Cuts at:    168     168 
   Size:       4148
 
Bsp1286I G_dGCh'C

Cuts at:    236    1162    1773    1866    1940    2130    2192    2922     236 
   Size:        926     611      93      74     190      62     730    1462

  Fragments arranged by size: 

                1462     926     730     611     190      93      74      62
            
 
BspEI T'CCGG_A

Cuts at:   3368    3368 
   Size:       4148
 
BspHI T'CATG_A

Cuts at:   3324    3324 
   Size:       4148
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1198    1412    1714    2095    1198 
   Size:        214     302     381    3251

  Fragments arranged by size: 

                3251     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2440    2494    3978    4143      50 
   Size:       2390      54    1484     165      55

  Fragments arranged by size: 

                2390    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2060    2060 
   Size:       4148
 
BsrFI r'CCGG_y

Cuts at:   2146    2327    2536    2146 
   Size:        181     209    3758

  Fragments arranged by size: 

                3758     209     181
 
BsrGI T'GTAC_A

Cuts at:    155     155 
   Size:       4148
 
BssSI C'ACGA_G

Cuts at:   2419    2777    2419 
   Size:        358    3790
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1525    1525 
   Size:       4148
 
BstBI TT'CG_AA

Cuts at:   2509    2509 
   Size:       4148
 
BstYI r'GATC_y

Cuts at:      1     372    1173    1640    1663    1998    2244    3256    3342 
   Size:        371     801     467      23     335     246    1012      86

Cuts at:   3342    3354    3700       1 
   Size:         12     346     449

  Fragments arranged by size: 

                1012     801     467     449     371     346     335     246
                  86      23      12
 
BstZ17I GTA'TAC

Cuts at:    280     280 
   Size:       4148
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    551    2098    2279    2442     551 
   Size:       1547     181     163    2257

  Fragments arranged by size: 

                2257    1547     181     163
 
BtsI GCAGTG_nn'

Cuts at:    641    1391     641 
   Size:        750    3398
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    312     347     312 
   Size:         35    4113
 
DraI TTT'AAA

Cuts at:   1147    1147 
   Size:       4148
 
DrdI GACnn_nn'nnGTC

Cuts at:   1854    2712    1854 
   Size:        858    3290
 
EaeI y'GGCC_r

Cuts at:    693    1733    1907    2298    2325    3670     693 
   Size:       1040     174     391      27    1345    1171

  Fragments arranged by size: 

                1345    1171    1040     391     174      27
 
EagI C'GGCC_G

Cuts at:   1733    1733 
   Size:       4148
 
EarI CTCTTCn'nnn_

Cuts at:    780    2171    2381     780 
   Size:       1391     210    2547

  Fragments arranged by size: 

                2547    1391     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2356    2666    2812    3958    2356 
   Size:        310     146    1146    2546

  Fragments arranged by size: 

                2546    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   1160    1160 
   Size:       4148
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    994    1973    2405    3152    3420     994 
   Size:        979     432     747     268    1722

  Fragments arranged by size: 

                1722     979     747     432     268
 
EcoO109I rG'GnC_Cy

Cuts at:   1287    1287 
   Size:       4148
 
EcoRI G'AATT_C

Cuts at:   1152    1152 
   Size:       4148
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2167    2167 
   Size:       4148
 
FspI TGC'GCA

Cuts at:   1929    1929 
   Size:       4148
 
HaeII r_GCGC'y

Cuts at:   1582    1830    2852    3831    1582 
   Size:        248    1022     979    1899

  Fragments arranged by size: 

                1899    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    529     561    1990    2020    2022    2052     529 
   Size:         32    1429      30       2      30    2625

  Fragments arranged by size: 

                2625    1429      32      30      30       2
 
HincII GTy'rAC

Cuts at:    183    1187     183 
   Size:       1004    3144
 
HindIII A'AGCT_T

Cuts at:    163     163 
   Size:       4148
 
KasI G'GCGC_C

Cuts at:   1578    1826    1578 
   Size:        248    3900
 
KpnI G_GTAC'C

Cuts at:   1168    1168 
   Size:       4148
 
MluI A'CGCG_T

Cuts at:   3581    3581 
   Size:       4148
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    458    2819    3003     458 
   Size:       2361     184    1603

  Fragments arranged by size: 

                2361    1603     184
 
MscI TGG'CCA

Cuts at:   1909    1909 
   Size:       4148
 
MslI CAynn'nnrTG

Cuts at:   2137    2264    2137 
   Size:        127    4021
 
MspA1I CmG'CkG

Cuts at:   1259    1573    1933    2946    3191    1259 
   Size:        314     360    1013     245    2216

  Fragments arranged by size: 

                2216    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   2329    2329 
   Size:       4148
 
NarI GG'CG_CC

Cuts at:   1579    1827    1579 
   Size:        248    3900
 
NgoMIV G'CCGG_C

Cuts at:   2327    2327 
   Size:       4148
 
NspI r_CATG'y

Cuts at:   1104    1201    1701    2608    1104 
   Size:         97     500     907    2644

  Fragments arranged by size: 

                2644     907     500      97
 
PciI A'CATG_T

Cuts at:   2604    2604 
   Size:       4148
 
PmeI GTTT'AAAC

Cuts at:   1147    1147 
   Size:       4148
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3287    3319    3287 
   Size:         32    4116
 
PstI C_TGCA'G

Cuts at:   1195    1880    1195 
   Size:        685    3463
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4148
 
PvuII CAG'CTG

Cuts at:   1573    1933    1573 
   Size:        360    3788
 
RsrII CG'GwC_CG

Cuts at:   2343    2343 
   Size:       4148
 
SacI G_AGCT'C

Cuts at:   1162    1162 
   Size:       4148
 
SalI G'TCGA_C

Cuts at:   1185    1185 
   Size:       4148
 
SapI GCTCTTCn'nnn_

Cuts at:   2171    2381    2171 
   Size:        210    3938
 
SbfI CC_TGCA'GG

Cuts at:   1195    1195 
   Size:       4148
 
ScaI AGT'ACT

Cuts at:   3665    3665 
   Size:       4148
 
SfcI C'TryA_G

Cuts at:     33    1191    1876    2869    3060      33 
   Size:       1158     685     993     191    1121

  Fragments arranged by size: 

                1158    1121     993     685     191
 
SfoI GGC'GCC

Cuts at:   1580    1828    1580 
   Size:        248    3900
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4148
 
SmaI CCC'GGG

Cuts at:   1170    1170 
   Size:       4148
 
SmlI C'TyrA_G

Cuts at:    214    1303    2218    2710    2972     214 
   Size:       1089     915     492     262    1390

  Fragments arranged by size: 

                1390    1089     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4148
 
SphI G_CATG'C

Cuts at:   1201    1701    1201 
   Size:        500    3648
 
StuI AGG'CCT

Cuts at:   1052    1052 
   Size:       4148
 
StyI C'CwwG_G

Cuts at:    920    1282    1636     920 
   Size:        362     354    3432

  Fragments arranged by size: 

                3432     362     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1731    1864    2323    2532    1731 
   Size:        133     459     209    3347

  Fragments arranged by size: 

                3347     459     209     133
 
TatI w'GTAC_w

Cuts at:    155    1013    3663     155 
   Size:        858    2650     640

  Fragments arranged by size: 

                2650     858     640
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3665    3697    3665 
   Size:         32    4116
 
Tth111I GACn'n_nGTC

Cuts at:   1945    1945 
   Size:       4148
 
XbaI T'CTAG_A

Cuts at:   1179    1179 
   Size:       4148
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1643    1643 
   Size:       4148
 
XmaI C'CCGG_G

Cuts at:   1168    1168 
   Size:       4148
 
XmnI GAAnn'nnTTC

Cuts at:    209     209 
   Size:       4148
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI    AlwNI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI     BcgI    BciVI     BclI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BseYI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI    BstBI
    BstYI  BstZ17I    BtgZI     BtsI    CspCI     DraI     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI
     FspI    HaeII    Hin4I   HincII  HindIII     KasI     KpnI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI   NgoMIV     NspI
     PciI     PmeI     PpiI     PstI     PvuI    PvuII    RsrII     SacI
     SalI     SapI     SbfI     ScaI     SfcI     SfoI     SgfI     SmaI
     SmlI     SpeI     SphI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI     AhdI     AleI     AlfI     AloI     ApaI
     AscI    AvrII     BaeI     BbsI    BbvCI    BfrBI     BglI     BmtI
   Bpu10I    BsaXI    BseRI     BsgI    BsiWI     BsmI   BssHII   BstEII
    BstXI   Bsu36I     BtgI     ClaI   DraIII    EcoNI    EcoRV     FseI
    FspAI     HpaI     MfeI     NcoI     NdeI     NheI     NotI     NruI
     NsiI     PacI     PasI    PflMI     PfoI     PmlI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI    SacII    SanDI    SexAI     SfiI
    SgrAI    SnaBI     SrfI     SspI     SwaI     XhoI     ZraI
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