Restriction map for pF1KB9831
##################################
# NO CUT ENZYMES (  130 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AflII       AflIII      AgeI        AhdI        AlwNI       
AocI        Aor51HI     ApaI        AscI        Asp700I     
Asp718I     AspEI       AspI        AvrII       BamHI       
BanI        BanII       BbeI        BbvCI       BcgI        
BfaI        BfrI        BglI        BlnI        BpmI        
BsaI        BsaWI       BseAI       BsiEI       BsiWI       
BsmAI       BsmBI       BsmFI       BsmI        Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrGI       BssHII      BssSI       Bst1107I    
BstBI       BstUI       BstZ17I     Bsu36I      ClaI        
CpoI        DraI        DraII       DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO109I    EcoRI       EcoRV       EcoT22I     
FseI        HincII      HindII      HindIII     HpaI        
KasI        KpnI        KspI        MaeI        MfeI        
MluI        MroI        MunI        MvnI        NaeI        
NarI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmeI        PpuMI       PshAI       
Psp1406I    PstI        PvuI        RcaI        RsrII       
SacI        SacII       SalI        SbfI        ScaI        
SfiI        SfoI        SfuI        SgrAI       SmlI        
SnaBI       SpeI        Sse8387I    SspBI       SspI        
StuI        SwaI        TaqI        TfiI        Tth111I     
TthHB8I     XbaI        XcmI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

AccI          223  GTCTAC               GT^[AC][GT]AC
AcyI          325  GGCGTC               G[GA]^CG[CT]C
Alw44I        702  GTGCAC               G^TGCAC
AlwI         1002  GATCC                GGATC(4/5)
ApaLI         702  GTGCAC               G^TGCAC
AseI          811  ATTAAT               AT^TAAT
AsnI          811  ATTAAT               AT^TAAT
AvaII         764  GGACC                G^G[AT]CC
AviII         932  TGCGCA               TGC^GCA
BalI          428  TGGCCA               TGG^CCA
BbrPI         745  CACGTG               CAC^GTG
BbsI          592  GTCTTC               GAAGAC(2/6)
BclI          200  TGATCA               T^GATCA
BglII         158  AGATCT               A^GATCT
BlpI          772  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      772  GCTGAGC              GC^T[ACGT]AGC
BpuAI         592  GTCTTC               GAAGAC(2/6)
BsaHI         325  GGCGTC               G[GA]^CG[CT]C
BseRI          15  CTCCTC               GAGGAG(10/8)
BsrDI          90  CATTGC               GCAATG(2/0)
BsrFI         648  GCCGGT               [GA]^CCGG[CT]
BstAPI        645  GCAGCCGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        828  GGTCACC              G^GT[ACGT]ACC
BstPI         828  GGTCACC              G^GT[ACGT]ACC
BstXI         999  CCAGATCCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII         772  GCTGAGC              GC^T[ACGT]AGC
Cfr10I        648  GCCGGT               [GA]^CCGG[CT]
EaeI          428  TGGCCA               [CT]^GGCC[GA]
EcoO65I       828  GGTCACC              G^GT[ACGT]ACC
FbaI          200  TGATCA               T^GATCA
FspI          932  TGCGCA               TGC^GCA
HgaI          326  GCGTC                GACGC(5/10)
Hin1I         325  GGCGTC               G[GA]^CG[CT]C
MluNI         428  TGGCCA               TGG^CCA
MscI          428  TGGCCA               TGG^CCA
MseI          812  TTAA                 T^TAA
MspA1I        754  CAGCTG               C[AC]G^C[GT]G
NcoI          179  CCATGG               C^CATGG
PflMI         741  CCACCACGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI         745  CACGTG               CAC^GTG
PmlI          745  CACGTG               CAC^GTG
PshBI         811  ATTAAT               AT^TAAT
PvuII         754  CAGCTG               CAG^CTG
SapI          192  GCTCTTC              GCTCTTC(1/4)
SexAI         617  ACCTGGT              A^CC[AT]GGT
SfcI          924  CTACAG               C^T[GA][CT]AG
SmaI          953  CCCGGG               CCC^GGG
SphI          929  GCATGC               GCATG^C
Tru9I         812  TTAA                 T^TAA
Van91I        741  CCACCACGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI         953  CCCGGG               C^CCGGG
XmaI          953  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AfaI          129  GTAC                 GT^AC
              632  GTAC                
AspHI          79  GTGCTC               G[AT]GC[AT]^C
              702  GTGCAC              
AvaI           82  CTCGGG               C^[CT]CG[GA]G
              953  CCCGGG              
BciVI         248  GGATAC               GTATCC(6/5)
              966  GGATAC              
BsaAI         103  TACGTG               [CT]AC^GT[GA]
              745  CACGTG              
BsaBI         153  GATAAAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              817  GATGCACATC          
BsgI          652  GTGCAG               GTGCAG(16/14)
              751  GTGCAG              
BsiHKAI        79  GTGCTC               G[AT]GC[AT]^C
              702  GTGCAC              
BsoBI          82  CTCGGG               C^[CT]CG[GA]G
              953  CCCGGG              
BsrI          245  ACTGG                ACTGG(1/-1)
              547  CCAGT               
BstYI         158  AGATCT               [GA]^GATC[CT]
             1001  AGATCC              
DraIII        742  CACCACGTG            CAC[ACGT][ACGT][ACGT]^GTG
              745  CACGTGGTG           
DsaI          179  CCATGG               C^C[GA][CT]GG
              962  CCACGG              
EcoT14I       179  CCATGG               C^C[AT][AT]GG
              413  CCTTGG              
FauI          122  CCCGC                CCCGC(4/6)
              389  CCCGC               
HaeII         680  AGCGCC               [GA]GCGC^[CT]
              687  GGCGCT              
MamI          153  GATAAAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              817  GATGCACATC          
MflI          158  AGATCT               [GA]^GATC[CT]
             1001  AGATCC              
NlaIV           3  GGAGCC               GG[ACGT]^[ACGT]CC
              764  GGACCC              
NspI          188  ACATGC               [GA]CATG^[CT]
              929  GCATGC              
RsaI          129  GTAC                 GT^AC
              632  GTAC                
StyI          179  CCATGG               C^C[AT][AT]GG
              413  CCTTGG              
XhoII         158  AGATCT               [GA]^GATC[CT]
             1001  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   53 enzymes) #
#########################################

AciI        AcsI        AluI        ApoI        BbvI        
BcnI        BmyI        BsaJI       BsiYI       BslI        
Bsp1286I    BssKI       BstF5I      BstNI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       EarI        
EcoRII      Fnu4HI      FokI        HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeII       MaeIII      MboI        
MboII       MnlI        MslI        MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      PleI        
Sau3AI      Sau96I      ScrFI       SfaNI       TseI        
Tsp45I      Tsp509I     TspRI       
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