Restriction map for pF1KB9812
 (Linear) (Six-base) MAPSORT of: KIBB9812.seq  Check: 7531  from: 1  to: 771



KIBB9812.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:12  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     668     771 
   Size:        668     103
 
AflIII A'CryG_T

Cuts at:      0     728     771 
   Size:        728      43
 
AhdI GACnn_n'nnGTC

Cuts at:      0     514     771 
   Size:        514     257
 
ApaI G_GGCC'C

Cuts at:      0     230     771 
   Size:        230     541
 
AvaI C'yCGr_G

Cuts at:      0     206     771 
   Size:        206     565
 
BanII G_rGCy'C

Cuts at:      0     230     341     771 
   Size:        230     111     430

  Fragments arranged by size: 

                 430     230     111
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      89     771 
   Size:         89     682
 
BbvCI CC'TCA_GC

Cuts at:      0     197     771 
   Size:        197     574
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     710     771 
   Size:        710      61
 
BlpI GC'TnA_GC

Cuts at:      0     316     771 
   Size:        316     455
 
Bme1580I G_kGCm'C

Cuts at:      0     230     771 
   Size:        230     541
 
BmgBI CAC'GTC

Cuts at:      0     731     771 
   Size:        731      40
 
Bpu10I CC'TnA_GC

Cuts at:      0     197     771 
   Size:        197     574
 
BsaAI yAC'GTr

Cuts at:      0     369     771 
   Size:        369     402
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     127     157     771 
   Size:        127      30     614

  Fragments arranged by size: 

                 614     127      30
 
BseYI C'CCAG_C

Cuts at:      0     525     771 
   Size:        525     246
 
BsiHKAI G_wGCw'C

Cuts at:      0     184     678     771 
   Size:        184     494      93

  Fragments arranged by size: 

                 494     184      93
 
BsmI GAATG_Cn'

Cuts at:      0     241     771 
   Size:        241     530
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     282     771 
   Size:        282     489
 
Bsp1286I G_dGCh'C

Cuts at:      0     184     230     341     678     771 
   Size:        184      46     111     337      93

  Fragments arranged by size: 

                 337     184     111      93      46
 
BsrBI CCG'CTC

Cuts at:      0     219     771 
   Size:        219     552
 
BsrDI GCAATG_nn'

Cuts at:      0     266     771 
   Size:        266     505
 
BsrFI r'CCGG_y

Cuts at:      0     597     771 
   Size:        597     174
 
BssSI C'ACGA_G

Cuts at:      0     700     771 
   Size:        700      71
 
BstEII G'GTnAC_C

Cuts at:      0     474     771 
   Size:        474     297
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     153     771 
   Size:        153     618
 
BtgI C'CryG_G

Cuts at:      0      39     304     380     771 
   Size:         39     265      76     391

  Fragments arranged by size: 

                 391     265      76      39
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     594     771 
   Size:        594     177
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     483     771 
   Size:        483     288
 
EaeI y'GGCC_r

Cuts at:      0     491     771 
   Size:        491     280
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     420     771 
   Size:        420     351
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     668     771 
   Size:        668     103
 
EcoO109I rG'GnC_Cy

Cuts at:      0     226     309     771 
   Size:        226      83     462

  Fragments arranged by size: 

                 462     226      83
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     472     500     504     532     771 
   Size:        472      28       4      28     239

  Fragments arranged by size: 

                 472     239      28      28       4
 
MspA1I CmG'CkG

Cuts at:      0     306     771 
   Size:        306     465
 
PpuMI rG'GwC_Cy

Cuts at:      0     309     771 
   Size:        309     462
 
PspOMI G'GGCC_C

Cuts at:      0     226     771 
   Size:        226     545
 
SacII CC_GC'GG

Cuts at:      0     307     771 
   Size:        307     464
 
ScaI AGT'ACT

Cuts at:      0     445     771 
   Size:        445     326
 
SexAI A'CCwGG_T

Cuts at:      0     512     771 
   Size:        512     259
 
SspI AAT'ATT

Cuts at:      0     565     771 
   Size:        565     206
 
StyI C'CwwG_G

Cuts at:      0      48     332     771 
   Size:         48     284     439

  Fragments arranged by size: 

                 439     284      48
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     462     493     771 
   Size:        462      31     278

  Fragments arranged by size: 

                 462     278      31
 
TatI w'GTAC_w

Cuts at:      0     443     771 
   Size:        443     328
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     293     325     771 
   Size:        293      32     446

  Fragments arranged by size: 

                 446     293      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     153     771 
   Size:        153     618
 

 Enzymes that do cut:

     AcuI   AflIII     AhdI     ApaI     AvaI    BanII     BbsI    BbvCI
    BciVI     BlpI Bme1580I    BmgBI   Bpu10I    BsaAI    BsaXI    BseYI
  BsiHKAI     BsmI    BsmBI Bsp1286I    BsrBI    BsrDI    BsrFI    BssSI
   BstEII    BstXI     BtgI    BtgZI     DrdI     EaeI     EciI  Eco57MI
 EcoO109I    Hin4I   MspA1I    PpuMI   PspOMI    SacII     ScaI    SexAI
     SspI     StyI    TaqII     TatI     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AleI     AlfI     AloI    AlwNI    ApaLI     ApoI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI     BcgI     BclI
    BfrBI     BglI    BglII     BmrI     BmtI     BplI     BpmI    BpuEI
     BsaI    BsaBI    BsaHI    BsaWI    BseRI     BsgI    BsiEI    BsiWI
    BspEI    BspHI    BspMI    BsrGI   BssHII   BstAPI    BstBI    BstYI
  BstZ17I   Bsu36I     BtsI     ClaI    CspCI     DraI   DraIII     EagI
     EarI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI     MslI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI     SalI
    SanDI     SapI     SbfI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     StuI     SwaI
  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI
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