Restriction map for pF1KB9811
##################################
# NO CUT ENZYMES (  145 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AflII       AflIII      AhdI        
Alw44I      AocI        Aor51HI     ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AvaII       AviII       AvrII       
BamHI       BbrPI       BcgI        BciVI       BclI        
BfaI        BfrI        BglII       BlnI        BlpI        
Bpu1102I    BsaAI       BsaBI       BsaI        BseAI       
BsiWI       BsmBI       BsmI        Bsp1407I    BspDI       
BspEI       BspLU11I    BsrBI       BsrDI       BsrGI       
BssHII      BssSI       Bst1107I    BstBI       BstEII      
BstPI       BstUI       BstYI       BstZ17I     Bsu36I      
CelII       ClaI        CpoI        DraI        DraIII      
DrdI        Eam1105I    Eco47III    Eco81I      EcoO65I     
EcoRI       EcoRV       EcoT22I     FauI        FbaI        
FseI        FspI        HincII      HindII      HindIII     
HpaI        KpnI        KspI        MaeI        MamI        
MfeI        MflI        MluI        MroI        MseI        
MslI        MunI        MvnI        NaeI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
RsrII       SacII       SalI        SbfI        ScaI        
SexAI       SfcI        SfuI        SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        TfiI        Tru9I       
Tsp509I     Tth111I     Van91I      XbaI        XcmI        
XhoI        XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AfaI          249  GTAC                 GT^AC
AgeI          406  ACCGGT               A^CCGGT
AlwI           18  GATCC                GGATC(4/5)
AlwNI         661  CAGCTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI          232  GGGCCC               GGGCC^C
BanI           82  GGCGCC               G^G[CT][GA]CC
BbeI           82  GGCGCC               GGCGC^C
BbvCI         621  CCTCAGC              CCTCAGC(-5/-2)
BsaWI         406  ACCGGT               [AT]^CCGG[AT]
BsgI          613  CTGCAC               GTGCAG(16/14)
BsiEI         692  CGGCCG               CG[GA][CT]^CG
BsmFI         335  GGGAC                GGGAC(10/14)
BspHI          74  TCATGA               T^CATGA
BspMI         125  ACCTGC               ACCTGC(4/8)
BsrFI         406  ACCGGT               [GA]^CCGG[CT]
BstAPI        660  GCAGCTGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I        501  CATCC                GGATG(2/0)
Cfr10I        406  ACCGGT               [GA]^CCGG[CT]
DraII         231  GGGGCCC              [GA]G^G[ACGT]CC[CT]
EagI          692  CGGCCG               C^GGCCG
EclXI         692  CGGCCG               C^GGCCG
Eco52I        692  CGGCCG               C^GGCCG
EcoO109I      231  GGGGCCC              [GA]G^G[ACGT]CC[CT]
FokI          501  CATCC                GGATG(9/13)
HaeII          82  GGCGCC               [GA]GCGC^[CT]
HgaI          337  GACGC                GACGC(5/10)
KasI           82  GGCGCC               G^GCGCC
MaeII         149  ACGT                 A^CGT
MspA1I        661  CAGCTG               C[AC]G^C[GT]G
NarI           82  GGCGCC               GG^CGCC
PinAI         406  ACCGGT               A^CCGGT
PvuII         661  CAGCTG               CAG^CTG
RcaI           74  TCATGA               T^CATGA
RsaI          249  GTAC                 GT^AC
SapI          115  GAAGAGC              GCTCTTC(1/4)
SfiI          393  GGCCGAGGTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI           82  GGCGCC               GGC^GCC
SgrAI         405  CACCGGTG             C[GA]^CCGG[CT]G
TaqI          593  TCGA                 T^CGA
TspRI         683  CACTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I       593  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   32 enzymes) #
###################################

AcyI           82  GGCGCC               G[GA]^CG[CT]C
              337  GACGCC              
AspHI         103  GAGCTC               G[AT]GC[AT]^C
              271  GAGCTC              
AvaI          235  CCCGAG               C^[CT]CG[GA]G
              559  CCCGAG              
BalI          401  TGGCCA               TGG^CCA
              578  TGGCCA              
BbsI          352  GTCTTC               GAAGAC(2/6)
              532  GTCTTC              
BglI           28  GCCAAGCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              394  GCCGAGGTGGC         
BpmI          274  CTCCAG               CTGGAG(16/14)
              174  CTGGAG              
BpuAI         352  GTCTTC               GAAGAC(2/6)
              532  GTCTTC              
BsaHI          82  GGCGCC               G[GA]^CG[CT]C
              337  GACGCC              
BsiHKAI       103  GAGCTC               G[AT]GC[AT]^C
              271  GAGCTC              
BsmAI         265  GAGAC                GTCTC(1/5)
              671  GAGAC               
BsoBI         235  CCCGAG               C^[CT]CG[GA]G
              559  CCCGAG              
BsrI           11  CCAGT                ACTGG(1/-1)
              188  CCAGT               
BstXI         500  CCATCCGCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              569  CCATCTCTCTGG        
CfoI           83  GCGC                 GCG^C
              423  GCGC                
EarI          115  GAAGAG               CTCTTC(1/4)
              709  GAAGAG              
EcoT14I       461  CCATGG               C^C[AT][AT]GG
              602  CCATGG              
HhaI           83  GCGC                 GCG^C
              423  GCGC                
Hin1I          82  GGCGCC               G[GA]^CG[CT]C
              337  GACGCC              
HinP1I         83  GCGC                 G^CGC
              423  GCGC                
HinfI         433  GACTC                G^A[ACGT]TC
              631  GACTC               
HphI          373  GGTGA                GGTGA(8/7)
              409  GGTGA               
Ksp632I       115  GAAGAG               CTCTTC(1/4)
              709  GAAGAG              
MaeIII        374  GTGAC                ^GT[ACGT]AC
              410  GTGAC               
MluNI         401  TGGCCA               TGG^CCA
              578  TGGCCA              
MscI          401  TGGCCA               TGG^CCA
              578  TGGCCA              
NcoI          461  CCATGG               C^CATGG
              602  CCATGG              
PleI          433  GACTC                GAGTC(4/5)
              631  GACTC               
SacI          103  GAGCTC               GAGCT^C
              271  GAGCTC              
SfaNI         483  GATGC                GCATC(5/9)
              425  GCATC               
StyI          461  CCATGG               C^C[AT][AT]GG
              602  CCATGG              
Tsp45I        374  GTGAC                ^GT[GC]AC
              410  GTGAC               


#########################################
# 3 OR MORE CUT ENZYMES (   40 enzymes) #
#########################################

AciI        AluI        BanII       BbvI        BcnI        
BmyI        BsaJI       BseRI       BsiYI       BslI        
Bsp1286I    BssKI       BstNI       Cac8I       Cfr13I      
DdeI        DpnII       DsaI        EaeI        EcoNI       
EcoRII      Fnu4HI      HaeIII      HapII       HpaII       
ItaI        MboI        MboII       MnlI        MspI        
MvaI        MwoI        NciI        NdeII       NlaIII      
NlaIV       Sau3AI      Sau96I      ScrFI       TseI        
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