Restriction map for pF1KB9811
 (Linear) (Six-base) MAPSORT of: KIBB9811.seq  Check: 5110  from: 1  to: 744



KIBB9811.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:12  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     382     744 
   Size:        382     362
 
AgeI A'CCGG_T

Cuts at:      0     406     744 
   Size:        406     338
 
AlwNI CAG_nnn'CTG

Cuts at:      0     666     744 
   Size:        666      78
 
ApaI G_GGCC'C

Cuts at:      0     236     744 
   Size:        236     508
 
AvaI C'yCGr_G

Cuts at:      0     235     559     744 
   Size:        235     324     185

  Fragments arranged by size: 

                 324     235     185
 
BanI G'GyrC_C

Cuts at:      0      82     744 
   Size:         82     662
 
BanII G_rGCy'C

Cuts at:      0     107     220     236     275     737     744 
   Size:        107     113      16      39     462       7

  Fragments arranged by size: 

                 462     113     107      39      16       7
 
BbeI G_GCGC'C

Cuts at:      0      86     744 
   Size:         86     658
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     345     525     744 
   Size:        345     180     219

  Fragments arranged by size: 

                 345     219     180
 
BbvCI CC'TCA_GC

Cuts at:      0     622     744 
   Size:        622     122
 
BglI GCCn_nnn'nGGC

Cuts at:      0      34     400     744 
   Size:         34     366     344

  Fragments arranged by size: 

                 366     344      34
 
Bme1580I G_kGCm'C

Cuts at:      0     236     744 
   Size:        236     508
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     123     234     588     744 
   Size:        123     111     354     156

  Fragments arranged by size: 

                 354     156     123     111
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     195     259     744 
   Size:        195      64     485

  Fragments arranged by size: 

                 485     195      64
 
Bpu10I CC'TnA_GC

Cuts at:      0     622     744 
   Size:        622     122
 
BsaHI Gr'CG_yC

Cuts at:      0      83     338     744 
   Size:         83     255     406

  Fragments arranged by size: 

                 406     255      83
 
BsaWI w'CCGG_w

Cuts at:      0     406     744 
   Size:        406     338
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     115     226     238     577     744 
   Size:        115     111      12     339     167

  Fragments arranged by size: 

                 339     167     115     111      12
 
BseYI C'CCAG_C

Cuts at:      0     297     744 
   Size:        297     447
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     598     744 
   Size:        598     146
 
BsiEI CG_ry'CG

Cuts at:      0     695     744 
   Size:        695      49
 
BsiHKAI G_wGCw'C

Cuts at:      0     107     275     744 
   Size:        107     168     469

  Fragments arranged by size: 

                 469     168     107
 
Bsp1286I G_dGCh'C

Cuts at:      0     107     220     236     275     737     744 
   Size:        107     113      16      39     462       7

  Fragments arranged by size: 

                 462     113     107      39      16       7
 
BspHI T'CATG_A

Cuts at:      0      74     744 
   Size:         74     670
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     134     744 
   Size:        134     610
 
BsrFI r'CCGG_y

Cuts at:      0     406     744 
   Size:        406     338
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     666     744 
   Size:        666      78
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     507     576     744 
   Size:        507      69     168

  Fragments arranged by size: 

                 507     168      69
 
BtgI C'CryG_G

Cuts at:      0      86     461     602     744 
   Size:         86     375     141     142

  Fragments arranged by size: 

                 375     142     141      86
 
EaeI y'GGCC_r

Cuts at:      0     159     392     401     578     692     744 
   Size:        159     233       9     177     114      52

  Fragments arranged by size: 

                 233     177     159     114      52       9
 
EagI C'GGCC_G

Cuts at:      0     692     744 
   Size:        692      52
 
EarI CTCTTCn'nnn_

Cuts at:      0     110     704     744 
   Size:        110     594      40

  Fragments arranged by size: 

                 594     110      40
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     493     744 
   Size:        493     251
 
EcoICRI GAG'CTC

Cuts at:      0     105     273     744 
   Size:        105     168     471

  Fragments arranged by size: 

                 471     168     105
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     195     259     382     744 
   Size:        195      64     123     362

  Fragments arranged by size: 

                 362     195     123      64
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     322     394     625     744 
   Size:        322      72     231     119

  Fragments arranged by size: 

                 322     231     119      72
 
EcoO109I rG'GnC_Cy

Cuts at:      0     232     744 
   Size:        232     512
 
HaeII r_GCGC'y

Cuts at:      0      86     744 
   Size:         86     658
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      29     744 
   Size:         29     715
 
KasI G'GCGC_C

Cuts at:      0      82     744 
   Size:         82     662
 
MscI TGG'CCA

Cuts at:      0     403     580     744 
   Size:        403     177     164

  Fragments arranged by size: 

                 403     177     164
 
MspA1I CmG'CkG

Cuts at:      0     663     744 
   Size:        663      81
 
NarI GG'CG_CC

Cuts at:      0      83     744 
   Size:         83     661
 
NcoI C'CATG_G

Cuts at:      0     461     602     744 
   Size:        461     141     142

  Fragments arranged by size: 

                 461     142     141
 
PspOMI G'GGCC_C

Cuts at:      0     232     744 
   Size:        232     512
 
PvuII CAG'CTG

Cuts at:      0     663     744 
   Size:        663      81
 
SacI G_AGCT'C

Cuts at:      0     107     275     744 
   Size:        107     168     469

  Fragments arranged by size: 

                 469     168     107
 
SapI GCTCTTCn'nnn_

Cuts at:      0     110     744 
   Size:        110     634
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     400     744 
   Size:        400     344
 
SfoI GGC'GCC

Cuts at:      0      84     744 
   Size:         84     660
 
SgrAI Cr'CCGG_yG

Cuts at:      0     406     744 
   Size:        406     338
 
StyI C'CwwG_G

Cuts at:      0     461     602     744 
   Size:        461     141     142

  Fragments arranged by size: 

                 461     142     141
 

 Enzymes that do cut:

     AcuI     AgeI    AlwNI     ApaI     AvaI     BanI    BanII     BbeI
     BbsI    BbvCI     BglI Bme1580I     BplI     BpmI   Bpu10I    BsaHI
    BsaWI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspHI
    BspMI    BsrFI   BstAPI    BstXI     BtgI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    HaeII    Hin4I     KasI
     MscI   MspA1I     NarI     NcoI   PspOMI    PvuII     SacI     SapI
     SfiI     SfoI    SgrAI     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AhdI     AleI     AlfI     AloI    ApaLI     ApoI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BcgI    BciVI     BclI    BfrBI
    BglII     BlpI    BmgBI     BmrI     BmtI    BpuEI     BsaI    BsaAI
    BsaBI    BsaXI    BsiWI     BsmI    BsmBI    BspEI    BsrBI    BsrDI
    BsrGI   BssHII    BssSI    BstBI   BstEII    BstYI  BstZ17I   Bsu36I
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI   HincII  HindIII     HpaI
     KpnI     MfeI     MluI     MmeI     MslI     NaeI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
    PspXI     PsrI     PstI     PvuI    RsrII    SacII     SalI    SanDI
     SbfI     ScaI    SexAI     SfcI     SgfI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI
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