Restriction map for pF1KB9810
##################################
# NO CUT ENZYMES (  154 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AflII       AgeI        AhdI        
Alw44I      AlwI        AlwNI       AocI        Aor51HI     
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AvaI        AviII       AvrII       BalI        BamHI       
BbsI        BbvCI       BcgI        BciVI       BclI        
BcnI        BfrI        BglI        BglII       BlnI        
BpuAI       BsaBI       BsaWI       BseAI       BsgI        
BsiEI       BsiHKAI     BsmBI       BsmI        BsoBI       
BspDI       BspEI       BspHI       BsrBI       BsrDI       
BsrFI       BssHII      BssSI       Bst1107I    BstAPI      
BstEII      BstPI       BstUI       BstXI       BstYI       
BstZ17I     Bsu36I      Cfr10I      ClaI        CpoI        
DpnII       DraI        DraIII      DrdI        EaeI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        HincII      HindII      
HindIII     HpaI        KpnI        KspI        MamI        
MboI        MfeI        MflI        MluI        MluNI       
MroI        MscI        MspA1I      MunI        MvnI        
NaeI        NciI        NdeI        NdeII       NgoMIV      
NheI        NotI        NruI        NsiI        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmeI        
PshAI       PshBI       Psp1406I    PvuI        PvuII       
RcaI        RsrII       SacI        SacII       SalI        
Sau3AI      SbfI        ScaI        SexAI       SfiI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspI        StuI        SwaI        
Tth111I     Van91I      XbaI        XcmI        XhoI        
XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AcyI          380  GGCGCC               G[GA]^CG[CT]C
BanI          380  GGCGCC               G^G[CT][GA]CC
BbeI          380  GGCGCC               GGCGC^C
BbrPI         193  CACGTG               CAC^GTG
BfaI          349  CTAG                 C^TAG
BlpI          411  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      411  GCTGAGC              GC^T[ACGT]AGC
BsaHI         380  GGCGCC               G[GA]^CG[CT]C
BsaI          547  GAGACC               GGTCTC(1/5)
BseRI         311  CTCCTC               GAGGAG(10/8)
BsiWI         638  CGTACG               C^GTACG
BsmAI         547  GAGAC                GTCTC(1/5)
BspMI         151  GCAGGT               ACCTGC(4/8)
BstBI         187  TTCGAA               TT^CGAA
CelII         411  GCTGAGC              GC^T[ACGT]AGC
CfoI          381  GCGC                 GCG^C
EcoNI         498  CCTTGAAAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII         380  GGCGCC               [GA]GCGC^[CT]
HapII         378  CCGG                 C^CGG
HgaI          256  GACGC                GACGC(5/10)
HhaI          381  GCGC                 GCG^C
Hin1I         380  GGCGCC               G[GA]^CG[CT]C
HinP1I        381  GCGC                 G^CGC
HpaII         378  CCGG                 C^CGG
HphI          279  GGTGA                GGTGA(8/7)
KasI          380  GGCGCC               G^GCGCC
MaeI          349  CTAG                 C^TAG
MseI          728  TTAA                 T^TAA
MslI          363  CATGTCCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI          378  CCGG                 C^CGG
NarI          380  GGCGCC               GG^CGCC
NspV          187  TTCGAA               TT^CGAA
PleI          123  GACTC                GAGTC(4/5)
PmaCI         193  CACGTG               CAC^GTG
PmlI          193  CACGTG               CAC^GTG
PpuMI         506  AGGTCCC              [GA]G^G[AT]CC[CT]
SapI          202  GAAGAGC              GCTCTTC(1/4)
SfoI          380  GGCGCC               GGC^GCC
SfuI          187  TTCGAA               TT^CGAA
TaqI          188  TCGA                 T^CGA
TfiI          686  GATTC                G^A[AT]TC
Tru9I         728  TTAA                 T^TAA
TthHB8I       188  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

AluI          206  AGCT                 AG^CT
              410  AGCT                
ApaI          142  GGGCCC               GGGCC^C
              647  GGGCCC              
BanII         142  GGGCCC               G[GA]GC[CT]^C
              647  GGGCCC              
BmyI          142  GGGCCC               G[AGT]GC[ACT]^C
              647  GGGCCC              
BpmI          127  CTGGAG               CTGGAG(16/14)
              328  CTGGAG              
BsaAI         193  CACGTG               [CT]AC^GT[GA]
              640  TACGTG              
Bsp1286I      142  GGGCCC               G[AGT]GC[ACT]^C
              647  GGGCCC              
Bsp1407I      266  TGTACA               T^GTACA
              715  TGTACA              
BspLU11I      362  ACATGT               A^CATGT
              473  ACATGT              
BsrGI         266  TGTACA               T^GTACA
              715  TGTACA              
BsrI          618  CCAGT                ACTGG(1/-1)
              633  CCAGT               
BssKI         103  CCAGG                ^CC[ACGT]GG
              275  CCAGG               
BstNI         103  CCAGG                CC^[AT]GG
              275  CCAGG               
DdeI          286  CTCAG                C^T[ACGT]AG
              412  CTGAG               
DraII         506  AGGTCCC              [GA]G^G[ACGT]CC[CT]
              647  GGGCCCT             
EarI          314  CTCTTC               CTCTTC(1/4)
              202  GAAGAG              
EcoO109I      506  AGGTCCC              [GA]G^G[ACGT]CC[CT]
              647  GGGCCCT             
EcoRII        103  CCAGG                ^CC[AT]GG
              275  CCAGG               
HinfI         123  GACTC                G^A[ACGT]TC
              686  GATTC               
Ksp632I       314  CTCTTC               CTCTTC(1/4)
              202  GAAGAG              
MvaI          103  CCAGG                CC^[AT]GG
              275  CCAGG               
MwoI          300  GCCTTTGGAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              584  GCTGCAGGGGC         
NspI          362  ACATGT               [GA]CATG^[CT]
              473  ACATGT              
PstI          464  CTGCAG               CTGCA^G
              585  CTGCAG              
ScrFI         103  CCAGG                CC^[ACGT]GG
              275  CCAGG               
SfaNI         282  GATGC                GCATC(5/9)
              352  GATGC               
SfcI          464  CTGCAG               C^T[GA][CT]AG
              585  CTGCAG              
SspBI         266  TGTACA               T^GTACA
              715  TGTACA              
Tsp45I        422  GTGAC                ^GT[GC]AC
              490  GTCAC               
Tsp509I       343  AATT                 ^AATT
              675  AATT                
TspRI         467  CAGTG                CA[GC]TG[ACGT][ACGT]^
              619  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   30 enzymes) #
#########################################

AciI        AfaI        AflIII      AvaII       BbvI        
BsaJI       BsiYI       BslI        BsmFI       BstF5I      
Cac8I       Cfr13I      DsaI        EcoT14I     FauI        
Fnu4HI      FokI        HaeIII      ItaI        MaeII       
MaeIII      MboII       MnlI        NcoI        NlaIII      
NlaIV       RsaI        Sau96I      StyI        TseI        
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com