Restriction map for pF1KB9810
 (Linear) (Six-base) MAPSORT of: KIBB9810.seq  Check: 9901  from: 1  to: 741



KIBB9810.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:12  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     550     741 
   Size:        550     191
 
AflIII A'CryG_T

Cuts at:      0     192     269     362     473     741 
   Size:        192      77      93     111     268

  Fragments arranged by size: 

                 268     192     111      93      77
 
ApaI G_GGCC'C

Cuts at:      0     146     651     741 
   Size:        146     505      90

  Fragments arranged by size: 

                 505     146      90
 
BanI G'GyrC_C

Cuts at:      0     380     741 
   Size:        380     361
 
BanII G_rGCy'C

Cuts at:      0     146     651     741 
   Size:        146     505      90

  Fragments arranged by size: 

                 505     146      90
 
BbeI G_GCGC'C

Cuts at:      0     384     741 
   Size:        384     357
 
BlpI GC'TnA_GC

Cuts at:      0     412     741 
   Size:        412     329
 
Bme1580I G_kGCm'C

Cuts at:      0     146     651     741 
   Size:        146     505      90

  Fragments arranged by size: 

                 505     146      90
 
BmgBI CAC'GTC

Cuts at:      0     272     741 
   Size:        272     469
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     612     627     741 
   Size:        612      15     114

  Fragments arranged by size: 

                 612     114      15
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     148     349     741 
   Size:        148     201     392

  Fragments arranged by size: 

                 392     201     148
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     541     741 
   Size:        541     200
 
BsaAI yAC'GTr

Cuts at:      0     195     642     741 
   Size:        195     447      99

  Fragments arranged by size: 

                 447     195      99
 
BsaHI Gr'CG_yC

Cuts at:      0     381     741 
   Size:        381     360
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     302     741 
   Size:        302     439
 
BsiWI C'GTAC_G

Cuts at:      0     638     741 
   Size:        638     103
 
Bsp1286I G_dGCh'C

Cuts at:      0     146     651     741 
   Size:        146     505      90

  Fragments arranged by size: 

                 505     146      90
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     142     741 
   Size:        142     599
 
BsrGI T'GTAC_A

Cuts at:      0     266     715     741 
   Size:        266     449      26

  Fragments arranged by size: 

                 449     266      26
 
BstBI TT'CG_AA

Cuts at:      0     188     741 
   Size:        188     553
 
BtgI C'CryG_G

Cuts at:      0     117     563     656     741 
   Size:        117     446      93      85

  Fragments arranged by size: 

                 446     117      93      85
 
BtsI GCAGTG_nn'

Cuts at:      0     473     741 
   Size:        473     268
 
EarI CTCTTCn'nnn_

Cuts at:      0     197     320     741 
   Size:        197     123     421

  Fragments arranged by size: 

                 421     197     123
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     148     349     550     741 
   Size:        148     201     201     191

  Fragments arranged by size: 

                 201     201     191     148
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     502     741 
   Size:        502     239
 
EcoO109I rG'GnC_Cy

Cuts at:      0     507     648     741 
   Size:        507     141      93

  Fragments arranged by size: 

                 507     141      93
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     555     741 
   Size:        555     186
 
HaeII r_GCGC'y

Cuts at:      0     384     741 
   Size:        384     357
 
KasI G'GCGC_C

Cuts at:      0     380     741 
   Size:        380     361
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     191     741 
   Size:        191     550
 
MslI CAynn'nnrTG

Cuts at:      0     367     741 
   Size:        367     374
 
NarI GG'CG_CC

Cuts at:      0     381     741 
   Size:        381     360
 
NcoI C'CATG_G

Cuts at:      0     117     563     656     741 
   Size:        117     446      93      85

  Fragments arranged by size: 

                 446     117      93      85
 
NspI r_CATG'y

Cuts at:      0     366     477     741 
   Size:        366     111     264

  Fragments arranged by size: 

                 366     264     111
 
PciI A'CATG_T

Cuts at:      0     362     473     741 
   Size:        362     111     268

  Fragments arranged by size: 

                 362     268     111
 
PmlI CAC'GTG

Cuts at:      0     195     741 
   Size:        195     546
 
PpuMI rG'GwC_Cy

Cuts at:      0     507     741 
   Size:        507     234
 
PspOMI G'GGCC_C

Cuts at:      0     142     647     741 
   Size:        142     505      94

  Fragments arranged by size: 

                 505     142      94
 
PstI C_TGCA'G

Cuts at:      0     468     589     741 
   Size:        468     121     152

  Fragments arranged by size: 

                 468     152     121
 
SapI GCTCTTCn'nnn_

Cuts at:      0     197     741 
   Size:        197     544
 
SfcI C'TryA_G

Cuts at:      0     464     585     741 
   Size:        464     121     156

  Fragments arranged by size: 

                 464     156     121
 
SfoI GGC'GCC

Cuts at:      0     382     741 
   Size:        382     359
 
StyI C'CwwG_G

Cuts at:      0     117     146     563     656     741 
   Size:        117      29     417      93      85

  Fragments arranged by size: 

                 417     117      93      85      29
 
TatI w'GTAC_w

Cuts at:      0     266     715     741 
   Size:        266     449      26

  Fragments arranged by size: 

                 449     266      26
 

 Enzymes that do cut:

     AcuI   AflIII     ApaI     BanI    BanII     BbeI     BlpI Bme1580I
    BmgBI     BmrI     BpmI     BsaI    BsaAI    BsaHI    BseRI    BsiWI
 Bsp1286I    BspMI    BsrGI    BstBI     BtgI     BtsI     EarI  Eco57MI
    EcoNI EcoO109I     FalI    HaeII     KasI     MmeI     MslI     NarI
     NcoI     NspI     PciI     PmlI    PpuMI   PspOMI     PstI     SapI
     SfcI     SfoI     StyI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BbsI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BmtI     BplI
   Bpu10I    BpuEI    BsaBI    BsaWI    BsaXI    BseYI     BsgI    BsiEI
  BsiHKAI     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrDI    BsrFI
   BssHII    BssSI   BstAPI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I
    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EciI  EcoICRI    EcoRI    EcoRV     FseI     FspI    FspAI    Hin4I
   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MscI   MspA1I
     NaeI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI    PflMI     PfoI     PmeI     PpiI    PshAI     PsiI    PspXI
     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SbfI     ScaI    SexAI     SfiI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI
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