Restriction map for pF1KB9777
 (Circular) (Six-base) MAPSORT of: pF1KB9777.seq  Check: 1049  from: 1  to: 5477



pF1KB9777  5477 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:31  ..

AccI GT'mk_AC

Cuts at:   2195    2515    2195 
   Size:        320    5157
 
Acc65I G'GTAC_C

Cuts at:   2493    2493 
   Size:       5477
 
AclI AA'CG_TT

Cuts at:   5317    5317 
   Size:       5477
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    115     584     979    1036    1609    3302    3734    4087    4749 
   Size:        469     395      57     573    1693     432     353     662

Cuts at:   4749     115 
   Size:        843

  Fragments arranged by size: 

                1693     843     662     573     469     432     395     353
                  57
 
AflIII A'CryG_T

Cuts at:   2829    4629    4910    2829 
   Size:       1800     281    3396

  Fragments arranged by size: 

                3396    1800     281
 
AleI CACnn'nnGTG

Cuts at:    677    1386     677 
   Size:        709    4768
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1831    1863    1831 
   Size:         32    5445
 
AlwNI CAG_nnn'CTG

Cuts at:    945    1434    2782    4220     945 
   Size:        489    1348    1438    2202

  Fragments arranged by size: 

                2202    1438    1348     489
 
ApaI G_GGCC'C

Cuts at:   1409    1409 
   Size:       5477
 
ApaLI G'TGCA_C

Cuts at:   4315    4315 
   Size:       5477
 
ApoI r'AATT_y

Cuts at:    366     414    1231    2481    4830    5086     366 
   Size:         48     817    1250    2349     256     757

  Fragments arranged by size: 

                2349    1250     817     757     256      48
 
AseI AT'TA_AT

Cuts at:     20     374      20 
   Size:        354    5123
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5477
 
AvaI C'yCGr_G

Cuts at:    202     913    1412    2081    2497     202 
   Size:        711     499     669     416    3182

  Fragments arranged by size: 

                3182     711     669     499     416
 
BamHI G'GATC_C

Cuts at:   2110    2502    2110 
   Size:        392    5085
 
BanI G'GyrC_C

Cuts at:   1137    2493    2907    3155    3190    1137 
   Size:       1356     414     248      35    3424

  Fragments arranged by size: 

                3424    1356     414     248      35
 
BanII G_rGCy'C

Cuts at:    410    1037    1409    2491    3521     410 
   Size:        627     372    1082    1030    2366

  Fragments arranged by size: 

                2366    1082    1030     627     372
 
BbeI G_GCGC'C

Cuts at:   1141    2911    3159    1141 
   Size:       1770     248    3459

  Fragments arranged by size: 

                3459    1770     248
 
BbsI GAAGACnn'nnnn_

Cuts at:    416     985    1384    1403    1597     416 
   Size:        569     399      19     194    4296

  Fragments arranged by size: 

                4296     569     399     194      19
 
BciVI GTATCCnnnnn_n'

Cuts at:   3375    4431    5477    3375 
   Size:       1056    1046    3375

  Fragments arranged by size: 

                3375    1056    1046
 
BclI T'GATC_A

Cuts at:    936    2997     936 
   Size:       2061    3416
 
BglII A'GATC_T

Cuts at:   2992    5029    2992 
   Size:       2037    3440
 
BlpI GC'TnA_GC

Cuts at:   1099    2589    1099 
   Size:       1490    3987
 
Bme1580I G_kGCm'C

Cuts at:   1409    3102    3195    4319    1409 
   Size:       1693      93    1124    2567

  Fragments arranged by size: 

                2567    1693    1124      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    849    1422    1637    2689    2770    3105    5249     849 
   Size:        573     215    1052      81     335    2144    1077

  Fragments arranged by size: 

                2144    1077    1052     573     335     215      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3844    3844 
   Size:       5477
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    335     817     985    1456    1670    1849    2487     335 
   Size:        482     168     471     214     179     638    3325

  Fragments arranged by size: 

                3325     638     482     471     214     179     168
 
Bpu10I CC'TnA_GC

Cuts at:    588     588 
   Size:       5477
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    394    2653    3532    4246    4544     394 
   Size:       2259     879     714     298    1327

  Fragments arranged by size: 

                2259    1327     879     714     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   1057    2132    5181    1057 
   Size:       1075    3049    1353

  Fragments arranged by size: 

                3049    1353    1075
 
BsaAI yAC'GTr

Cuts at:   2830    2830 
   Size:       5477
 
BsaBI GATnn'nnATC

Cuts at:      6    2991    5028       6 
   Size:       2985    2037     455

  Fragments arranged by size: 

                2985    2037     455
 
BsaHI Gr'CG_yC

Cuts at:   1138    2908    3156    3858    5354    1138 
   Size:       1770     248     702    1496    1261

  Fragments arranged by size: 

                1770    1496    1261     702     248
 
BsaWI w'CCGG_w

Cuts at:    845    2428    2666    3187    3865    4276    4423    4697     845 
   Size:       1583     238     521     678     411     147     274    1625

  Fragments arranged by size: 

                1625    1583     678     521     411     274     238     147
            
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    308     338    1663    1693    1831    1861     308 
   Size:         30    1325      30     138      30    3924

  Fragments arranged by size: 

                3924    1325     138      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    538    1115    1936    2362     538 
   Size:        577     821     426    3653

  Fragments arranged by size: 

                3653     821     577     426
 
BseYI C'CCAG_C

Cuts at:   1047    1141    1842    2902    3660    4325    4733    1047 
   Size:         94     701    1060     758     665     408    1791

  Fragments arranged by size: 

                1791    1060     758     701     665     408      94
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    907     907 
   Size:       5477
 
BsiEI CG_ry'CG

Cuts at:    104    3019    3065    4295     104 
   Size:       2915      46    1230    1286

  Fragments arranged by size: 

                2915    1286    1230      46
 
BsiHKAI G_wGCw'C

Cuts at:    410    1037    1393    1684    1730    2491    3269    3459    4319 
   Size:        627     356     291      46     761     778     190     860

Cuts at:   4319     410 
   Size:       1568

  Fragments arranged by size: 

                1568     860     778     761     627     356     291     190
                  46
 
BsmI GAATG_Cn'

Cuts at:   1058    1715    1058 
   Size:        657    4820
 
Bsp1286I G_dGCh'C

Cuts at:    410    1037    1393    1409    1684    1730    2491    3102    3195 
   Size:        627     356      16     275      46     761     611      93

Cuts at:   3195    3269    3459    3521    4319     410 
   Size:         74     190      62     798    1568

  Fragments arranged by size: 

                1568     798     761     627     611     356     275     190
                  93      74      62      46      16
 
BspEI T'CCGG_A

Cuts at:   2428    2428 
   Size:       5477
 
BspHI T'CATG_A

Cuts at:   3909    3909 
   Size:       5477
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    747    1440    2527    2741    3043    3424     747 
   Size:        693    1087     214     302     381    2800

  Fragments arranged by size: 

                2800    1087     693     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    3769    3823    5307    5472      50 
   Size:       3719      54    1484     165      55

  Fragments arranged by size: 

                3719    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    408    1944    3389     408 
   Size:       1536    1445    2496

  Fragments arranged by size: 

                2496    1536    1445
 
BsrFI r'CCGG_y

Cuts at:    735     881    3475    3656     735 
   Size:        146    2594     181    2556

  Fragments arranged by size: 

                2594    2556     181     146
 
BssSI C'ACGA_G

Cuts at:   1723    3748    4456    1723 
   Size:       2025     708    2744

  Fragments arranged by size: 

                2744    2025     708
 
BstAPI GCAn_nnn'nTGC

Cuts at:   2854    2854 
   Size:       5477
 
BstBI TT'CG_AA

Cuts at:   3838    3838 
   Size:       5477
 
BstEII G'GTnAC_C

Cuts at:    682     682 
   Size:       5477
 
BstXI CCAn_nnnn'nTGG

Cuts at:    963     963 
   Size:       5477
 
BstYI r'GATC_y

Cuts at:      1    1296    1584    2110    2425    2502    2969    2992    3327 
   Size:       1295     288     526     315      77     467      23     335

Cuts at:   3327    3573    3879    3891    3977    5029       1 
   Size:        246     306      12      86    1052     449

  Fragments arranged by size: 

                1295    1052     526     467     449     335     315     306
                 288     246      86      77      23      12
 
Bsu36I CC'TnA_GG

Cuts at:    215     512    1538     215 
   Size:        297    1026    4154

  Fragments arranged by size: 

                4154    1026     297
 
BtgI C'CryG_G

Cuts at:   1133    1133 
   Size:       5477
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   3427    3608    3771    3427 
   Size:        181     163    5133

  Fragments arranged by size: 

                5133     181     163
 
BtsI GCAGTG_nn'

Cuts at:    684     717    2015    2720     684 
   Size:         33    1298     705    3441

  Fragments arranged by size: 

                3441    1298     705      33
 
DraI TTT'AAA

Cuts at:   2268    2476    2268 
   Size:        208    5269
 
DrdI GACnn_nn'nnGTC

Cuts at:    846    3183    4527     846 
   Size:       2337    1344    1796

  Fragments arranged by size: 

                2337    1796    1344
 
EaeI y'GGCC_r

Cuts at:   1797    2172    3062    3236    3627    3654    4999    1797 
   Size:        375     890     174     391      27    1345    2275

  Fragments arranged by size: 

                2275    1345     890     391     375     174      27
 
EagI C'GGCC_G

Cuts at:   3062    3062 
   Size:       5477
 
EarI CTCTTCn'nnn_

Cuts at:    133     479     770    1012    1547    2339    3500    3710     133 
   Size:        346     291     242     535     792    1161     210    1900

  Fragments arranged by size: 

                1900    1161     792     535     346     291     242     210
            
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1977    3685    4427    4573    5287    1977 
   Size:       1708     742     146     714    2167

  Fragments arranged by size: 

                2167    1708     742     714     146
 
EcoICRI GAG'CTC

Cuts at:    408    1035    2489     408 
   Size:        627    1454    3396

  Fragments arranged by size: 

                3396    1454     627
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    115     335     584     817     979     985    1036    1456    1609 
   Size:        220     249     233     162       6      51     420     153

Cuts at:   1609    1670    1849    2487    3302    3734    4087    4749     115 
   Size:         61     179     638     815     432     353     662     843

  Fragments arranged by size: 

                 843     815     662     638     432     420     353     249
                 233     220     179     162     153      61      51       6
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:    440     440 
   Size:       5477
 
EcoO109I rG'GnC_Cy

Cuts at:    188     516     897    1405    2616     188 
   Size:        328     381     508    1211    3049

  Fragments arranged by size: 

                3049    1211     508     381     328
 
EcoRI G'AATT_C

Cuts at:    414    2481     414 
   Size:       2067    3410
 
EcoRV GAT'ATC

Cuts at:   1974    1974 
   Size:       5477
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   3496    3496 
   Size:       5477
 
FspI TGC'GCA

Cuts at:   3258    3258 
   Size:       5477
 
HaeII r_GCGC'y

Cuts at:    636    1141    1828    2911    3159    4389    5160     636 
   Size:        505     687    1083     248    1230     771     953

  Fragments arranged by size: 

                1230    1083     953     771     687     505     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1668    1700    2359    2391    2410    2442    3319    3349    3351 
   Size:         32     659      32      19      32     877      30       2

Cuts at:   3351    3381    1668 
   Size:         30    3764

  Fragments arranged by size: 

                3764     877     659      32      32      32      30      30
                  19       2
 
HincII GTy'rAC

Cuts at:   2196    2516    2196 
   Size:        320    5157
 
KasI G'GCGC_C

Cuts at:   1137    2907    3155    1137 
   Size:       1770     248    3459

  Fragments arranged by size: 

                3459    1770     248
 
KpnI G_GTAC'C

Cuts at:   2497    2497 
   Size:       5477
 
MluI A'CGCG_T

Cuts at:   4910    4910 
   Size:       5477
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    220     439    1562    4236    4420     220 
   Size:        219    1123    2674     184    1277

  Fragments arranged by size: 

                2674    1277    1123     219     184
 
MscI TGG'CCA

Cuts at:   1799    2174    3238    1799 
   Size:        375    1064    4038

  Fragments arranged by size: 

                4038    1064     375
 
MslI CAynn'nnrTG

Cuts at:    263     677    1386    3466    3593     263 
   Size:        414     709    2080     127    2147

  Fragments arranged by size: 

                2147    2080     709     414     127
 
MspA1I CmG'CkG

Cuts at:    963    1047    1179    2127    2588    2902    3262    4046    4291 
   Size:         84     132     948     461     314     360     784     245

Cuts at:   4291     963 
   Size:       2149

  Fragments arranged by size: 

                2149     948     784     461     360     314     245     132
                  84
 
NaeI GCC'GGC

Cuts at:    737     883    3658     737 
   Size:        146    2775    2556

  Fragments arranged by size: 

                2775    2556     146
 
NarI GG'CG_CC

Cuts at:   1138    2908    3156    1138 
   Size:       1770     248    3459

  Fragments arranged by size: 

                3459    1770     248
 
NgoMIV G'CCGG_C

Cuts at:    735     881    3656     735 
   Size:        146    2775    2556

  Fragments arranged by size: 

                2775    2556     146
 
NspI r_CATG'y

Cuts at:   2530    3030    4633    2530 
   Size:        500    1603    3374

  Fragments arranged by size: 

                3374    1603     500
 
PasI CC'CwG_GG

Cuts at:    355     355 
   Size:       5477
 
PciI A'CATG_T

Cuts at:   4629    4629 
   Size:       5477
 
PflMI CCAn_nnn'nTGG

Cuts at:   1434    1742    1434 
   Size:        308    5169
 
PmeI GTTT'AAAC

Cuts at:   2476    2476 
   Size:       5477
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1831    1863    3923    3955    1831 
   Size:         32    2060      32    3353

  Fragments arranged by size: 

                3353    2060      32      32
 
PpuMI rG'GwC_Cy

Cuts at:    188     516     897     188 
   Size:        328     381    4768

  Fragments arranged by size: 

                4768     381     328
 
PspOMI G'GGCC_C

Cuts at:   1405    1405 
   Size:       5477
 
PspXI vC'TCGA_Gb

Cuts at:    913     913 
   Size:       5477
 
PstI C_TGCA'G

Cuts at:    276     758    1451    1820    2524    3209     276 
   Size:        482     693     369     704     685    2544

  Fragments arranged by size: 

                2544     704     693     685     482     369
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       5477
 
PvuII CAG'CTG

Cuts at:    963    1047    1179    2902    3262     963 
   Size:         84     132    1723     360    3178

  Fragments arranged by size: 

                3178    1723     360     132      84
 
RsrII CG'GwC_CG

Cuts at:   3672    3672 
   Size:       5477
 
SacI G_AGCT'C

Cuts at:    410    1037    2491     410 
   Size:        627    1454    3396

  Fragments arranged by size: 

                3396    1454     627
 
SalI G'TCGA_C

Cuts at:   2194    2514    2194 
   Size:        320    5157
 
SanDI GG'GwC_CC

Cuts at:    897     897 
   Size:       5477
 
SapI GCTCTTCn'nnn_

Cuts at:    770    1012    3500    3710     770 
   Size:        242    2488     210    2537

  Fragments arranged by size: 

                2537    2488     242     210
 
SbfI CC_TGCA'GG

Cuts at:   2524    2524 
   Size:       5477
 
ScaI AGT'ACT

Cuts at:   4994    4994 
   Size:       5477
 
SfcI C'TryA_G

Cuts at:     33     272     754    1207    1447    1816    2520    3205    4173 
   Size:        239     482     453     240     369     704     685     968

Cuts at:   4173    4364      33 
   Size:        191    1146

  Fragments arranged by size: 

                1146     968     704     685     482     453     369     240
                 239     191
 
SfoI GGC'GCC

Cuts at:   1139    2909    3157    1139 
   Size:       1770     248    3459

  Fragments arranged by size: 

                3459    1770     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5477
 
SmaI CCC'GGG

Cuts at:    204    1414    2499     204 
   Size:       1210    1085    3182

  Fragments arranged by size: 

                3182    1210    1085
 
SmlI C'TyrA_G

Cuts at:    409     913    2081    2632    3547    4261    4523     409 
   Size:        504    1168     551     915     714     262    1363

  Fragments arranged by size: 

                1363    1168     915     714     551     504     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       5477
 
SphI G_CATG'C

Cuts at:   2530    3030    2530 
   Size:        500    4977
 
SspI AAT'ATT

Cuts at:    151     151 
   Size:       5477
 
StuI AGG'CCT

Cuts at:   1991    1991 
   Size:       5477
 
StyI C'CwwG_G

Cuts at:   1076    1568    1736    2611    2965    1076 
   Size:        492     168     875     354    3588

  Fragments arranged by size: 

                3588     875     492     354     168
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    275    3060    3193    3652    3861     275 
   Size:       2785     133     459     209    1891

  Fragments arranged by size: 

                2785    1891     459     209     133
 
TatI w'GTAC_w

Cuts at:    534    1657    2186    2336    4992     534 
   Size:       1123     529     150    2656    1019

  Fragments arranged by size: 

                2656    1123    1019     529     150
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    308     340    1663    1695    1737    1769    2102    2134    4994 
   Size:         32    1323      32      42      32     333      32    2860

Cuts at:   4994    5026     308 
   Size:         32     759

  Fragments arranged by size: 

                2860    1323     759     333      42      32      32      32
                  32      32
 
Tth111I GACn'n_nGTC

Cuts at:    169    1084    3274     169 
   Size:        915    2190    2372

  Fragments arranged by size: 

                2372    2190     915
 
XbaI T'CTAG_A

Cuts at:   2508    2508 
   Size:       5477
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1176    2972    1176 
   Size:       1796    3681
 
XhoI C'TCGA_G

Cuts at:    913    2081     913 
   Size:       1168    4309
 
XmaI C'CCGG_G

Cuts at:    202    1412    2497     202 
   Size:       1210    1085    3182

  Fragments arranged by size: 

                3182    1210    1085
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AleI     AloI    AlwNI
     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI
    BanII     BbeI     BbsI    BciVI     BclI    BglII     BlpI Bme1580I
     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI   Bsu36I     BtgI
    BtgZI     BtsI     DraI     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FspI
    HaeII    Hin4I   HincII     KasI     KpnI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI   NgoMIV     NspI     PasI     PciI
    PflMI     PmeI     PpiI    PpuMI   PspOMI    PspXI     PstI     PvuI
    PvuII    RsrII     SacI     SalI    SanDI     SapI     SbfI     ScaI
     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     SspI
     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AgeI     AhdI     AlfI     AscI
    AvrII     BaeI    BbvCI     BcgI    BfrBI     BglI    BmgBI     BmtI
    BsiWI    BsmBI    BsrGI   BssHII  BstZ17I     ClaI    CspCI   DraIII
     FseI    FspAI  HindIII     HpaI     MfeI     NcoI     NdeI     NheI
     NotI     NruI     NsiI     PacI     PfoI     PmlI    PshAI     PsiI
     PsrI    SacII    SexAI     SfiI    SgrAI    SnaBI     SrfI     SwaI
     XmnI     ZraI
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