Restriction map for pF1KB9776
 (Circular) (Six-base) MAPSORT of: pF1KB9776.seq  Check: 5573  from: 1  to: 5261



pF1KB9776  5261 bp

 With 182 enzymes: SgfI * 

                        September 18, 2009 17:01  ..

AccI GT'mk_AC

Cuts at:   2299    2299 
   Size:       5261
 
Acc65I G'GTAC_C

Cuts at:   2277    2277 
   Size:       5261
 
AclI AA'CG_TT

Cuts at:   5101    5101 
   Size:       5261
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    795    3086    3518    4265    4533     795 
   Size:       2291     432     747     268    1523

  Fragments arranged by size: 

                2291    1523     747     432     268
 
AfeI AGC'GCT

Cuts at:   1978    1978 
   Size:       5261
 
AflIII A'CryG_T

Cuts at:   2613    3717    4694    2613 
   Size:       1104     977    3180

  Fragments arranged by size: 

                3180    1104     977
 
AgeI A'CCGG_T

Cuts at:   3649    3649 
   Size:       5261
 
AhdI GACnn_n'nnGTC

Cuts at:   1896    1896 
   Size:       5261
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1960    1960 
   Size:       5261
 
AlwNI CAG_nnn'CTG

Cuts at:   2566    4133    2566 
   Size:       1567    3694
 
ApaI G_GGCC'C

Cuts at:   1091    2141    1091 
   Size:       1050    4211
 
ApaLI G'TGCA_C

Cuts at:   1075    4031    1075 
   Size:       2956    2305
 
ApoI r'AATT_y

Cuts at:   2265    4614    4870    2265 
   Size:       2349     256    2656

  Fragments arranged by size: 

                2656    2349     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       5261
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5261
 
AvaI C'yCGr_G

Cuts at:   1686    1758    2058    2281    1686 
   Size:         72     300     223    4666

  Fragments arranged by size: 

                4666     300     223      72
 
BamHI G'GATC_C

Cuts at:   2286    2286 
   Size:       5261
 
BanI G'GyrC_C

Cuts at:   1216    1648    1965    2277    2691    2939    2974    1216 
   Size:        432     317     312     414     248      35    3503

  Fragments arranged by size: 

                3503     432     414     317     312     248      35
 
BanII G_rGCy'C

Cuts at:    358    1091    1163    1243    2141    2151    2275    3305     358 
   Size:        733      72      80     898      10     124    1030    2314

  Fragments arranged by size: 

                2314    1030     898     733     124      80      72      10
            
 
BbeI G_GCGC'C

Cuts at:   1652    1969    2695    2943    1652 
   Size:        317     726     248    3970

  Fragments arranged by size: 

                3970     726     317     248
 
BbsI GAAGACnn'nnnn_

Cuts at:    759     759 
   Size:       5261
 
BbvCI CC'TCA_GC

Cuts at:    350     350 
   Size:       5261
 
BciVI GTATCCnnnnn_n'

Cuts at:   3159    3920    5261    3159 
   Size:        761    1341    3159

  Fragments arranged by size: 

                3159    1341     761
 
BclI T'GATC_A

Cuts at:   2781    2781 
   Size:       5261
 
BglI GCCn_nnn'nGGC

Cuts at:   1167    1167 
   Size:       5261
 
BglII A'GATC_T

Cuts at:    284    2776    4813     284 
   Size:       2492    2037     732

  Fragments arranged by size: 

                2492    2037     732
 
BlpI GC'TnA_GC

Cuts at:   2373    2373 
   Size:       5261
 
Bme1580I G_kGCm'C

Cuts at:   1079    1091    2084    2141    2180    2886    2979    4035    1079 
   Size:         12     993      57      39     706      93    1056    2305

  Fragments arranged by size: 

                2305    1056     993     706      93      57      39      12
            
 
BmrI ACTGGGnnnn_n'

Cuts at:   2225    2473    2554    2889    5033    2225 
   Size:        248      81     335    2144    2453

  Fragments arranged by size: 

                2453    2144     335     248      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    303     531     711     819    3628     303 
   Size:        228     180     108    2809    1936

  Fragments arranged by size: 

                2809    1936     228     180     108
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    679    1626     679 
   Size:        947    4314
 
Bpu10I CC'TnA_GC

Cuts at:    350    1249    1980     350 
   Size:        899     731    3631

  Fragments arranged by size: 

                3631     899     731
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1393    2437    3316    3808    4106    1393 
   Size:       1044     879     492     298    2548

  Fragments arranged by size: 

                2548    1044     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    109     655    1197    4965     109 
   Size:        546     542    3768     405

  Fragments arranged by size: 

                3768     546     542     405
 
BsaAI yAC'GTr

Cuts at:   1132    2614    1132 
   Size:       1482    3779
 
BsaBI GATnn'nnATC

Cuts at:      6    1417    2775    4812       6 
   Size:       1411    1358    2037     455

  Fragments arranged by size: 

                2037    1411    1358     455
 
BsaHI Gr'CG_yC

Cuts at:    151    1649    1966    2692    2940    3642    5138     151 
   Size:       1498     317     726     248     702    1496     274

  Fragments arranged by size: 

                1498    1496     726     702     317     274     248
 
BsaWI w'CCGG_w

Cuts at:   1487    2450    2971    3649    3923    4070    4481    1487 
   Size:        963     521     678     274     147     411    2267

  Fragments arranged by size: 

                2267     963     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    139     169    1624    1654     139 
   Size:         30    1455      30    3746

  Fragments arranged by size: 

                3746    1455      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    295    1263     295 
   Size:        968    4293
 
BseYI C'CCAG_C

Cuts at:    226     369     530    1115    1773    1969    2686    3444    4021 
   Size:        143     161     585     658     196     717     758     577

Cuts at:   4021    4517     226 
   Size:        496     970

  Fragments arranged by size: 

                 970     758     717     658     585     577     496     196
                 161     143
 
BsiEI CG_ry'CG

Cuts at:    104    1860    2803    2849    4057     104 
   Size:       1756     943      46    1208    1308

  Fragments arranged by size: 

                1756    1308    1208     943      46
 
BsiHKAI G_wGCw'C

Cuts at:   1079    1255    2003    2275    3053    3243    4035    1079 
   Size:        176     748     272     778     190     792    2305

  Fragments arranged by size: 

                2305     792     778     748     272     190     176
 
BsmBI CGTCTCn'nnnn_

Cuts at:    142     502     142 
   Size:        360    4901
 
Bsp1286I G_dGCh'C

Cuts at:    358    1079    1091    1163    1243    1255    2003    2084    2141 
   Size:        721      12      72      80      12     748      81      57

Cuts at:   2141    2151    2180    2275    2886    2979    3053    3243    3305 
   Size:         10      29      95     611      93      74     190      62

Cuts at:   3305    4035     358 
   Size:        730    1584

  Fragments arranged by size: 

                1584     748     730     721     611     190      95      93
                  81      80      74      72      62      57      29      12
                  12      10
 
BspEI T'CCGG_A

Cuts at:   4481    4481 
   Size:       5261
 
BspHI T'CATG_A

Cuts at:    950    4437     950 
   Size:       3487    1774
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    695    1526    2311    2525    2827    3208     695 
   Size:        831     785     214     302     381    2748

  Fragments arranged by size: 

                2748     831     785     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50     540     798    1962    3553    3607    5091    5256      50 
   Size:        490     258    1164    1591      54    1484     165      55

  Fragments arranged by size: 

                1591    1484    1164     490     258     165      55      54
            
 
BsrDI GCAATG_nn'

Cuts at:   1144    3173    1144 
   Size:       2029    3232
 
BsrFI r'CCGG_y

Cuts at:   1213    1986    3259    3440    3649    1213 
   Size:        773    1273     181     209    2825

  Fragments arranged by size: 

                2825    1273     773     209     181
 
BssSI C'ACGA_G

Cuts at:   3532    3890    3532 
   Size:        358    4903
 
BstAPI GCAn_nnn'nTGC

Cuts at:   2638    2638 
   Size:       5261
 
BstBI TT'CG_AA

Cuts at:   3622    3622 
   Size:       5261
 
BstEII G'GTnAC_C

Cuts at:   1591    1591 
   Size:       5261
 
BstXI CCAn_nnnn'nTGG

Cuts at:    139     139 
   Size:       5261
 
BstYI r'GATC_y

Cuts at:      1     284    2286    2753    2776    3111    3357    4369    4455 
   Size:        283    2002     467      23     335     246    1012      86

Cuts at:   4455    4467    4813       1 
   Size:         12     346     449

  Fragments arranged by size: 

                2002    1012     467     449     346     335     283     246
                  86      23      12
 
BtgI C'CryG_G

Cuts at:    107     965    1361    1706    1954    2126    2132    2219     107 
   Size:        858     396     345     248     172       6      87    3149

  Fragments arranged by size: 

                3149     858     396     345     248     172      87       6
            
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   3211    3392    3555    3211 
   Size:        181     163    4917

  Fragments arranged by size: 

                4917     181     163
 
BtsI GCAGTG_nn'

Cuts at:    657     738    1199    1579    1839    2504     657 
   Size:         81     461     380     260     665    3414

  Fragments arranged by size: 

                3414     665     461     380     260      81
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    900     935     900 
   Size:         35    5226
 
DraI TTT'AAA

Cuts at:   2260    2260 
   Size:       5261
 
DraIII CAC_nnn'GTG

Cuts at:   1470    1470 
   Size:       5261
 
DrdI GACnn_nn'nnGTC

Cuts at:   1051    2967    3825    1051 
   Size:       1916     858    2487

  Fragments arranged by size: 

                2487    1916     858
 
EaeI y'GGCC_r

Cuts at:    321     541    1610    1991    2027    2129    2216    2846    3020 
   Size:        220    1069     381      36     102      87     630     174

Cuts at:   3020    3411    3438    4783     321 
   Size:        391      27    1345     799

  Fragments arranged by size: 

                1345    1069     799     630     391     381     220     174
                 102      87      36      27
 
EagI C'GGCC_G

Cuts at:   2846    2846 
   Size:       5261
 
EarI CTCTTCn'nnn_

Cuts at:   3284    3494    3284 
   Size:        210    5051
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    214     786    1528    3469    3779    3925    5071     214 
   Size:        572     742    1941     310     146    1146     404

  Fragments arranged by size: 

                1941    1146     742     572     404     310     146
 
EcoICRI GAG'CTC

Cuts at:   2273    2273 
   Size:       5261
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    679     795    1626    3086    3518    4265    4533     679 
   Size:        116     831    1460     432     747     268    1407

  Fragments arranged by size: 

                1460    1407     831     747     432     268     116
 
EcoNI CCTnn'n_nnAGG

Cuts at:   1376    1376 
   Size:       5261
 
EcoO109I rG'GnC_Cy

Cuts at:   1087    1119    1875    2137    2138    2400    1087 
   Size:         32     756     262       1     262    3948

  Fragments arranged by size: 

                3948     756     262     262      32       1
 
EcoRI G'AATT_C

Cuts at:   2265    2265 
   Size:       5261
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    258    1578    3280     258 
   Size:       1320    1702    2239

  Fragments arranged by size: 

                2239    1702    1320
 
FspI TGC'GCA

Cuts at:   3042    3042 
   Size:       5261
 
HaeII r_GCGC'y

Cuts at:   1308    1652    1802    1969    1980    2695    2943    3965    4944 
   Size:        344     150     167      11     715     248    1022     979

Cuts at:   4944    1308 
   Size:       1625

  Fragments arranged by size: 

                1625    1022     979     715     344     248     167     150
                  11
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    379     411     619     651    1882    1914    2052    2084    2155 
   Size:         32     208      32    1231      32     138      32      71

Cuts at:   2155    2187    3103    3133    3135    3165     379 
   Size:         32     916      30       2      30    2475

  Fragments arranged by size: 

                2475    1231     916     208     138      71      32      32
                  32      32      32      30      30       2
 
HincII GTy'rAC

Cuts at:   1551    2300    1551 
   Size:        749    4512
 
KasI G'GCGC_C

Cuts at:   1648    1965    2691    2939    1648 
   Size:        317     726     248    3970

  Fragments arranged by size: 

                3970     726     317     248
 
KpnI G_GTAC'C

Cuts at:   2281    2281 
   Size:       5261
 
MluI A'CGCG_T

Cuts at:   4694    4694 
   Size:       5261
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1364    1807    1865    3932    4116    1364 
   Size:        443      58    2067     184    2509

  Fragments arranged by size: 

                2509    2067     443     184      58
 
MscI TGG'CCA

Cuts at:   1612    2029    2218    3022    1612 
   Size:        417     189     804    3851

  Fragments arranged by size: 

                3851     804     417     189
 
MslI CAynn'nnrTG

Cuts at:    137     464     470     643    2107    2218    3250    3377     137 
   Size:        327       6     173    1464     111    1032     127    2021

  Fragments arranged by size: 

                2021    1464    1032     327     173     127     111       6
            
 
MspA1I CmG'CkG

Cuts at:    349    1910    1956    2128    2372    2686    3046    4059    4304 
   Size:       1561      46     172     244     314     360    1013     245

Cuts at:   4304     349 
   Size:       1306

  Fragments arranged by size: 

                1561    1306    1013     360     314     245     244     172
                  46
 
NaeI GCC'GGC

Cuts at:   1988    3442    1988 
   Size:       1454    3807
 
NarI GG'CG_CC

Cuts at:   1649    1966    2692    2940    1649 
   Size:        317     726     248    3970

  Fragments arranged by size: 

                3970     726     317     248
 
NcoI C'CATG_G

Cuts at:    107    1361    1706    2132    2219     107 
   Size:       1254     345     426      87    3149

  Fragments arranged by size: 

                3149    1254     426     345      87
 
NgoMIV G'CCGG_C

Cuts at:   1986    3440    1986 
   Size:       1454    3807
 
NspI r_CATG'y

Cuts at:    571    1404    2314    2814    3721     571 
   Size:        833     910     500     907    2111

  Fragments arranged by size: 

                2111     910     907     833     500
 
PciI A'CATG_T

Cuts at:   3717    3717 
   Size:       5261
 
PflMI CCAn_nnn'nTGG

Cuts at:   1115    1470    2025    1115 
   Size:        355     555    4351

  Fragments arranged by size: 

                4351     555     355
 
PfoI T'CCnGG_A

Cuts at:   1766    2073    1766 
   Size:        307    4954
 
PmeI GTTT'AAAC

Cuts at:   2260    2260 
   Size:       5261
 
PmlI CAC'GTG

Cuts at:   1132    1132 
   Size:       5261
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4400    4432    4400 
   Size:         32    5229
 
PpuMI rG'GwC_Cy

Cuts at:   1119    1875    1119 
   Size:        756    4505
 
PshAI GACnn'nnGTC

Cuts at:   1124    1124 
   Size:       5261
 
PspOMI G'GGCC_C

Cuts at:   1087    2137    1087 
   Size:       1050    4211
 
PstI C_TGCA'G

Cuts at:   1206    2308    2993    1206 
   Size:       1102     685    3474

  Fragments arranged by size: 

                3474    1102     685
 
PvuI CG_AT'CG

Cuts at:    104    1860     104 
   Size:       1756    3505
 
PvuII CAG'CTG

Cuts at:    349    2686    3046     349 
   Size:       2337     360    2564

  Fragments arranged by size: 

                2564    2337     360
 
RsrII CG'GwC_CG

Cuts at:   1485    3456    1485 
   Size:       1971    3290
 
SacI G_AGCT'C

Cuts at:   2275    2275 
   Size:       5261
 
SacII CC_GC'GG

Cuts at:   1957    2129    1957 
   Size:        172    5089
 
SalI G'TCGA_C

Cuts at:   2298    2298 
   Size:       5261
 
SanDI GG'GwC_CC

Cuts at:   1119    1119 
   Size:       5261
 
SapI GCTCTTCn'nnn_

Cuts at:   3284    3494    3284 
   Size:        210    5051
 
SbfI CC_TGCA'GG

Cuts at:   2308    2308 
   Size:       5261
 
ScaI AGT'ACT

Cuts at:   4778    4778 
   Size:       5261
 
SfcI C'TryA_G

Cuts at:     33    1103    1202    1779    2304    2989    3982    4173      33 
   Size:       1070      99     577     525     685     993     191    1121

  Fragments arranged by size: 

                1121    1070     993     685     577     525     191      99
            
 
SfoI GGC'GCC

Cuts at:   1650    1967    2693    2941    1650 
   Size:        317     726     248    3970

  Fragments arranged by size: 

                3970     726     317     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5261
 
SmaI CCC'GGG

Cuts at:   1688    1760    2283    1688 
   Size:         72     523    4666

  Fragments arranged by size: 

                4666     523      72
 
SmlI C'TyrA_G

Cuts at:   1408    2416    3331    3823    4085    1408 
   Size:       1008     915     492     262    2584

  Fragments arranged by size: 

                2584    1008     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       5261
 
SphI G_CATG'C

Cuts at:    571    2314    2814     571 
   Size:       1743     500    3018

  Fragments arranged by size: 

                3018    1743     500
 
StyI C'CwwG_G

Cuts at:    107     194    1361    1699    1706    2132    2219    2395    2749 
   Size:         87    1167     338       7     426      87     176     354

Cuts at:   2749     107 
   Size:       2619

  Fragments arranged by size: 

                2619    1167     426     354     338     176      87      87
                   7
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    377    1757    2844    2977    3436    3645     377 
   Size:       1380    1087     133     459     209    1993

  Fragments arranged by size: 

                1993    1380    1087     459     209     133
 
TatI w'GTAC_w

Cuts at:   4776    4776 
   Size:       5261
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1624    1656    4778    4810    1624 
   Size:         32    3122      32    2075

  Fragments arranged by size: 

                3122    2075      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   3058    3058 
   Size:       5261
 
XbaI T'CTAG_A

Cuts at:   2292    2292 
   Size:       5261
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    466    1633    2756     466 
   Size:       1167    1123    2971

  Fragments arranged by size: 

                2971    1167    1123
 
XmaI C'CCGG_G

Cuts at:   1686    1758    2281    1686 
   Size:         72     523    4666

  Fragments arranged by size: 

                4666     523      72
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI     AfeI   AflIII     AgeI     AhdI
     AlfI    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI
    BamHI     BanI    BanII     BbeI     BbsI    BbvCI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I     BmrI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI   BstEII    BstXI
    BstYI     BtgI    BtgZI     BtsI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI     FalI     FspI    HaeII    Hin4I   HincII     KasI     KpnI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
   NgoMIV     NspI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI   PspOMI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI     SfcI     SfoI
     SgfI     SmaI     SmlI     SpeI     SphI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII    AflII     AleI     AloI     AscI    AvrII     BaeI
     BcgI    BfrBI    BmgBI     BmtI     BsgI    BsiWI     BsmI    BsrGI
   BssHII  BstZ17I   Bsu36I     ClaI    EcoRV     FseI    FspAI  HindIII
     HpaI     MfeI     NdeI     NheI     NotI     NruI     NsiI     PacI
     PasI     PsiI    PspXI     PsrI    SexAI     SfiI    SgrAI    SnaBI
     SrfI     SspI     StuI     SwaI     XhoI     XmnI     ZraI
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