Restriction map for pF1KB9776
 (Linear) (Six-base) MAPSORT of: KIBB9776.seq  Check: 6395  from: 1  to: 2148



KIBB9776.orf

 With 182 enzymes: SgfI * 

                        September 18, 2009 17:01  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     687    2148 
   Size:        687    1461
 
AfeI AGC'GCT

Cuts at:      0    1870    2148 
   Size:       1870     278
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1788    2148 
   Size:       1788     360
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1852    2148 
   Size:       1852     296
 
ApaI G_GGCC'C

Cuts at:      0     983    2033    2148 
   Size:        983    1050     115

  Fragments arranged by size: 

                1050     983     115
 
ApaLI G'TGCA_C

Cuts at:      0     967    2148 
   Size:        967    1181
 
AvaI C'yCGr_G

Cuts at:      0    1578    1650    1950    2148 
   Size:       1578      72     300     198

  Fragments arranged by size: 

                1578     300     198      72
 
BanI G'GyrC_C

Cuts at:      0    1108    1540    1857    2148 
   Size:       1108     432     317     291

  Fragments arranged by size: 

                1108     432     317     291
 
BanII G_rGCy'C

Cuts at:      0     250     983    1055    1135    2033    2043    2148 
   Size:        250     733      72      80     898      10     105

  Fragments arranged by size: 

                 898     733     250     105      80      72      10
 
BbeI G_GCGC'C

Cuts at:      0    1544    1861    2148 
   Size:       1544     317     287

  Fragments arranged by size: 

                1544     317     287
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     651    2148 
   Size:        651    1497
 
BbvCI CC'TCA_GC

Cuts at:      0     242    2148 
   Size:        242    1906
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1059    2148 
   Size:       1059    1089
 
BglII A'GATC_T

Cuts at:      0     176    2148 
   Size:        176    1972
 
Bme1580I G_kGCm'C

Cuts at:      0     971     983    1976    2033    2072    2148 
   Size:        971      12     993      57      39      76

  Fragments arranged by size: 

                 993     971      76      57      39      12
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    2117    2148 
   Size:       2117      31
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     195     423     603     711    2148 
   Size:        195     228     180     108    1437

  Fragments arranged by size: 

                1437     228     195     180     108
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     571    1518    2148 
   Size:        571     947     630

  Fragments arranged by size: 

                 947     630     571
 
Bpu10I CC'TnA_GC

Cuts at:      0     242    1141    1872    2148 
   Size:        242     899     731     276

  Fragments arranged by size: 

                 899     731     276     242
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1285    2148 
   Size:       1285     863
 
BsaI GGTCTCn'nnnn_

Cuts at:      0       1     547    1089    2148 
   Size:          1     546     542    1059

  Fragments arranged by size: 

                1059     546     542       1
 
BsaAI yAC'GTr

Cuts at:      0    1024    2148 
   Size:       1024    1124
 
BsaBI GATnn'nnATC

Cuts at:      0    1309    2148 
   Size:       1309     839
 
BsaHI Gr'CG_yC

Cuts at:      0      43    1541    1858    2148 
   Size:         43    1498     317     290

  Fragments arranged by size: 

                1498     317     290      43
 
BsaWI w'CCGG_w

Cuts at:      0    1379    2148 
   Size:       1379     769
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      31      61    1516    1546    2148 
   Size:         31      30    1455      30     602

  Fragments arranged by size: 

                1455     602      31      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     187    1155    2148 
   Size:        187     968     993

  Fragments arranged by size: 

                 993     968     187
 
BseYI C'CCAG_C

Cuts at:      0     118     261     422    1007    1665    1861    2148 
   Size:        118     143     161     585     658     196     287

  Fragments arranged by size: 

                 658     585     287     196     161     143     118
 
BsiEI CG_ry'CG

Cuts at:      0    1752    2148 
   Size:       1752     396
 
BsiHKAI G_wGCw'C

Cuts at:      0     971    1147    1895    2148 
   Size:        971     176     748     253

  Fragments arranged by size: 

                 971     748     253     176
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      34     394    2148 
   Size:         34     360    1754

  Fragments arranged by size: 

                1754     360      34
 
Bsp1286I G_dGCh'C

Cuts at:      0     250     971     983    1055    1135    1147    1895    1976 
   Size:        250     721      12      72      80      12     748      81

Cuts at:   1976    2033    2043    2072    2148 
   Size:         57      10      29      76

  Fragments arranged by size: 

                 748     721     250      81      80      76      72      57
                  29      12      12      10
 
BspHI T'CATG_A

Cuts at:      0     842    2148 
   Size:        842    1306
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     587    1418    2148 
   Size:        587     831     730

  Fragments arranged by size: 

                 831     730     587
 
BsrBI CCG'CTC

Cuts at:      0     432     690    1854    2148 
   Size:        432     258    1164     294

  Fragments arranged by size: 

                1164     432     294     258
 
BsrDI GCAATG_nn'

Cuts at:      0    1036    2148 
   Size:       1036    1112
 
BsrFI r'CCGG_y

Cuts at:      0    1105    1878    2148 
   Size:       1105     773     270

  Fragments arranged by size: 

                1105     773     270
 
BstEII G'GTnAC_C

Cuts at:      0    1483    2148 
   Size:       1483     665
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      31    2148 
   Size:         31    2117
 
BstYI r'GATC_y

Cuts at:      0     176    2148 
   Size:        176    1972
 
BtgI C'CryG_G

Cuts at:      0     857    1253    1598    1846    2018    2024    2111    2148 
   Size:        857     396     345     248     172       6      87      37

  Fragments arranged by size: 

                 857     396     345     248     172      87      37       6
            
 
BtsI GCAGTG_nn'

Cuts at:      0     549     630    1091    1471    1731    2148 
   Size:        549      81     461     380     260     417

  Fragments arranged by size: 

                 549     461     417     380     260      81
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     792     827    2148 
   Size:        792      35    1321

  Fragments arranged by size: 

                1321     792      35
 
DraIII CAC_nnn'GTG

Cuts at:      0    1362    2148 
   Size:       1362     786
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     943    2148 
   Size:        943    1205
 
EaeI y'GGCC_r

Cuts at:      0     213     433    1502    1883    1919    2021    2108    2148 
   Size:        213     220    1069     381      36     102      87      40

  Fragments arranged by size: 

                1069     381     220     213     102      87      40      36
            
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     106     678    1420    2148 
   Size:        106     572     742     728

  Fragments arranged by size: 

                 742     728     572     106
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     571     687    1518    2148 
   Size:        571     116     831     630

  Fragments arranged by size: 

                 831     630     571     116
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1268    2148 
   Size:       1268     880
 
EcoO109I rG'GnC_Cy

Cuts at:      0     979    1011    1767    2029    2030    2148 
   Size:        979      32     756     262       1     118

  Fragments arranged by size: 

                 979     756     262     118      32       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     150    1470    2148 
   Size:        150    1320     678

  Fragments arranged by size: 

                1320     678     150
 
HaeII r_GCGC'y

Cuts at:      0    1200    1544    1694    1861    1872    2148 
   Size:       1200     344     150     167      11     276

  Fragments arranged by size: 

                1200     344     276     167     150      11
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     271     303     511     543    1774    1806    1944    1976 
   Size:        271      32     208      32    1231      32     138      32

Cuts at:   1976    2047    2079    2148 
   Size:         71      32      69

  Fragments arranged by size: 

                1231     271     208     138      71      69      32      32
                  32      32      32
 
HincII GTy'rAC

Cuts at:      0    1443    2148 
   Size:       1443     705
 
KasI G'GCGC_C

Cuts at:      0    1540    1857    2148 
   Size:       1540     317     291

  Fragments arranged by size: 

                1540     317     291
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1256    1699    1757    2148 
   Size:       1256     443      58     391

  Fragments arranged by size: 

                1256     443     391      58
 
MscI TGG'CCA

Cuts at:      0    1504    1921    2110    2148 
   Size:       1504     417     189      38

  Fragments arranged by size: 

                1504     417     189      38
 
MslI CAynn'nnrTG

Cuts at:      0      29     356     362     535    1999    2110    2148 
   Size:         29     327       6     173    1464     111      38

  Fragments arranged by size: 

                1464     327     173     111      38      29       6
 
MspA1I CmG'CkG

Cuts at:      0     241    1802    1848    2020    2148 
   Size:        241    1561      46     172     128

  Fragments arranged by size: 

                1561     241     172     128      46
 
NaeI GCC'GGC

Cuts at:      0    1880    2148 
   Size:       1880     268
 
NarI GG'CG_CC

Cuts at:      0    1541    1858    2148 
   Size:       1541     317     290

  Fragments arranged by size: 

                1541     317     290
 
NcoI C'CATG_G

Cuts at:      0    1253    1598    2024    2111    2148 
   Size:       1253     345     426      87      37

  Fragments arranged by size: 

                1253     426     345      87      37
 
NgoMIV G'CCGG_C

Cuts at:      0    1878    2148 
   Size:       1878     270
 
NspI r_CATG'y

Cuts at:      0     463    1296    2148 
   Size:        463     833     852

  Fragments arranged by size: 

                 852     833     463
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1007    1362    1917    2148 
   Size:       1007     355     555     231

  Fragments arranged by size: 

                1007     555     355     231
 
PfoI T'CCnGG_A

Cuts at:      0    1658    1965    2148 
   Size:       1658     307     183

  Fragments arranged by size: 

                1658     307     183
 
PmlI CAC'GTG

Cuts at:      0    1024    2148 
   Size:       1024    1124
 
PpuMI rG'GwC_Cy

Cuts at:      0    1011    1767    2148 
   Size:       1011     756     381

  Fragments arranged by size: 

                1011     756     381
 
PshAI GACnn'nnGTC

Cuts at:      0    1016    2148 
   Size:       1016    1132
 
PspOMI G'GGCC_C

Cuts at:      0     979    2029    2148 
   Size:        979    1050     119

  Fragments arranged by size: 

                1050     979     119
 
PstI C_TGCA'G

Cuts at:      0    1098    2148 
   Size:       1098    1050
 
PvuI CG_AT'CG

Cuts at:      0    1752    2148 
   Size:       1752     396
 
PvuII CAG'CTG

Cuts at:      0     241    2148 
   Size:        241    1907
 
RsrII CG'GwC_CG

Cuts at:      0    1377    2148 
   Size:       1377     771
 
SacII CC_GC'GG

Cuts at:      0    1849    2021    2148 
   Size:       1849     172     127

  Fragments arranged by size: 

                1849     172     127
 
SanDI GG'GwC_CC

Cuts at:      0    1011    2148 
   Size:       1011    1137
 
SfcI C'TryA_G

Cuts at:      0     995    1094    1671    2148 
   Size:        995      99     577     477

  Fragments arranged by size: 

                 995     577     477      99
 
SfoI GGC'GCC

Cuts at:      0    1542    1859    2148 
   Size:       1542     317     289

  Fragments arranged by size: 

                1542     317     289
 
SmaI CCC'GGG

Cuts at:      0    1580    1652    2148 
   Size:       1580      72     496

  Fragments arranged by size: 

                1580     496      72
 
SmlI C'TyrA_G

Cuts at:      0    1300    2148 
   Size:       1300     848
 
SphI G_CATG'C

Cuts at:      0     463    2148 
   Size:        463    1685
 
StyI C'CwwG_G

Cuts at:      0      86    1253    1591    1598    2024    2111    2148 
   Size:         86    1167     338       7     426      87      37

  Fragments arranged by size: 

                1167     426     338      87      86      37       7
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     269    1649    2148 
   Size:        269    1380     499

  Fragments arranged by size: 

                1380     499     269
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1516    1548    2148 
   Size:       1516      32     600

  Fragments arranged by size: 

                1516     600      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     358    1525    2148 
   Size:        358    1167     623

  Fragments arranged by size: 

                1167     623     358
 
XmaI C'CCGG_G

Cuts at:      0    1578    1650    2148 
   Size:       1578      72     498

  Fragments arranged by size: 

                1578     498      72
 

 Enzymes that do cut:

     AcuI     AfeI     AhdI     AlfI     ApaI    ApaLI     AvaI     BanI
    BanII     BbeI     BbsI    BbvCI     BglI    BglII Bme1580I     BmrI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI    BsmBI Bsp1286I
    BspHI    BspMI    BsrBI    BsrDI    BsrFI   BstEII    BstXI    BstYI
     BtgI     BtsI    CspCI   DraIII     DrdI     EaeI     EciI  Eco57MI
    EcoNI EcoO109I     FalI    HaeII    Hin4I   HincII     KasI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI
    PflMI     PfoI     PmlI    PpuMI    PshAI   PspOMI     PstI     PvuI
    PvuII    RsrII    SacII    SanDI     SfcI     SfoI     SmaI     SmlI
     SphI     StyI    TaqII     TstI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI
     AleI     AloI    AlwNI     ApoI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BcgI    BciVI     BclI    BfrBI     BlpI    BmgBI
     BmtI     BsgI    BsiWI     BsmI    BspEI    BsrGI   BssHII    BssSI
   BstAPI    BstBI  BstZ17I   Bsu36I    BtgZI     ClaI     DraI     EagI
     EarI  EcoICRI    EcoRI    EcoRV     FseI     FspI    FspAI  HindIII
     HpaI     KpnI     MfeI     MluI     NdeI     NheI     NotI     NruI
     NsiI     PacI     PasI     PciI     PmeI     PpiI     PsiI    PspXI
     PsrI     SacI     SalI     SapI     SbfI     ScaI    SexAI     SfiI
     SgfI    SgrAI    SnaBI     SpeI     SrfI     SspI     StuI     SwaI
     TatI  Tth111I     XbaI     XhoI     XmnI     ZraI
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