Restriction map for pF1KB9743
##################################
# NO CUT ENZYMES (  125 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AflIII      AhdI        Alw44I      
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AvrII       BamHI       
BbeI        BbrPI       BbsI        BciVI       BclI        
BfaI        BfrI        BglII       BlnI        BlpI        
Bpu1102I    BpuAI       BseAI       BsiWI       BsmBI       
BsmI        Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrDI       BsrGI       BssHII      Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstZ17I     
CelII       ClaI        CpoI        DraI        DrdI        
EagI        Eam1105I    EarI        EclXI       Eco52I      
EcoNI       EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        FseI        HindIII     HpaI        KasI        
KpnI        Ksp632I     KspI        MaeI        MfeI        
MluI        MroI        MunI        NaeI        NarI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacII       
SalI        SapI        SbfI        ScaI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        TfiI        Tsp509I     
XbaI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

AccII         851  CGCG                 CG^CG
AgeI          761  ACCGGT               A^CCGGT
AlwI          753  GATCC                GGATC(4/5)
AlwNI         352  CAGCCACTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          167  CCTCAGG              CC^T[ACGT]AGG
AspI          559  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII         929  TGCGCA               TGC^GCA
BbvCI         240  CCTCAGC              CCTCAGC(-5/-2)
BcgI          588  CGAGAAGGATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI         1097  GCCTGCCCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI         862  TACGTG               [CT]AC^GT[GA]
BsaI         1290  GGTCTC               GGTCTC(1/5)
BsaWI         761  ACCGGT               [AT]^CCGG[AT]
BseRI         438  CTCCTC               GAGGAG(10/8)
BsiEI         763  CGGTCG               CG[GA][CT]^CG
BsmAI        1291  GTCTC                GTCTC(1/5)
BsrBI          19  CCGCTC               CCGCTC(-3/-3)
BstUI         851  CGCG                 CG^CG
BstYI         752  AGATCC               [GA]^GATC[CT]
Bsu36I        167  CCTCAGG              CC^T[ACGT]AGG
Eco81I        167  CCTCAGG              CC^T[ACGT]AGG
FspI          929  TGCGCA               TGC^GCA
MflI          752  AGATCC               [GA]^GATC[CT]
MseI          842  TTAA                 T^TAA
MslI          769  CACACAGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI          851  CGCG                 CG^CG
PflFI         559  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI         761  ACCGGT               A^CCGGT
PpuMI         317  GGGACCT              [GA]G^G[AT]CC[CT]
SexAI         551  ACCTGGT              A^CC[AT]GGT
TaqI           23  TCGA                 T^CGA
Tru9I         842  TTAA                 T^TAA
Tth111I       559  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I        23  TCGA                 T^CGA
XcmI         1355  CCACCAGGGCCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         752  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   36 enzymes) #
###################################

AcyI         1386  GACGCC               G[GA]^CG[CT]C
             1477  GGCGTC              
AfaI          603  GTAC                 GT^AC
              657  GTAC                
Aor51HI       815  AGCGCT               AGC^GCT
              905  AGCGCT              
BalI          951  TGGCCA               TGG^CCA
             1232  TGGCCA              
BanI         1350  GGTGCC               G^G[CT][GA]CC
             1453  GGCACC              
BsaBI         663  GATGCTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1056  GATCATCATC          
BsaHI        1386  GACGCC               G[GA]^CG[CT]C
             1477  GGCGTC              
BsgI          106  CTGCAC               GTGCAG(16/14)
              424  CTGCAC              
BsrFI         761  ACCGGT               [GA]^CCGG[CT]
             1557  ACCGGC              
BssSI         967  CACGAG               CACGAG(-5/-1)
              232  CTCGTG              
BstXI         494  CCAAGCAGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1358  CCAGGGCCCTGG        
Cfr10I        761  ACCGGT               [GA]^CCGG[CT]
             1557  ACCGGC              
DpnII         753  GATC                 ^GATC
             1056  GATC                
DraIII        859  CACTACGTG            CAC[ACGT][ACGT][ACGT]^GTG
              955  CACTTTGTG           
Eco47III      815  AGCGCT               AGC^GCT
              905  AGCGCT              
Hin1I        1386  GACGCC               G[GA]^CG[CT]C
             1477  GGCGTC              
HincII        565  GTCAAC               GT[CT]^[GA]AC
             1480  GTCAAC              
HindII        565  GTCAAC               GT[CT]^[GA]AC
             1480  GTCAAC              
HinfI         268  GACTC                G^A[ACGT]TC
             1436  GACTC               
MaeII         788  ACGT                 A^CGT
              863  ACGT                
MamI          663  GATGCTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1056  GATCATCATC          
MboI          753  GATC                 ^GATC
             1056  GATC                
MboII         750  GAAGA                GAAGA(8/7)
              395  TCTTC               
MluNI         951  TGGCCA               TGG^CCA
             1232  TGGCCA              
MscI          951  TGGCCA               TGG^CCA
             1232  TGGCCA              
NcoI          633  CCATGG               C^CATGG
              948  CCATGG              
NdeII         753  GATC                 ^GATC
             1056  GATC                
PflMI         253  CCACCATCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              858  CCACTACGTGG         
PleI          268  GACTC                GAGTC(4/5)
             1436  GACTC               
PstI          717  CTGCAG               CTGCA^G
              985  CTGCAG              
RsaI          603  GTAC                 GT^AC
              657  GTAC                
SacI          538  GAGCTC               GAGCT^C
              979  GAGCTC              
Sau3AI        753  GATC                 ^GATC
             1056  GATC                
SfcI          717  CTGCAG               C^T[GA][CT]AG
              985  CTGCAG              
Tsp45I        249  GTCAC                ^GT[GC]AC
              833  GTGAC               
Van91I        253  CCACCATCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              858  CCACTACGTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   61 enzymes) #
#########################################

AciI        AluI        ApaI        AspHI       AvaI        
AvaII       BanII       BbvI        BcnI        BmyI        
BpmI        BsaJI       BsiHKAI     BsiYI       BslI        
BsmFI       BsoBI       Bsp1286I    BspMI       BsrI        
BssKI       BstF5I      BstNI       Cac8I       CfoI        
Cfr13I      DdeI        DraII       DsaI        EaeI        
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        HinP1I      HpaII       HphI        ItaI        
MaeIII      MnlI        MspA1I      MspI        MvaI        
MwoI        NciI        NlaIII      NlaIV       PvuII       
Sau96I      ScrFI       SfaNI       StyI        TseI        
TspRI       
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