Restriction map for pF1KB9707
 (Linear) (Six-base) MAPSORT of: KIBB9707.seq  Check: 969  from: 1  to: 1026



KIBB9707.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:11  ..

Acc65I G'GTAC_C

Cuts at:      0     623    1026 
   Size:        623     403
 
AflIII A'CryG_T

Cuts at:      0     947    1026 
   Size:        947      79
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     159    1026 
   Size:        159     867
 
AlwNI CAG_nnn'CTG

Cuts at:      0     324    1026 
   Size:        324     702
 
ApaI G_GGCC'C

Cuts at:      0     331     352     479     554     562    1026 
   Size:        331      21     127      75       8     464

  Fragments arranged by size: 

                 464     331     127      75      21       8
 
ApaLI G'TGCA_C

Cuts at:      0     285    1026 
   Size:        285     741
 
AvaI C'yCGr_G

Cuts at:      0     345     585    1026 
   Size:        345     240     441

  Fragments arranged by size: 

                 441     345     240
 
BanI G'GyrC_C

Cuts at:      0     310     376     604     623    1026 
   Size:        310      66     228      19     403

  Fragments arranged by size: 

                 403     310     228      66      19
 
BanII G_rGCy'C

Cuts at:      0     199     239     331     352     479     554     562     634 
   Size:        199      40      92      21     127      75       8      72

Cuts at:    634     664    1026 
   Size:         30     362

  Fragments arranged by size: 

                 362     199     127      92      75      72      40      30
                  21       8
 
BbeI G_GCGC'C

Cuts at:      0     314     380    1026 
   Size:        314      66     646

  Fragments arranged by size: 

                 646     314      66
 
BglI GCCn_nnn'nGGC

Cuts at:      0     685    1026 
   Size:        685     341
 
BlpI GC'TnA_GC

Cuts at:      0     633     883    1026 
   Size:        633     250     143

  Fragments arranged by size: 

                 633     250     143
 
Bme1580I G_kGCm'C

Cuts at:      0     289     331     352     479     554     562     974    1026 
   Size:        289      42      21     127      75       8     412      52

  Fragments arranged by size: 

                 412     289     127      75      52      42      21       8
            
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      24     201     504    1026 
   Size:         24     177     303     522

  Fragments arranged by size: 

                 522     303     177      24
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     258     435     653    1026 
   Size:        258     177     218     373

  Fragments arranged by size: 

                 373     258     218     177
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      29     763    1026 
   Size:         29     734     263

  Fragments arranged by size: 

                 734     263      29
 
BsaAI yAC'GTr

Cuts at:      0     950    1026 
   Size:        950      76
 
BsaHI Gr'CG_yC

Cuts at:      0     311     377    1026 
   Size:        311      66     649

  Fragments arranged by size: 

                 649     311      66
 
BsaWI w'CCGG_w

Cuts at:      0     987    1026 
   Size:        987      39
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     181     211    1026 
   Size:        181      30     815

  Fragments arranged by size: 

                 815     181      30
 
BseYI C'CCAG_C

Cuts at:      0     458     973    1026 
   Size:        458     515      53

  Fragments arranged by size: 

                 515     458      53
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     193    1026 
   Size:        193     833
 
BsiHKAI G_wGCw'C

Cuts at:      0     289     634    1026 
   Size:        289     345     392

  Fragments arranged by size: 

                 392     345     289
 
Bsp1286I G_dGCh'C

Cuts at:      0     199     239     289     331     352     479     554     562 
   Size:        199      40      50      42      21     127      75       8

Cuts at:    562     634     664     974    1026 
   Size:         72      30     310      52

  Fragments arranged by size: 

                 310     199     127      75      72      52      50      42
                  40      30      21       8
 
BspEI T'CCGG_A

Cuts at:      0     987    1026 
   Size:        987      39
 
BsrBI CCG'CTC

Cuts at:      0     308     932    1026 
   Size:        308     624      94

  Fragments arranged by size: 

                 624     308      94
 
BsrFI r'CCGG_y

Cuts at:      0     364    1026 
   Size:        364     662
 
BssHII G'CGCG_C

Cuts at:      0     429    1026 
   Size:        429     597
 
BssSI C'ACGA_G

Cuts at:      0     420    1026 
   Size:        420     606
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     828    1026 
   Size:        828     198
 
BtgI C'CryG_G

Cuts at:      0     223     256     380     401     554    1026 
   Size:        223      33     124      21     153     472

  Fragments arranged by size: 

                 472     223     153     124      33      21
 
BtsI GCAGTG_nn'

Cuts at:      0     507    1026 
   Size:        507     519
 
DraIII CAC_nnn'GTG

Cuts at:      0     422    1026 
   Size:        422     604
 
EarI CTCTTCn'nnn_

Cuts at:      0     257    1026 
   Size:        257     769
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     247    1019    1026 
   Size:        247     772       7

  Fragments arranged by size: 

                 772     247       7
 
EcoICRI GAG'CTC

Cuts at:      0     632    1026 
   Size:        632     394
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      29     763    1026 
   Size:         29     734     263

  Fragments arranged by size: 

                 734     263      29
 
EcoO109I rG'GnC_Cy

Cuts at:      0     328     349     475     715     891    1026 
   Size:        328      21     126     240     176     135

  Fragments arranged by size: 

                 328     240     176     135     126      21
 
FspI TGC'GCA

Cuts at:      0     925    1026 
   Size:        925     101
 
HaeII r_GCGC'y

Cuts at:      0     314     380    1026 
   Size:        314      66     646

  Fragments arranged by size: 

                 646     314      66
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      10      42     181     213    1026 
   Size:         10      32     139      32     813

  Fragments arranged by size: 

                 813     139      32      32      10
 
HindIII A'AGCT_T

Cuts at:      0     174    1026 
   Size:        174     852
 
KasI G'GCGC_C

Cuts at:      0     310     376    1026 
   Size:        310      66     650

  Fragments arranged by size: 

                 650     310      66
 
KpnI G_GTAC'C

Cuts at:      0     627    1026 
   Size:        627     399
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     650     763    1026 
   Size:        650     113     263

  Fragments arranged by size: 

                 650     263     113
 
MslI CAynn'nnrTG

Cuts at:      0     599    1026 
   Size:        599     427
 
MspA1I CmG'CkG

Cuts at:      0     186     382     458     556     726     773     810    1026 
   Size:        186     196      76      98     170      47      37     216

  Fragments arranged by size: 

                 216     196     186     170      98      76      47      37
            
 
NaeI GCC'GGC

Cuts at:      0     366    1026 
   Size:        366     660
 
NarI GG'CG_CC

Cuts at:      0     311     377    1026 
   Size:        311      66     649

  Fragments arranged by size: 

                 649     311      66
 
NcoI C'CATG_G

Cuts at:      0     223    1026 
   Size:        223     803
 
NgoMIV G'CCGG_C

Cuts at:      0     364    1026 
   Size:        364     662
 
NspI r_CATG'y

Cuts at:      0     146     598    1026 
   Size:        146     452     428

  Fragments arranged by size: 

                 452     428     146
 
PasI CC'CwG_GG

Cuts at:      0     324    1026 
   Size:        324     702
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     422     542     578    1026 
   Size:        422     120      36     448

  Fragments arranged by size: 

                 448     422     120      36
 
PfoI T'CCnGG_A

Cuts at:      0     577     740    1026 
   Size:        577     163     286

  Fragments arranged by size: 

                 577     286     163
 
PpuMI rG'GwC_Cy

Cuts at:      0     715    1026 
   Size:        715     311
 
PspOMI G'GGCC_C

Cuts at:      0     327     348     475     550     558    1026 
   Size:        327      21     127      75       8     468

  Fragments arranged by size: 

                 468     327     127      75      21       8
 
PvuII CAG'CTG

Cuts at:      0     186     458     726     773     810    1026 
   Size:        186     272     268      47      37     216

  Fragments arranged by size: 

                 272     268     216     186      47      37
 
RsrII CG'GwC_CG

Cuts at:      0     672    1026 
   Size:        672     354
 
SacI G_AGCT'C

Cuts at:      0     634    1026 
   Size:        634     392
 
SacII CC_GC'GG

Cuts at:      0     383     557    1026 
   Size:        383     174     469

  Fragments arranged by size: 

                 469     383     174
 
SapI GCTCTTCn'nnn_

Cuts at:      0     257    1026 
   Size:        257     769
 
SfoI GGC'GCC

Cuts at:      0     312     378    1026 
   Size:        312      66     648

  Fragments arranged by size: 

                 648     312      66
 
SmaI CCC'GGG

Cuts at:      0     347     587    1026 
   Size:        347     240     439

  Fragments arranged by size: 

                 439     347     240
 
SphI G_CATG'C

Cuts at:      0     598    1026 
   Size:        598     428
 
SrfI GCCC'GGGC

Cuts at:      0     347    1026 
   Size:        347     679
 
StyI C'CwwG_G

Cuts at:      0     111     223    1026 
   Size:        111     112     803

  Fragments arranged by size: 

                 803     112     111
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     227    1026 
   Size:        227     799
 
TatI w'GTAC_w

Cuts at:      0     944    1026 
   Size:        944      82
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     188     221    1026 
   Size:        188      33     805

  Fragments arranged by size: 

                 805     188      33
 
XmaI C'CCGG_G

Cuts at:      0     345     585    1026 
   Size:        345     240     441

  Fragments arranged by size: 

                 441     345     240
 

 Enzymes that do cut:

   Acc65I   AflIII     AlfI    AlwNI     ApaI    ApaLI     AvaI     BanI
    BanII     BbeI     BglI     BlpI Bme1580I     BmrI     BplI     BpmI
    BsaAI    BsaHI    BsaWI    BsaXI    BseYI     BsgI  BsiHKAI Bsp1286I
    BspEI    BsrBI    BsrFI   BssHII    BssSI    BstXI     BtgI     BtsI
   DraIII     EarI     EciI  EcoICRI  Eco57MI EcoO109I     FspI    HaeII
    Hin4I  HindIII     KasI     KpnI     MmeI     MslI   MspA1I     NaeI
     NarI     NcoI   NgoMIV     NspI     PasI    PflMI     PfoI    PpuMI
   PspOMI    PvuII    RsrII     SacI    SacII     SapI     SfoI     SmaI
     SphI     SrfI     StyI    TaqII     TatI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AcuI     AfeI    AflII     AgeI
     AhdI     AleI     AloI     ApoI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
    BglII    BmgBI     BmtI   Bpu10I    BpuEI     BsaI    BsaBI    BseRI
    BsiEI    BsiWI     BsmI    BsmBI    BspHI    BspMI    BsrDI    BsrGI
   BstAPI    BstBI   BstEII    BstYI  BstZ17I   Bsu36I    BtgZI     ClaI
    CspCI     DraI     DrdI     EaeI     EagI    EcoNI    EcoRI    EcoRV
     FalI     FseI    FspAI   HincII     HpaI     MfeI     MluI     MscI
     NdeI     NheI     NotI     NruI     NsiI     PacI     PciI     PmeI
     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI     PstI     PvuI
     SalI    SanDI     SbfI     ScaI    SexAI     SfcI     SfiI     SgfI
    SgrAI     SmlI    SnaBI     SpeI     SspI     StuI     SwaI     TstI
  Tth111I     XbaI     XhoI     XmnI     ZraI
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