Restriction map for pF1KB9685
##################################
# NO CUT ENZYMES (  194 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AflII       AflIII      
AgeI        Alw44I      AlwI        AlwNI       AocI        
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp700I     Asp718I     AspHI       AspI        
AvaI        AviII       AvrII       BamHI       BanI        
BanII       BbeI        BbrPI       BbvCI       BbvI        
BcgI        BciVI       BcnI        BfaI        BfrI        
BglI        BglII       BlnI        BlpI        BmyI        
Bpu1102I    BsaAI       BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BsiEI       BsiHKAI     BsiWI       
BsiYI       BslI        BsmBI       BsmI        BsoBI       
Bsp1286I    Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstXI       BstYI       
BstZ17I     Bsu36I      Cac8I       CelII       CfoI        
Cfr10I      ClaI        CpoI        DraI        DraII       
DraIII      DrdI        DsaI        EagI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRV       EcoT22I     FauI        Fnu4HI      
FseI        FspI        HaeII       HapII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HpaI        HpaII       ItaI        KasI        KpnI        
KspI        MaeI        MamI        MfeI        MflI        
MluI        MroI        MslI        MspA1I      MspI        
MunI        MvnI        MwoI        NaeI        NarI        
NciI        NcoI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshBI       
Psp1406I    PstI        PvuI        PvuII       RcaI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SfaNI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        TaqI        TseI        Tth111I     
TthHB8I     Van91I      XbaI        XcmI        XhoI        
XhoII       XmaCI       XmaI        XmnI        


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# ONE CUT ENZYMES (   43 enzymes) #
###################################

AfaI          373  GTAC                 GT^AC
AhdI          113  GACACCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI         113  GACACCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII           4  GGACC                G^G[AT]CC
BalI           26  TGGCCA               TGG^CCA
BbsI          121  GTCTTC               GAAGAC(2/6)
BclI          155  TGATCA               T^GATCA
BpuAI         121  GTCTTC               GAAGAC(2/6)
BsaJI         126  CCTTGG               C^C[ACGT][ACGT]GG
BseRI          46  GAGGAG               GAGGAG(10/8)
BsgI           68  CTGCAC               GTGCAG(16/14)
BsmFI           3  GGGAC                GGGAC(10/14)
BsrDI          55  CATTGC               GCAATG(2/0)
Cfr13I          4  GGACC                G^G[ACGT]CC
DdeI          143  CTAAG                C^T[ACGT]AG
DpnII         156  GATC                 ^GATC
EaeI           26  TGGCCA               [CT]^GGCC[GA]
Eam1105I      113  GACACCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          195  GAAGAG               CTCTTC(1/4)
EcoRI          34  GAATTC               G^AATTC
EcoT14I       126  CCTTGG               C^C[AT][AT]GG
FbaI          155  TGATCA               T^GATCA
HaeIII         27  GGCC                 GG^CC
HindIII       539  AAGCTT               A^AGCTT
HinfI         277  GATTC                G^A[ACGT]TC
HphI          158  TCACC                GGTGA(8/7)
Ksp632I       195  GAAGAG               CTCTTC(1/4)
MaeII         315  ACGT                 A^CGT
MboI          156  GATC                 ^GATC
MluNI          26  TGGCCA               TGG^CCA
MscI           26  TGGCCA               TGG^CCA
NdeI          446  CATATG               CA^TATG
NdeII         156  GATC                 ^GATC
NlaIII        202  CATG                 CATG^
NlaIV           3  GGGACC               GG[ACGT]^[ACGT]CC
PshAI         250  GACCTTTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsaI          373  GTAC                 GT^AC
Sau3AI        156  GATC                 ^GATC
Sau96I          4  GGACC                G^G[ACGT]CC
SexAI         116  ACCTGGT              A^CC[AT]GGT
SfcI          108  CTACAG               C^T[GA][CT]AG
StyI          126  CCTTGG               C^C[AT][AT]GG
TfiI          277  GATTC                G^A[AT]TC


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

AluI          414  AGCT                 AG^CT
              540  AGCT                
BpmI           43  CTGGAG               CTGGAG(16/14)
              166  CTGGAG              
BsmAI         198  GAGAC                GTCTC(1/5)
              457  GTCTC               
BssKI          60  CCTGG                ^CC[ACGT]GG
              117  CCTGG               
BstF5I         90  GGATG                GGATG(2/0)
              360  GGATG               
BstNI          60  CCTGG                CC^[AT]GG
              117  CCTGG               
EcoRII         60  CCTGG                ^CC[AT]GG
              117  CCTGG               
FokI           90  GGATG                GGATG(9/13)
              360  GGATG               
MaeIII        139  GTCAC                ^GT[ACGT]AC
              298  GTGAC               
MboII         195  GAAGA                GAAGA(8/7)
              122  TCTTC               
MseI          337  TTAA                 T^TAA
              398  TTAA                
MvaI           60  CCTGG                CC^[AT]GG
              117  CCTGG               
ScrFI          60  CCTGG                CC^[ACGT]GG
              117  CCTGG               
Tru9I         337  TTAA                 T^TAA
              398  TTAA                
Tsp45I        139  GTCAC                ^GT[GC]AC
              298  GTGAC               
TspRI          66  CACTG                CA[GC]TG[ACGT][ACGT]^
              189  CACTG               


#########################################
# 3 OR MORE CUT ENZYMES (    5 enzymes) #
#########################################

AcsI        ApoI        BsrI        MnlI        Tsp509I     
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