Restriction map for pF1KB9637
 (Circular) (Six-base) MAPSORT of: pF1KB9637.seq  Check: 4199  from: 1  to: 4346



pF1KB9637  4346 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:31  ..

AccI GT'mk_AC

Cuts at:   1384    1384 
   Size:       4346
 
Acc65I G'GTAC_C

Cuts at:   1362    1362 
   Size:       4346
 
AclI AA'CG_TT

Cuts at:   4186    4186 
   Size:       4346
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    673    2171    2603    3350    3618     673 
   Size:       1498     432     747     268    1401

  Fragments arranged by size: 

                1498    1401     747     432     268
 
AflIII A'CryG_T

Cuts at:   1698    2802    3779    1698 
   Size:       1104     977    2265

  Fragments arranged by size: 

                2265    1104     977
 
AgeI A'CCGG_T

Cuts at:   2734    2734 
   Size:       4346
 
AleI CACnn'nnGTG

Cuts at:    140     349     140 
   Size:        209    4137
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    367     367 
   Size:       4346
 
AlwNI CAG_nnn'CTG

Cuts at:    582     783    1057    1651    3218     582 
   Size:        201     274     594    1567    1710

  Fragments arranged by size: 

                1710    1567     594     274     201
 
ApaLI G'TGCA_C

Cuts at:   3116    3116 
   Size:       4346
 
ApoI r'AATT_y

Cuts at:   1350    3699    3955    1350 
   Size:       2349     256    1741

  Fragments arranged by size: 

                2349    1741     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4346
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4346
 
AvaI C'yCGr_G

Cuts at:   1366    1366 
   Size:       4346
 
BamHI G'GATC_C

Cuts at:   1371    1371 
   Size:       4346
 
BanI G'GyrC_C

Cuts at:    275    1362    1776    2024    2059     275 
   Size:       1087     414     248      35    2562

  Fragments arranged by size: 

                2562    1087     414     248      35
 
BanII G_rGCy'C

Cuts at:   1360    2390    1360 
   Size:       1030    3316
 
BbeI G_GCGC'C

Cuts at:   1780    2028    1780 
   Size:        248    4098
 
BbsI GAAGACnn'nnnn_

Cuts at:    163     571     163 
   Size:        408    3938
 
BbvCI CC'TCA_GC

Cuts at:    725     725 
   Size:       4346
 
BciVI GTATCCnnnnn_n'

Cuts at:    828    2244    3005    4346     828 
   Size:       1416     761    1341     828

  Fragments arranged by size: 

                1416    1341     828     761
 
BclI T'GATC_A

Cuts at:   1866    1866 
   Size:       4346
 
BfrBI ATG'CAT

Cuts at:   1333    1333 
   Size:       4346
 
BglII A'GATC_T

Cuts at:   1861    3898    1861 
   Size:       2037    2309
 
BlpI GC'TnA_GC

Cuts at:   1458    1458 
   Size:       4346
 
Bme1580I G_kGCm'C

Cuts at:    330     522    1243    1971    2064    3120     330 
   Size:        192     721     728      93    1056    1556

  Fragments arranged by size: 

                1556    1056     728     721     192      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1558    1639    1974    4118    1558 
   Size:         81     335    2144    1786

  Fragments arranged by size: 

                2144    1786     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2713    2713 
   Size:       4346
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    178     352     178 
   Size:        174    4172
 
Bpu10I CC'TnA_GC

Cuts at:    725    1007     725 
   Size:        282    4064
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1522    2401    2893    3191    1522 
   Size:        879     492     298    2677

  Fragments arranged by size: 

                2677     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    602    4050     602 
   Size:       3448     898
 
BsaAI yAC'GTr

Cuts at:   1699    1699 
   Size:       4346
 
BsaBI GATnn'nnATC

Cuts at:      6    1860    3897       6 
   Size:       1854    2037     455

  Fragments arranged by size: 

                2037    1854     455
 
BsaHI Gr'CG_yC

Cuts at:    468    1777    2025    2727    4223     468 
   Size:       1309     248     702    1496     591

  Fragments arranged by size: 

                1496    1309     702     591     248
 
BsaWI w'CCGG_w

Cuts at:    167     604    1535    2056    2734    3008    3155    3566     167 
   Size:        437     931     521     678     274     147     411     947

  Fragments arranged by size: 

                 947     931     678     521     437     411     274     147
            
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    105     135     105 
   Size:         30    4316
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    247     821     247 
   Size:        574    3772
 
BseYI C'CCAG_C

Cuts at:    261     767    1771    2529    3106    3602     261 
   Size:        506    1004     758     577     496    1005

  Fragments arranged by size: 

                1005    1004     758     577     506     496
 
BsiEI CG_ry'CG

Cuts at:    104    1888    1934    3142     104 
   Size:       1784      46    1208    1308

  Fragments arranged by size: 

                1784    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    147     346     622    1360    2138    2328    3120     147 
   Size:        199     276     738     778     190     792    1373

  Fragments arranged by size: 

                1373     792     778     738     276     199     190
 
BsmI GAATG_Cn'

Cuts at:   1333    1333 
   Size:       4346
 
Bsp1286I G_dGCh'C

Cuts at:    147     330     346     522     622    1243    1360    1971    2064 
   Size:        183      16     176     100     621     117     611      93

Cuts at:   2064    2138    2328    2390    3120     147 
   Size:         74     190      62     730    1373

  Fragments arranged by size: 

                1373     730     621     611     190     183     176     117
                 100      93      74      62      16
 
BspEI T'CCGG_A

Cuts at:   3566    3566 
   Size:       4346
 
BspHI T'CATG_A

Cuts at:   3522    3522 
   Size:       4346
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1040    1083    1396    1610    1912    2293    1040 
   Size:         43     313     214     302     381    3093

  Fragments arranged by size: 

                3093     381     313     302     214      43
 
BsrBI CCG'CTC

Cuts at:     50    2638    2692    4176    4341      50 
   Size:       2588      54    1484     165      55

  Fragments arranged by size: 

                2588    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2258    2258 
   Size:       4346
 
BsrFI r'CCGG_y

Cuts at:   2344    2525    2734    2344 
   Size:        181     209    3956

  Fragments arranged by size: 

                3956     209     181
 
BssSI C'ACGA_G

Cuts at:   2617    2975    2617 
   Size:        358    3988
 
BstAPI GCAn_nnn'nTGC

Cuts at:    783    1723     783 
   Size:        940    3406
 
BstBI TT'CG_AA

Cuts at:   2707    2707 
   Size:       4346
 
BstXI CCAn_nnnn'nTGG

Cuts at:    216     529     216 
   Size:        313    4033
 
BstYI r'GATC_y

Cuts at:      1    1371    1838    1861    2196    2442    3454    3540    3552 
   Size:       1370     467      23     335     246    1012      86      12

Cuts at:   3552    3898       1 
   Size:        346     449

  Fragments arranged by size: 

                1370    1012     467     449     346     335     246      86
                  23      12
 
BtgI C'CryG_G

Cuts at:    107    1235     107 
   Size:       1128    3218
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2296    2477    2640    2296 
   Size:        181     163    4002

  Fragments arranged by size: 

                4002     181     163
 
BtsI GCAGTG_nn'

Cuts at:    306    1589     306 
   Size:       1283    3063
 
DraI TTT'AAA

Cuts at:   1345    1345 
   Size:       4346
 
DraIII CAC_nnn'GTG

Cuts at:    477     477 
   Size:       4346
 
DrdI GACnn_nn'nnGTC

Cuts at:   2052    2910    2052 
   Size:        858    3488
 
EaeI y'GGCC_r

Cuts at:    293    1931    2105    2496    2523    3868     293 
   Size:       1638     174     391      27    1345     771

  Fragments arranged by size: 

                1638    1345     771     391     174      27
 
EagI C'GGCC_G

Cuts at:   1931    1931 
   Size:       4346
 
EarI CTCTTCn'nnn_

Cuts at:    377    2369    2579     377 
   Size:       1992     210    2144

  Fragments arranged by size: 

                2144    1992     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    824    2554    2864    3010    4156     824 
   Size:       1730     310     146    1146    1014

  Fragments arranged by size: 

                1730    1146    1014     310     146
 
EcoICRI GAG'CTC

Cuts at:   1358    1358 
   Size:       4346
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    178     352     673    2171    2603    3350    3618     178 
   Size:        174     321    1498     432     747     268     906

  Fragments arranged by size: 

                1498     906     747     432     321     268     174
 
EcoNI CCTnn'n_nnAGG

Cuts at:   1036    1036 
   Size:       4346
 
EcoO109I rG'GnC_Cy

Cuts at:    306    1168    1485     306 
   Size:        862     317    3167

  Fragments arranged by size: 

                3167     862     317
 
EcoRI G'AATT_C

Cuts at:   1350    1350 
   Size:       4346
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2365    2365 
   Size:       4346
 
FspI TGC'GCA

Cuts at:   2127    2127 
   Size:       4346
 
HaeII r_GCGC'y

Cuts at:   1780    2028    3050    4029    1780 
   Size:        248    1022     979    2097

  Fragments arranged by size: 

                2097    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    955     987    2188    2218    2220    2250     955 
   Size:         32    1201      30       2      30    3051

  Fragments arranged by size: 

                3051    1201      32      30      30       2
 
HincII GTy'rAC

Cuts at:   1385    1385 
   Size:       4346
 
KasI G'GCGC_C

Cuts at:   1776    2024    1776 
   Size:        248    4098
 
KpnI G_GTAC'C

Cuts at:   1366    1366 
   Size:       4346
 
MluI A'CGCG_T

Cuts at:   3779    3779 
   Size:       4346
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    672    3017    3201     672 
   Size:       2345     184    1817

  Fragments arranged by size: 

                2345    1817     184
 
MscI TGG'CCA

Cuts at:   2107    2107 
   Size:       4346
 
MslI CAynn'nnrTG

Cuts at:    140     349    2335    2462     140 
   Size:        209    1986     127    2024

  Fragments arranged by size: 

                2024    1986     209     127
 
MspA1I CmG'CkG

Cuts at:    724     783    1116    1294    1457    1771    2131    3144    3389 
   Size:         59     333     178     163     314     360    1013     245

Cuts at:   3389     724 
   Size:       1681

  Fragments arranged by size: 

                1681    1013     360     333     314     245     178     163
                  59
 
NaeI GCC'GGC

Cuts at:   2527    2527 
   Size:       4346
 
NarI GG'CG_CC

Cuts at:   1777    2025    1777 
   Size:        248    4098
 
NcoI C'CATG_G

Cuts at:    107    1235     107 
   Size:       1128    3218
 
NgoMIV G'CCGG_C

Cuts at:   2525    2525 
   Size:       4346
 
NsiI A_TGCA'T

Cuts at:   1335    1335 
   Size:       4346
 
NspI r_CATG'y

Cuts at:   1399    1899    2806    1399 
   Size:        500     907    2939

  Fragments arranged by size: 

                2939     907     500
 
PasI CC'CwG_GG

Cuts at:   1164    1164 
   Size:       4346
 
PciI A'CATG_T

Cuts at:   2802    2802 
   Size:       4346
 
PflMI CCAn_nnn'nTGG

Cuts at:    477     477 
   Size:       4346
 
PmeI GTTT'AAAC

Cuts at:   1345    1345 
   Size:       4346
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    145     177    3485    3517     145 
   Size:         32    3308      32     974

  Fragments arranged by size: 

                3308     974      32      32
 
PpuMI rG'GwC_Cy

Cuts at:    306     306 
   Size:       4346
 
PstI C_TGCA'G

Cuts at:   1053    1393    2078    1053 
   Size:        340     685    3321

  Fragments arranged by size: 

                3321     685     340
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4346
 
PvuII CAG'CTG

Cuts at:    724    1116    1294    1771    2131     724 
   Size:        392     178     477     360    2939

  Fragments arranged by size: 

                2939     477     392     360     178
 
RsrII CG'GwC_CG

Cuts at:   2541    2541 
   Size:       4346
 
SacI G_AGCT'C

Cuts at:   1360    1360 
   Size:       4346
 
SalI G'TCGA_C

Cuts at:   1383    1383 
   Size:       4346
 
SapI GCTCTTCn'nnn_

Cuts at:   2369    2579    2369 
   Size:        210    4136
 
SbfI CC_TGCA'GG

Cuts at:   1393    1393 
   Size:       4346
 
ScaI AGT'ACT

Cuts at:   3863    3863 
   Size:       4346
 
SexAI A'CCwGG_T

Cuts at:    473     473 
   Size:       4346
 
SfcI C'TryA_G

Cuts at:     33    1049    1389    2074    3067    3258      33 
   Size:       1016     340     685     993     191    1121

  Fragments arranged by size: 

                1121    1016     993     685     340     191
 
SfoI GGC'GCC

Cuts at:   1778    2026    1778 
   Size:        248    4098
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4346
 
SmaI CCC'GGG

Cuts at:   1368    1368 
   Size:       4346
 
SmlI C'TyrA_G

Cuts at:   1501    2416    2908    3170    1501 
   Size:        915     492     262    2677

  Fragments arranged by size: 

                2677     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4346
 
SphI G_CATG'C

Cuts at:   1399    1899    1399 
   Size:        500    3846
 
StyI C'CwwG_G

Cuts at:    107     404    1037    1235    1480    1834     107 
   Size:        297     633     198     245     354    2619

  Fragments arranged by size: 

                2619     633     354     297     245     198
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    215    1929    2062    2521    2730     215 
   Size:       1714     133     459     209    1831

  Fragments arranged by size: 

                1831    1714     459     209     133
 
TatI w'GTAC_w

Cuts at:    248     857    3861     248 
   Size:        609    3004     733

  Fragments arranged by size: 

                3004     733     609
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    490     522     732     764    3863    3895     490 
   Size:         32     210      32    3099      32     941

  Fragments arranged by size: 

                3099     941     210      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   2143    2143 
   Size:       4346
 
XbaI T'CTAG_A

Cuts at:   1377    1377 
   Size:       4346
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1141    1841    1141 
   Size:        700    3646
 
XmaI C'CCGG_G

Cuts at:   1366    1366 
   Size:       4346
 
XmnI GAAnn'nnTTC

Cuts at:    160     160 
   Size:       4346
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AleI     AlfI
    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI    BciVI     BclI    BfrBI    BglII
     BlpI Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI    BsiEI
  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BssSI   BstAPI    BstBI    BstXI    BstYI     BtgI    BtgZI
     BtsI     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FspI    HaeII
    Hin4I   HincII     KasI     KpnI     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI   NgoMIV     NsiI     NspI     PasI
     PciI    PflMI     PmeI     PpiI    PpuMI     PstI     PvuI    PvuII
    RsrII     SacI     SalI     SapI     SbfI     ScaI    SexAI     SfcI
     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AloI     ApaI     AscI
    AvrII     BaeI     BcgI     BglI    BmgBI     BmtI     BsgI    BsiWI
    BsmBI    BsrGI   BssHII   BstEII  BstZ17I   Bsu36I     ClaI    CspCI
    EcoRV     FseI    FspAI  HindIII     HpaI     MfeI     NdeI     NheI
     NotI     NruI     PacI     PfoI     PmlI    PshAI     PsiI   PspOMI
    PspXI     PsrI    SacII    SanDI     SfiI    SgrAI    SnaBI     SrfI
     SspI     StuI     SwaI     XhoI     ZraI
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