Restriction map for pF1KB9580
##################################
# NO CUT ENZYMES (  168 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        AlwI        AlwNI       AocI        Aor51HI     
ApaI        ApoI        AscI        AseI        AsnI        
Asp718I     AspEI       AvaI        AviII       AvrII       
BalI        BamHI       BanI        BbeI        BbsI        
BbvCI       BcgI        BciVI       BclI        BfrI        
BglI        BglII       BlnI        BlpI        Bpu1102I    
BpuAI       BsaBI       BsaHI       BsaWI       BseAI       
BsgI        BsiEI       BsiWI       BsmBI       BsmFI       
BsmI        BsoBI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrDI       BsrFI       BsrGI       
BsrI        BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstXI       
BstYI       BstZ17I     Bsu36I      CelII       CfoI        
Cfr10I      ClaI        CpoI        DraI        DraIII      
DrdI        EaeI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO65I     
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
FspI        HaeII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HindIII     HpaI        
KasI        KpnI        KspI        MamI        MfeI        
MflI        MluI        MluNI       MroI        MscI        
MslI        MunI        MvnI        NaeI        NarI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflMI       PinAI       PmeI        PshAI       PshBI       
Psp1406I    PvuI        PvuII       RcaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        TaqI        
TthHB8I     Van91I      XbaI        XcmI        XhoI        
XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AccI           26  GTCTAC               GT^[AC][GT]AC
Alw44I        115  GTGCAC               G^TGCAC
ApaLI         115  GTGCAC               G^TGCAC
Asp700I       482  GAAAAGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         115  GTGCAC               G[AT]GC[AT]^C
AspI           75  GACCAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaII          80  GGTCC                G^G[AT]CC
BanII          94  GAGCCC               G[GA]GC[CT]^C
BbrPI         112  CACGTG               CAC^GTG
BfaI           23  CTAG                 C^TAG
BpmI           91  CTGGAG               CTGGAG(16/14)
BsaAI         112  CACGTG               [CT]AC^GT[GA]
BsaI          463  GAGACC               GGTCTC(1/5)
BsiHKAI       115  GTGCAC               G[AT]GC[AT]^C
BspMI         431  ACCTGC               ACCTGC(4/8)
BsrBI         298  GAGCGG               CCGCTC(-3/-3)
BstF5I        137  CATCC                GGATG(2/0)
DpnII         414  GATC                 ^GATC
EarI          440  CTCTTC               CTCTTC(1/4)
EcoT14I        98  CCATGG               C^C[AT][AT]GG
FokI          137  CATCC                GGATG(9/13)
Ksp632I       440  CTCTTC               CTCTTC(1/4)
MaeI           23  CTAG                 C^TAG
MaeII         113  ACGT                 A^CGT
MaeIII        103  GTCAC                ^GT[ACGT]AC
MboI          414  GATC                 ^GATC
MseI          425  TTAA                 T^TAA
MspA1I        310  CAGCGG               C[AC]G^C[GT]G
NcoI           98  CCATGG               C^CATGG
NdeII         414  GATC                 ^GATC
PflFI          75  GACCAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI         112  CACGTG               CAC^GTG
PmlI          112  CACGTG               CAC^GTG
PpuMI          79  AGGTCCT              [GA]G^G[AT]CC[CT]
PstI          433  CTGCAG               CTGCA^G
Sau3AI        414  GATC                 ^GATC
ScaI          260  AGTACT               AGT^ACT
SexAI          76  ACCAGGT              A^CC[AT]GGT
SfaNI         231  GATGC                GCATC(5/9)
SfcI          433  CTGCAG               C^T[GA][CT]AG
StyI           98  CCATGG               C^C[AT][AT]GG
TfiI           55  GATTC                G^A[AT]TC
Tru9I         425  TTAA                 T^TAA
Tsp45I        103  GTCAC                ^GT[GC]AC
Tth111I        75  GACCAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmnI          482  GAAAAGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

BcnI          129  CCCGG                CC^[GC]GG
              284  CCCGG               
BmyI           94  GAGCCC               G[AGT]GC[ACT]^C
              115  GTGCAC              
BseRI         151  GAGGAG               GAGGAG(10/8)
              364  GAGGAG              
BsiYI         278  CCTTCACCCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              284  CCCGGAAAAGG         
BslI          278  CCTTCACCCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              284  CCCGGAAAAGG         
BsmAI         463  GAGAC                GTCTC(1/5)
              438  GTCTC               
Bsp1286I       94  GAGCCC               G[AGT]GC[ACT]^C
              115  GTGCAC              
DraII          79  AGGTCCT              [GA]G^G[ACGT]CC[CT]
              125  AGGCCCC             
DsaI           98  CCATGG               C^C[GA][CT]GG
              160  CCACGG              
EcoO109I       79  AGGTCCT              [GA]G^G[ACGT]CC[CT]
              125  AGGCCCC             
HaeIII        126  GGCC                 GG^CC
              272  GGCC                
HapII         130  CCGG                 C^CGG
              285  CCGG                
HpaII         130  CCGG                 C^CGG
              285  CCGG                
HphI          315  GGTGA                GGTGA(8/7)
              281  TCACC               
MboII          47  TCTTC                GAAGA(8/7)
              441  TCTTC               
MspI          130  CCGG                 C^CGG
              285  CCGG                
NciI          129  CCCGG                CC^[GC]GG
              284  CCCGG               
NlaIV          93  GGAGCC               GG[ACGT]^[ACGT]CC
              126  GGCCCC              
PleI          181  GACTC                GAGTC(4/5)
              383  GACTC               
TspRI          71  CACTG                CA[GC]TG[ACGT][ACGT]^
              204  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   24 enzymes) #
#########################################

AciI        AfaI        AluI        BbvI        BsaJI       
BssKI       BstNI       Cac8I       Cfr13I      DdeI        
EcoRII      FauI        Fnu4HI      HinfI       ItaI        
MnlI        MvaI        MwoI        NlaIII      RsaI        
Sau96I      ScrFI       TseI        Tsp509I     
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