Restriction map for
pF1KB9580
(Circular) (Six-base) MAPSORT of: pF1KB9580.seq Check: 6479 from: 1 to: 3656
pF1KB9580 3656 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 135 694 135
Size: 559 3097
Acc65I G'GTAC_C
Cuts at: 672 672
Size: 3656
AclI AA'CG_TT
Cuts at: 3496 3496
Size: 3656
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1481 1913 2660 2928 1481
Size: 432 747 268 2209
Fragments arranged by size:
2209 747 432 268
AflIII A'CryG_T
Cuts at: 1008 2112 3089 1008
Size: 1104 977 1575
Fragments arranged by size:
1575 1104 977
AgeI A'CCGG_T
Cuts at: 2044 2044
Size: 3656
AlwNI CAG_nnn'CTG
Cuts at: 961 2528 961
Size: 1567 2089
ApaLI G'TGCA_C
Cuts at: 223 2426 223
Size: 2203 1453
ApoI r'AATT_y
Cuts at: 660 3009 3265 660
Size: 2349 256 1051
Fragments arranged by size:
2349 1051 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3656
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3656
AvaI C'yCGr_G
Cuts at: 676 676
Size: 3656
BamHI G'GATC_C
Cuts at: 681 681
Size: 3656
BanI G'GyrC_C
Cuts at: 672 1086 1334 1369 672
Size: 414 248 35 2959
Fragments arranged by size:
2959 414 248 35
BanII G_rGCy'C
Cuts at: 206 670 1700 206
Size: 464 1030 2162
Fragments arranged by size:
2162 1030 464
BbeI G_GCGC'C
Cuts at: 1090 1338 1090
Size: 248 3408
BciVI GTATCCnnnnn_n'
Cuts at: 1554 2315 3656 1554
Size: 761 1341 1554
Fragments arranged by size:
1554 1341 761
BclI T'GATC_A
Cuts at: 1176 1176
Size: 3656
BglII A'GATC_T
Cuts at: 1171 3208 1171
Size: 2037 1619
BlpI GC'TnA_GC
Cuts at: 768 768
Size: 3656
Bme1580I G_kGCm'C
Cuts at: 227 1281 1374 2430 227
Size: 1054 93 1056 1453
Fragments arranged by size:
1453 1056 1054 93
BmrI ACTGGGnnnn_n'
Cuts at: 868 949 1284 3428 868
Size: 81 335 2144 1096
Fragments arranged by size:
2144 1096 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2023 2023
Size: 3656
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 220 220
Size: 3656
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 832 1711 2203 2501 832
Size: 879 492 298 1987
Fragments arranged by size:
1987 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 565 3360 565
Size: 2795 861
BsaAI yAC'GTr
Cuts at: 222 1009 222
Size: 787 2869
BsaBI GATnn'nnATC
Cuts at: 6 1170 3207 6
Size: 1164 2037 455
Fragments arranged by size:
2037 1164 455
BsaHI Gr'CG_yC
Cuts at: 1087 1335 2037 3533 1087
Size: 248 702 1496 1210
Fragments arranged by size:
1496 1210 702 248
BsaWI w'CCGG_w
Cuts at: 845 1366 2044 2318 2465 2876 845
Size: 521 678 274 147 411 1625
Fragments arranged by size:
1625 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 274 487 274
Size: 213 3443
BseYI C'CCAG_C
Cuts at: 283 449 1081 1839 2416 2912 283
Size: 166 632 758 577 496 1027
Fragments arranged by size:
1027 758 632 577 496 166
BsiEI CG_ry'CG
Cuts at: 104 1198 1244 2452 104
Size: 1094 46 1208 1308
Fragments arranged by size:
1308 1208 1094 46
BsiHKAI G_wGCw'C
Cuts at: 227 670 1448 1638 2430 227
Size: 443 778 190 792 1453
Fragments arranged by size:
1453 792 778 443 190
Bsp1286I G_dGCh'C
Cuts at: 206 227 670 1281 1374 1448 1638 1700 2430
Size: 21 443 611 93 74 190 62 730
Cuts at: 2430 206
Size: 1432
Fragments arranged by size:
1432 730 611 443 190 93 74 62
21
BspEI T'CCGG_A
Cuts at: 2876 2876
Size: 3656
BspHI T'CATG_A
Cuts at: 2832 2832
Size: 3656
BspMI ACCTGCnnnn'nnnn_
Cuts at: 548 706 920 1222 1603 548
Size: 158 214 302 381 2601
Fragments arranged by size:
2601 381 302 214 158
BsrBI CCG'CTC
Cuts at: 50 408 1948 2002 3486 3651 50
Size: 358 1540 54 1484 165 55
Fragments arranged by size:
1540 1484 358 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1568 1568
Size: 3656
BsrFI r'CCGG_y
Cuts at: 1654 1835 2044 1654
Size: 181 209 3266
Fragments arranged by size:
3266 209 181
BssSI C'ACGA_G
Cuts at: 1927 2285 1927
Size: 358 3298
BstAPI GCAn_nnn'nTGC
Cuts at: 1033 1033
Size: 3656
BstBI TT'CG_AA
Cuts at: 2017 2017
Size: 3656
BstYI r'GATC_y
Cuts at: 1 681 1148 1171 1506 1752 2764 2850 2862
Size: 680 467 23 335 246 1012 86 12
Cuts at: 2862 3208 1
Size: 346 449
Fragments arranged by size:
1012 680 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 206 268 206
Size: 62 3594
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1606 1787 1950 1606
Size: 181 163 3312
Fragments arranged by size:
3312 181 163
BtsI GCAGTG_nn'
Cuts at: 899 899
Size: 3656
DraI TTT'AAA
Cuts at: 655 655
Size: 3656
DrdI GACnn_nn'nnGTC
Cuts at: 1362 2220 1362
Size: 858 2798
EaeI y'GGCC_r
Cuts at: 1241 1415 1806 1833 3178 1241
Size: 174 391 27 1345 1719
Fragments arranged by size:
1719 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1241 1241
Size: 3656
EarI CTCTTCn'nnn_
Cuts at: 554 1679 1889 554
Size: 1125 210 2321
Fragments arranged by size:
2321 1125 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 445 1864 2174 2320 3466 445
Size: 1419 310 146 1146 635
Fragments arranged by size:
1419 1146 635 310 146
EcoICRI GAG'CTC
Cuts at: 668 668
Size: 3656
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 220 1481 1913 2660 2928 220
Size: 1261 432 747 268 948
Fragments arranged by size:
1261 948 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 188 234 795 188
Size: 46 561 3049
Fragments arranged by size:
3049 561 46
EcoRI G'AATT_C
Cuts at: 660 660
Size: 3656
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1675 1675
Size: 3656
FspI TGC'GCA
Cuts at: 1437 1437
Size: 3656
HaeII r_GCGC'y
Cuts at: 1090 1338 2360 3339 1090
Size: 248 1022 979 1407
Fragments arranged by size:
1407 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 330 362 506 538 1498 1528 1530 1560 330
Size: 32 144 32 960 30 2 30 2426
Fragments arranged by size:
2426 960 144 32 32 30 30 2
HincII GTy'rAC
Cuts at: 695 695
Size: 3656
KasI G'GCGC_C
Cuts at: 1086 1334 1086
Size: 248 3408
KpnI G_GTAC'C
Cuts at: 676 676
Size: 3656
MluI A'CGCG_T
Cuts at: 3089 3089
Size: 3656
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2327 2511 2327
Size: 184 3472
MscI TGG'CCA
Cuts at: 1417 1417
Size: 3656
MslI CAynn'nnrTG
Cuts at: 1645 1772 1645
Size: 127 3529
MspA1I CmG'CkG
Cuts at: 420 767 1081 1441 2454 2699 420
Size: 347 314 360 1013 245 1377
Fragments arranged by size:
1377 1013 360 347 314 245
NaeI GCC'GGC
Cuts at: 1837 1837
Size: 3656
NarI GG'CG_CC
Cuts at: 1087 1335 1087
Size: 248 3408
NcoI C'CATG_G
Cuts at: 206 206
Size: 3656
NgoMIV G'CCGG_C
Cuts at: 1835 1835
Size: 3656
NspI r_CATG'y
Cuts at: 709 1209 2116 709
Size: 500 907 2249
Fragments arranged by size:
2249 907 500
PciI A'CATG_T
Cuts at: 2112 2112
Size: 3656
PmeI GTTT'AAAC
Cuts at: 655 655
Size: 3656
PmlI CAC'GTG
Cuts at: 222 222
Size: 3656
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2795 2827 2795
Size: 32 3624
PpuMI rG'GwC_Cy
Cuts at: 188 188
Size: 3656
PstI C_TGCA'G
Cuts at: 545 703 1388 545
Size: 158 685 2813
Fragments arranged by size:
2813 685 158
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3656
PvuII CAG'CTG
Cuts at: 1081 1441 1081
Size: 360 3296
RsrII CG'GwC_CG
Cuts at: 1851 1851
Size: 3656
SacI G_AGCT'C
Cuts at: 670 670
Size: 3656
SalI G'TCGA_C
Cuts at: 693 693
Size: 3656
SapI GCTCTTCn'nnn_
Cuts at: 1679 1889 1679
Size: 210 3446
SbfI CC_TGCA'GG
Cuts at: 703 703
Size: 3656
ScaI AGT'ACT
Cuts at: 370 3173 370
Size: 2803 853
SexAI A'CCwGG_T
Cuts at: 184 184
Size: 3656
SfcI C'TryA_G
Cuts at: 33 541 699 1384 2377 2568 33
Size: 508 158 685 993 191 1121
Fragments arranged by size:
1121 993 685 508 191 158
SfoI GGC'GCC
Cuts at: 1088 1336 1088
Size: 248 3408
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3656
SmaI CCC'GGG
Cuts at: 678 678
Size: 3656
SmlI C'TyrA_G
Cuts at: 811 1726 2218 2480 811
Size: 915 492 262 1987
Fragments arranged by size:
1987 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3656
SphI G_CATG'C
Cuts at: 709 1209 709
Size: 500 3156
StyI C'CwwG_G
Cuts at: 206 790 1144 206
Size: 584 354 2718
Fragments arranged by size:
2718 584 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 242 1239 1372 1831 2040 242
Size: 997 133 459 209 1858
Fragments arranged by size:
1858 997 459 209 133
TatI w'GTAC_w
Cuts at: 368 527 3171 368
Size: 159 2644 853
Fragments arranged by size:
2644 853 159
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3173 3205 3173
Size: 32 3624
Tth111I GACn'n_nGTC
Cuts at: 186 1453 186
Size: 1267 2389
XbaI T'CTAG_A
Cuts at: 687 687
Size: 3656
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1151 1151
Size: 3656
XmaI C'CCGG_G
Cuts at: 676 676
Size: 3656
XmnI GAAnn'nnTTC
Cuts at: 594 594
Size: 3656
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PmeI PmlI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmgBI BmtI Bpu10I BsaXI BsgI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI ZraI