Restriction map for pF1KB9542
##################################
# NO CUT ENZYMES (  144 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AflII       AhdI        
Alw44I      AlwI        AocI        ApaI        ApaLI       
ApoI        AscI        Asp700I     Asp718I     AspEI       
AspI        AvaI        AviII       AvrII       BalI        
BamHI       BanII       BbeI        BbrPI       BbsI        
BbvCI       BcgI        BciVI       BclI        BcnI        
BfrI        BlnI        BlpI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaHI       BseAI       BsgI        
BsiEI       BsiWI       BsmI        BsoBI       Bsp1407I    
BspDI       BspEI       BspHI       BspMI       BsrBI       
BsrDI       BsrGI       BssHII      Bst1107I    BstAPI      
BstBI       BstUI       BstZ17I     Bsu36I      CelII       
ClaI        CpoI        DraI        DrdI        EaeI        
EagI        Eam1105I    EclXI       Eco52I      Eco81I      
EcoRI       EcoRV       EcoT22I     FauI        FbaI        
FseI        FspI        HgaI        Hin1I       HincII      
HindII      HpaI        KasI        KpnI        KspI        
MaeII       MamI        MfeI        MluI        MluNI       
MroI        MscI        MspA1I      MunI        MvnI        
NarI        NciI        NdeI        NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PmaCI       PmeI        PmlI        PshAI       
Psp1406I    PvuI        PvuII       RcaI        RsrII       
SacI        SacII       SalI        SapI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        SspBI       
SspI        SwaI        Tsp45I      Tth111I     XbaI        
XhoI        XmaCI       XmaI        XmnI        


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# ONE CUT ENZYMES (   59 enzymes) #
###################################

AgeI          791  ACCGGT               A^CCGGT
AlwNI         640  CAGGCTCTG            CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI       516  AGCGCT               AGC^GCT
AspHI         779  GTGCTC               G[AT]GC[AT]^C
BanI          763  GGTGCC               G^G[CT][GA]CC
BfaI          128  CTAG                 C^TAG
BglI          393  GCCAGCAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         474  AGATCT               A^GATCT
BmyI          779  GTGCTC               G[AGT]GC[ACT]^C
BpmI          709  CTGGAG               CTGGAG(16/14)
BsaWI         791  ACCGGT               [AT]^CCGG[AT]
BseRI         826  GAGGAG               GAGGAG(10/8)
BsiHKAI       779  GTGCTC               G[AT]GC[AT]^C
BsmBI          18  GAGACG               CGTCTC(1/5)
Bsp1286I      779  GTGCTC               G[AGT]GC[ACT]^C
BsrI          212  CCAGT                ACTGG(1/-1)
BssSI         823  CACGAG               CACGAG(-5/-1)
BstEII        634  GGTTACC              G^GT[ACGT]ACC
BstPI         634  GGTTACC              G^GT[ACGT]ACC
BstYI         474  AGATCT               [GA]^GATC[CT]
CfoI          517  GCGC                 GCG^C
DpnII         475  GATC                 ^GATC
DraII         688  GGGACCC              [GA]G^G[ACGT]CC[CT]
DraIII        652  CACGCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI          615  CCATGG               C^C[GA][CT]GG
EarI          306  GAAGAG               CTCTTC(1/4)
Eco47III      516  AGCGCT               AGC^GCT
EcoNI         412  CCTGCAGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      688  GGGACCC              [GA]G^G[ACGT]CC[CT]
EcoO65I       634  GGTTACC              G^GT[ACGT]ACC
EcoT14I       615  CCATGG               C^C[AT][AT]GG
HaeII         516  AGCGCT               [GA]GCGC^[CT]
HhaI          517  GCGC                 GCG^C
HinP1I        517  GCGC                 G^CGC
HindIII       232  AAGCTT               A^AGCTT
HphI          110  TCACC                GGTGA(8/7)
Ksp632I       306  GAAGAG               CTCTTC(1/4)
MaeI          128  CTAG                 C^TAG
MaeIII        635  GTTAC                ^GT[ACGT]AC
MboI          475  GATC                 ^GATC
MflI          474  AGATCT               [GA]^GATC[CT]
NaeI          102  GCCGGC               GCC^GGC
NcoI          615  CCATGG               C^CATGG
NdeII         475  GATC                 ^GATC
NgoMIV        102  GCCGGC               G^CCGGC
PinAI         791  ACCGGT               A^CCGGT
PpuMI         688  GGGACCC              [GA]G^G[AT]CC[CT]
PstI          413  CTGCAG               CTGCA^G
Sau3AI        475  GATC                 ^GATC
SbfI          412  CCTGCAGG             CCTGCA^GG
SfcI          413  CTGCAG               C^T[GA][CT]AG
Sse8387I      412  CCTGCAGG             CCTGCA^GG
StuI          409  AGGCCT               AGG^CCT
StyI          615  CCATGG               C^C[AT][AT]GG
TaqI          304  TCGA                 T^CGA
Tsp509I       207  AATT                 ^AATT
TthHB8I       304  TCGA                 T^CGA
XcmI          113  CCAACATCTGGATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         474  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AciI          468  CCGC                 CCGC(-3/-1)
              246  GCGG                
AflIII        133  ACATGT               A^C[GA][CT]GT
              498  ACATGT              
AseI          151  ATTAAT               AT^TAAT
              204  ATTAAT              
AsnI          151  ATTAAT               AT^TAAT
              204  ATTAAT              
AvaII         689  GGACC                G^G[AT]CC
              789  GGACC               
BsaI           23  GAGACC               GGTCTC(1/5)
              619  GGTCTC              
BspLU11I      133  ACATGT               A^CATGT
              498  ACATGT              
BsrFI         102  GCCGGC               [GA]^CCGG[CT]
              791  ACCGGT              
BstXI         525  CCAGGACAGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              667  CCAATGCTGTGG        
Cfr10I        102  GCCGGC               [GA]^CCGG[CT]
              791  ACCGGT              
DdeI          241  CTCAG                C^T[ACGT]AG
              782  CTCAG               
HapII         103  CCGG                 C^CGG
              792  CCGG                
HpaII         103  CCGG                 C^CGG
              792  CCGG                
MboII         255  GAAGA                GAAGA(8/7)
              306  GAAGA               
MslI          117  CATCTGGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              272  CACCACCATG          
MspI          103  CCGG                 C^CGG
              792  CCGG                
NspI          133  ACATGT               [GA]CATG^[CT]
              498  ACATGT              
PflMI         113  CCAACATCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              651  CCACGCTGTGG         
PleI           47  GACTC                GAGTC(4/5)
               97  GACTC               
PshBI         151  ATTAAT               AT^TAAT
              204  ATTAAT              
SfaNI          32  GCATC                GCATC(5/9)
              422  GCATC               
SmlI          478  CTTGAG               C^T[CT][GA]AG
              838  CTCAAG              
TfiI          328  GATTC                G^A[AT]TC
              678  GAATC               
TspRI         332  CAGTG                CA[GC]TG[ACGT][ACGT]^
              531  CAGTG               
Van91I        113  CCAACATCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              651  CCACGCTGTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   30 enzymes) #
#########################################

AfaI        AluI        BbvI        BsaJI       BsiYI       
BslI        BsmAI       BsmFI       BssKI       BstF5I      
BstNI       Cac8I       Cfr13I      EcoRII      Fnu4HI      
FokI        HaeIII      HinfI       ItaI        MnlI        
MseI        MvaI        MwoI        NlaIII      NlaIV       
RsaI        Sau96I      ScrFI       Tru9I       TseI        
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