Restriction map for pF1KB9542
 (Linear) (Six-base) MAPSORT of: KIBB9542.seq  Check: 8857  from: 1  to: 870



KIBB9542.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:10  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      64     274     668     794     870 
   Size:         64     210     394     126      76

  Fragments arranged by size: 

                 394     210     126      76      64
 
AfeI AGC'GCT

Cuts at:      0     518     870 
   Size:        518     352
 
AflIII A'CryG_T

Cuts at:      0     133     498     870 
   Size:        133     365     372

  Fragments arranged by size: 

                 372     365     133
 
AgeI A'CCGG_T

Cuts at:      0     791     870 
   Size:        791      79
 
AlwNI CAG_nnn'CTG

Cuts at:      0     645     870 
   Size:        645     225
 
AseI AT'TA_AT

Cuts at:      0     152     205     870 
   Size:        152      53     665

  Fragments arranged by size: 

                 665     152      53
 
BanI G'GyrC_C

Cuts at:      0     763     870 
   Size:        763     107
 
BglI GCCn_nnn'nGGC

Cuts at:      0     399     870 
   Size:        399     471
 
BglII A'GATC_T

Cuts at:      0     474     870 
   Size:        474     396
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     730     870 
   Size:        730     140
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     499     823     870 
   Size:        499     324      47

  Fragments arranged by size: 

                 499     324      47
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      17     625     870 
   Size:         17     608     245

  Fragments arranged by size: 

                 608     245      17
 
BsaWI w'CCGG_w

Cuts at:      0     791     870 
   Size:        791      79
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     841     870 
   Size:        841      29
 
BseYI C'CCAG_C

Cuts at:      0      28     159     347     714     870 
   Size:         28     131     188     367     156

  Fragments arranged by size: 

                 367     188     156     131      28
 
BsiHKAI G_wGCw'C

Cuts at:      0     783     870 
   Size:        783      87
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      12     870 
   Size:         12     858
 
Bsp1286I G_dGCh'C

Cuts at:      0     783     870 
   Size:        783      87
 
BsrFI r'CCGG_y

Cuts at:      0     102     791     870 
   Size:        102     689      79

  Fragments arranged by size: 

                 689     102      79
 
BssSI C'ACGA_G

Cuts at:      0     823     870 
   Size:        823      47
 
BstEII G'GTnAC_C

Cuts at:      0     634     870 
   Size:        634     236
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     532     674     870 
   Size:        532     142     196

  Fragments arranged by size: 

                 532     196     142
 
BstYI r'GATC_y

Cuts at:      0     474     870 
   Size:        474     396
 
BtgI C'CryG_G

Cuts at:      0     615     870 
   Size:        615     255
 
DraIII CAC_nnn'GTG

Cuts at:      0     657     870 
   Size:        657     213
 
EarI CTCTTCn'nnn_

Cuts at:      0     301     870 
   Size:        301     569
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     457     870 
   Size:        457     413
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      64     274     668     730     794     870 
   Size:         64     210     394      62      64      76

  Fragments arranged by size: 

                 394     210      76      64      64      62
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     416     870 
   Size:        416     454
 
EcoO109I rG'GnC_Cy

Cuts at:      0     689     870 
   Size:        689     181
 
HaeII r_GCGC'y

Cuts at:      0     520     870 
   Size:        520     350
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      44      76     870 
   Size:         44      32     794

  Fragments arranged by size: 

                 794      44      32
 
HindIII A'AGCT_T

Cuts at:      0     232     870 
   Size:        232     638
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      75     575     870 
   Size:         75     500     295

  Fragments arranged by size: 

                 500     295      75
 
MslI CAynn'nnrTG

Cuts at:      0     121     276     870 
   Size:        121     155     594

  Fragments arranged by size: 

                 594     155     121
 
NaeI GCC'GGC

Cuts at:      0     104     870 
   Size:        104     766
 
NcoI C'CATG_G

Cuts at:      0     615     870 
   Size:        615     255
 
NgoMIV G'CCGG_C

Cuts at:      0     102     870 
   Size:        102     768
 
NspI r_CATG'y

Cuts at:      0     137     502     870 
   Size:        137     365     368

  Fragments arranged by size: 

                 368     365     137
 
PciI A'CATG_T

Cuts at:      0     133     498     870 
   Size:        133     365     372

  Fragments arranged by size: 

                 372     365     133
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     119     657     870 
   Size:        119     538     213

  Fragments arranged by size: 

                 538     213     119
 
PpuMI rG'GwC_Cy

Cuts at:      0     689     870 
   Size:        689     181
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     426     458     870 
   Size:        426      32     412

  Fragments arranged by size: 

                 426     412      32
 
PstI C_TGCA'G

Cuts at:      0     417     870 
   Size:        417     453
 
SanDI GG'GwC_CC

Cuts at:      0     689     870 
   Size:        689     181
 
SbfI CC_TGCA'GG

Cuts at:      0     417     870 
   Size:        417     453
 
SfcI C'TryA_G

Cuts at:      0     413     870 
   Size:        413     457
 
SmlI C'TyrA_G

Cuts at:      0     478     838     870 
   Size:        478     360      32

  Fragments arranged by size: 

                 478     360      32
 
StuI AGG'CCT

Cuts at:      0     411     870 
   Size:        411     459
 
StyI C'CwwG_G

Cuts at:      0     615     870 
   Size:        615     255
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      20     361     526     870 
   Size:         20     341     165     344

  Fragments arranged by size: 

                 344     341     165      20
 
TatI w'GTAC_w

Cuts at:      0     237     834     870 
   Size:        237     597      36

  Fragments arranged by size: 

                 597     237      36
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     120     870 
   Size:        120     750
 

 Enzymes that do cut:

     AcuI     AfeI   AflIII     AgeI    AlwNI     AseI     BanI     BglI
    BglII     BpmI    BpuEI     BsaI    BsaWI    BseRI    BseYI  BsiHKAI
    BsmBI Bsp1286I    BsrFI    BssSI   BstEII    BstXI    BstYI     BtgI
   DraIII     EarI     EciI  Eco57MI    EcoNI EcoO109I    HaeII    Hin4I
  HindIII     MmeI     MslI     NaeI     NcoI   NgoMIV     NspI     PciI
    PflMI    PpuMI     PsrI     PstI    SanDI     SbfI     SfcI     SmlI
     StuI     StyI    TaqII     TatI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI    AflII     AhdI     AleI
     AlfI     AloI     ApaI    ApaLI     ApoI     AscI    AsiSI     AvaI
    AvrII     BaeI    BamHI    BanII     BbeI     BbsI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BplI   Bpu10I    BsaAI    BsaBI    BsaHI    BsaXI     BsgI    BsiEI
    BsiWI     BsmI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrGI
   BssHII   BstAPI    BstBI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI
    CspCI     DraI     DrdI     EaeI     EagI  EcoICRI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI   HincII     HpaI     KasI     KpnI
     MfeI     MluI     MscI   MspA1I     NarI     NdeI     NheI     NotI
     NruI     NsiI     PacI     PasI     PfoI     PmeI     PmlI     PpiI
    PshAI     PsiI   PspOMI    PspXI     PvuI    PvuII    RsrII     SacI
    SacII     SalI     SapI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI
     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI
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