################################## # NO CUT ENZYMES ( 152 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AvaI AviII BalI BamHI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BmyI BpuAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstF5I BstPI BstUI BstZ17I Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT22I FbaI FokI FseI FspI HaeII HgaI Hin1I HpaI KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflMI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Van91I XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AflII 805 CTTAAG C^TTAAG AocI 451 CCTAAGG CC^T[ACGT]AGG AspI 931 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 133 CCTAGG C^CTAGG BanI 1039 GGTGCC G^G[CT][GA]CC BbrPI 108 CACGTG CAC^GTG BcnI 627 CCGGG CC^[GC]GG BfrI 805 CTTAAG C^TTAAG BlnI 133 CCTAGG C^CTAGG BspMI 393 ACCTGC ACCTGC(4/8) BsrDI 775 GCAATG GCAATG(2/0) BstAPI 964 GCAGCCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 172 CCAGCTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 194 GGATCT [GA]^GATC[CT] Bsu36I 451 CCTAAGG CC^T[ACGT]AGG CfoI 290 GCGC GCG^C DraII 356 AGGTCCT [GA]G^G[ACGT]CC[CT] EarI 545 CTCTTC CTCTTC(1/4) Eco81I 451 CCTAAGG CC^T[ACGT]AGG EcoNI 78 CCTGCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 356 AGGTCCT [GA]G^G[ACGT]CC[CT] HaeIII 1113 GGCC GG^CC HapII 627 CCGG C^CGG HhaI 290 GCGC GCG^C HinP1I 290 GCGC G^CGC HindIII 1089 AAGCTT A^AGCTT HpaII 627 CCGG C^CGG Ksp632I 545 CTCTTC CTCTTC(1/4) MflI 194 GGATCT [GA]^GATC[CT] MspI 627 CCGG C^CGG NciI 627 CCGGG CC^[GC]GG PflFI 931 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 108 CACGTG CAC^GTG PmlI 108 CACGTG CAC^GTG PpuMI 356 AGGTCCT [GA]G^G[AT]CC[CT] ScaI 1015 AGTACT AGT^ACT SexAI 44 ACCAGGT A^CC[AT]GGT SfaNI 538 GATGC GCATC(5/9) SmlI 805 CTTAAG C^T[CT][GA]AG TaqI 514 TCGA T^CGA Tth111I 931 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 514 TCGA T^CGA XbaI 492 TCTAGA T^CTAGA XcmI 263 CCAAAATCAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 194 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AlwNI 398 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG 827 CAGAACCTG BfaI 134 CTAG C^TAG 493 CTAG BlpI 66 GCTAAGC GC^T[ACGT]AGC 340 GCTCAGC BpmI 680 CTCCAG CTGGAG(16/14) 1098 CTCCAG Bpu1102I 66 GCTAAGC GC^T[ACGT]AGC 340 GCTCAGC BsaAI 108 CACGTG [CT]AC^GT[GA] 757 CACGTA BsiYI 78 CCTGCTGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 611 CCAGTGTTGGG BslI 78 CCTGCTGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 611 CCAGTGTTGGG CelII 66 GCTAAGC GC^T[ACGT]AGC 340 GCTCAGC EcoT14I 133 CCTAGG C^C[AT][AT]GG 482 CCTTGG HincII 3 GTCAAC GT[CT]^[GA]AC 477 GTTGAC HindII 3 GTCAAC GT[CT]^[GA]AC 477 GTTGAC HinfI 168 GAGTC G^A[ACGT]TC 516 GAGTC MaeI 134 CTAG C^TAG 493 CTAG MaeII 109 ACGT A^CGT 758 ACGT NlaIV 41 GGAACC GG[ACGT]^[ACGT]CC 1039 GGTGCC PleI 168 GAGTC GAGTC(4/5) 516 GAGTC StyI 133 CCTAGG C^C[AT][AT]GG 482 CCTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 43 enzymes) # ######################################### AciI AfaI AluI AlwI AvaII BbvI BsaJI BsmFI BsrI BssKI BstNI Cac8I Cfr13I DdeI DpnII EcoRII FauI Fnu4HI HphI ItaI MaeIII MboI MboII MnlI MseI MslI MspA1I MvaI MwoI NdeII NlaIII PstI PvuII RsaI Sau3AI Sau96I ScrFI SfcI Tru9I TseI Tsp45I Tsp509I TspRI