(Circular) (Six-base) MAPSORT of: pF1KB9510.seq Check: 4525 from: 1 to: 4229 pF1KB9510 4229 bp With 182 enzymes: SgfI * June 20, 2008 10:31 .. AccI GT'mk_AC Cuts at: 1267 1267 Size: 4229 Acc65I G'GTAC_C Cuts at: 1245 1245 Size: 4229 AclI AA'CG_TT Cuts at: 4069 4069 Size: 4229 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 218 2054 2486 3233 3501 218 Size: 1836 432 747 268 946 Fragments arranged by size: 1836 946 747 432 268 AflII C'TTAA_G Cuts at: 913 913 Size: 4229 AflIII A'CryG_T Cuts at: 1581 2685 3662 1581 Size: 1104 977 2148 Fragments arranged by size: 2148 1104 977 AgeI A'CCGG_T Cuts at: 2617 2617 Size: 4229 AleI CACnn'nnGTG Cuts at: 216 216 Size: 4229 AlwNI CAG_nnn'CTG Cuts at: 511 940 1534 3101 511 Size: 429 594 1567 1639 Fragments arranged by size: 1639 1567 594 429 ApaLI G'TGCA_C Cuts at: 2999 2999 Size: 4229 ApoI r'AATT_y Cuts at: 1233 3582 3838 1233 Size: 2349 256 1624 Fragments arranged by size: 2349 1624 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4229 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4229 AvaI C'yCGr_G Cuts at: 1249 1249 Size: 4229 AvrII C'CTAG_G Cuts at: 241 241 Size: 4229 BamHI G'GATC_C Cuts at: 1254 1254 Size: 4229 BanI G'GyrC_C Cuts at: 1147 1245 1659 1907 1942 1147 Size: 98 414 248 35 3434 Fragments arranged by size: 3434 414 248 98 35 BanII G_rGCy'C Cuts at: 1243 2273 1243 Size: 1030 3199 BbeI G_GCGC'C Cuts at: 1663 1911 1663 Size: 248 3981 BciVI GTATCCnnnnn_n' Cuts at: 2127 2888 4229 2127 Size: 761 1341 2127 Fragments arranged by size: 2127 1341 761 BclI T'GATC_A Cuts at: 1749 1749 Size: 4229 BglII A'GATC_T Cuts at: 1744 3781 1744 Size: 2037 2192 BlpI GC'TnA_GC Cuts at: 175 449 1341 175 Size: 274 892 3063 Fragments arranged by size: 3063 892 274 Bme1580I G_kGCm'C Cuts at: 1854 1947 3003 1854 Size: 93 1056 3080 Fragments arranged by size: 3080 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 1441 1522 1857 4001 1441 Size: 81 335 2144 1669 Fragments arranged by size: 2144 1669 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2596 2596 Size: 4229 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 773 1191 773 Size: 418 3811 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1405 2284 2776 3074 1405 Size: 879 492 298 2560 Fragments arranged by size: 2560 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 3933 3933 Size: 4229 BsaAI yAC'GTr Cuts at: 218 867 1582 218 Size: 649 715 2865 Fragments arranged by size: 2865 715 649 BsaBI GATnn'nnATC Cuts at: 6 1743 3780 6 Size: 1737 2037 455 Fragments arranged by size: 2037 1737 455 BsaHI Gr'CG_yC Cuts at: 1660 1908 2610 4106 1660 Size: 248 702 1496 1783 Fragments arranged by size: 1783 1496 702 248 BsaWI w'CCGG_w Cuts at: 1418 1939 2617 2891 3038 3449 1418 Size: 521 678 274 147 411 2198 Fragments arranged by size: 2198 678 521 411 274 147 BseYI C'CCAG_C Cuts at: 343 1654 2412 2989 3485 343 Size: 1311 758 577 496 1087 Fragments arranged by size: 1311 1087 758 577 496 BsiEI CG_ry'CG Cuts at: 104 1771 1817 3025 104 Size: 1667 46 1208 1308 Fragments arranged by size: 1667 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 1243 2021 2211 3003 1243 Size: 778 190 792 2469 Fragments arranged by size: 2469 792 778 190 Bsp1286I G_dGCh'C Cuts at: 1243 1854 1947 2021 2211 2273 3003 1243 Size: 611 93 74 190 62 730 2469 Fragments arranged by size: 2469 730 611 190 93 74 62 BspEI T'CCGG_A Cuts at: 3449 3449 Size: 4229 BspHI T'CATG_A Cuts at: 3405 3405 Size: 4229 BspMI ACCTGCnnnn'nnnn_ Cuts at: 510 1279 1493 1795 2176 510 Size: 769 214 302 381 2563 Fragments arranged by size: 2563 769 381 302 214 BsrBI CCG'CTC Cuts at: 50 2521 2575 4059 4224 50 Size: 2471 54 1484 165 55 Fragments arranged by size: 2471 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 890 2141 890 Size: 1251 2978 BsrFI r'CCGG_y Cuts at: 2227 2408 2617 2227 Size: 181 209 3839 Fragments arranged by size: 3839 209 181 BssSI C'ACGA_G Cuts at: 2500 2858 2500 Size: 358 3871 BstAPI GCAn_nnn'nTGC Cuts at: 1078 1606 1078 Size: 528 3701 BstBI TT'CG_AA Cuts at: 2590 2590 Size: 4229 BstXI CCAn_nnnn'nTGG Cuts at: 287 287 Size: 4229 BstYI r'GATC_y Cuts at: 1 302 1254 1721 1744 2079 2325 3337 3423 Size: 301 952 467 23 335 246 1012 86 Cuts at: 3423 3435 3781 1 Size: 12 346 449 Fragments arranged by size: 1012 952 467 449 346 335 301 246 86 23 12 Bsu36I CC'TnA_GG Cuts at: 560 560 Size: 4229 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2179 2360 2523 2179 Size: 181 163 3885 Fragments arranged by size: 3885 181 163 BtsI GCAGTG_nn' Cuts at: 485 1472 485 Size: 987 3242 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 807 842 807 Size: 35 4194 DraI TTT'AAA Cuts at: 1228 1228 Size: 4229 DrdI GACnn_nn'nnGTC Cuts at: 1935 2793 1935 Size: 858 3371 EaeI y'GGCC_r Cuts at: 1814 1988 2379 2406 3751 1814 Size: 174 391 27 1345 2292 Fragments arranged by size: 2292 1345 391 174 27 EagI C'GGCC_G Cuts at: 1814 1814 Size: 4229 EarI CTCTTCn'nnn_ Cuts at: 659 2252 2462 659 Size: 1593 210 2426 Fragments arranged by size: 2426 1593 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2437 2747 2893 4039 2437 Size: 310 146 1146 2627 Fragments arranged by size: 2627 1146 310 146 EcoICRI GAG'CTC Cuts at: 1241 1241 Size: 4229 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 218 773 1191 2054 2486 3233 3501 218 Size: 555 418 863 432 747 268 946 Fragments arranged by size: 946 863 747 555 432 418 268 EcoNI CCTnn'n_nnAGG Cuts at: 190 190 Size: 4229 EcoO109I rG'GnC_Cy Cuts at: 465 1368 465 Size: 903 3326 EcoRI G'AATT_C Cuts at: 1233 1233 Size: 4229 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2248 2248 Size: 4229 FspI TGC'GCA Cuts at: 2010 2010 Size: 4229 HaeII r_GCGC'y Cuts at: 1663 1911 2933 3912 1663 Size: 248 1022 979 1980 Fragments arranged by size: 1980 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 2071 2101 2103 2133 2071 Size: 30 2 30 4167 Fragments arranged by size: 4167 30 30 2 HincII GTy'rAC Cuts at: 113 587 1268 113 Size: 474 681 3074 Fragments arranged by size: 3074 681 474 HindIII A'AGCT_T Cuts at: 1197 1197 Size: 4229 KasI G'GCGC_C Cuts at: 1659 1907 1659 Size: 248 3981 KpnI G_GTAC'C Cuts at: 1249 1249 Size: 4229 MluI A'CGCG_T Cuts at: 3662 3662 Size: 4229 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2900 3084 2900 Size: 184 4045 MscI TGG'CCA Cuts at: 1990 1990 Size: 4229 MslI CAynn'nnrTG Cuts at: 216 869 971 2218 2345 216 Size: 653 102 1247 127 2100 Fragments arranged by size: 2100 1247 653 127 102 MspA1I CmG'CkG Cuts at: 118 453 511 1340 1654 2014 3027 3272 118 Size: 335 58 829 314 360 1013 245 1075 Fragments arranged by size: 1075 1013 829 360 335 314 245 58 NaeI GCC'GGC Cuts at: 2410 2410 Size: 4229 NarI GG'CG_CC Cuts at: 1660 1908 1660 Size: 248 3981 NgoMIV G'CCGG_C Cuts at: 2408 2408 Size: 4229 NspI r_CATG'y Cuts at: 1282 1782 2689 1282 Size: 500 907 2822 Fragments arranged by size: 2822 907 500 PciI A'CATG_T Cuts at: 2685 2685 Size: 4229 PmeI GTTT'AAAC Cuts at: 1228 1228 Size: 4229 PmlI CAC'GTG Cuts at: 218 218 Size: 4229 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3368 3400 3368 Size: 32 4197 PpuMI rG'GwC_Cy Cuts at: 465 465 Size: 4229 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 1082 1114 1082 Size: 32 4197 PstI C_TGCA'G Cuts at: 194 507 1131 1276 1961 194 Size: 313 624 145 685 2462 Fragments arranged by size: 2462 685 624 313 145 PvuI CG_AT'CG Cuts at: 104 104 Size: 4229 PvuII CAG'CTG Cuts at: 118 453 511 1654 2014 118 Size: 335 58 1143 360 2333 Fragments arranged by size: 2333 1143 360 335 58 RsrII CG'GwC_CG Cuts at: 2424 2424 Size: 4229 SacI G_AGCT'C Cuts at: 1243 1243 Size: 4229 SalI G'TCGA_C Cuts at: 1266 1266 Size: 4229 SapI GCTCTTCn'nnn_ Cuts at: 2252 2462 2252 Size: 210 4019 SbfI CC_TGCA'GG Cuts at: 1276 1276 Size: 4229 ScaI AGT'ACT Cuts at: 1125 3746 1125 Size: 2621 1608 SexAI A'CCwGG_T Cuts at: 152 152 Size: 4229 SfcI C'TryA_G Cuts at: 33 190 503 1127 1272 1957 2950 3141 33 Size: 157 313 624 145 685 993 191 1121 Fragments arranged by size: 1121 993 685 624 313 191 157 145 SfoI GGC'GCC Cuts at: 1661 1909 1661 Size: 248 3981 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4229 SmaI CCC'GGG Cuts at: 1251 1251 Size: 4229 SmlI C'TyrA_G Cuts at: 913 1384 2299 2791 3053 913 Size: 471 915 492 262 2089 Fragments arranged by size: 2089 915 492 471 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4229 SphI G_CATG'C Cuts at: 1282 1782 1282 Size: 500 3729 StyI C'CwwG_G Cuts at: 241 590 1363 1717 241 Size: 349 773 354 2753 Fragments arranged by size: 2753 773 354 349 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 228 357 1812 1945 2404 2613 228 Size: 129 1455 133 459 209 1844 Fragments arranged by size: 1844 1455 459 209 133 129 TatI w'GTAC_w Cuts at: 470 1123 3744 470 Size: 653 2621 955 Fragments arranged by size: 2621 955 653 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3746 3778 3746 Size: 32 4197 Tth111I GACn'n_nGTC Cuts at: 1042 2026 1042 Size: 984 3245 XbaI T'CTAG_A Cuts at: 600 1260 600 Size: 660 3569 XcmI CCAnnnn_n'nnnnTGG Cuts at: 378 1724 378 Size: 1346 2883 XmaI C'CCGG_G Cuts at: 1249 1249 Size: 4229 Enzymes that do cut: AccI Acc65I AclI AcuI AflII AflIII AgeI AleI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI PmeI PmlI PpiI PpuMI PsrI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AhdI AlfI AloI ApaI AscI BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI BmtI Bpu10I BsaXI BseRI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I BtgI ClaI DraIII EcoRV FseI FspAI HpaI MfeI NcoI NdeI NheI NotI NruI NsiI PacI PasI PflMI PfoI PshAI PsiI PspOMI PspXI SacII SanDI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI