################################## # NO CUT ENZYMES ( 78 enzymes) # ################################## AatII Acc65I AclI AflII Alw44I Aor51HI ApaLI AscI Asp718I AviII AvrII BalI BamHI BbvCI BcgI BfrI BlnI BlpI Bpu1102I BsiEI BsiWI BsmBI BsmI BspDI BspLU11I BsrDI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I CelII ClaI CpoI DraI DraIII EaeI EagI EclXI Eco47III Eco52I EcoO65I EcoRV FseI FspI HindIII HpaI KpnI KspI MfeI MluI MluNI MscI MunI NdeI NheI NotI NruI NspV PacI PmeI Psp1406I PvuI RsrII SacI SacII SbfI SexAI SfiI SfuI SgrAI SnaBI SpeI Sse8387I SwaI XbaI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AccII 888 CGCG CG^CG AflIII 1394 ACGTGT A^C[GA][CT]GT AgeI 950 ACCGGT A^CCGGT AhdI 3016 GACCGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 3151 CCTCAGG CC^T[ACGT]AGG AseI 165 ATTAAT AT^TAAT AsnI 165 ATTAAT AT^TAAT Asp700I 1279 GAATATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 3016 GACCGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 2302 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC BclI 296 TGATCA T^GATCA BglII 563 AGATCT A^GATCT BsaBI 792 GATTGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 2345 GAGACC GGTCTC(1/5) BstAPI 1968 GCACCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 888 CGCG CG^CG Bsu36I 3151 CCTCAGG CC^T[ACGT]AGG DrdI 2713 GACCTGGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 3016 GACCGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 3151 CCTCAGG CC^T[ACGT]AGG EcoNI 2869 CCTCCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 2554 GAATTC G^AATTC EcoT22I 1408 ATGCAT ATGCA^T FbaI 296 TGATCA T^GATCA MamI 792 GATTGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MvnI 888 CGCG CG^CG NaeI 974 GCCGGC GCC^GGC NgoMIV 974 GCCGGC G^CCGGC NsiI 1408 ATGCAT ATGCA^T NspI 1410 GCATGC [GA]CATG^[CT] PaeR7I 2096 CTCGAG C^TCGAG PflFI 2302 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 950 ACCGGT A^CCGGT PshAI 408 GACCAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 165 ATTAAT AT^TAAT PvuII 2008 CAGCTG CAG^CTG SalI 3272 GTCGAC G^TCGAC SphI 1410 GCATGC GCATG^C Tth111I 2302 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 2096 CTCGAG C^TCGAG XmnI 1279 GAATATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### AccIII 485 TCCGGA T^CCGGA 1638 TCCGGA ApaI 2403 GGGCCC GGGCC^C 2658 GGGCCC BbeI 652 GGCGCC GGCGC^C 1667 GGCGCC BbrPI 421 CACGTG CAC^GTG 1018 CACGTG BbsI 2589 GAAGAC GAAGAC(2/6) 2542 GTCTTC BciVI 2878 GGATAC GTATCC(6/5) 1531 GTATCC BglI 716 GCCTCTCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1932 GCCTTCTTGGC BpuAI 2589 GAAGAC GAAGAC(2/6) 2542 GTCTTC BsaAI 421 CACGTG [CT]AC^GT[GA] 1018 CACGTG BseAI 485 TCCGGA T^CCGGA 1638 TCCGGA Bsp1407I 284 TGTACA T^GTACA 2963 TGTACA BspEI 485 TCCGGA T^CCGGA 1638 TCCGGA BsrBI 233 GAGCGG CCGCTC(-3/-3) 616 GAGCGG BsrFI 950 ACCGGT [GA]^CCGG[CT] 974 GCCGGC BsrGI 284 TGTACA T^GTACA 2963 TGTACA Cfr10I 950 ACCGGT [GA]^CCGG[CT] 974 GCCGGC DsaI 1121 CCATGG C^C[GA][CT]GG 2972 CCATGG HaeII 652 GGCGCC [GA]GCGC^[CT] 1667 GGCGCC KasI 652 GGCGCC G^GCGCC 1667 GGCGCC MroI 485 TCCGGA T^CCGGA 1638 TCCGGA MspA1I 451 CCGCTG C[AC]G^C[GT]G 2008 CAGCTG NarI 652 GGCGCC GG^CGCC 1667 GGCGCC NcoI 1121 CCATGG C^CATGG 2972 CCATGG PmaCI 421 CACGTG CAC^GTG 1018 CACGTG PmlI 421 CACGTG CAC^GTG 1018 CACGTG PpuMI 2711 GGGACCT [GA]G^G[AT]CC[CT] 3056 AGGTCCC ScaI 1316 AGTACT AGT^ACT 2479 AGTACT SfoI 652 GGCGCC GGC^GCC 1667 GGCGCC SmaI 1052 CCCGGG CCC^GGG 1082 CCCGGG SspBI 284 TGTACA T^GTACA 2963 TGTACA SspI 1179 AATATT AAT^ATT 1823 AATATT StuI 461 AGGCCT AGG^CCT 1040 AGGCCT XmaCI 1052 CCCGGG C^CCGGG 1082 CCCGGG XmaI 1052 CCCGGG C^CCGGG 1082 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 105 enzymes) # ######################################### AccI AciI AcsI AcyI AfaI AluI AlwI AlwNI ApoI AspHI AvaI AvaII BanI BanII BbvI BcnI BfaI BmyI BpmI BsaHI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspHI BspMI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PflMI PleI PstI RcaI RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII