(Circular) (Six-base) MAPSORT of: pF1KB9100.seq Check: 710 from: 1 to: 6437 pF1KB9100 6437 bp With 182 enzymes: SgfI * June 20, 2008 10:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2087 2087 Size: 6437 AccI GT'mk_AC Cuts at: 125 3112 3381 3475 125 Size: 2987 269 94 3087 Fragments arranged by size: 3087 2987 269 94 Acc65I G'GTAC_C Cuts at: 3453 3453 Size: 6437 AclI AA'CG_TT Cuts at: 6277 6277 Size: 6437 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 244 1546 2563 2764 4262 4694 5441 5709 244 Size: 1302 1017 201 1498 432 747 268 972 Fragments arranged by size: 1498 1302 1017 972 747 432 268 201 AflIII A'CryG_T Cuts at: 1502 3789 4893 5870 1502 Size: 2287 1104 977 2069 Fragments arranged by size: 2287 2069 1104 977 AgeI A'CCGG_T Cuts at: 1058 4825 1058 Size: 3767 2670 AhdI GACnn_n'nnGTC Cuts at: 3129 3129 Size: 6437 AleI CACnn'nnGTG Cuts at: 164 164 Size: 6437 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1738 1738 Size: 6437 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 589 621 589 Size: 32 6405 AlwNI CAG_nnn'CTG Cuts at: 469 1548 1898 3402 3742 5309 469 Size: 1079 350 1504 340 1567 1597 Fragments arranged by size: 1597 1567 1504 1079 350 340 ApaI G_GGCC'C Cuts at: 2515 2770 2515 Size: 255 6182 ApaLI G'TGCA_C Cuts at: 5207 5207 Size: 6437 ApoI r'AATT_y Cuts at: 1999 2230 2662 2722 3441 5790 6046 1999 Size: 231 432 60 719 2349 256 2390 Fragments arranged by size: 2390 2349 719 432 256 231 60 AseI AT'TA_AT Cuts at: 20 274 20 Size: 254 6183 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 6437 AvaI C'yCGr_G Cuts at: 828 1160 1190 2149 2204 3457 828 Size: 332 30 959 55 1253 3808 Fragments arranged by size: 3808 1253 959 332 55 30 BamHI G'GATC_C Cuts at: 3462 3462 Size: 6437 BanI G'GyrC_C Cuts at: 760 1775 3264 3453 3867 4115 4150 760 Size: 1015 1489 189 414 248 35 3047 Fragments arranged by size: 3047 1489 1015 414 248 189 35 BanII G_rGCy'C Cuts at: 835 2515 2770 2788 3451 4481 835 Size: 1680 255 18 663 1030 2791 Fragments arranged by size: 2791 1680 1030 663 255 18 BbeI G_GCGC'C Cuts at: 764 1779 3871 4119 764 Size: 1015 2092 248 3082 Fragments arranged by size: 3082 2092 1015 248 BbsI GAAGACnn'nnnn_ Cuts at: 2643 2704 2643 Size: 61 6376 BciVI GTATCCnnnnn_n' Cuts at: 1650 2980 4335 5096 6437 1650 Size: 1330 1355 761 1341 1650 Fragments arranged by size: 1650 1355 1341 1330 761 BclI T'GATC_A Cuts at: 404 3957 404 Size: 3553 2884 BfrBI ATG'CAT Cuts at: 1518 1518 Size: 6437 BglI GCCn_nnn'nGGC Cuts at: 830 2046 830 Size: 1216 5221 BglII A'GATC_T Cuts at: 671 3952 5989 671 Size: 3281 2037 1119 Fragments arranged by size: 3281 2037 1119 BlpI GC'TnA_GC Cuts at: 3549 3549 Size: 6437 Bme1580I G_kGCm'C Cuts at: 2515 2770 4062 4155 5211 2515 Size: 255 1292 93 1056 3741 Fragments arranged by size: 3741 1292 1056 255 93 BmgBI CAC'GTC Cuts at: 1503 1503 Size: 6437 BmrI ACTGGGnnnn_n' Cuts at: 1908 2772 2834 2953 3649 3730 4065 6209 1908 Size: 864 62 119 696 81 335 2144 2136 Fragments arranged by size: 2144 2136 864 696 335 119 81 62 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 780 2476 4804 780 Size: 1696 2328 2413 Fragments arranged by size: 2413 2328 1696 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 480 527 586 591 1537 2353 2793 480 Size: 47 59 5 946 816 440 4124 Fragments arranged by size: 4124 946 816 440 59 47 5 Bpu10I CC'TnA_GC Cuts at: 820 2668 3299 820 Size: 1848 631 3958 Fragments arranged by size: 3958 1848 631 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2818 3145 3613 4492 4984 5282 2818 Size: 327 468 879 492 298 3973 Fragments arranged by size: 3973 879 492 468 327 298 BsaI GGTCTCn'nnnn_ Cuts at: 2447 6141 2447 Size: 3694 2743 BsaAI yAC'GTr Cuts at: 531 1128 3790 531 Size: 597 2662 3178 Fragments arranged by size: 3178 2662 597 BsaBI GATnn'nnATC Cuts at: 6 904 3951 5988 6 Size: 898 3047 2037 455 Fragments arranged by size: 3047 2037 898 455 BsaHI Gr'CG_yC Cuts at: 761 1262 1776 2069 3139 3868 4116 4818 6314 Size: 501 514 293 1070 729 248 702 1496 Cuts at: 6314 761 Size: 884 Fragments arranged by size: 1496 1070 884 729 702 514 501 293 248 BsaWI w'CCGG_w Cuts at: 593 1058 1746 3626 4147 4825 5099 5246 5657 Size: 465 688 1880 521 678 274 147 411 Cuts at: 5657 593 Size: 1373 Fragments arranged by size: 1880 1373 688 678 521 465 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 589 619 2298 2328 589 Size: 30 1679 30 4698 Fragments arranged by size: 4698 1679 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 524 2071 2139 2707 2966 524 Size: 1547 68 568 259 3995 Fragments arranged by size: 3995 1547 568 259 68 BseYI C'CCAG_C Cuts at: 1630 2118 3219 3242 3862 4620 5197 5693 1630 Size: 488 1101 23 620 758 577 496 2374 Fragments arranged by size: 2374 1101 758 620 577 496 488 23 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 868 1168 2059 2798 2818 868 Size: 300 891 739 20 4487 Fragments arranged by size: 4487 891 739 300 20 BsiEI CG_ry'CG Cuts at: 104 3979 4025 5233 104 Size: 3875 46 1208 1308 Fragments arranged by size: 3875 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 1589 1831 2360 3451 4229 4419 5211 1589 Size: 242 529 1091 778 190 792 2815 Fragments arranged by size: 2815 1091 792 778 529 242 190 Bsp1286I G_dGCh'C Cuts at: 835 1589 1831 2360 2515 2770 2788 3451 4062 Size: 754 242 529 155 255 18 663 611 Cuts at: 4062 4155 4229 4419 4481 5211 835 Size: 93 74 190 62 730 2061 Fragments arranged by size: 2061 754 730 663 611 529 255 242 190 155 93 74 62 18 BspEI T'CCGG_A Cuts at: 593 1746 5657 593 Size: 1153 3911 1373 Fragments arranged by size: 3911 1373 1153 BspHI T'CATG_A Cuts at: 482 1484 1581 5613 482 Size: 1002 97 4032 1306 Fragments arranged by size: 4032 1306 1002 97 BspMI ACCTGCnnnn'nnnn_ Cuts at: 2087 2465 2847 3487 3701 4003 4384 2087 Size: 378 382 640 214 302 381 4140 Fragments arranged by size: 4140 640 382 381 378 302 214 BsrBI CCG'CTC Cuts at: 50 343 726 4729 4783 6267 6432 50 Size: 293 383 4003 54 1484 165 55 Fragments arranged by size: 4003 1484 383 293 165 55 54 BsrDI GCAATG_nn' Cuts at: 4349 4349 Size: 6437 BsrFI r'CCGG_y Cuts at: 1058 1082 4435 4616 4825 1058 Size: 24 3353 181 209 2670 Fragments arranged by size: 3353 2670 209 181 24 BsrGI T'GTAC_A Cuts at: 392 3071 392 Size: 2679 3758 BssSI C'ACGA_G Cuts at: 4708 5066 4708 Size: 358 6079 BstAPI GCAn_nnn'nTGC Cuts at: 2082 3814 2082 Size: 1732 4705 BstBI TT'CG_AA Cuts at: 4798 4798 Size: 6437 BstXI CCAn_nnnn'nTGG Cuts at: 1300 2815 3040 1300 Size: 1515 225 4697 Fragments arranged by size: 4697 1515 225 BstYI r'GATC_y Cuts at: 1 265 671 905 1692 2567 3357 3462 3929 Size: 264 406 234 787 875 790 105 467 Cuts at: 3929 3952 4287 4533 5545 5631 5643 5989 1 Size: 23 335 246 1012 86 12 346 449 Fragments arranged by size: 1012 875 790 787 467 449 406 346 335 264 246 234 105 86 23 12 Bsu36I CC'TnA_GG Cuts at: 3260 3260 Size: 6437 BtgI C'CryG_G Cuts at: 107 1229 3080 107 Size: 1122 1851 3464 Fragments arranged by size: 3464 1851 1122 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 4387 4568 4731 4387 Size: 181 163 6093 Fragments arranged by size: 6093 181 163 BtsI GCAGTG_nn' Cuts at: 3680 3680 Size: 6437 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 144 179 144 Size: 35 6402 DraI TTT'AAA Cuts at: 3436 3436 Size: 6437 DrdI GACnn_nn'nnGTC Cuts at: 2827 4143 5001 2827 Size: 1316 858 4263 Fragments arranged by size: 4263 1316 858 EaeI y'GGCC_r Cuts at: 4022 4196 4587 4614 5959 4022 Size: 174 391 27 1345 4500 Fragments arranged by size: 4500 1345 391 174 27 EagI C'GGCC_G Cuts at: 4022 4022 Size: 6437 EarI CTCTTCn'nnn_ Cuts at: 223 911 1007 1959 2165 2348 4460 4670 223 Size: 688 96 952 206 183 2112 210 1990 Fragments arranged by size: 2112 1990 952 688 210 206 183 96 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 4645 4955 5101 6247 4645 Size: 310 146 1146 4835 Fragments arranged by size: 4835 1146 310 146 EcoICRI GAG'CTC Cuts at: 3449 3449 Size: 6437 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 244 480 527 586 591 1537 1546 2353 2563 Size: 236 47 59 5 946 9 807 210 Cuts at: 2563 2764 2793 4262 4694 5441 5709 244 Size: 201 29 1469 432 747 268 972 Fragments arranged by size: 1469 972 946 807 747 432 268 236 210 201 59 47 29 9 5 EcoNI CCTnn'n_nnAGG Cuts at: 2981 2981 Size: 6437 EcoO109I rG'GnC_Cy Cuts at: 1179 2511 2523 2766 2767 2820 3165 3399 3576 Size: 1332 12 243 1 53 345 234 177 Cuts at: 3576 1179 Size: 4040 Fragments arranged by size: 4040 1332 345 243 234 177 53 12 1 EcoRI G'AATT_C Cuts at: 2662 3441 2662 Size: 779 5658 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4456 4456 Size: 6437 FspI TGC'GCA Cuts at: 4218 4218 Size: 6437 HaeII r_GCGC'y Cuts at: 764 1779 3871 4119 5141 6120 764 Size: 1015 2092 248 1022 979 1081 Fragments arranged by size: 2092 1081 1022 1015 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1563 1595 2486 2518 3042 3074 3115 3147 4279 Size: 32 891 32 524 32 41 32 1132 Cuts at: 4279 4309 4311 4341 1563 Size: 30 2 30 3659 Fragments arranged by size: 3659 1132 891 524 41 32 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 285 2094 3382 3476 285 Size: 1809 1288 94 3246 Fragments arranged by size: 3246 1809 1288 94 KasI G'GCGC_C Cuts at: 760 1775 3867 4115 760 Size: 1015 2092 248 3082 Fragments arranged by size: 3082 2092 1015 248 KpnI G_GTAC'C Cuts at: 3457 3457 Size: 6437 MluI A'CGCG_T Cuts at: 5870 5870 Size: 6437 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 578 701 1579 5108 5292 578 Size: 123 878 3529 184 1723 Fragments arranged by size: 3529 1723 878 184 123 MscI TGG'CCA Cuts at: 4198 4198 Size: 6437 MslI CAynn'nnrTG Cuts at: 164 371 799 895 1126 1625 1834 1957 3079 Size: 207 428 96 231 499 209 123 1122 Cuts at: 3079 4426 4553 164 Size: 1347 127 2048 Fragments arranged by size: 2048 1347 1122 499 428 231 209 207 127 123 96 MspA1I CmG'CkG Cuts at: 561 2118 3548 3862 4222 5235 5480 561 Size: 1557 1430 314 360 1013 245 1518 Fragments arranged by size: 1557 1518 1430 1013 360 314 245 NaeI GCC'GGC Cuts at: 1084 4618 1084 Size: 3534 2903 NarI GG'CG_CC Cuts at: 761 1776 3868 4116 761 Size: 1015 2092 248 3082 Fragments arranged by size: 3082 2092 1015 248 NcoI C'CATG_G Cuts at: 107 1229 3080 107 Size: 1122 1851 3464 Fragments arranged by size: 3464 1851 1122 NgoMIV G'CCGG_C Cuts at: 1082 4616 1082 Size: 3534 2903 NsiI A_TGCA'T Cuts at: 1520 1520 Size: 6437 NspI r_CATG'y Cuts at: 1522 3490 3990 4897 1522 Size: 1968 500 907 3062 Fragments arranged by size: 3062 1968 907 500 PciI A'CATG_T Cuts at: 4893 4893 Size: 6437 PflMI CCAn_nnn'nTGG Cuts at: 310 664 1898 310 Size: 354 1234 4849 Fragments arranged by size: 4849 1234 354 PfoI T'CCnGG_A Cuts at: 1493 3039 3167 1493 Size: 1546 128 4763 Fragments arranged by size: 4763 1546 128 PmeI GTTT'AAAC Cuts at: 3436 3436 Size: 6437 PmlI CAC'GTG Cuts at: 531 1128 531 Size: 597 5840 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 589 621 5576 5608 589 Size: 32 4955 32 1418 Fragments arranged by size: 4955 1418 32 32 PpuMI rG'GwC_Cy Cuts at: 2820 3165 2820 Size: 345 6092 PshAI GACnn'nnGTC Cuts at: 520 520 Size: 6437 PspOMI G'GGCC_C Cuts at: 2511 2766 2511 Size: 255 6182 PspXI vC'TCGA_Gb Cuts at: 2204 2204 Size: 6437 PstI C_TGCA'G Cuts at: 551 771 1346 1760 2087 2858 3186 3484 4169 Size: 220 575 414 327 771 328 298 685 Cuts at: 4169 551 Size: 2819 Fragments arranged by size: 2819 771 685 575 414 328 327 298 220 PvuI CG_AT'CG Cuts at: 104 104 Size: 6437 PvuII CAG'CTG Cuts at: 2118 3862 4222 2118 Size: 1744 360 4333 Fragments arranged by size: 4333 1744 360 RsrII CG'GwC_CG Cuts at: 4632 4632 Size: 6437 SacI G_AGCT'C Cuts at: 3451 3451 Size: 6437 SalI G'TCGA_C Cuts at: 3380 3474 3380 Size: 94 6343 SapI GCTCTTCn'nnn_ Cuts at: 911 1959 2348 4460 4670 911 Size: 1048 389 2112 210 2678 Fragments arranged by size: 2678 2112 1048 389 210 SbfI CC_TGCA'GG Cuts at: 3484 3484 Size: 6437 ScaI AGT'ACT Cuts at: 1426 2589 5954 1426 Size: 1163 3365 1909 Fragments arranged by size: 3365 1909 1163 SfcI C'TryA_G Cuts at: 33 547 767 1342 1756 2005 2083 2854 3182 Size: 514 220 575 414 249 78 771 328 Cuts at: 3182 3480 4165 5158 5349 33 Size: 298 685 993 191 1121 Fragments arranged by size: 1121 993 771 685 575 514 414 328 298 249 220 191 78 SfoI GGC'GCC Cuts at: 762 1777 3869 4117 762 Size: 1015 2092 248 3082 Fragments arranged by size: 3082 2092 1015 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 6437 SmaI CCC'GGG Cuts at: 1162 1192 3459 1162 Size: 30 2267 4140 Fragments arranged by size: 4140 2267 30 SmlI C'TyrA_G Cuts at: 2204 2833 3160 3592 4507 4999 5261 2204 Size: 629 327 432 915 492 262 3380 Fragments arranged by size: 3380 915 629 492 432 327 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 6437 SphI G_CATG'C Cuts at: 1522 3490 3990 1522 Size: 1968 500 3969 Fragments arranged by size: 3969 1968 500 SspI AAT'ATT Cuts at: 1289 1933 1289 Size: 644 5793 StuI AGG'CCT Cuts at: 571 1150 571 Size: 579 5858 StyI C'CwwG_G Cuts at: 107 336 786 1175 1229 1605 2808 3080 3571 Size: 229 450 389 54 376 1203 272 491 Cuts at: 3571 3925 107 Size: 354 2619 Fragments arranged by size: 2619 1203 491 450 389 376 354 272 229 54 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1431 1516 2079 2375 3140 4020 4153 4612 4821 Size: 85 563 296 765 880 133 459 209 Cuts at: 4821 1431 Size: 3047 Fragments arranged by size: 3047 880 765 563 459 296 209 133 85 TatI w'GTAC_w Cuts at: 392 1424 2480 2587 3071 5952 392 Size: 1032 1056 107 484 2881 877 Fragments arranged by size: 2881 1056 1032 877 484 107 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 449 481 1084 1116 1489 1521 5954 5986 449 Size: 32 603 32 373 32 4433 32 900 Fragments arranged by size: 4433 900 603 373 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 2413 4234 2413 Size: 1821 4616 XbaI T'CTAG_A Cuts at: 3468 3468 Size: 6437 XcmI CCAnnnn_n'nnnnTGG Cuts at: 561 684 2812 3932 561 Size: 123 2128 1120 3066 Fragments arranged by size: 3066 2128 1120 123 XhoI C'TCGA_G Cuts at: 2204 2204 Size: 6437 XmaI C'CCGG_G Cuts at: 1160 1190 3457 1160 Size: 30 2267 4140 Fragments arranged by size: 4140 2267 30 XmnI GAAnn'nnTTC Cuts at: 1391 1391 Size: 6437 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI Enzymes that do not cut: AatII AfeI AflII AscI AvrII BaeI BbvCI BcgI BmtI BsiWI BsmI BsmBI BssHII BstEII BstZ17I ClaI DraIII EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI PacI PasI PsiI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SwaI ZraI