Restriction map for pF1KB9100
 (Linear) (Six-base) MAPSORT of: KIBB9100.seq  Check: 9861  from: 1  to: 3324



KIBB9100.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:10  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1979    3324 
   Size:       1979    1345
 
AccI GT'mk_AC

Cuts at:      0      17    3004    3273    3324 
   Size:         17    2987     269      51

  Fragments arranged by size: 

                2987     269      51      17
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     136    1438    2455    2656    3324 
   Size:        136    1302    1017     201     668

  Fragments arranged by size: 

                1302    1017     668     201     136
 
AflIII A'CryG_T

Cuts at:      0    1394    3324 
   Size:       1394    1930
 
AgeI A'CCGG_T

Cuts at:      0     950    3324 
   Size:        950    2374
 
AhdI GACnn_n'nnGTC

Cuts at:      0    3021    3324 
   Size:       3021     303
 
AleI CACnn'nnGTG

Cuts at:      0      56    3324 
   Size:         56    3268
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1630    3324 
   Size:       1630    1694
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     481     513    3324 
   Size:        481      32    2811

  Fragments arranged by size: 

                2811     481      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     361    1440    1790    3294    3324 
   Size:        361    1079     350    1504      30

  Fragments arranged by size: 

                1504    1079     361     350      30
 
ApaI G_GGCC'C

Cuts at:      0    2407    2662    3324 
   Size:       2407     255     662

  Fragments arranged by size: 

                2407     662     255
 
ApoI r'AATT_y

Cuts at:      0    1891    2122    2554    2614    3324 
   Size:       1891     231     432      60     710

  Fragments arranged by size: 

                1891     710     432     231      60
 
AseI AT'TA_AT

Cuts at:      0     166    3324 
   Size:        166    3158
 
AvaI C'yCGr_G

Cuts at:      0     720    1052    1082    2041    2096    3324 
   Size:        720     332      30     959      55    1228

  Fragments arranged by size: 

                1228     959     720     332      55      30
 
BanI G'GyrC_C

Cuts at:      0     652    1667    3156    3324 
   Size:        652    1015    1489     168

  Fragments arranged by size: 

                1489    1015     652     168
 
BanII G_rGCy'C

Cuts at:      0     727    2407    2662    2680    3324 
   Size:        727    1680     255      18     644

  Fragments arranged by size: 

                1680     727     644     255      18
 
BbeI G_GCGC'C

Cuts at:      0     656    1671    3324 
   Size:        656    1015    1653

  Fragments arranged by size: 

                1653    1015     656
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    2535    2596    3324 
   Size:       2535      61     728

  Fragments arranged by size: 

                2535     728      61
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1542    2872    3324 
   Size:       1542    1330     452

  Fragments arranged by size: 

                1542    1330     452
 
BclI T'GATC_A

Cuts at:      0     296    3324 
   Size:        296    3028
 
BfrBI ATG'CAT

Cuts at:      0    1410    3324 
   Size:       1410    1914
 
BglI GCCn_nnn'nGGC

Cuts at:      0     722    1938    3324 
   Size:        722    1216    1386

  Fragments arranged by size: 

                1386    1216     722
 
BglII A'GATC_T

Cuts at:      0     563    3324 
   Size:        563    2761
 
Bme1580I G_kGCm'C

Cuts at:      0    2407    2662    3324 
   Size:       2407     255     662

  Fragments arranged by size: 

                2407     662     255
 
BmgBI CAC'GTC

Cuts at:      0    1395    3324 
   Size:       1395    1929
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1800    2664    2726    2845    3324 
   Size:       1800     864      62     119     479

  Fragments arranged by size: 

                1800     864     479     119      62
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     672    2368    3324 
   Size:        672    1696     956

  Fragments arranged by size: 

                1696     956     672
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     372     419     478     483    1429    2245    2685    3324 
   Size:        372      47      59       5     946     816     440     639

  Fragments arranged by size: 

                 946     816     639     440     372      59      47       5
            
 
Bpu10I CC'TnA_GC

Cuts at:      0     712    2560    3191    3324 
   Size:        712    1848     631     133

  Fragments arranged by size: 

                1848     712     631     133
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2710    3037    3324 
   Size:       2710     327     287

  Fragments arranged by size: 

                2710     327     287
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    2339    3324 
   Size:       2339     985
 
BsaAI yAC'GTr

Cuts at:      0     423    1020    3324 
   Size:        423     597    2304

  Fragments arranged by size: 

                2304     597     423
 
BsaBI GATnn'nnATC

Cuts at:      0     796    3324 
   Size:        796    2528
 
BsaHI Gr'CG_yC

Cuts at:      0     653    1154    1668    1961    3031    3324 
   Size:        653     501     514     293    1070     293

  Fragments arranged by size: 

                1070     653     514     501     293     293
 
BsaWI w'CCGG_w

Cuts at:      0     485     950    1638    3324 
   Size:        485     465     688    1686

  Fragments arranged by size: 

                1686     688     485     465
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     481     511    2190    2220    3324 
   Size:        481      30    1679      30    1104

  Fragments arranged by size: 

                1679    1104     481      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     416    1963    2031    2599    2858    3324 
   Size:        416    1547      68     568     259     466

  Fragments arranged by size: 

                1547     568     466     416     259      68
 
BseYI C'CCAG_C

Cuts at:      0    1522    2010    3111    3134    3324 
   Size:       1522     488    1101      23     190

  Fragments arranged by size: 

                1522    1101     488     190      23
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     760    1060    1951    2690    2710    3324 
   Size:        760     300     891     739      20     614

  Fragments arranged by size: 

                 891     760     739     614     300      20
 
BsiHKAI G_wGCw'C

Cuts at:      0    1481    1723    2252    3324 
   Size:       1481     242     529    1072

  Fragments arranged by size: 

                1481    1072     529     242
 
Bsp1286I G_dGCh'C

Cuts at:      0     727    1481    1723    2252    2407    2662    2680    3324 
   Size:        727     754     242     529     155     255      18     644

  Fragments arranged by size: 

                 754     727     644     529     255     242     155      18
            
 
BspEI T'CCGG_A

Cuts at:      0     485    1638    3324 
   Size:        485    1153    1686

  Fragments arranged by size: 

                1686    1153     485
 
BspHI T'CATG_A

Cuts at:      0     374    1376    1473    3324 
   Size:        374    1002      97    1851

  Fragments arranged by size: 

                1851    1002     374      97
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1979    2357    2739    3324 
   Size:       1979     378     382     585

  Fragments arranged by size: 

                1979     585     382     378
 
BsrBI CCG'CTC

Cuts at:      0     235     618    3324 
   Size:        235     383    2706

  Fragments arranged by size: 

                2706     383     235
 
BsrFI r'CCGG_y

Cuts at:      0     950     974    3324 
   Size:        950      24    2350

  Fragments arranged by size: 

                2350     950      24
 
BsrGI T'GTAC_A

Cuts at:      0     284    2963    3324 
   Size:        284    2679     361

  Fragments arranged by size: 

                2679     361     284
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1974    3324 
   Size:       1974    1350
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1192    2707    2932    3324 
   Size:       1192    1515     225     392

  Fragments arranged by size: 

                1515    1192     392     225
 
BstYI r'GATC_y

Cuts at:      0     157     563     797    1584    2459    3249    3324 
   Size:        157     406     234     787     875     790      75

  Fragments arranged by size: 

                 875     790     787     406     234     157      75
 
Bsu36I CC'TnA_GG

Cuts at:      0    3152    3324 
   Size:       3152     172
 
BtgI C'CryG_G

Cuts at:      0    1121    2972    3324 
   Size:       1121    1851     352

  Fragments arranged by size: 

                1851    1121     352
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0      36      71    3324 
   Size:         36      35    3253

  Fragments arranged by size: 

                3253      36      35
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    2719    3324 
   Size:       2719     605
 
EarI CTCTTCn'nnn_

Cuts at:      0     115     803     899    1851    2057    2240    3324 
   Size:        115     688      96     952     206     183    1084

  Fragments arranged by size: 

                1084     952     688     206     183     115      96
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     136     372     419     478     483    1429    1438    2245 
   Size:        136     236      47      59       5     946       9     807

Cuts at:   2245    2455    2656    2685    3324 
   Size:        210     201      29     639

  Fragments arranged by size: 

                 946     807     639     236     210     201     136      59
                  47      29       9       5
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    2873    3324 
   Size:       2873     451
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1071    2403    2415    2658    2659    2712    3057    3291 
   Size:       1071    1332      12     243       1      53     345     234

Cuts at:   3291    3324 
   Size:         33

  Fragments arranged by size: 

                1332    1071     345     243     234      53      33      12
                   1
 
EcoRI G'AATT_C

Cuts at:      0    2554    3324 
   Size:       2554     770
 
HaeII r_GCGC'y

Cuts at:      0     656    1671    3324 
   Size:        656    1015    1653

  Fragments arranged by size: 

                1653    1015     656
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1455    1487    2378    2410    2934    2966    3007    3039 
   Size:       1455      32     891      32     524      32      41      32

Cuts at:   3039    3324 
   Size:        285

  Fragments arranged by size: 

                1455     891     524     285      41      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0     177    1986    3274    3324 
   Size:        177    1809    1288      50

  Fragments arranged by size: 

                1809    1288     177      50
 
KasI G'GCGC_C

Cuts at:      0     652    1667    3324 
   Size:        652    1015    1657

  Fragments arranged by size: 

                1657    1015     652
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     470     593    1471    3324 
   Size:        470     123     878    1853

  Fragments arranged by size: 

                1853     878     470     123
 
MslI CAynn'nnrTG

Cuts at:      0      56     263     691     787    1018    1517    1726    1849 
   Size:         56     207     428      96     231     499     209     123

Cuts at:   1849    2971    3324 
   Size:       1122     353

  Fragments arranged by size: 

                1122     499     428     353     231     209     207     123
                  96      56
 
MspA1I CmG'CkG

Cuts at:      0     453    2010    3324 
   Size:        453    1557    1314

  Fragments arranged by size: 

                1557    1314     453
 
NaeI GCC'GGC

Cuts at:      0     976    3324 
   Size:        976    2348
 
NarI GG'CG_CC

Cuts at:      0     653    1668    3324 
   Size:        653    1015    1656

  Fragments arranged by size: 

                1656    1015     653
 
NcoI C'CATG_G

Cuts at:      0    1121    2972    3324 
   Size:       1121    1851     352

  Fragments arranged by size: 

                1851    1121     352
 
NgoMIV G'CCGG_C

Cuts at:      0     974    3324 
   Size:        974    2350
 
NsiI A_TGCA'T

Cuts at:      0    1412    3324 
   Size:       1412    1912
 
NspI r_CATG'y

Cuts at:      0    1414    3324 
   Size:       1414    1910
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     202     556    1790    3324 
   Size:        202     354    1234    1534

  Fragments arranged by size: 

                1534    1234     354     202
 
PfoI T'CCnGG_A

Cuts at:      0    1385    2931    3059    3324 
   Size:       1385    1546     128     265

  Fragments arranged by size: 

                1546    1385     265     128
 
PmlI CAC'GTG

Cuts at:      0     423    1020    3324 
   Size:        423     597    2304

  Fragments arranged by size: 

                2304     597     423
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     481     513    3324 
   Size:        481      32    2811

  Fragments arranged by size: 

                2811     481      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    2712    3057    3324 
   Size:       2712     345     267

  Fragments arranged by size: 

                2712     345     267
 
PshAI GACnn'nnGTC

Cuts at:      0     412    3324 
   Size:        412    2912
 
PspOMI G'GGCC_C

Cuts at:      0    2403    2658    3324 
   Size:       2403     255     666

  Fragments arranged by size: 

                2403     666     255
 
PspXI vC'TCGA_Gb

Cuts at:      0    2096    3324 
   Size:       2096    1228
 
PstI C_TGCA'G

Cuts at:      0     443     663    1238    1652    1979    2750    3078    3324 
   Size:        443     220     575     414     327     771     328     246

  Fragments arranged by size: 

                 771     575     443     414     328     327     246     220
            
 
PvuII CAG'CTG

Cuts at:      0    2010    3324 
   Size:       2010    1314
 
SalI G'TCGA_C

Cuts at:      0    3272    3324 
   Size:       3272      52
 
SapI GCTCTTCn'nnn_

Cuts at:      0     803    1851    2240    3324 
   Size:        803    1048     389    1084

  Fragments arranged by size: 

                1084    1048     803     389
 
ScaI AGT'ACT

Cuts at:      0    1318    2481    3324 
   Size:       1318    1163     843

  Fragments arranged by size: 

                1318    1163     843
 
SfcI C'TryA_G

Cuts at:      0     439     659    1234    1648    1897    1975    2746    3074 
   Size:        439     220     575     414     249      78     771     328

Cuts at:   3074    3324 
   Size:        250

  Fragments arranged by size: 

                 771     575     439     414     328     250     249     220
                  78
 
SfoI GGC'GCC

Cuts at:      0     654    1669    3324 
   Size:        654    1015    1655

  Fragments arranged by size: 

                1655    1015     654
 
SmaI CCC'GGG

Cuts at:      0    1054    1084    3324 
   Size:       1054      30    2240

  Fragments arranged by size: 

                2240    1054      30
 
SmlI C'TyrA_G

Cuts at:      0    2096    2725    3052    3324 
   Size:       2096     629     327     272

  Fragments arranged by size: 

                2096     629     327     272
 
SphI G_CATG'C

Cuts at:      0    1414    3324 
   Size:       1414    1910
 
SspI AAT'ATT

Cuts at:      0    1181    1825    3324 
   Size:       1181     644    1499

  Fragments arranged by size: 

                1499    1181     644
 
StuI AGG'CCT

Cuts at:      0     463    1042    3324 
   Size:        463     579    2282

  Fragments arranged by size: 

                2282     579     463
 
StyI C'CwwG_G

Cuts at:      0     228     678    1067    1121    1497    2700    2972    3324 
   Size:        228     450     389      54     376    1203     272     352

  Fragments arranged by size: 

                1203     450     389     376     352     272     228      54
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1323    1408    1971    2267    3032    3324 
   Size:       1323      85     563     296     765     292

  Fragments arranged by size: 

                1323     765     563     296     292      85
 
TatI w'GTAC_w

Cuts at:      0     284    1316    2372    2479    2963    3324 
   Size:        284    1032    1056     107     484     361

  Fragments arranged by size: 

                1056    1032     484     361     284     107
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     341     373     976    1008    1381    1413    3324 
   Size:        341      32     603      32     373      32    1911

  Fragments arranged by size: 

                1911     603     373     341      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    2305    3324 
   Size:       2305    1019
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     453     576    2704    3324 
   Size:        453     123    2128     620

  Fragments arranged by size: 

                2128     620     453     123
 
XhoI C'TCGA_G

Cuts at:      0    2096    3324 
   Size:       2096    1228
 
XmaI C'CCGG_G

Cuts at:      0    1052    1082    3324 
   Size:       1052      30    2242

  Fragments arranged by size: 

                2242    1052      30
 
XmnI GAAnn'nnTTC

Cuts at:      0    1283    3324 
   Size:       1283    2041
 

 Enzymes that do cut:

     AarI     AccI     AcuI   AflIII     AgeI     AhdI     AleI     AlfI
     AloI    AlwNI     ApaI     ApoI     AseI     AvaI     BanI    BanII
     BbeI     BbsI    BciVI     BclI    BfrBI     BglI    BglII Bme1580I
    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BstAPI
    BstXI    BstYI   Bsu36I     BtgI    CspCI     DrdI     EarI  Eco57MI
    EcoNI EcoO109I    EcoRI    HaeII    Hin4I   HincII     KasI     MmeI
     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NsiI     NspI
    PflMI     PfoI     PmlI     PpiI    PpuMI    PshAI   PspOMI    PspXI
     PstI    PvuII     SalI     SapI     ScaI     SfcI     SfoI     SmaI
     SmlI     SphI     SspI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XcmI     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII    ApaLI     AscI    AsiSI
    AvrII     BaeI    BamHI    BbvCI     BcgI     BlpI     BmtI    BsiEI
    BsiWI     BsmI    BsmBI    BsrDI   BssHII    BssSI    BstBI   BstEII
  BstZ17I    BtgZI     BtsI     ClaI     DraI   DraIII     EaeI     EagI
     EciI  EcoICRI    EcoRV     FalI     FseI     FspI    FspAI  HindIII
     HpaI     KpnI     MfeI     MluI     MscI     NdeI     NheI     NotI
     NruI     PacI     PasI     PciI     PmeI     PsiI     PsrI     PvuI
    RsrII     SacI    SacII    SanDI     SbfI    SexAI     SfiI     SgfI
    SgrAI    SnaBI     SpeI     SrfI     SwaI     XbaI     ZraI
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