Restriction map for pF1KB8999
 (Circular) (Six-base) MAPSORT of: pF1KB8999.seq  Check: 4508  from: 1  to: 4184



pF1KB8999  4184 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:30  ..

AccI GT'mk_AC

Cuts at:   1222    1222 
   Size:       4184
 
Acc65I G'GTAC_C

Cuts at:   1200    1200 
   Size:       4184
 
AclI AA'CG_TT

Cuts at:   4024    4024 
   Size:       4184
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   2009    2441    3188    3456    2009 
   Size:        432     747     268    2737

  Fragments arranged by size: 

                2737     747     432     268
 
AflIII A'CryG_T

Cuts at:    926    1176    1536    2640    3617     926 
   Size:        250     360    1104     977    1493

  Fragments arranged by size: 

                1493    1104     977     360     250
 
AgeI A'CCGG_T

Cuts at:   2572    2572 
   Size:       4184
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    115     147     115 
   Size:         32    4152
 
AlwNI CAG_nnn'CTG

Cuts at:    494    1489    3056     494 
   Size:        995    1567    1622

  Fragments arranged by size: 

                1622    1567     995
 
ApaLI G'TGCA_C

Cuts at:    403    2954     403 
   Size:       2551    1633
 
ApoI r'AATT_y

Cuts at:   1188    3537    3793    1188 
   Size:       2349     256    1579

  Fragments arranged by size: 

                2349    1579     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4184
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4184
 
AvaI C'yCGr_G

Cuts at:   1204    1204 
   Size:       4184
 
BamHI G'GATC_C

Cuts at:   1001    1209    1001 
   Size:        208    3976
 
BanI G'GyrC_C

Cuts at:    419     727    1200    1614    1862    1897     419 
   Size:        308     473     414     248      35    2706

  Fragments arranged by size: 

                2706     473     414     308     248      35
 
BanII G_rGCy'C

Cuts at:    805    1025    1198    2228     805 
   Size:        220     173    1030    2761

  Fragments arranged by size: 

                2761    1030     220     173
 
BbeI G_GCGC'C

Cuts at:    423     731    1618    1866     423 
   Size:        308     887     248    2741

  Fragments arranged by size: 

                2741     887     308     248
 
BbsI GAAGACnn'nnnn_

Cuts at:    213     817     213 
   Size:        604    3580
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    418     452     418 
   Size:         34    4150
 
BciVI GTATCCnnnnn_n'

Cuts at:   2082    2843    4184    2082 
   Size:        761    1341    2082

  Fragments arranged by size: 

                2082    1341     761
 
BclI T'GATC_A

Cuts at:   1704    1704 
   Size:       4184
 
BglII A'GATC_T

Cuts at:    773    1699    3736     773 
   Size:        926    2037    1221

  Fragments arranged by size: 

                2037    1221     926
 
BlpI GC'TnA_GC

Cuts at:    394    1296     394 
   Size:        902    3282
 
Bme1580I G_kGCm'C

Cuts at:    332     407     609    1809    1902    2958     332 
   Size:         75     202    1200      93    1056    1558

  Fragments arranged by size: 

                1558    1200    1056     202      93      75
 
BmgBI CAC'GTC

Cuts at:    471     471 
   Size:       4184
 
BmrI ACTGGGnnnn_n'

Cuts at:   1396    1477    1812    3956    1396 
   Size:         81     335    2144    1624

  Fragments arranged by size: 

                2144    1624     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    678    2551     678 
   Size:       1873    2311
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    789     789 
   Size:       4184
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1360    2239    2731    3029    1360 
   Size:        879     492     298    2515

  Fragments arranged by size: 

                2515     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    157    3888     157 
   Size:       3731     453
 
BsaAI yAC'GTr

Cuts at:    929    1537     929 
   Size:        608    3576
 
BsaBI GATnn'nnATC

Cuts at:      6    1698    3735       6 
   Size:       1692    2037     455

  Fragments arranged by size: 

                2037    1692     455
 
BsaHI Gr'CG_yC

Cuts at:    420     728    1615    1863    2565    4061     420 
   Size:        308     887     248     702    1496     543

  Fragments arranged by size: 

                1496     887     702     543     308     248
 
BsaWI w'CCGG_w

Cuts at:   1373    1894    2572    2846    2993    3404    1373 
   Size:        521     678     274     147     411    2153

  Fragments arranged by size: 

                2153     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    115     145    1081    1111     115 
   Size:         30     936      30    3188

  Fragments arranged by size: 

                3188     936      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    691     691 
   Size:       4184
 
BseYI C'CCAG_C

Cuts at:    199     246     647    1609    2367    2944    3440     199 
   Size:         47     401     962     758     577     496     943

  Fragments arranged by size: 

                 962     943     758     577     496     401      47
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    465     465 
   Size:       4184
 
BsiEI CG_ry'CG

Cuts at:    104    1726    1772    2980     104 
   Size:       1622      46    1208    1308

  Fragments arranged by size: 

                1622    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    407     758     995    1025    1198    1976    2166    2958     407 
   Size:        351     237      30     173     778     190     792    1633

  Fragments arranged by size: 

                1633     792     778     351     237     190     173      30
            
 
BsmBI CGTCTCn'nnnn_

Cuts at:    363     582     649     828     363 
   Size:        219      67     179    3719

  Fragments arranged by size: 

                3719     219     179      67
 
Bsp1286I G_dGCh'C

Cuts at:    332     407     609     758     805     995    1025    1198    1809 
   Size:         75     202     149      47     190      30     173     611

Cuts at:   1809    1902    1976    2166    2228    2958     332 
   Size:         93      74     190      62     730    1558

  Fragments arranged by size: 

                1558     730     611     202     190     190     173     149
                  93      75      74      62      47      30
 
BspEI T'CCGG_A

Cuts at:   3404    3404 
   Size:       4184
 
BspHI T'CATG_A

Cuts at:    587    3360     587 
   Size:       2773    1411
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    200     290     746    1234    1448    1750    2131     200 
   Size:         90     456     488     214     302     381    2253

  Fragments arranged by size: 

                2253     488     456     381     302     214      90
 
BsrBI CCG'CTC

Cuts at:     50     744    2476    2530    4014    4179      50 
   Size:        694    1732      54    1484     165      55

  Fragments arranged by size: 

                1732    1484     694     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2096    2096 
   Size:       4184
 
BsrFI r'CCGG_y

Cuts at:   2182    2363    2572    2182 
   Size:        181     209    3794

  Fragments arranged by size: 

                3794     209     181
 
BssSI C'ACGA_G

Cuts at:    406    2455    2813     406 
   Size:       2049     358    1777

  Fragments arranged by size: 

                2049    1777     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1561    1561 
   Size:       4184
 
BstBI TT'CG_AA

Cuts at:   2545    2545 
   Size:       4184
 
BstXI CCAn_nnnn'nTGG

Cuts at:    516     516 
   Size:       4184
 
BstYI r'GATC_y

Cuts at:      1     773    1001    1209    1676    1699    2034    2280    3292 
   Size:        772     228     208     467      23     335     246    1012

Cuts at:   3292    3378    3390    3736       1 
   Size:         86      12     346     449

  Fragments arranged by size: 

                1012     772     467     449     346     335     246     228
                 208      86      23      12
 
BtgI C'CryG_G

Cuts at:    107     350     107 
   Size:        243    3941
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2134    2315    2478    2134 
   Size:        181     163    3840

  Fragments arranged by size: 

                3840     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1427    1427 
   Size:       4184
 
DraI TTT'AAA

Cuts at:   1183    1183 
   Size:       4184
 
DraIII CAC_nnn'GTG

Cuts at:    327     327 
   Size:       4184
 
DrdI GACnn_nn'nnGTC

Cuts at:   1890    2748    1890 
   Size:        858    3326
 
EaeI y'GGCC_r

Cuts at:    353     374     485     506    1769    1943    2334    2361    3706 
   Size:         21     111      21    1263     174     391      27    1345

Cuts at:   3706     353 
   Size:        831

  Fragments arranged by size: 

                1345    1263     831     391     174     111      27      21
                  21
 
EagI C'GGCC_G

Cuts at:   1769    1769 
   Size:       4184
 
EarI CTCTTCn'nnn_

Cuts at:    763    2207    2417     763 
   Size:       1444     210    2530

  Fragments arranged by size: 

                2530    1444     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    880    2392    2702    2848    3994     880 
   Size:       1512     310     146    1146    1070

  Fragments arranged by size: 

                1512    1146    1070     310     146
 
EcoICRI GAG'CTC

Cuts at:   1023    1196    1023 
   Size:        173    4011
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    789    2009    2441    3188    3456     789 
   Size:       1220     432     747     268    1517

  Fragments arranged by size: 

                1517    1220     747     432     268
 
EcoO109I rG'GnC_Cy

Cuts at:   1323    1323 
   Size:       4184
 
EcoRI G'AATT_C

Cuts at:   1188    1188 
   Size:       4184
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1179    2203    1179 
   Size:       1024    3160
 
FspI TGC'GCA

Cuts at:   1965    1965 
   Size:       4184
 
HaeII r_GCGC'y

Cuts at:    423     731    1151    1618    1866    2888    3867     423 
   Size:        308     420     467     248    1022     979     740

  Fragments arranged by size: 

                1022     979     740     467     420     308     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   2026    2056    2058    2088    2026 
   Size:         30       2      30    4122

  Fragments arranged by size: 

                4122      30      30       2
 
HincII GTy'rAC

Cuts at:   1223    1223 
   Size:       4184
 
KasI G'GCGC_C

Cuts at:    419     727    1614    1862     419 
   Size:        308     887     248    2741

  Fragments arranged by size: 

                2741     887     308     248
 
KpnI G_GTAC'C

Cuts at:   1204    1204 
   Size:       4184
 
MluI A'CGCG_T

Cuts at:   3617    3617 
   Size:       4184
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    418     605    1118    2855    3039     418 
   Size:        187     513    1737     184    1563

  Fragments arranged by size: 

                1737    1563     513     187     184
 
MscI TGG'CCA

Cuts at:   1945    1945 
   Size:       4184
 
MslI CAynn'nnrTG

Cuts at:    982    2173    2300     982 
   Size:       1191     127    2866

  Fragments arranged by size: 

                2866    1191     127
 
MspA1I CmG'CkG

Cuts at:    393     425     616     636     951    1295    1609    1969    2982 
   Size:         32     191      20     315     344     314     360    1013

Cuts at:   2982    3227     393 
   Size:        245    1350

  Fragments arranged by size: 

                1350    1013     360     344     315     314     245     191
                  32      20
 
NaeI GCC'GGC

Cuts at:   2365    2365 
   Size:       4184
 
NarI GG'CG_CC

Cuts at:    420     728    1615    1863     420 
   Size:        308     887     248    2741

  Fragments arranged by size: 

                2741     887     308     248
 
NcoI C'CATG_G

Cuts at:    107     350     107 
   Size:        243    3941
 
NgoMIV G'CCGG_C

Cuts at:   2363    2363 
   Size:       4184
 
NspI r_CATG'y

Cuts at:   1237    1737    2644    1237 
   Size:        500     907    2777

  Fragments arranged by size: 

                2777     907     500
 
PciI A'CATG_T

Cuts at:   2640    2640 
   Size:       4184
 
PflMI CCAn_nnn'nTGG

Cuts at:    339     494     339 
   Size:        155    4029
 
PfoI T'CCnGG_A

Cuts at:    805     805 
   Size:       4184
 
PmeI GTTT'AAAC

Cuts at:   1183    1183 
   Size:       4184
 
PmlI CAC'GTG

Cuts at:    929     929 
   Size:       4184
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    115     147    3323    3355     115 
   Size:         32    3176      32     944

  Fragments arranged by size: 

                3176     944      32      32
 
PstI C_TGCA'G

Cuts at:   1231    1916    1231 
   Size:        685    3499
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4184
 
PvuII CAG'CTG

Cuts at:   1609    1969    1609 
   Size:        360    3824
 
RsrII CG'GwC_CG

Cuts at:    888    2379     888 
   Size:       1491    2693
 
SacI G_AGCT'C

Cuts at:   1025    1198    1025 
   Size:        173    4011
 
SalI G'TCGA_C

Cuts at:   1221    1221 
   Size:       4184
 
SapI GCTCTTCn'nnn_

Cuts at:    763    2207    2417     763 
   Size:       1444     210    2530

  Fragments arranged by size: 

                2530    1444     210
 
SbfI CC_TGCA'GG

Cuts at:   1231    1231 
   Size:       4184
 
ScaI AGT'ACT

Cuts at:   3701    3701 
   Size:       4184
 
SexAI A'CCwGG_T

Cuts at:    338     338 
   Size:       4184
 
SfcI C'TryA_G

Cuts at:     33    1227    1912    2905    3096      33 
   Size:       1194     685     993     191    1121

  Fragments arranged by size: 

                1194    1121     993     685     191
 
SfoI GGC'GCC

Cuts at:    421     729    1616    1864     421 
   Size:        308     887     248    2741

  Fragments arranged by size: 

                2741     887     308     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4184
 
SmaI CCC'GGG

Cuts at:   1206    1206 
   Size:       4184
 
SmlI C'TyrA_G

Cuts at:   1339    2254    2746    3008    1339 
   Size:        915     492     262    2515

  Fragments arranged by size: 

                2515     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4184
 
SphI G_CATG'C

Cuts at:   1237    1737    1237 
   Size:        500    3684
 
StyI C'CwwG_G

Cuts at:    107     350    1318    1672     107 
   Size:        243     968     354    2619

  Fragments arranged by size: 

                2619     968     354     243
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    207     843    1767    1900    2359    2568     207 
   Size:        636     924     133     459     209    1823

  Fragments arranged by size: 

                1823     924     636     459     209     133
 
TatI w'GTAC_w

Cuts at:    764    3699     764 
   Size:       2935    1249
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    397     429    3701    3733     397 
   Size:         32    3272      32     848

  Fragments arranged by size: 

                3272     848      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1981    1981 
   Size:       4184
 
XbaI T'CTAG_A

Cuts at:   1215    1215 
   Size:       4184
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    795    1679     795 
   Size:        884    3300
 
XmaI C'CCGG_G

Cuts at:   1204    1204 
   Size:       4184
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AloI    AlwNI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI     BbsI     BcgI    BciVI     BclI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BssSI   BstAPI    BstBI    BstXI    BstYI     BtgI    BtgZI     BtsI
     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I   HincII
     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI   NgoMIV     NspI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI     PstI     PvuI    PvuII    RsrII     SacI     SalI
     SapI     SbfI     ScaI    SexAI     SfcI     SfoI     SgfI     SmaI
     SmlI     SpeI     SphI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     ApaI
     AscI    AvrII     BaeI    BbvCI    BfrBI     BglI     BmtI   Bpu10I
    BsiWI     BsmI    BsrGI   BssHII   BstEII  BstZ17I   Bsu36I     ClaI
    CspCI    EcoNI    EcoRV     FseI    FspAI  HindIII     HpaI     MfeI
     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI    SacII    SanDI     SfiI
    SgrAI    SnaBI     SrfI     SspI     StuI     SwaI     XhoI     XmnI
     ZraI
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