(Linear) (Six-base) MAPSORT of: KIBB8999.seq Check: 366 from: 1 to: 1071 KIBB8999.orf With 182 enzymes: SgfI * March 3, 2008 11:09 .. AflIII A'CryG_T Cuts at: 0 818 1071 Size: 818 253 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 7 39 1071 Size: 7 32 1032 Fragments arranged by size: 1032 32 7 AlwNI CAG_nnn'CTG Cuts at: 0 386 1071 Size: 386 685 ApaLI G'TGCA_C Cuts at: 0 295 1071 Size: 295 776 BamHI G'GATC_C Cuts at: 0 893 1071 Size: 893 178 BanI G'GyrC_C Cuts at: 0 311 619 1071 Size: 311 308 452 Fragments arranged by size: 452 311 308 BanII G_rGCy'C Cuts at: 0 697 917 1071 Size: 697 220 154 Fragments arranged by size: 697 220 154 BbeI G_GCGC'C Cuts at: 0 315 623 1071 Size: 315 308 448 Fragments arranged by size: 448 315 308 BbsI GAAGACnn'nnnn_ Cuts at: 0 105 709 1071 Size: 105 604 362 Fragments arranged by size: 604 362 105 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 310 344 1071 Size: 310 34 727 Fragments arranged by size: 727 310 34 BglII A'GATC_T Cuts at: 0 665 1071 Size: 665 406 BlpI GC'TnA_GC Cuts at: 0 286 1071 Size: 286 785 Bme1580I G_kGCm'C Cuts at: 0 224 299 501 1071 Size: 224 75 202 570 Fragments arranged by size: 570 224 202 75 BmgBI CAC'GTC Cuts at: 0 363 1071 Size: 363 708 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 570 1071 Size: 570 501 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 681 1071 Size: 681 390 BsaI GGTCTCn'nnnn_ Cuts at: 0 49 1071 Size: 49 1022 BsaAI yAC'GTr Cuts at: 0 821 1071 Size: 821 250 BsaHI Gr'CG_yC Cuts at: 0 312 620 1071 Size: 312 308 451 Fragments arranged by size: 451 312 308 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 7 37 973 1003 1071 Size: 7 30 936 30 68 Fragments arranged by size: 936 68 30 30 7 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 583 1071 Size: 583 488 BseYI C'CCAG_C Cuts at: 0 91 138 539 1071 Size: 91 47 401 532 Fragments arranged by size: 532 401 91 47 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 357 1071 Size: 357 714 BsiHKAI G_wGCw'C Cuts at: 0 299 650 887 917 1071 Size: 299 351 237 30 154 Fragments arranged by size: 351 299 237 154 30 BsmBI CGTCTCn'nnnn_ Cuts at: 0 255 474 541 720 1071 Size: 255 219 67 179 351 Fragments arranged by size: 351 255 219 179 67 Bsp1286I G_dGCh'C Cuts at: 0 224 299 501 650 697 887 917 1071 Size: 224 75 202 149 47 190 30 154 Fragments arranged by size: 224 202 190 154 149 75 47 30 BspHI T'CATG_A Cuts at: 0 479 1071 Size: 479 592 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 92 182 638 1071 Size: 92 90 456 433 Fragments arranged by size: 456 433 92 90 BsrBI CCG'CTC Cuts at: 0 636 1071 Size: 636 435 BssSI C'ACGA_G Cuts at: 0 298 1071 Size: 298 773 BstXI CCAn_nnnn'nTGG Cuts at: 0 408 1071 Size: 408 663 BstYI r'GATC_y Cuts at: 0 665 893 1071 Size: 665 228 178 Fragments arranged by size: 665 228 178 BtgI C'CryG_G Cuts at: 0 242 1071 Size: 242 829 DraIII CAC_nnn'GTG Cuts at: 0 219 1071 Size: 219 852 EaeI y'GGCC_r Cuts at: 0 245 266 377 398 1071 Size: 245 21 111 21 673 Fragments arranged by size: 673 245 111 21 21 EarI CTCTTCn'nnn_ Cuts at: 0 655 1071 Size: 655 416 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 772 1071 Size: 772 299 EcoICRI GAG'CTC Cuts at: 0 915 1071 Size: 915 156 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 681 1071 Size: 681 390 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1071 Size: 1071 HaeII r_GCGC'y Cuts at: 0 315 623 1043 1071 Size: 315 308 420 28 Fragments arranged by size: 420 315 308 28 KasI G'GCGC_C Cuts at: 0 311 619 1071 Size: 311 308 452 Fragments arranged by size: 452 311 308 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 310 497 1010 1071 Size: 310 187 513 61 Fragments arranged by size: 513 310 187 61 MslI CAynn'nnrTG Cuts at: 0 874 1071 Size: 874 197 MspA1I CmG'CkG Cuts at: 0 285 317 508 528 843 1071 Size: 285 32 191 20 315 228 Fragments arranged by size: 315 285 228 191 32 20 NarI GG'CG_CC Cuts at: 0 312 620 1071 Size: 312 308 451 Fragments arranged by size: 451 312 308 NcoI C'CATG_G Cuts at: 0 242 1071 Size: 242 829 PflMI CCAn_nnn'nTGG Cuts at: 0 231 386 1071 Size: 231 155 685 Fragments arranged by size: 685 231 155 PfoI T'CCnGG_A Cuts at: 0 697 1071 Size: 697 374 PmlI CAC'GTG Cuts at: 0 821 1071 Size: 821 250 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 7 39 1071 Size: 7 32 1032 Fragments arranged by size: 1032 32 7 RsrII CG'GwC_CG Cuts at: 0 780 1071 Size: 780 291 SacI G_AGCT'C Cuts at: 0 917 1071 Size: 917 154 SapI GCTCTTCn'nnn_ Cuts at: 0 655 1071 Size: 655 416 SexAI A'CCwGG_T Cuts at: 0 230 1071 Size: 230 841 SfoI GGC'GCC Cuts at: 0 313 621 1071 Size: 313 308 450 Fragments arranged by size: 450 313 308 StyI C'CwwG_G Cuts at: 0 242 1071 Size: 242 829 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 99 735 1071 Size: 99 636 336 Fragments arranged by size: 636 336 99 TatI w'GTAC_w Cuts at: 0 656 1071 Size: 656 415 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 289 321 1071 Size: 289 32 750 Fragments arranged by size: 750 289 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 687 1071 Size: 687 384 Enzymes that do cut: AflIII AloI AlwNI ApaLI BamHI BanI BanII BbeI BbsI BcgI BglII BlpI Bme1580I BmgBI BplI BpmI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BssSI BstXI BstYI BtgI DraIII EaeI EarI EciI EcoICRI Eco57MI FalI HaeII KasI MmeI MslI MspA1I NarI NcoI PflMI PfoI PmlI PpiI RsrII SacI SapI SexAI SfoI StyI TaqII TatI TstI XcmI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AgeI AhdI AleI AlfI ApaI ApoI AscI AseI AsiSI AvaI AvrII BaeI BbvCI BciVI BclI BfrBI BglI BmrI BmtI Bpu10I BpuEI BsaBI BsaWI BsiEI BsiWI BsmI BspEI BsrDI BsrFI BsrGI BssHII BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DrdI EagI EcoNI EcoO109I EcoRI EcoRV FseI FspI FspAI Hin4I HincII HindIII HpaI KpnI MfeI MluI MscI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PmeI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI XhoI XmaI XmnI ZraI